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Johan Buyse - One of the best experts on this subject based on the ideXlab platform.
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dynamic changes in parameters of redox balance after mild heat stress in aged Laying Hens gallus gallus domesticus
Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2008Co-Authors: Eddy Decuypere, Johan BuyseAbstract:Abstract In order to evaluate the metabolic responses of Laying Hens induced by high temperature at later Laying stage, nine 60-wk-old Laying Hens ( Gallus gallus domesticus ) were employed in the present study. The Hens were exposed to 32 °C for 21 d and blood samples were obtained before and at 1, 7, 14 and 21 d of heat exposure. The reactive oxygen species (ROS) formed in blood during heat exposure were estimated by the ex vivo spin-trapping method. Body temperature and plasma concentrations of glucose, urate, creatine kinase (CK), triiodothyronine (T 3 ), thyroxine (T 4 ), corticosterone (CORT), thiobarbituric acid reacting substances (TBARS), ferric/reducing antioxidant power (FRAP) and superoxide dismutase (SOD) activity were measured. Plasma levels of glucose, CK and CORT were not significantly influenced by heat exposure at any time point. The circulating concentrations of T 3 were decreased while plasma T 4 levels changed in the opposite way. The formation of ROS was significantly augmented by heat exposure in Laying Hens though the body temperature was not significantly altered. The enhanced enzymatic and non-enzymatic antioxidant systems acted in concert to alleviate the heat stress evoked oxidative damage.
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dynamic changes in parameters of redox balance after mild heat stress in aged Laying Hens gallus gallus domesticus
Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2008Co-Authors: Han Chung Lin, Eddy Decuypere, Dirk De Vos, Johan BuyseAbstract:In order to evaluate the metabolic responses of Laying Hens induced by high temperature at later Laying stage, nine 60-wk-old Laying Hens (Gallus gallus domesticus) were employed in the present study. The Hens were exposed to 32 degrees C for 21 d and blood samples were obtained before and at 1, 7, 14 and 21 d of heat exposure. The reactive oxygen species (ROS) formed in blood during heat exposure were estimated by the ex vivo spin-trapping method. Body temperature and plasma concentrations of glucose, urate, creatine kinase (CK), triiodothyronine (T(3)), thyroxine (T(4)), corticosterone (CORT), thiobarbituric acid reacting substances (TBARS), ferric/reducing antioxidant power (FRAP) and superoxide dismutase (SOD) activity were measured. Plasma levels of glucose, CK and CORT were not significantly influenced by heat exposure at any time point. The circulating concentrations of T(3) were decreased while plasma T(4) levels changed in the opposite way. The formation of ROS was significantly augmented by heat exposure in Laying Hens though the body temperature was not significantly altered. The enhanced enzymatic and non-enzymatic antioxidant systems acted in concert to alleviate the heat stress evoked oxidative damage.
Eddy Decuypere - One of the best experts on this subject based on the ideXlab platform.
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dynamic changes in parameters of redox balance after mild heat stress in aged Laying Hens gallus gallus domesticus
Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2008Co-Authors: Eddy Decuypere, Johan BuyseAbstract:Abstract In order to evaluate the metabolic responses of Laying Hens induced by high temperature at later Laying stage, nine 60-wk-old Laying Hens ( Gallus gallus domesticus ) were employed in the present study. The Hens were exposed to 32 °C for 21 d and blood samples were obtained before and at 1, 7, 14 and 21 d of heat exposure. The reactive oxygen species (ROS) formed in blood during heat exposure were estimated by the ex vivo spin-trapping method. Body temperature and plasma concentrations of glucose, urate, creatine kinase (CK), triiodothyronine (T 3 ), thyroxine (T 4 ), corticosterone (CORT), thiobarbituric acid reacting substances (TBARS), ferric/reducing antioxidant power (FRAP) and superoxide dismutase (SOD) activity were measured. Plasma levels of glucose, CK and CORT were not significantly influenced by heat exposure at any time point. The circulating concentrations of T 3 were decreased while plasma T 4 levels changed in the opposite way. The formation of ROS was significantly augmented by heat exposure in Laying Hens though the body temperature was not significantly altered. The enhanced enzymatic and non-enzymatic antioxidant systems acted in concert to alleviate the heat stress evoked oxidative damage.
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dynamic changes in parameters of redox balance after mild heat stress in aged Laying Hens gallus gallus domesticus
Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2008Co-Authors: Han Chung Lin, Eddy Decuypere, Dirk De Vos, Johan BuyseAbstract:In order to evaluate the metabolic responses of Laying Hens induced by high temperature at later Laying stage, nine 60-wk-old Laying Hens (Gallus gallus domesticus) were employed in the present study. The Hens were exposed to 32 degrees C for 21 d and blood samples were obtained before and at 1, 7, 14 and 21 d of heat exposure. The reactive oxygen species (ROS) formed in blood during heat exposure were estimated by the ex vivo spin-trapping method. Body temperature and plasma concentrations of glucose, urate, creatine kinase (CK), triiodothyronine (T(3)), thyroxine (T(4)), corticosterone (CORT), thiobarbituric acid reacting substances (TBARS), ferric/reducing antioxidant power (FRAP) and superoxide dismutase (SOD) activity were measured. Plasma levels of glucose, CK and CORT were not significantly influenced by heat exposure at any time point. The circulating concentrations of T(3) were decreased while plasma T(4) levels changed in the opposite way. The formation of ROS was significantly augmented by heat exposure in Laying Hens though the body temperature was not significantly altered. The enhanced enzymatic and non-enzymatic antioxidant systems acted in concert to alleviate the heat stress evoked oxidative damage.
Han Chung Lin - One of the best experts on this subject based on the ideXlab platform.
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dynamic changes in parameters of redox balance after mild heat stress in aged Laying Hens gallus gallus domesticus
Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2008Co-Authors: Han Chung Lin, Eddy Decuypere, Dirk De Vos, Johan BuyseAbstract:In order to evaluate the metabolic responses of Laying Hens induced by high temperature at later Laying stage, nine 60-wk-old Laying Hens (Gallus gallus domesticus) were employed in the present study. The Hens were exposed to 32 degrees C for 21 d and blood samples were obtained before and at 1, 7, 14 and 21 d of heat exposure. The reactive oxygen species (ROS) formed in blood during heat exposure were estimated by the ex vivo spin-trapping method. Body temperature and plasma concentrations of glucose, urate, creatine kinase (CK), triiodothyronine (T(3)), thyroxine (T(4)), corticosterone (CORT), thiobarbituric acid reacting substances (TBARS), ferric/reducing antioxidant power (FRAP) and superoxide dismutase (SOD) activity were measured. Plasma levels of glucose, CK and CORT were not significantly influenced by heat exposure at any time point. The circulating concentrations of T(3) were decreased while plasma T(4) levels changed in the opposite way. The formation of ROS was significantly augmented by heat exposure in Laying Hens though the body temperature was not significantly altered. The enhanced enzymatic and non-enzymatic antioxidant systems acted in concert to alleviate the heat stress evoked oxidative damage.
T J Applegate - One of the best experts on this subject based on the ideXlab platform.
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standardized ileal amino acid digestibility of corn corn distillers dried grains with solubles wheat middlings and bakery by products in broilers and Laying Hens
Poultry Science, 2015Co-Authors: S A Adedokun, P Jaynes, R L Payne, T J ApplegateAbstract:: Standardized ileal amino acid digestibility (SIAAD) of 5 samples of corn distillers dried grain with solubles (DDGS), 5 samples of bakery by-products (BBP), 3 samples of corn, and 1 sample of wheat middlings (WM) were evaluated in broilers and Laying Hens. Diets containing each of the 14 feed ingredients were evaluated in 21 day-old broiler chickens. The DDGS and BBP containing diets were fed to 30-week-old Laying Hens, while corn and wheat middling were evaluated in 50-week-old Laying Hens. All the diets were semi-purified with each feed ingredient being the only source of amino acid (AA). To obtain SIAAD values, apparent ileal AA digestibility was corrected for basal ileal endogenous AA losses using values generated from broilers and Laying Hens fed a nitrogen-free diet. Ileal crude protein digestibility for the 5 DDGS samples was higher (P < 0.05) in broilers than in Laying Hens. Broilers had higher SIAAD for DDGS 2, 3, 4, and 5 while there was no difference for DDGS 1 except for 4 AA where broilers had higher (P < 0.05) SIAAD values. Standardized ileal AA digestibility values for broilers were higher (P < 0.05) for BBP 1 and 4. Ileal CP digestibility for corn 1 was higher (P < 0.05) for broilers compared to Laying Hens, and SIAAD values for the 16 AA (9 indispensable and 7 dispensable) evaluated in this study were higher (P < 0.05) in broilers. Broilers had higher (P < 0.05) SIAAD values for 4 (histidine, leucine, phenylalanine, and valine) and 6 (histidine, leucine, methionine, phenylalanine, threonine, and valine) indispensable and 3 (cysteine, glutamic acid, and proline) and 4 (cysteine, glutamic acid, proline, and serine) dispensable AA for corn 2 and corn 3, respectively. No difference in SIAAD between broilers and Laying Hens was observed for WM. Results from this study confirm that high variability in digestibility exists between different samples of DDGS. Differences in SIAAD between broilers and Laying Hens were observed in some samples of DDGS and BBP.
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standardized ileal amino acid digestibility of meat and bone meal and soybean meal in Laying Hens and broilers
Poultry Science, 2014Co-Authors: S A Adedokun, P Jaynes, R L Payne, M Abd E Elhack, T J ApplegateAbstract:The objective of this study was to determine the standardized ileal amino acid digestibility (SIAAD) of 7 meat and bone meal (MBM) and 3 soybean meal (SBM) samples in broilers (Ross 708) and Laying Hens (Hy-line W36). All 10 feed ingredients were evaluated in 21-d-old broiler chickens and 30- or 50-wk-old Laying Hens. Standardization was accomplished by correcting for basal ileal endogenous amino acid losses using a nitrogen-free diet. Broilers were reared in cages from d 0 to 16 on a standard broiler starter diet adequate in all nutrients and energy; thereafter, they were allotted to treatments using a randomized complete design with 6 replicate cages of 8 birds each. For the Laying Hens, 6 replicate cages of 6 birds each (542 cm(2)/bird) were used. Each treatment diet, which was fed for 5 d, was semipurified, with MBM or SBM being the sole source of amino acids in each diet. Ileal endogenous amino acid losses were not different between broilers and the 2 groups of Laying Hens. Meat and bone meal from different locations varied widely in digestibility. Broilers had higher (P < 0.05) SIAAD in 4 of the 7 MBM samples. In 2 of the 3 SBM samples, broilers had higher (P < 0.05) SIAAD for most of the nonessential amino acids. Generally, Hens had 6.4 and 7.7% units less Met and Lys digestibility of all MBM samples after standardization. Dry matter digestibility values of the SBM samples were higher (P < 0.05) in broilers. Likewise, broilers had 4.1 and 1.5% units more Met and Lys digestibility of all the SBM samples evaluated compared with those from Laying Hens. The results of these experiments suggest that differences exist in the digestive capabilities of Laying Hens and broilers, which indicates that species-specific nutrient digestibility values or adjustments may be needed.
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comparison of endogenous amino acid flow in broilers Laying Hens and caecectomised roosters
British Poultry Science, 2009Co-Authors: S A Adedokun, P L Utterback, C M Parsons, O Adeola, M S Lilburn, T J ApplegateAbstract:1. This study was conducted to determine and compare endogenous amino acid (EAA) flow in broilers, Laying Hens, and caecectomised roosters fed on either a nitrogen-free diet (NFD) or a highly digestible protein diet (HDP). 2. Also, the EAA flows using three methods (fasted, NFD, or HDP) in precision-fed caecectomised roosters were compared. 3. EAA flow did not differ between broilers and Laying Hens on either the NFD or HDP diets. 4. Endogenous amino acid flow in caecectomised roosters was 3·5–12-fold higher than for broilers and Laying Hens for both methods (NFD and HDP) of estimation. 5. When caecectomised roosters were fasted or precision-fed (NFD or HDP), there was no difference in EAA flow between the NFD- or HDP-fed birds. 6. However, flows from fasted roosters were lower than from roosters receiving either the NFD or HDP diet. 7. Results from this study showed that EAA flow is method-dependent. Total amino acid flow from fasted birds was 31% less than when a NFD was fed to caecectomised roosters.
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comparison of amino acid digestibility of feed ingredients in broilers Laying Hens and caecectomised roosters
British Poultry Science, 2009Co-Authors: S A Adedokun, P L Utterback, C M Parsons, O Adeola, M S Lilburn, T J ApplegateAbstract:1. This study determined the effect of bird type (broilers, Laying Hens, or caecectomised roosters) on amino acid digestibility of feedstuffs from 5 plant sources and one animal source. 2. The standardised amino acid digestibility (SAAD) were obtained by correcting apparent ileal amino acid digestibility (AIAAD) values for basal ileal endogenous amino acid (EAA) flow obtained by feeding a N-free diet (NFD) to broilers and Laying Hens or from fasted EAA flow from caecectomised roosters. 3. The apparent total amino acid (TAA) digestibilities did not differ between broilers and roosters for three of the 6 feed ingredients. 4. Broilers had higher apparent total amino acid (TAA) digestibility than Laying Hens and roosters when fed on the maize diet (canola meal, maize, and soybean meal). 5. The apparent TAA digestibilities were similar across bird types for the dark distillers’ dried grain with solubles, but the apparent lysine digestibility was much lower in the caecectomised roosters (15%) than the broilers ...
Dirk De Vos - One of the best experts on this subject based on the ideXlab platform.
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dynamic changes in parameters of redox balance after mild heat stress in aged Laying Hens gallus gallus domesticus
Comparative Biochemistry and Physiology C-toxicology & Pharmacology, 2008Co-Authors: Han Chung Lin, Eddy Decuypere, Dirk De Vos, Johan BuyseAbstract:In order to evaluate the metabolic responses of Laying Hens induced by high temperature at later Laying stage, nine 60-wk-old Laying Hens (Gallus gallus domesticus) were employed in the present study. The Hens were exposed to 32 degrees C for 21 d and blood samples were obtained before and at 1, 7, 14 and 21 d of heat exposure. The reactive oxygen species (ROS) formed in blood during heat exposure were estimated by the ex vivo spin-trapping method. Body temperature and plasma concentrations of glucose, urate, creatine kinase (CK), triiodothyronine (T(3)), thyroxine (T(4)), corticosterone (CORT), thiobarbituric acid reacting substances (TBARS), ferric/reducing antioxidant power (FRAP) and superoxide dismutase (SOD) activity were measured. Plasma levels of glucose, CK and CORT were not significantly influenced by heat exposure at any time point. The circulating concentrations of T(3) were decreased while plasma T(4) levels changed in the opposite way. The formation of ROS was significantly augmented by heat exposure in Laying Hens though the body temperature was not significantly altered. The enhanced enzymatic and non-enzymatic antioxidant systems acted in concert to alleviate the heat stress evoked oxidative damage.
