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Lidija Senerovic - One of the best experts on this subject based on the ideXlab platform.
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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans Leaf Extracts.
Journal of ethnopharmacology, 2018Co-Authors: Ivana Aleksic, Ivana D. Radojević, Petar Ristivojević, Aleksandar Pavic, Ljiljana R. Čomić, Branka Vasiljevic, Dejan Opsenica, Dušanka Milojković-opsenica, Lidija SenerovicAbstract:Abstract Ethnopharmacological relevance Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) Leaf Extracts. Materials and methods The anti-quorum sensing activity of Tn Extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of Extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans Leafs Extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results Subinhibitory concentrations of Tn Leaf Extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn Extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions This study demonstrates anti-virulence potential of Tn Leaf Extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.
Beom Soo Kim - One of the best experts on this subject based on the ideXlab platform.
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Biological synthesis of nanomaterials using plant Leaf Extracts
2013 13th IEEE International Conference on Nanotechnology (IEEE-NANO 2013), 2013Co-Authors: Beom Soo KimAbstract:Several plant Leaf Extracts (Pine, Persimmon, Ginkgo, Magnolia, Platanus, Cherry, etc.) were used and compared for their extracellular synthesis of nanomaterials. Stable gold, silver, copper, and platinum nanoparticles were formed by treating aqueous solution of HAuCl4, AgNO3, CuSO4·5H2O, and H2PtCl6·6H2O, respectively, with the plant Leaf Extracts as reducing agent. The biologically synthesized silver and copper nanoparticles showed antibacterial activities when they were coated on the surface of latex foam products. Graphene oxide, which was prepared by oxidation of natural graphite powders, was also reduced using plant Leaf Extracts. The reduced materials were characterized by elemental analysis, UV-vis spectroscopy, FT-IR, Raman spectroscopy, X-ray diffraction, transmission electron microscopy, thermo-gravimetric analysis, etc.
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biological synthesis of gold nanoparticles using magnolia kobus and diopyros kaki Leaf Extracts
Process Biochemistry, 2009Co-Authors: Jae Yong Song, Hyeonkyeong Jang, Beom Soo KimAbstract:Abstract Leaf Extracts of two plants, Magnolia kobus and Diopyros kaki , were used for ecofriendly extracellular synthesis of metallic gold nanoparticles. Stable gold nanoparticles were formed by treating an aqueous HAuCl 4 solution using the plant Leaf Extracts as reducing agents. UV–visible spectroscopy was used for quantification of gold nanoparticle synthesis. Only a few minutes were required for >90% conversion to gold nanoparticles at a reaction temperature of 95 °C, suggesting reaction rates higher or comparable to those of nanoparticle synthesis by chemical methods. The synthesized gold nanoparticles were characterized with inductively coupled plasma spectrometry (ICP), energy-dispersive X-ray spectroscopy (EDS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), and particle analysis using a particle analyzer. SEM and TEM images showed that a mixture of plate (triangles, pentagons, and hexagons) and spherical structures (size, 5–300 nm) were formed at lower temperatures and Leaf broth concentrations, while smaller spherical shapes were obtained at higher temperatures and Leaf broth concentrations.
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Rapid biological synthesis of silver nanoparticles using plant Leaf Extracts
Bioprocess and Biosystems Engineering, 2009Co-Authors: Jae Yong Song, Beom Soo KimAbstract:Five plant Leaf Extracts (Pine, Persimmon, Ginkgo, Magnolia and Platanus) were used and compared for their extracellular synthesis of metallic silver nanoparticles. Stable silver nanoparticles were formed by treating aqueous solution of AgNO3 with the plant Leaf Extracts as reducing agent of Ag+ to Ag0. UV-visible spectroscopy was used to monitor the quantitative formation of silver nanoparticles. Magnolia Leaf broth was the best reducing agent in terms of synthesis rate and conversion to silver nanoparticles. Only 11 min was required for more than 90% conversion at the reaction temperature of 95 °C using Magnolia Leaf broth. The synthesized silver nanoparticles were characterized with inductively coupled plasma spectrometry (ICP), energy dispersive X-ray spectroscopy (EDS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle analyzer. The average particle size ranged from 15 to 500 nm. The particle size could be controlled by changing the reaction temperature, Leaf broth concentration and AgNO3 concentration. This environmentally friendly method of biological silver nanoparticles production provides rates of synthesis faster or comparable to those of chemical methods and can potentially be used in various human contacting areas such as cosmetics, foods and medical applications.
Šenerović Lidija - One of the best experts on this subject based on the ideXlab platform.
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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans Leaf Extracts
Elsevier Ireland Ltd Clare, 2018Co-Authors: Aleksić Ivana, Ristivojević Petar, Pavić Aleksandar, Radojević Ivana, Čomić, Ljiljana R., Vasiljević Branka, Opsenica, Dejan M., Milojković-opsenica Dušanka, Šenerović LidijaAbstract:Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) Leaf Extracts. Materials and methods: The anti-quorum sensing activity of Tn Extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of Extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans Leafs Extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn Leaf Extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn Extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn Leaf Extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.This is the peer-reviewed version of the following article: Aleksic, I.; Ristivojevic, P.; Pavic, A.; Radojević, I.; Čomić, L. R.; Vasiljevic, B.; Opsenica, D.; Milojković-Opsenica, D.; Senerovic, L. Anti-Quorum Sensing Activity, Toxicity in Zebrafish (Danio Rerio) Embryos and Phytochemical Characterization of Trapa Natans Leaf Extracts. Journal of Ethnopharmacology 2018, 222, 148–158. [https://doi.org/10.1016/j.jep.2018.05.005]Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/2933
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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans Leaf Extracts
'Elsevier BV', 2018Co-Authors: Aleksić Ivana, Ristivojević Petar, Pavić Aleksandar, Radojević Ivana, Čomić, Ljiljana R., Vasiljević Branka, Milojković-opsenica Dušanka, Opsenica Dejan, Šenerović LidijaAbstract:Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) Leaf Extracts. Materials and methods: The anti-quorum sensing activity of Tn Extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of Extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans Leafs Extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn Leaf Extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn Extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn Leaf Extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.This is the peer-reviewed version of the following article: Aleksic, I.; Ristivojevic, P.; Pavic, A.; Radojević, I.; Čomić, L. R.; Vasiljevic, B.; Opsenica, D.; Milojković-Opsenica, D.; Senerovic, L. Anti-Quorum Sensing Activity, Toxicity in Zebrafish (Danio Rerio) Embryos and Phytochemical Characterization of Trapa Natans Leaf Extracts. Journal of Ethnopharmacology 2018, 222, 148–158. [https://doi.org/10.1016/j.jep.2018.05.005][http://cer.ihtm.bg.ac.rs/handle/123456789/2310
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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans Leaf Extracts
Elsevier Ireland Ltd Clare, 2018Co-Authors: Aleksić Ivana, Ristivojević Petar, Pavić Aleksandar, Vasiljević Branka, Milojković-opsenica Dušanka, Opsenica Dejan, Radojevic Ivana, Comic, Ljiljana R., Šenerović LidijaAbstract:Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) Leaf Extracts. Materials and methods: The anti-quorum sensing activity of Tn Extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of Extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans Leafs Extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn Leaf Extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn Extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn Leaf Extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.The peer-reviewed version: [http://cer.ihtm.bg.ac.rs/handle/123456789/3139
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Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans Leaf Extracts
Elsevier Ireland Ltd Clare, 2018Co-Authors: Aleksić Ivana, Ristivojević Petar, Pavić Aleksandar, Radojević Ivana, Čomić, Ljiljana R., Vasiljević Branka, Opsenica, Dejan M., Milojković-opsenica Dušanka, Šenerović LidijaAbstract:Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) Leaf Extracts. Materials and methods: The anti-quorum sensing activity of Tn Extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of Extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans Leafs Extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn Leaf Extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn Extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn Leaf Extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.Peer-reviewed manuscript: [http://cherry.chem.bg.ac.rs/handle/123456789/2932]Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/2933
Wei-xi Tian - One of the best experts on this subject based on the ideXlab platform.
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Inhibitory effects on bacterial growth and beta-ketoacyl-ACP reductase by different species of maple Leaf Extracts and tannic acid.
Phytotherapy research : PTR, 2010Co-Authors: Xue-fu You, Wei-xi TianAbstract:It is important to develop new antibiotics aimed at novel targets. The investigation found that the Leaf Extracts from five maples (Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge collected in Denmark, Canada and China) and their component tannic acid displayed antibacterial ability against 24 standard bacteria strains with the minimum inhibitory concentration of 0.3-8.0 mg/mL. Unlike the standard antibiotic levofloxacin (LFX), these samples inhibited Gram-positive bacteria more effectively than they inhibited Gram-negative bacteria. These samples effectively inhibited two antidrug bacterial strains. The results show that these samples inhibit bacteria by a different mechanism from LFX. These samples potently inhibited b-ketoacyl-ACP reductase (FabG), which is an important enzyme in bacterial fatty acid synthesis. Tannic acid showed the strongest inhibition on FabG with a half inhibition concentration of 0.78 microM (0.81 microg/mL). Furthermore, tannic acid and two maple Leaf Extracts showed time-dependent irreversible inhibition of FabG. These three samples also exhibited better inhibition on bacteria. It is suggested that FabG is the antibacteria target of maple Leaf Extracts and tannic acid, and both reversible and irreversible inhibitions of FabG are important for the antibacterial effect.
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Inhibitory effects on bacterial growth and b-ketoacyl-ACP reductase by different species of maple Leaf Extracts and tannic acid
Phytotherapy Research, 2009Co-Authors: Xue-fu You, Wei-xi TianAbstract:It is important to develop new antibiotics aimed at novel targets. The investigation found that the Leaf Extracts from five maples (Acer platanoides, Acer campestre, Acer rubrum, Acer saccharum and Acer truncatum Bunge collected in Denmark, Canada and China) and their component tannic acid displayed antibacterial ability against 24 standard bacteria strains with the minimum inhibitory concentration of 0.3–8.0 mg/mL. Unlike the standard antibiotic levofloxacin (LFX), these samples inhibited Gram-positive bacteria more effectively than they inhibited Gram-negative bacteria. These samples effectively inhibited two antidrug bacterial strains. The results show that these samples inhibit bacteria by a different mechanism from LFX. These samples potently inhibited b-ketoacyl-ACP reductase (FabG), which is an important enzyme in bacterial fatty acid synthesis. Tannic acid showed the strongest inhibition on FabG with a half inhibition concentration of 0.78 mm (0.81 mg/mL). Furthermore, tannic acid and two maple Leaf Extracts showed time-dependent irreversible inhibition of FabG. These three samples also exhibited better inhibition on bacteria. It is suggested that FabG is the antibacteria target of maple Leaf Extracts and tannic acid, and both reversible and irreversible inhibitions of FabG are important for the antibacterial effect. Copyright © 2009 John Wiley & Sons, Ltd.
Jae Yong Song - One of the best experts on this subject based on the ideXlab platform.
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biological synthesis of gold nanoparticles using magnolia kobus and diopyros kaki Leaf Extracts
Process Biochemistry, 2009Co-Authors: Jae Yong Song, Hyeonkyeong Jang, Beom Soo KimAbstract:Abstract Leaf Extracts of two plants, Magnolia kobus and Diopyros kaki , were used for ecofriendly extracellular synthesis of metallic gold nanoparticles. Stable gold nanoparticles were formed by treating an aqueous HAuCl 4 solution using the plant Leaf Extracts as reducing agents. UV–visible spectroscopy was used for quantification of gold nanoparticle synthesis. Only a few minutes were required for >90% conversion to gold nanoparticles at a reaction temperature of 95 °C, suggesting reaction rates higher or comparable to those of nanoparticle synthesis by chemical methods. The synthesized gold nanoparticles were characterized with inductively coupled plasma spectrometry (ICP), energy-dispersive X-ray spectroscopy (EDS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), and particle analysis using a particle analyzer. SEM and TEM images showed that a mixture of plate (triangles, pentagons, and hexagons) and spherical structures (size, 5–300 nm) were formed at lower temperatures and Leaf broth concentrations, while smaller spherical shapes were obtained at higher temperatures and Leaf broth concentrations.
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Rapid biological synthesis of silver nanoparticles using plant Leaf Extracts
Bioprocess and Biosystems Engineering, 2009Co-Authors: Jae Yong Song, Beom Soo KimAbstract:Five plant Leaf Extracts (Pine, Persimmon, Ginkgo, Magnolia and Platanus) were used and compared for their extracellular synthesis of metallic silver nanoparticles. Stable silver nanoparticles were formed by treating aqueous solution of AgNO3 with the plant Leaf Extracts as reducing agent of Ag+ to Ag0. UV-visible spectroscopy was used to monitor the quantitative formation of silver nanoparticles. Magnolia Leaf broth was the best reducing agent in terms of synthesis rate and conversion to silver nanoparticles. Only 11 min was required for more than 90% conversion at the reaction temperature of 95 °C using Magnolia Leaf broth. The synthesized silver nanoparticles were characterized with inductively coupled plasma spectrometry (ICP), energy dispersive X-ray spectroscopy (EDS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and particle analyzer. The average particle size ranged from 15 to 500 nm. The particle size could be controlled by changing the reaction temperature, Leaf broth concentration and AgNO3 concentration. This environmentally friendly method of biological silver nanoparticles production provides rates of synthesis faster or comparable to those of chemical methods and can potentially be used in various human contacting areas such as cosmetics, foods and medical applications.