Laser Diffraction

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Ján Horák - One of the best experts on this subject based on the ideXlab platform.

Richard L Lieber - One of the best experts on this subject based on the ideXlab platform.

  • Polarization gating enables sarcomere length measurements by Laser Diffraction in fibrotic muscle
    Journal of biomedical optics, 2014
    Co-Authors: Kevin W. Young, Sudarshan Dayanidhi, Richard L Lieber
    Abstract:

    Sarcomere length is a key parameter commonly measured in muscle physiology since it dictates striated muscle active force. Laser Diffraction (LD)–based measurements of sarcomere length are time-efficient and sample a greater number of sarcomeres compared with traditional microscopy–based techniques. However, a limitation to LD techniques is that signal quality is severely degraded by scattering events as photons propagate through tissue. Consequently, sarcomere length measurements are unattainable when the number of scattering events is sufficiently large in muscle tissue with a high scattering probability. This occurs in fibrotic skeletal muscle seen in muscular dystrophies and secondary to tissue trauma, thus eliminating the use of LD to study these skeletal muscle ailments. Here, we utilize polarization gating to extract diffracted signals that are buried in noise created by scattering. Importantly, we demonstrate that polarization-gated Laser Diffraction (PGLD) enables sarcomere length measurements in muscles from chronically immobilized mice hind limbs; these muscles have a substantial increase of intramuscular connective tissue that scatter light and disable sarcomere length measurements by traditional LD. Further, we compare PGLD sarcomere lengths to those measured by bright field (BF) and confocal microscopy as positive controls and reveal a significant bias of BF but not of confocal microscopy.

Merrill Seymour Goldenberg - One of the best experts on this subject based on the ideXlab platform.

S Chandra - One of the best experts on this subject based on the ideXlab platform.

  • adequacy of Laser Diffraction for soil particle size analysis
    PLOS ONE, 2017
    Co-Authors: Peter Fisher, Craig Aumann, Kohleth Chia, Nick Ohalloran, S Chandra
    Abstract:

    Sedimentation has been a standard methodology for particle size analysis since the early 1900s. In recent years Laser Diffraction is beginning to replace sedimentation as the prefered technique in some industries, such as marine sediment analysis. However, for the particle size analysis of soils, which have a diverse range of both particle size and shape, Laser Diffraction still requires evaluation of its reliability. In this study, the sedimentation based sieve plummet balance method and the Laser Diffraction method were used to measure the particle size distribution of 22 soil samples representing four contrasting Australian Soil Orders. Initially, a precise wet riffling methodology was developed capable of obtaining representative samples within the recommended obscuration range for Laser Diffraction. It was found that repeatable results were obtained even if measurements were made at the extreme ends of the manufacturer’s recommended obscuration range. Results from statistical analysis suggested that the use of sample pretreatment to remove soil organic carbon (and possible traces of calcium-carbonate content) made minor differences to the Laser Diffraction particle size distributions compared to no pretreatment. These differences were found to be marginally statistically significant in the Podosol topsoil and Vertosol subsoil. There are well known reasons why sedimentation methods may be considered to ‘overestimate’ plate-like clay particles, while Laser Diffraction will ‘underestimate’ the proportion of clay particles. In this study we used Lin’s concordance correlation coefficient to determine the equivalence of Laser Diffraction and sieve plummet balance results. The results suggested that the Laser Diffraction equivalent thresholds corresponding to the sieve plummet balance cumulative particle sizes of < 2 μm, < 20 μm, and < 200 μm, were < 9 μm, < 26 μm, < 275 μm respectively. The many advantages of Laser Diffraction for soil particle size analysis, and the empirical results of this study, suggest that deployment of Laser Diffraction as a standard test procedure can provide reliable results, provided consistent sample preparation is used.

Dušan Igaz - One of the best experts on this subject based on the ideXlab platform.