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Salvador Gonzalezb - One of the best experts on this subject based on the ideXlab platform.

Artur Zembowicz - One of the best experts on this subject based on the ideXlab platform.

  • Expression of Soluble Adenylyl Cyclase in Lentigo Maligna: Use of Immunohistochemistry With Anti–Soluble Adenylyl Cyclase Antibody (R21) in Diagnosis of Lentigo Maligna and Assessment of Margins
    Archives of pathology & laboratory medicine, 2012
    Co-Authors: Cynthia M. Magro, Sung-eun Yang, Jonathan H. Zippin, Artur Zembowicz
    Abstract:

    Context.—Soluble adenylyl cyclase (sAC) is an enzyme that generates cyclic adenosine monophosphate, a signaling molecule involved in regulating melanocyte functions. R21, a mouse monoclonal antibody against sAC, shows a striking pan-nuclear staining in Lentigo Maligna, indicating possible utility for diagnosis and margin assessment. Objective.—To evaluate R21 in the diagnosis and evaluation of margins in Lentigo Maligna. Design.—Thirty one re-excision specimens for Lentigo Maligna were evaluated for R21 expression using previously published protocol. In addition, 153 cases including 41 Lentigo Malignas, 30 non–Lentigo Maligna-type melanomas, 38 Lentigos, and 44 nevi were evaluated using a modified stringent protocol to eliminate all nonmelanocyte staining. Results.—The sensitivity of nuclear staining with R21 in Lentigo Maligna was 87.8%. Nuclear expression of sAC was observed in 40% of other melanomas and 2.3% of benign nevi. R21 did not stain nuclei of resting melanocytes but was observed in 28.9% of me...

  • expression of soluble adenylyl cyclase in Lentigo Maligna use of immunohistochemistry with anti soluble adenylyl cyclase antibody r21 in diagnosis of Lentigo Maligna and assessment of margins
    Archives of Pathology & Laboratory Medicine, 2012
    Co-Authors: Cynthia M. Magro, Sung-eun Yang, Jonathan H. Zippin, Artur Zembowicz
    Abstract:

    Context.—Soluble adenylyl cyclase (sAC) is an enzyme that generates cyclic adenosine monophosphate, a signaling molecule involved in regulating melanocyte functions. R21, a mouse monoclonal antibody against sAC, shows a striking pan-nuclear staining in Lentigo Maligna, indicating possible utility for diagnosis and margin assessment. Objective.—To evaluate R21 in the diagnosis and evaluation of margins in Lentigo Maligna. Design.—Thirty one re-excision specimens for Lentigo Maligna were evaluated for R21 expression using previously published protocol. In addition, 153 cases including 41 Lentigo Malignas, 30 non–Lentigo Maligna-type melanomas, 38 Lentigos, and 44 nevi were evaluated using a modified stringent protocol to eliminate all nonmelanocyte staining. Results.—The sensitivity of nuclear staining with R21 in Lentigo Maligna was 87.8%. Nuclear expression of sAC was observed in 40% of other melanomas and 2.3% of benign nevi. R21 did not stain nuclei of resting melanocytes but was observed in 28.9% of me...

Zeina B S Tannousa - One of the best experts on this subject based on the ideXlab platform.

Martin C Mihma - One of the best experts on this subject based on the ideXlab platform.

Thomas J Flottea - One of the best experts on this subject based on the ideXlab platform.