The Experts below are selected from a list of 1059 Experts worldwide ranked by ideXlab platform
Rosario Martín - One of the best experts on this subject based on the ideXlab platform.
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PCR-based methodology for the authentication of grouper (Epinephelus marginatus) in commercial fish fillets
Food Control, 2009Co-Authors: Luis Asensio, Isabel González, María Rojas, Teresa García, Rosario MartínAbstract:In an attempt to authenticate commercial frauds in fish fillets of grouper (Epinephelus marginatus) by being substituted with those of Nile perch (Lates Niloticus) and wreck fish (Polyprion americanus), a polymerase chain reaction (PCR) method, based on the design of specific primers of these species was explored here. The oligonucleotides, which were designed from the 12S ribosomal RNA gene, generated PCR fragments of 100, 138 and 169 bp length for grouper, wreck fish and Nile perch respectively. The specificity of the primers was tested against more than 50 different fish species. Moreover, in this work 70 commercial fish fillets samples were analysed by this methodology; 58 out of them confirmed to be incorrectly labelled. The results suggest that this method may be a reliable tool for the detection of grouper adulteration. Also, this technique may help implementation of traceability systems in the seafood industry.
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identification of grouper epinephelus guaza wreck fish polyprion americanus and nile perch Lates Niloticus fillets by polyclonal antibody based enzyme linked immunosorbent assay
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Luis Asensio, Isabel González, Teresa García, Miguel A Rodriguez, Pablo E Hernandez, Belen Mayoral, Ines Lopezcalleja, Rosario MartínAbstract:An indirect enzyme-linked immunosorbent assay (ELISA) has been developed for the species identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates Niloticus) fillets. The assay was performed in two different formats, microtiter pLates and immunostick tubes, and uses polyclonal antibodies raised in rabbits against muscle-soluble proteins of grouper (anti-GSP), wreck fish (anti-WSP), and Nile perch (anti-PSP). The antibodies were made species-specific by blocking them with the heterologous soluble muscle proteins. Immunorecognition of polyclonal antibodies adsorbed to their specific fish samples was made with swine antirabbit immunoglobulins conjugated to the enzyme horseradish peroxidase. Subsequent enzymatic conversion of the substrate allowed unequivocal identification of the species studied.
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application of random amplified polymorphic dna rapd analysis for identification of grouper epinephelus guaza wreck fish polyprion americanus and nile perch Lates Niloticus fillets
Journal of Food Protection, 2002Co-Authors: Luis Asensio, Isabel González, Teresa García, Alicia Fernandez, Miguel A Rodriguez, Esther Lobo, Pablo E Hernandez, Rosario MartínAbstract:A random amplified polymorphic DNA (RAPD) method was developed for the specific identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates Niloticus) fillets. Using two different reaction primers (S1 and L1), RAPD analysis produced clear fingerprints from which the three fish species could be easily identified. This approach is rapid and reliable and offers the potential to detect fraudulent or unintentional mislabeling of these species in routine seafood authentication analysis.
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identification of nile perch Lates Niloticus grouper epinephelus guaza and wreck fish polyprion americanus fillets by pcr amplification of the 5s rdna gene
Journal of AOAC International, 2001Co-Authors: Luis Asensio, Isabel González, Teresa García, Alicia Fernandez, Miguel A Rodriguez, Pablo E Hernandez, Ana Cespedes, Rosario MartínAbstract:Nile perch (Lates Niloticus), grouper (Epinephelus guaza), and wreck fish (Polyprion americanus) were differentiated by polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene. The design of 3 species-specific primers complementary to the nontranscribed intergenic spacer region from the 5S rDNA molecule allowed amplification of clearly distinguishable gene fragments in each fish species. This approach is rapid and reliable and offers the potential to detect fraudulent or unintentional mislabeling of these species in routine seafood authentication analysis.
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pcr sscp a simple method for the authentication of grouper epinephelus guaza wreck fish polyprion americanus and nile perch Lates Niloticus fillets
Journal of Agricultural and Food Chemistry, 2001Co-Authors: Luis Asensio, Isabel González, Teresa García, Alicia Fernandez, Miguel A Rodriguez, Pablo E Hernandez, Rosario MartínAbstract:A method of DNA analysis has been developed to verify the authenticity of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates Niloticus) fillets. A short fragment (208 bp) of the mitochondrial 12S rRNA gene was amplified by the polymerase chain reaction and analyzed by single-strand conformation polymorphism to get species-specific patterns of single-stranded DNA (ssDNA). DNA strands were separated by native polyacrylamide gel electrophoresis and visualized by silver staining. Discrimination among the three fish species studied was possible, because each one expressed a specific ssDNA pattern.
J H Muyonga - One of the best experts on this subject based on the ideXlab platform.
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extraction and physico chemical characterisation of nile perch Lates Niloticus skin and bone gelatin
Food Hydrocolloids, 2004Co-Authors: J H Muyonga, C G B Cole, Kwaku G DuoduAbstract:Type A gelatins were extracted from skins and bones of young and adult Nile perch and analysed to determine their functional and chemical properties. Total gelatin yield (for sequential extraction at 50, 60, 70 and 95 8C) was in the order adult fish skins . young fish skins . adult fish bones . young fish bones, while percentage gelatin recovery at 50 8C was in the order young fish skins . adult fish skins . young fish bones . adult fish bones. The gelatins obtained were free of fishy odour. Nile perch skin gelatin had turbidity of 20.5 ‐ 158 NTU and ash content of 0.5 ‐ 1.7% while bone gelatins had turbidity of 109 ‐517 NTU and ash content of 4.4‐ 11.2%. Bloom gel strength was 81 ‐ 229 and 134‐ 179 g, respectively, for skin and bone gelatins. Gelatin from adult Nile perch skins exhibited higher viscosity and lower setting time than bone and the young fish skin gelatins. Skin gelatins were found to exhibit higher film tensile strength but lower film percent elongation than bone gelatins. Bone and skin gelatins had similar amino acid composition, with a total imino acid content of about 21.5%. Nile perch skin gelatins had a higher content of polypeptides larger than b compared to bone gelatins. Both bone and skin gelatins also contained low molecular weight ð, aÞ peptides. The differences in functional properties between the skin and bone gelatins appeared to be related to differences in molecular weight distribution of the gelatins. q 2003 Elsevier Ltd. All rights reserved.
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extraction and physico chemical characterisation of nile perch Lates Niloticus skin and bone gelatin
Food Hydrocolloids, 2004Co-Authors: J H Muyonga, C G B Cole, Kwaku G DuoduAbstract:Abstract Type A gelatins were extracted from skins and bones of young and adult Nile perch and analysed to determine their functional and chemical properties. Total gelatin yield (for sequential extraction at 50, 60, 70 and 95 °C) was in the order adult fish skins>young fish skins>adult fish bones>young fish bones, while percentage gelatin recovery at 50 °C was in the order young fish skins>adult fish skins>young fish bones>adult fish bones. The gelatins obtained were free of fishy odour. Nile perch skin gelatin had turbidity of 20.5–158 NTU and ash content of 0.5–1.7% while bone gelatins had turbidity of 109–517 NTU and ash content of 4.4–11.2%. Bloom gel strength was 81–229 and 134–179 g, respectively, for skin and bone gelatins. Gelatin from adult Nile perch skins exhibited higher viscosity and lower setting time than bone and the young fish skin gelatins. Skin gelatins were found to exhibit higher film tensile strength but lower film percent elongation than bone gelatins. Bone and skin gelatins had similar amino acid composition, with a total imino acid content of about 21.5%. Nile perch skin gelatins had a higher content of polypeptides larger than β compared to bone gelatins. Both bone and skin gelatins also contained low molecular weight (
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fourier transform infrared ftir spectroscopic study of acid soluble collagen and gelatin from skins and bones of young and adult nile perch Lates Niloticus
Food Chemistry, 2004Co-Authors: J H Muyonga, C G B Cole, Kwaku G DuoduAbstract:Abstract Fourier transform infrared (FTIR) spectroscopy was conducted on type A gelatins derived from skins and bones of young and adult Nile perch (Lates Niloticus) by a sequential extraction process. Spectra for gelatins were compared to each other and to that of acid soluble collagen from young Nile perch skins, in order to elucidate changes in protein secondary structure during collagen to gelatin transformation. The first gelatin extracts showed diminished amide III bands while the last gelatin extracts showed distinct amide III bands and their amide I bands consisted of a higher percent area of a component around 1690 cm−1. The differences suggested that the collagen to gelatin transition leads to loss of molecular order. The later gelatin extracts exhibited higher molecular order than earlier gelatin extracts, probably because the former contained surviving crosslinks or/and because renaturation of the low molecular weight gelatin fractions (later gelatin extracts) led to formation of more protein–protein linkages.
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characterisation of acid soluble collagen from skins of young and adult nile perch Lates Niloticus
Food Chemistry, 2004Co-Authors: J H Muyonga, C G B Cole, Kwaku G DuoduAbstract:Abstract Acid soluble collagen (ASC) was extracted from the skins of young and adult Nile perch (Lates Niloticus) using 0.5 M acetic acid and precipitation with 0.9 M NaCl. The ASC yields, on a dry weight basis, were 63.1 and 58.7%, respectively for young and adult fish skins. SDS-PAGE showed that the collagens contained two alpha components (α1 and α2). ASC from Nile perch was found to contain more imino acids (19.3 and 20.0%, respectively, for young and adult fish) than most fish species. The denaturation temperature for the collagens from the skins of young and adult Nile perch was determined to be 36 °C, which is also higher than that for most other fish species. Fourier transform infrared spectroscopy showed a higher degree of molecular order in ASC from adult than from young Nile perch. The results indicate that age-related changes in Nile perch skin collagen are not very pronounced, probably because there is minimal development of mature cross-links.
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characterisation of acid soluble collagen from skins of young and adult nile perch Lates Niloticus
Food Chemistry, 2004Co-Authors: J H Muyonga, C G B Cole, Kwaku G DuoduAbstract:Acid soluble collagen (ASC) was extracted from the skins of young and adult Nile perch (Lates Niloticus) using 0.5 M acetic acid and precipitation with 0.9 M NaCl. The ASC yields, on a dry weight basis, were 63.1 and 58.7%, respectively for young and adult fish skins. SDS-PAGE showed that the collagens contained two alpha components (a1 and a2). ASC from Nile perch was found to contain more imino acids (19.3 and 20.0%, respectively, for young and adult fish) than most fish species. The denaturation temperature for the collagens from the skins of young and adult Nile perch was determined to be 36 � C, which is also higher than that for most other fish species. Fourier transform infrared spectroscopy showed a higher degree of molecular order in ASC from adult than from young Nile perch. The results indicate that age-related changes in Nile perch skin collagen are not very pronounced, probably because there is minimal development of mature cross-links. # 2003 Elsevier Ltd. All rights reserved.
Luis Asensio - One of the best experts on this subject based on the ideXlab platform.
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PCR-based methodology for the authentication of grouper (Epinephelus marginatus) in commercial fish fillets
Food Control, 2009Co-Authors: Luis Asensio, Isabel González, María Rojas, Teresa García, Rosario MartínAbstract:In an attempt to authenticate commercial frauds in fish fillets of grouper (Epinephelus marginatus) by being substituted with those of Nile perch (Lates Niloticus) and wreck fish (Polyprion americanus), a polymerase chain reaction (PCR) method, based on the design of specific primers of these species was explored here. The oligonucleotides, which were designed from the 12S ribosomal RNA gene, generated PCR fragments of 100, 138 and 169 bp length for grouper, wreck fish and Nile perch respectively. The specificity of the primers was tested against more than 50 different fish species. Moreover, in this work 70 commercial fish fillets samples were analysed by this methodology; 58 out of them confirmed to be incorrectly labelled. The results suggest that this method may be a reliable tool for the detection of grouper adulteration. Also, this technique may help implementation of traceability systems in the seafood industry.
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identification of grouper epinephelus guaza wreck fish polyprion americanus and nile perch Lates Niloticus fillets by polyclonal antibody based enzyme linked immunosorbent assay
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Luis Asensio, Isabel González, Teresa García, Miguel A Rodriguez, Pablo E Hernandez, Belen Mayoral, Ines Lopezcalleja, Rosario MartínAbstract:An indirect enzyme-linked immunosorbent assay (ELISA) has been developed for the species identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates Niloticus) fillets. The assay was performed in two different formats, microtiter pLates and immunostick tubes, and uses polyclonal antibodies raised in rabbits against muscle-soluble proteins of grouper (anti-GSP), wreck fish (anti-WSP), and Nile perch (anti-PSP). The antibodies were made species-specific by blocking them with the heterologous soluble muscle proteins. Immunorecognition of polyclonal antibodies adsorbed to their specific fish samples was made with swine antirabbit immunoglobulins conjugated to the enzyme horseradish peroxidase. Subsequent enzymatic conversion of the substrate allowed unequivocal identification of the species studied.
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application of random amplified polymorphic dna rapd analysis for identification of grouper epinephelus guaza wreck fish polyprion americanus and nile perch Lates Niloticus fillets
Journal of Food Protection, 2002Co-Authors: Luis Asensio, Isabel González, Teresa García, Alicia Fernandez, Miguel A Rodriguez, Esther Lobo, Pablo E Hernandez, Rosario MartínAbstract:A random amplified polymorphic DNA (RAPD) method was developed for the specific identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates Niloticus) fillets. Using two different reaction primers (S1 and L1), RAPD analysis produced clear fingerprints from which the three fish species could be easily identified. This approach is rapid and reliable and offers the potential to detect fraudulent or unintentional mislabeling of these species in routine seafood authentication analysis.
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identification of nile perch Lates Niloticus grouper epinephelus guaza and wreck fish polyprion americanus fillets by pcr amplification of the 5s rdna gene
Journal of AOAC International, 2001Co-Authors: Luis Asensio, Isabel González, Teresa García, Alicia Fernandez, Miguel A Rodriguez, Pablo E Hernandez, Ana Cespedes, Rosario MartínAbstract:Nile perch (Lates Niloticus), grouper (Epinephelus guaza), and wreck fish (Polyprion americanus) were differentiated by polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene. The design of 3 species-specific primers complementary to the nontranscribed intergenic spacer region from the 5S rDNA molecule allowed amplification of clearly distinguishable gene fragments in each fish species. This approach is rapid and reliable and offers the potential to detect fraudulent or unintentional mislabeling of these species in routine seafood authentication analysis.
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pcr sscp a simple method for the authentication of grouper epinephelus guaza wreck fish polyprion americanus and nile perch Lates Niloticus fillets
Journal of Agricultural and Food Chemistry, 2001Co-Authors: Luis Asensio, Isabel González, Teresa García, Alicia Fernandez, Miguel A Rodriguez, Pablo E Hernandez, Rosario MartínAbstract:A method of DNA analysis has been developed to verify the authenticity of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates Niloticus) fillets. A short fragment (208 bp) of the mitochondrial 12S rRNA gene was amplified by the polymerase chain reaction and analyzed by single-strand conformation polymorphism to get species-specific patterns of single-stranded DNA (ssDNA). DNA strands were separated by native polyacrylamide gel electrophoresis and visualized by silver staining. Discrimination among the three fish species studied was possible, because each one expressed a specific ssDNA pattern.
Kwaku G Duodu - One of the best experts on this subject based on the ideXlab platform.
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extraction and physico chemical characterisation of nile perch Lates Niloticus skin and bone gelatin
Food Hydrocolloids, 2004Co-Authors: J H Muyonga, C G B Cole, Kwaku G DuoduAbstract:Type A gelatins were extracted from skins and bones of young and adult Nile perch and analysed to determine their functional and chemical properties. Total gelatin yield (for sequential extraction at 50, 60, 70 and 95 8C) was in the order adult fish skins . young fish skins . adult fish bones . young fish bones, while percentage gelatin recovery at 50 8C was in the order young fish skins . adult fish skins . young fish bones . adult fish bones. The gelatins obtained were free of fishy odour. Nile perch skin gelatin had turbidity of 20.5 ‐ 158 NTU and ash content of 0.5 ‐ 1.7% while bone gelatins had turbidity of 109 ‐517 NTU and ash content of 4.4‐ 11.2%. Bloom gel strength was 81 ‐ 229 and 134‐ 179 g, respectively, for skin and bone gelatins. Gelatin from adult Nile perch skins exhibited higher viscosity and lower setting time than bone and the young fish skin gelatins. Skin gelatins were found to exhibit higher film tensile strength but lower film percent elongation than bone gelatins. Bone and skin gelatins had similar amino acid composition, with a total imino acid content of about 21.5%. Nile perch skin gelatins had a higher content of polypeptides larger than b compared to bone gelatins. Both bone and skin gelatins also contained low molecular weight ð, aÞ peptides. The differences in functional properties between the skin and bone gelatins appeared to be related to differences in molecular weight distribution of the gelatins. q 2003 Elsevier Ltd. All rights reserved.
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extraction and physico chemical characterisation of nile perch Lates Niloticus skin and bone gelatin
Food Hydrocolloids, 2004Co-Authors: J H Muyonga, C G B Cole, Kwaku G DuoduAbstract:Abstract Type A gelatins were extracted from skins and bones of young and adult Nile perch and analysed to determine their functional and chemical properties. Total gelatin yield (for sequential extraction at 50, 60, 70 and 95 °C) was in the order adult fish skins>young fish skins>adult fish bones>young fish bones, while percentage gelatin recovery at 50 °C was in the order young fish skins>adult fish skins>young fish bones>adult fish bones. The gelatins obtained were free of fishy odour. Nile perch skin gelatin had turbidity of 20.5–158 NTU and ash content of 0.5–1.7% while bone gelatins had turbidity of 109–517 NTU and ash content of 4.4–11.2%. Bloom gel strength was 81–229 and 134–179 g, respectively, for skin and bone gelatins. Gelatin from adult Nile perch skins exhibited higher viscosity and lower setting time than bone and the young fish skin gelatins. Skin gelatins were found to exhibit higher film tensile strength but lower film percent elongation than bone gelatins. Bone and skin gelatins had similar amino acid composition, with a total imino acid content of about 21.5%. Nile perch skin gelatins had a higher content of polypeptides larger than β compared to bone gelatins. Both bone and skin gelatins also contained low molecular weight (
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fourier transform infrared ftir spectroscopic study of acid soluble collagen and gelatin from skins and bones of young and adult nile perch Lates Niloticus
Food Chemistry, 2004Co-Authors: J H Muyonga, C G B Cole, Kwaku G DuoduAbstract:Abstract Fourier transform infrared (FTIR) spectroscopy was conducted on type A gelatins derived from skins and bones of young and adult Nile perch (Lates Niloticus) by a sequential extraction process. Spectra for gelatins were compared to each other and to that of acid soluble collagen from young Nile perch skins, in order to elucidate changes in protein secondary structure during collagen to gelatin transformation. The first gelatin extracts showed diminished amide III bands while the last gelatin extracts showed distinct amide III bands and their amide I bands consisted of a higher percent area of a component around 1690 cm−1. The differences suggested that the collagen to gelatin transition leads to loss of molecular order. The later gelatin extracts exhibited higher molecular order than earlier gelatin extracts, probably because the former contained surviving crosslinks or/and because renaturation of the low molecular weight gelatin fractions (later gelatin extracts) led to formation of more protein–protein linkages.
-
characterisation of acid soluble collagen from skins of young and adult nile perch Lates Niloticus
Food Chemistry, 2004Co-Authors: J H Muyonga, C G B Cole, Kwaku G DuoduAbstract:Abstract Acid soluble collagen (ASC) was extracted from the skins of young and adult Nile perch (Lates Niloticus) using 0.5 M acetic acid and precipitation with 0.9 M NaCl. The ASC yields, on a dry weight basis, were 63.1 and 58.7%, respectively for young and adult fish skins. SDS-PAGE showed that the collagens contained two alpha components (α1 and α2). ASC from Nile perch was found to contain more imino acids (19.3 and 20.0%, respectively, for young and adult fish) than most fish species. The denaturation temperature for the collagens from the skins of young and adult Nile perch was determined to be 36 °C, which is also higher than that for most other fish species. Fourier transform infrared spectroscopy showed a higher degree of molecular order in ASC from adult than from young Nile perch. The results indicate that age-related changes in Nile perch skin collagen are not very pronounced, probably because there is minimal development of mature cross-links.
-
characterisation of acid soluble collagen from skins of young and adult nile perch Lates Niloticus
Food Chemistry, 2004Co-Authors: J H Muyonga, C G B Cole, Kwaku G DuoduAbstract:Acid soluble collagen (ASC) was extracted from the skins of young and adult Nile perch (Lates Niloticus) using 0.5 M acetic acid and precipitation with 0.9 M NaCl. The ASC yields, on a dry weight basis, were 63.1 and 58.7%, respectively for young and adult fish skins. SDS-PAGE showed that the collagens contained two alpha components (a1 and a2). ASC from Nile perch was found to contain more imino acids (19.3 and 20.0%, respectively, for young and adult fish) than most fish species. The denaturation temperature for the collagens from the skins of young and adult Nile perch was determined to be 36 � C, which is also higher than that for most other fish species. Fourier transform infrared spectroscopy showed a higher degree of molecular order in ASC from adult than from young Nile perch. The results indicate that age-related changes in Nile perch skin collagen are not very pronounced, probably because there is minimal development of mature cross-links. # 2003 Elsevier Ltd. All rights reserved.
Isabel González - One of the best experts on this subject based on the ideXlab platform.
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PCR-based methodology for the authentication of grouper (Epinephelus marginatus) in commercial fish fillets
Food Control, 2009Co-Authors: Luis Asensio, Isabel González, María Rojas, Teresa García, Rosario MartínAbstract:In an attempt to authenticate commercial frauds in fish fillets of grouper (Epinephelus marginatus) by being substituted with those of Nile perch (Lates Niloticus) and wreck fish (Polyprion americanus), a polymerase chain reaction (PCR) method, based on the design of specific primers of these species was explored here. The oligonucleotides, which were designed from the 12S ribosomal RNA gene, generated PCR fragments of 100, 138 and 169 bp length for grouper, wreck fish and Nile perch respectively. The specificity of the primers was tested against more than 50 different fish species. Moreover, in this work 70 commercial fish fillets samples were analysed by this methodology; 58 out of them confirmed to be incorrectly labelled. The results suggest that this method may be a reliable tool for the detection of grouper adulteration. Also, this technique may help implementation of traceability systems in the seafood industry.
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identification of grouper epinephelus guaza wreck fish polyprion americanus and nile perch Lates Niloticus fillets by polyclonal antibody based enzyme linked immunosorbent assay
Journal of Agricultural and Food Chemistry, 2003Co-Authors: Luis Asensio, Isabel González, Teresa García, Miguel A Rodriguez, Pablo E Hernandez, Belen Mayoral, Ines Lopezcalleja, Rosario MartínAbstract:An indirect enzyme-linked immunosorbent assay (ELISA) has been developed for the species identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates Niloticus) fillets. The assay was performed in two different formats, microtiter pLates and immunostick tubes, and uses polyclonal antibodies raised in rabbits against muscle-soluble proteins of grouper (anti-GSP), wreck fish (anti-WSP), and Nile perch (anti-PSP). The antibodies were made species-specific by blocking them with the heterologous soluble muscle proteins. Immunorecognition of polyclonal antibodies adsorbed to their specific fish samples was made with swine antirabbit immunoglobulins conjugated to the enzyme horseradish peroxidase. Subsequent enzymatic conversion of the substrate allowed unequivocal identification of the species studied.
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application of random amplified polymorphic dna rapd analysis for identification of grouper epinephelus guaza wreck fish polyprion americanus and nile perch Lates Niloticus fillets
Journal of Food Protection, 2002Co-Authors: Luis Asensio, Isabel González, Teresa García, Alicia Fernandez, Miguel A Rodriguez, Esther Lobo, Pablo E Hernandez, Rosario MartínAbstract:A random amplified polymorphic DNA (RAPD) method was developed for the specific identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates Niloticus) fillets. Using two different reaction primers (S1 and L1), RAPD analysis produced clear fingerprints from which the three fish species could be easily identified. This approach is rapid and reliable and offers the potential to detect fraudulent or unintentional mislabeling of these species in routine seafood authentication analysis.
-
identification of nile perch Lates Niloticus grouper epinephelus guaza and wreck fish polyprion americanus fillets by pcr amplification of the 5s rdna gene
Journal of AOAC International, 2001Co-Authors: Luis Asensio, Isabel González, Teresa García, Alicia Fernandez, Miguel A Rodriguez, Pablo E Hernandez, Ana Cespedes, Rosario MartínAbstract:Nile perch (Lates Niloticus), grouper (Epinephelus guaza), and wreck fish (Polyprion americanus) were differentiated by polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene. The design of 3 species-specific primers complementary to the nontranscribed intergenic spacer region from the 5S rDNA molecule allowed amplification of clearly distinguishable gene fragments in each fish species. This approach is rapid and reliable and offers the potential to detect fraudulent or unintentional mislabeling of these species in routine seafood authentication analysis.
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pcr sscp a simple method for the authentication of grouper epinephelus guaza wreck fish polyprion americanus and nile perch Lates Niloticus fillets
Journal of Agricultural and Food Chemistry, 2001Co-Authors: Luis Asensio, Isabel González, Teresa García, Alicia Fernandez, Miguel A Rodriguez, Pablo E Hernandez, Rosario MartínAbstract:A method of DNA analysis has been developed to verify the authenticity of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates Niloticus) fillets. A short fragment (208 bp) of the mitochondrial 12S rRNA gene was amplified by the polymerase chain reaction and analyzed by single-strand conformation polymorphism to get species-specific patterns of single-stranded DNA (ssDNA). DNA strands were separated by native polyacrylamide gel electrophoresis and visualized by silver staining. Discrimination among the three fish species studied was possible, because each one expressed a specific ssDNA pattern.
