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John B. Golding - One of the best experts on this subject based on the ideXlab platform.
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Interaction of the hydrogen sulphide inhibitor, propargylglycine (PAG), with hydrogen sulphide on postharvest changes of the green Leafy Vegetable, pak choy
Postharvest Biology and Technology, 2019Co-Authors: H. M.s. Al Ubeed, R B H Wills, Michael C. Bowyer, John B. GoldingAbstract:Propargylglycine (PAG) is an inhibitor of hydrogen sulphide (H2S) production and has been used to explore the mode of action of H2S in prolonging storage of horticultural produce but little attention has been given to how PAG and H2S interact when both are applied to produce. This study examined the effect of sequential application of PAG and H2S on a range of postharvest senescence factors of the Leafy Vegetable pak choy (Brassica rapa subsp. Chinensis) stored at 10 °C. The results showed differential responses between factors when compared to application of PAG or H2S alone. As expected, fumigation with H2S reduced the rate of loss of leaf green colour, respiration rate, ethylene production, ion leakage and enhanced antioxidant activity and leaves sprayed with PAG showing converse effects. If PAG acted solely by inhibiting endogenous H2S production then subsequent treatment with H2S should fully negate any effect induced by PAG. However, for the combined PAG + H2S treatment, respiration was similar leaves fumigated with H2S, loss of green leaf colour was similar to the PAG single treatment and less than the untreated control, antioxidant activity was less than for PAG but greater than for control leaves, and ethylene production and ion leakage were similar to control leaves. Thus, the concept that PAG is exclusively an inhibitor of endogenous H2S production was not validated, with PAG having effects on metabolism that are not linked to the action of endogenous H2S. The additional actions of PAG could be through its inhibition of pyridoxyal-5′-phosphate (PHP) which is a coenzyme for numerous enzyme systems.
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Comparison of hydrogen sulphide with 1-methylcyclopropene (1-MCP) to inhibit senescence of the Leafy Vegetable, pak choy
Postharvest Biology and Technology, 2018Co-Authors: H. M.s. Al Ubeed, R B H Wills, Michael C. Bowyer, John B. GoldingAbstract:Postharvest fumigation with hydrogen sulphide (H2S) can inhibit senescence of a range of fruit and Vegetables. It has been suggested that the mode of action of hydrogen sulphide is through inhibiting either the endogenous production and/or the action of ethylene. This study compared the effect of fumigation with 250 μL L−1 hydrogen sulphide and 10 μL L−1 1-methylcyclopropene (1-MCP) on a range of factors associated with senesence of the Leafy Vegetable, pak choy stored at 10 °C and ventilated with ethylene-free air or air containing 0.1 μL L−1 ethylene. When pak choy was stored in an ethylene-free atmosphere, hydrogen sulphide and 1-MCP were equally effective in inhibiting the loss of green colour, respiration, ion leakage and endogenous ethylene production. However, for containers ventilated with ethylene, 1-MCP was more effective in inhibiting loss of green colour, respiration and ethylene production than hydrogen sulphide. Sequential fumigation with 1-MCP followed by hydrogen sulphide showed no difference in any quality factor to produce fumigated with 1-MCP. The study concluded that (i) the mode of action of hydrogen sulphide included inhibiting the action of ethylene and (ii) for pak choy, 1-MCP was a more effective fumigation treatment than hydrogen sulphide.
H. M.s. Al Ubeed - One of the best experts on this subject based on the ideXlab platform.
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Interaction of the hydrogen sulphide inhibitor, propargylglycine (PAG), with hydrogen sulphide on postharvest changes of the green Leafy Vegetable, pak choy
Postharvest Biology and Technology, 2019Co-Authors: H. M.s. Al Ubeed, R B H Wills, Michael C. Bowyer, John B. GoldingAbstract:Propargylglycine (PAG) is an inhibitor of hydrogen sulphide (H2S) production and has been used to explore the mode of action of H2S in prolonging storage of horticultural produce but little attention has been given to how PAG and H2S interact when both are applied to produce. This study examined the effect of sequential application of PAG and H2S on a range of postharvest senescence factors of the Leafy Vegetable pak choy (Brassica rapa subsp. Chinensis) stored at 10 °C. The results showed differential responses between factors when compared to application of PAG or H2S alone. As expected, fumigation with H2S reduced the rate of loss of leaf green colour, respiration rate, ethylene production, ion leakage and enhanced antioxidant activity and leaves sprayed with PAG showing converse effects. If PAG acted solely by inhibiting endogenous H2S production then subsequent treatment with H2S should fully negate any effect induced by PAG. However, for the combined PAG + H2S treatment, respiration was similar leaves fumigated with H2S, loss of green leaf colour was similar to the PAG single treatment and less than the untreated control, antioxidant activity was less than for PAG but greater than for control leaves, and ethylene production and ion leakage were similar to control leaves. Thus, the concept that PAG is exclusively an inhibitor of endogenous H2S production was not validated, with PAG having effects on metabolism that are not linked to the action of endogenous H2S. The additional actions of PAG could be through its inhibition of pyridoxyal-5′-phosphate (PHP) which is a coenzyme for numerous enzyme systems.
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Comparison of hydrogen sulphide with 1-methylcyclopropene (1-MCP) to inhibit senescence of the Leafy Vegetable, pak choy
Postharvest Biology and Technology, 2018Co-Authors: H. M.s. Al Ubeed, R B H Wills, Michael C. Bowyer, John B. GoldingAbstract:Postharvest fumigation with hydrogen sulphide (H2S) can inhibit senescence of a range of fruit and Vegetables. It has been suggested that the mode of action of hydrogen sulphide is through inhibiting either the endogenous production and/or the action of ethylene. This study compared the effect of fumigation with 250 μL L−1 hydrogen sulphide and 10 μL L−1 1-methylcyclopropene (1-MCP) on a range of factors associated with senesence of the Leafy Vegetable, pak choy stored at 10 °C and ventilated with ethylene-free air or air containing 0.1 μL L−1 ethylene. When pak choy was stored in an ethylene-free atmosphere, hydrogen sulphide and 1-MCP were equally effective in inhibiting the loss of green colour, respiration, ion leakage and endogenous ethylene production. However, for containers ventilated with ethylene, 1-MCP was more effective in inhibiting loss of green colour, respiration and ethylene production than hydrogen sulphide. Sequential fumigation with 1-MCP followed by hydrogen sulphide showed no difference in any quality factor to produce fumigated with 1-MCP. The study concluded that (i) the mode of action of hydrogen sulphide included inhibiting the action of ethylene and (ii) for pak choy, 1-MCP was a more effective fumigation treatment than hydrogen sulphide.
Johannes Van Staden - One of the best experts on this subject based on the ideXlab platform.
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nutritional and pharmacological potential of the genus ceratotheca an underutilized Leafy Vegetable of africa
Journal of Ethnopharmacology, 2016Co-Authors: Nqobile A Masondo, J F Finnie, Johannes Van StadenAbstract:Abstract Ethnopharmacological relevance Ceratotheca (Pedaliaceae) is an endemic African genus comprising of five species. The genus is commonly used as a Leafy Vegetable with medicinal properties. Aim of the review The review aims to highlight the unexplored nutritional and pharmacological potential of African indigenous Leafy Vegetables Ceratotheca sesamoides and triloba, in order to conserve and domesticate these species. Methods The information was obtained from various search engines such as Scopus, Google Scholar and Web of Science as well as Ethnobotanical books. Results Ceratotheca sesamoides and triloba have good nutritional potential. The species are high in energy levels, fat content, proteins and carbohydrate values. The species have also been reported to have good antibacterial, antidiarrhoeal, antidiabetic, antiplasmodial and antiviral properties. These species have slight toxicity and cytotoxic activity when extracted at high concentrations but no mutagenic activity was detected. Conclusions To date, few studies have documented the usage (nutrition and pharmacology) of Ceratotheca sesamoides and triloba. More studies investigating the nutritional content as well as methods of its improvement are necessary if the plant is to be included as a domesticated Vegetable crop. Safety and toxicity analysis of this Leafy Vegetable need to be extensively studied as the plants are consumed in high quantities.
Ambaliou Sanni - One of the best experts on this subject based on the ideXlab platform.
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antimicrobial antioxidant toxicity and phytochemical assessment of extracts from acmella uliginosa a Leafy Vegetable consumed in benin west africa
BMC Complementary and Alternative Medicine, 2016Co-Authors: Latifou Lagnika, Abdou Madjid O Amoussa, Rafatou Adedoyin A Adjileye, Anatole Laleye, Ambaliou SanniAbstract:Acmella uliginosa (Asteraceae) is a flowering plant whose leaves are consumed as a Vegetable in Benin. They are also traditionally used as an antibiotic in the treatment of infectious diseases. To evaluate the therapeutic potential and toxicity effect of this Leafy-Vegetable, the antibacterial, antifungal, antioxidant activities and, toxicity and phytochemical constituents were investigated. Dichloromethane, methanol and aqueous extracts of Acmella uliginosa were evaluated for their antimicrobial activity against six bacterial and six fungi strains. Antibacterial and antifungal activities were investigated by microdilution method and agar diffusion method respectively. Antioxidant activity was assessed using the 2,2-diphenyl-1-picryl-hydrazyl assay and phytochemical screening was carried out using standard procedures. Finally, oral acute toxicity at a dose of 2000 mg/kg was done according to the Organization for Economic Co-operation and Development guideline n° 423. The antibacterial activity was broad spectrum, inhibiting both Gram-positive and Gram-negative bacteria. The minimum inhibitory concentration ranged from 0.625 to 5 mg/ml. The antifungal evaluation show that all the extracts inhibited mycelial growth and sporulation of fungi with percentages of inhibition ranging from 9.39 to 75.67 % and 22.04 to 99.77 %, respectively. In DPPH radical scavenging assay, the effect on reducing free radicals increased in a dose dependent manner. The percentage of inhibition of DPPH ranged from 0.94 to 73.07 %. Phytochemical screening revealed the presence of coumarin, flavonoid, naphtoquinone, anthracene derivative, saponin, lignan, triterpene and tannin. The dichloromethane and methanol extracts showed the best biological activities; they were also shown as the best extraction solvents of phytochemicals. In the acute toxicity evaluation, all animals were physically active and no deaths of rats were observed during the test. However, the aqueous extract promoted biochemical, hematological and histopathological alterations of treated rats at 2000 mg/kg body weight. A. uliginosa extracts contains antimicrobial, antioxidant agents and was not lethal for rats when ingested. However, according to the results obtained for biochemical, hematological, and histopathological analysis, caution is required regarding its consumption.
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agromorphological characterization of sesamum radiatum schum and thonn a neglected and underutilized species of traditional Leafy Vegetable of great importance in benin
African Journal of Agricultural Research, 2012Co-Authors: Kifouli Adeoti, A Dansi, Leonard E Ahoton, R Vodouhe, B C Ahohuendo, Alain Rival, Ambaliou SanniAbstract:Sesamum radiatum (Schum. and Thonn) is a traditional Leafy Vegetable of national importance in Benin. Although, it is cultivated and consumed in many regions of the country, it is still unfortunately neglected by scientific research. In order to fill in the knowledge gaps necessary for its varietal improvement, 16 accessions collected from different agro-ecological zones were characterized using 16 quantitative morphological traits. A significant variability was found among the accessions. Day to flowering, day to fruiting, total biomass, plant height, noose diameter, number of fruits per plant and number of seeds per fruit were some of the most discriminating parameters. Multivariate analysis (principal component analysis and cluster analysis) revealed four distinct agro-morphological groups of varying performance which will be highly useful in varietal improvement. Correlation analyses between descriptors revealed uniquely desired positive and negative relationships hence, indicating predictable success for eventual breeding program. To further investigate the genetic basis of the phenotypic diversity revealed among S. radiatum accessions, the use of molecular markers such as amplified fragment length polymorphisms (AFLPs) is recommended. (Resume d'auteur)
Michael C. Bowyer - One of the best experts on this subject based on the ideXlab platform.
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Interaction of the hydrogen sulphide inhibitor, propargylglycine (PAG), with hydrogen sulphide on postharvest changes of the green Leafy Vegetable, pak choy
Postharvest Biology and Technology, 2019Co-Authors: H. M.s. Al Ubeed, R B H Wills, Michael C. Bowyer, John B. GoldingAbstract:Propargylglycine (PAG) is an inhibitor of hydrogen sulphide (H2S) production and has been used to explore the mode of action of H2S in prolonging storage of horticultural produce but little attention has been given to how PAG and H2S interact when both are applied to produce. This study examined the effect of sequential application of PAG and H2S on a range of postharvest senescence factors of the Leafy Vegetable pak choy (Brassica rapa subsp. Chinensis) stored at 10 °C. The results showed differential responses between factors when compared to application of PAG or H2S alone. As expected, fumigation with H2S reduced the rate of loss of leaf green colour, respiration rate, ethylene production, ion leakage and enhanced antioxidant activity and leaves sprayed with PAG showing converse effects. If PAG acted solely by inhibiting endogenous H2S production then subsequent treatment with H2S should fully negate any effect induced by PAG. However, for the combined PAG + H2S treatment, respiration was similar leaves fumigated with H2S, loss of green leaf colour was similar to the PAG single treatment and less than the untreated control, antioxidant activity was less than for PAG but greater than for control leaves, and ethylene production and ion leakage were similar to control leaves. Thus, the concept that PAG is exclusively an inhibitor of endogenous H2S production was not validated, with PAG having effects on metabolism that are not linked to the action of endogenous H2S. The additional actions of PAG could be through its inhibition of pyridoxyal-5′-phosphate (PHP) which is a coenzyme for numerous enzyme systems.
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Comparison of hydrogen sulphide with 1-methylcyclopropene (1-MCP) to inhibit senescence of the Leafy Vegetable, pak choy
Postharvest Biology and Technology, 2018Co-Authors: H. M.s. Al Ubeed, R B H Wills, Michael C. Bowyer, John B. GoldingAbstract:Postharvest fumigation with hydrogen sulphide (H2S) can inhibit senescence of a range of fruit and Vegetables. It has been suggested that the mode of action of hydrogen sulphide is through inhibiting either the endogenous production and/or the action of ethylene. This study compared the effect of fumigation with 250 μL L−1 hydrogen sulphide and 10 μL L−1 1-methylcyclopropene (1-MCP) on a range of factors associated with senesence of the Leafy Vegetable, pak choy stored at 10 °C and ventilated with ethylene-free air or air containing 0.1 μL L−1 ethylene. When pak choy was stored in an ethylene-free atmosphere, hydrogen sulphide and 1-MCP were equally effective in inhibiting the loss of green colour, respiration, ion leakage and endogenous ethylene production. However, for containers ventilated with ethylene, 1-MCP was more effective in inhibiting loss of green colour, respiration and ethylene production than hydrogen sulphide. Sequential fumigation with 1-MCP followed by hydrogen sulphide showed no difference in any quality factor to produce fumigated with 1-MCP. The study concluded that (i) the mode of action of hydrogen sulphide included inhibiting the action of ethylene and (ii) for pak choy, 1-MCP was a more effective fumigation treatment than hydrogen sulphide.