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Carla Mucignatcaretta - One of the best experts on this subject based on the ideXlab platform.
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outcome of bovine herpesvirus 4 infection following direct viral injection in the Lateral Ventricle of the mouse brain
Microbes and Infection, 2006Co-Authors: Gaetano Donofrio, C. F. Flammini, Sandro Cavirani, Andrea Cavaggioni, Michela Bondi, Carla MucignatcarettaAbstract:Abstract A recombinant bovine herpesvirus 4 (BoHV-4EGFPΔTK), obtained by the insertion of an EGFP gene into the TK locus of DN 599 BoHV-4 strain, was injected into the Lateral Ventricle of the brain of mice and a clinical score was evaluated for 90 days. Although BoHV-4 was not neuro-pathogenic, BoHV-4EGFPΔTK transduction capability was analyzed. EGFP expression was localized in close proximity to the border of the Ventricles and EGFP-positive cells were found to co-localize with ependymal cells. Although most of the cells had a polarized morphology, they were not neurons. EGFP-positive cells were seen to spread in tangentially oriented rows within the rostral migratory stream (RMS). Co-localization of EGFP signal with anti-GFAP antibody showed that they were glial cells. EGFP-positive cells were observed until 31 days post-injection and then disappeared completely. Virus isolation was possible at an early post-injection time (3 days), but then virus titer was below the detection limits at later times. Viral DNA, however, could be detected until 21 days post-injection. Thus, in this report we showed that (i) BoHV-4EGFPΔTK did not replicate in the mouse brain, (ii) is not pathogenic and (iii) gene transfer can be obtained in long-lived cells belonging to the RMS after BoHV-4EGFPΔTK injection within the Lateral Ventricle.
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outcome of bovine herpesvirus 4 infection following direct viral injection in the Lateral Ventricle of the mouse brain
Microbes and Infection, 2006Co-Authors: Gaetano Donofrio, C. F. Flammini, Sandro Cavirani, Andrea Cavaggioni, Michela Bondi, Carla MucignatcarettaAbstract:A recombinant bovine herpesvirus 4 (BoHV-4EGFPDeltaTK), obtained by the insertion of an EGFP gene into the TK locus of DN 599 BoHV-4 strain, was injected into the Lateral Ventricle of the brain of mice and a clinical score was evaluated for 90 days. Although BoHV-4 was not neuro-pathogenic, BoHV-4EGFPDeltaTK transduction capability was analyzed. EGFP expression was localized in close proximity to the border of the Ventricles and EGFP-positive cells were found to co-localize with ependymal cells. Although most of the cells had a polarized morphology, they were not neurons. EGFP-positive cells were seen to spread in tangentially oriented rows within the rostral migratory stream (RMS). Co-localization of EGFP signal with anti-GFAP antibody showed that they were glial cells. EGFP-positive cells were observed until 31 days post-injection and then disappeared completely. Virus isolation was possible at an early post-injection time (3 days), but then virus titer was below the detection limits at later times. Viral DNA, however, could be detected until 21 days post-injection. Thus, in this report we showed that (i) BoHV-4EGFPDeltaTK did not replicate in the mouse brain, (ii) is not pathogenic and (iii) gene transfer can be obtained in long-lived cells belonging to the RMS after BoHV-4EGFPDeltaTK injection within the Lateral Ventricle.
Gaetano Donofrio - One of the best experts on this subject based on the ideXlab platform.
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outcome of bovine herpesvirus 4 infection following direct viral injection in the Lateral Ventricle of the mouse brain
Microbes and Infection, 2006Co-Authors: Gaetano Donofrio, C. F. Flammini, Sandro Cavirani, Andrea Cavaggioni, Michela Bondi, Carla MucignatcarettaAbstract:Abstract A recombinant bovine herpesvirus 4 (BoHV-4EGFPΔTK), obtained by the insertion of an EGFP gene into the TK locus of DN 599 BoHV-4 strain, was injected into the Lateral Ventricle of the brain of mice and a clinical score was evaluated for 90 days. Although BoHV-4 was not neuro-pathogenic, BoHV-4EGFPΔTK transduction capability was analyzed. EGFP expression was localized in close proximity to the border of the Ventricles and EGFP-positive cells were found to co-localize with ependymal cells. Although most of the cells had a polarized morphology, they were not neurons. EGFP-positive cells were seen to spread in tangentially oriented rows within the rostral migratory stream (RMS). Co-localization of EGFP signal with anti-GFAP antibody showed that they were glial cells. EGFP-positive cells were observed until 31 days post-injection and then disappeared completely. Virus isolation was possible at an early post-injection time (3 days), but then virus titer was below the detection limits at later times. Viral DNA, however, could be detected until 21 days post-injection. Thus, in this report we showed that (i) BoHV-4EGFPΔTK did not replicate in the mouse brain, (ii) is not pathogenic and (iii) gene transfer can be obtained in long-lived cells belonging to the RMS after BoHV-4EGFPΔTK injection within the Lateral Ventricle.
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outcome of bovine herpesvirus 4 infection following direct viral injection in the Lateral Ventricle of the mouse brain
Microbes and Infection, 2006Co-Authors: Gaetano Donofrio, C. F. Flammini, Sandro Cavirani, Andrea Cavaggioni, Michela Bondi, Carla MucignatcarettaAbstract:A recombinant bovine herpesvirus 4 (BoHV-4EGFPDeltaTK), obtained by the insertion of an EGFP gene into the TK locus of DN 599 BoHV-4 strain, was injected into the Lateral Ventricle of the brain of mice and a clinical score was evaluated for 90 days. Although BoHV-4 was not neuro-pathogenic, BoHV-4EGFPDeltaTK transduction capability was analyzed. EGFP expression was localized in close proximity to the border of the Ventricles and EGFP-positive cells were found to co-localize with ependymal cells. Although most of the cells had a polarized morphology, they were not neurons. EGFP-positive cells were seen to spread in tangentially oriented rows within the rostral migratory stream (RMS). Co-localization of EGFP signal with anti-GFAP antibody showed that they were glial cells. EGFP-positive cells were observed until 31 days post-injection and then disappeared completely. Virus isolation was possible at an early post-injection time (3 days), but then virus titer was below the detection limits at later times. Viral DNA, however, could be detected until 21 days post-injection. Thus, in this report we showed that (i) BoHV-4EGFPDeltaTK did not replicate in the mouse brain, (ii) is not pathogenic and (iii) gene transfer can be obtained in long-lived cells belonging to the RMS after BoHV-4EGFPDeltaTK injection within the Lateral Ventricle.
Arnau Benet - One of the best experts on this subject based on the ideXlab platform.
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new endoscopic route to the temporal horn of the Lateral Ventricle surgical simulation and morphometric assessment
Journal of Neurosurgery, 2014Co-Authors: Jose Juan Gonzalez Sanchez, Jordina Rincontorroella, Alberto Pratsgalino, Matteo De Notaris, Joan Berenguer, Enrique Ferrer Rodriguez, Arnau BenetAbstract:Object The temporal horn of the Lateral Ventricle is a complex structure affected by specific pathological conditions. Current approaches to the temporal horn involve a certain amount of corticotomy and white matter disruption. Surgeons therefore set aside anterior temporal lobectomy as a last resource and avoid it in the dominant hemisphere. The authors propose a minimally invasive endoscopic intraventricular approach to the temporal horn and describe a standardized analysis and technical assessment of the feasibility of this approach. Methods To determine the best trajectory, angulation, and entry point to the temporal horn of the Lateral Ventricle, the authors evaluated 50 cranial MRI studies (100 temporal lobes) from healthy patients. They studied and systematized the neurosurgical endoscopic anatomy. They also simulated the proposed approach in 9 cadaveric specimens (18 approaches). Results Mean scalp entry point coordinates (± SD) were 2.7 ± 0.28 cm Lateral to the inion and 5.6 ± 0.41 cm superior to...
Andrea Cavaggioni - One of the best experts on this subject based on the ideXlab platform.
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outcome of bovine herpesvirus 4 infection following direct viral injection in the Lateral Ventricle of the mouse brain
Microbes and Infection, 2006Co-Authors: Gaetano Donofrio, C. F. Flammini, Sandro Cavirani, Andrea Cavaggioni, Michela Bondi, Carla MucignatcarettaAbstract:Abstract A recombinant bovine herpesvirus 4 (BoHV-4EGFPΔTK), obtained by the insertion of an EGFP gene into the TK locus of DN 599 BoHV-4 strain, was injected into the Lateral Ventricle of the brain of mice and a clinical score was evaluated for 90 days. Although BoHV-4 was not neuro-pathogenic, BoHV-4EGFPΔTK transduction capability was analyzed. EGFP expression was localized in close proximity to the border of the Ventricles and EGFP-positive cells were found to co-localize with ependymal cells. Although most of the cells had a polarized morphology, they were not neurons. EGFP-positive cells were seen to spread in tangentially oriented rows within the rostral migratory stream (RMS). Co-localization of EGFP signal with anti-GFAP antibody showed that they were glial cells. EGFP-positive cells were observed until 31 days post-injection and then disappeared completely. Virus isolation was possible at an early post-injection time (3 days), but then virus titer was below the detection limits at later times. Viral DNA, however, could be detected until 21 days post-injection. Thus, in this report we showed that (i) BoHV-4EGFPΔTK did not replicate in the mouse brain, (ii) is not pathogenic and (iii) gene transfer can be obtained in long-lived cells belonging to the RMS after BoHV-4EGFPΔTK injection within the Lateral Ventricle.
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outcome of bovine herpesvirus 4 infection following direct viral injection in the Lateral Ventricle of the mouse brain
Microbes and Infection, 2006Co-Authors: Gaetano Donofrio, C. F. Flammini, Sandro Cavirani, Andrea Cavaggioni, Michela Bondi, Carla MucignatcarettaAbstract:A recombinant bovine herpesvirus 4 (BoHV-4EGFPDeltaTK), obtained by the insertion of an EGFP gene into the TK locus of DN 599 BoHV-4 strain, was injected into the Lateral Ventricle of the brain of mice and a clinical score was evaluated for 90 days. Although BoHV-4 was not neuro-pathogenic, BoHV-4EGFPDeltaTK transduction capability was analyzed. EGFP expression was localized in close proximity to the border of the Ventricles and EGFP-positive cells were found to co-localize with ependymal cells. Although most of the cells had a polarized morphology, they were not neurons. EGFP-positive cells were seen to spread in tangentially oriented rows within the rostral migratory stream (RMS). Co-localization of EGFP signal with anti-GFAP antibody showed that they were glial cells. EGFP-positive cells were observed until 31 days post-injection and then disappeared completely. Virus isolation was possible at an early post-injection time (3 days), but then virus titer was below the detection limits at later times. Viral DNA, however, could be detected until 21 days post-injection. Thus, in this report we showed that (i) BoHV-4EGFPDeltaTK did not replicate in the mouse brain, (ii) is not pathogenic and (iii) gene transfer can be obtained in long-lived cells belonging to the RMS after BoHV-4EGFPDeltaTK injection within the Lateral Ventricle.
Sandro Cavirani - One of the best experts on this subject based on the ideXlab platform.
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outcome of bovine herpesvirus 4 infection following direct viral injection in the Lateral Ventricle of the mouse brain
Microbes and Infection, 2006Co-Authors: Gaetano Donofrio, C. F. Flammini, Sandro Cavirani, Andrea Cavaggioni, Michela Bondi, Carla MucignatcarettaAbstract:Abstract A recombinant bovine herpesvirus 4 (BoHV-4EGFPΔTK), obtained by the insertion of an EGFP gene into the TK locus of DN 599 BoHV-4 strain, was injected into the Lateral Ventricle of the brain of mice and a clinical score was evaluated for 90 days. Although BoHV-4 was not neuro-pathogenic, BoHV-4EGFPΔTK transduction capability was analyzed. EGFP expression was localized in close proximity to the border of the Ventricles and EGFP-positive cells were found to co-localize with ependymal cells. Although most of the cells had a polarized morphology, they were not neurons. EGFP-positive cells were seen to spread in tangentially oriented rows within the rostral migratory stream (RMS). Co-localization of EGFP signal with anti-GFAP antibody showed that they were glial cells. EGFP-positive cells were observed until 31 days post-injection and then disappeared completely. Virus isolation was possible at an early post-injection time (3 days), but then virus titer was below the detection limits at later times. Viral DNA, however, could be detected until 21 days post-injection. Thus, in this report we showed that (i) BoHV-4EGFPΔTK did not replicate in the mouse brain, (ii) is not pathogenic and (iii) gene transfer can be obtained in long-lived cells belonging to the RMS after BoHV-4EGFPΔTK injection within the Lateral Ventricle.
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outcome of bovine herpesvirus 4 infection following direct viral injection in the Lateral Ventricle of the mouse brain
Microbes and Infection, 2006Co-Authors: Gaetano Donofrio, C. F. Flammini, Sandro Cavirani, Andrea Cavaggioni, Michela Bondi, Carla MucignatcarettaAbstract:A recombinant bovine herpesvirus 4 (BoHV-4EGFPDeltaTK), obtained by the insertion of an EGFP gene into the TK locus of DN 599 BoHV-4 strain, was injected into the Lateral Ventricle of the brain of mice and a clinical score was evaluated for 90 days. Although BoHV-4 was not neuro-pathogenic, BoHV-4EGFPDeltaTK transduction capability was analyzed. EGFP expression was localized in close proximity to the border of the Ventricles and EGFP-positive cells were found to co-localize with ependymal cells. Although most of the cells had a polarized morphology, they were not neurons. EGFP-positive cells were seen to spread in tangentially oriented rows within the rostral migratory stream (RMS). Co-localization of EGFP signal with anti-GFAP antibody showed that they were glial cells. EGFP-positive cells were observed until 31 days post-injection and then disappeared completely. Virus isolation was possible at an early post-injection time (3 days), but then virus titer was below the detection limits at later times. Viral DNA, however, could be detected until 21 days post-injection. Thus, in this report we showed that (i) BoHV-4EGFPDeltaTK did not replicate in the mouse brain, (ii) is not pathogenic and (iii) gene transfer can be obtained in long-lived cells belonging to the RMS after BoHV-4EGFPDeltaTK injection within the Lateral Ventricle.
