The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Brian Gratwicke - One of the best experts on this subject based on the ideXlab platform.
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Minimum Lethal Concentration of sodium hypochlorite for the amphibian pathogen Batrachochytrium dendrobatidis.
PLOS ONE, 2017Co-Authors: Matthew H. Becker, Brian GratwickeAbstract:Sodium hypochlorite (NaOCl) is the active ingredient in household bleach and is commonly used as a disinfectant to clean equipment contaminated by the amphibian pathogen Batrachochytrium dendrobatidis (Bd) in lab husbandry and field studies. We conducted a series of replicated exposure trials using a single Global Pandemic Lineage Bd isolate from Panama (JEL 310) and Concentrations of NaOCl ranging from 0.006% to 0.6% for exposure times ranging from 30 seconds to 15 minutes to determine the minimum Lethal Concentration of NaOCl for this isolate of Bd. Sodium hypochlorite completely killed Bd at a Concentration of 0.03% during a 15-minute exposure time, while 0.12% NaOCl was effective at all exposure times (30s-15min).
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Minimum Lethal Concentration of sodium hypochlorite for the amphibian pathogen Batrachochytrium dendrobatidis.
PLOS ONE, 2017Co-Authors: Matthew H. Becker, Brian GratwickeAbstract:Sodium hypochlorite (NaOCl) is the active ingredient in household bleach and is commonly used as a disinfectant to clean equipment contaminated by the amphibian pathogen Batrachochytrium dendrobatidis (Bd) in lab husbandry and field studies. We conducted a series of replicated exposure trials using a single Global Pandemic Lineage Bd isolate from Panama (JEL 310) and Concentrations of NaOCl ranging from 0.006% to 0.6% for exposure times ranging from 30 seconds to 15 minutes to determine the minimum Lethal Concentration of NaOCl for this isolate of Bd. Sodium hypochlorite completely killed Bd at a Concentration of 0.03% during a 15-minute exposure time, while 0.12% NaOCl was effective at all exposure times (30s-15min).
Matthew H. Becker - One of the best experts on this subject based on the ideXlab platform.
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Minimum Lethal Concentration of sodium hypochlorite for the amphibian pathogen Batrachochytrium dendrobatidis.
PLOS ONE, 2017Co-Authors: Matthew H. Becker, Brian GratwickeAbstract:Sodium hypochlorite (NaOCl) is the active ingredient in household bleach and is commonly used as a disinfectant to clean equipment contaminated by the amphibian pathogen Batrachochytrium dendrobatidis (Bd) in lab husbandry and field studies. We conducted a series of replicated exposure trials using a single Global Pandemic Lineage Bd isolate from Panama (JEL 310) and Concentrations of NaOCl ranging from 0.006% to 0.6% for exposure times ranging from 30 seconds to 15 minutes to determine the minimum Lethal Concentration of NaOCl for this isolate of Bd. Sodium hypochlorite completely killed Bd at a Concentration of 0.03% during a 15-minute exposure time, while 0.12% NaOCl was effective at all exposure times (30s-15min).
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Minimum Lethal Concentration of sodium hypochlorite for the amphibian pathogen Batrachochytrium dendrobatidis.
PLOS ONE, 2017Co-Authors: Matthew H. Becker, Brian GratwickeAbstract:Sodium hypochlorite (NaOCl) is the active ingredient in household bleach and is commonly used as a disinfectant to clean equipment contaminated by the amphibian pathogen Batrachochytrium dendrobatidis (Bd) in lab husbandry and field studies. We conducted a series of replicated exposure trials using a single Global Pandemic Lineage Bd isolate from Panama (JEL 310) and Concentrations of NaOCl ranging from 0.006% to 0.6% for exposure times ranging from 30 seconds to 15 minutes to determine the minimum Lethal Concentration of NaOCl for this isolate of Bd. Sodium hypochlorite completely killed Bd at a Concentration of 0.03% during a 15-minute exposure time, while 0.12% NaOCl was effective at all exposure times (30s-15min).
Sevdan Yilmaz - One of the best experts on this subject based on the ideXlab platform.
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acute exposure to boron in nile tilapia oreochromis niloticus median Lethal Concentration lc50 blood parameters dna fragmentation of blood and sperm cells
Chemosphere, 2018Co-Authors: Umit Acar, Burak Evren Inanan, Fahriye Zemheri, Osman Sabri Kesbic, Sevdan YilmazAbstract:Abstract Recently, Boron (B) contamination of aquatic ecosystem has received considerable critical attention due to its toxic effects at high Concentrations on plants as well as animals. Previous studies on toxic effects of B have not dealt with DNA damages in blood and sperm cells of Nile tilapia (Oreochromis niloticus), together with blood parameters. This study consisted of two successive experiments purposes to determine these findings, including the median-Lethal Concentration (LC50) of B for Nile tilapia. In the first one, at 96 h, LC50 of B for Nile tilapia was 141.42 mg L−1 B. In the second one, Nile tilapia were treated with five elevated B Concentrations (1, 5, 25, 50 and 100 mg L−1 B) and hematological, serum biochemical parameters, and DNA damages of Nile tilapia in these treatments were determined comparing with the control after 14 days of exposure. Hematological parameters (red blood cell count, hematocrit, and hemoglobin) were similar to each other in all groups while serum biochemical parameters (glucose, total protein, cholesterol, alkaline phosphatase, glutamic oxaloacetic transaminase, and glutamic pyruvic transaminase) in B treatments, especially in 50 and 100 mg L−1 B groups, were found significantly higher (P
Umit Acar - One of the best experts on this subject based on the ideXlab platform.
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acute exposure to boron in nile tilapia oreochromis niloticus median Lethal Concentration lc50 blood parameters dna fragmentation of blood and sperm cells
Chemosphere, 2018Co-Authors: Umit Acar, Burak Evren Inanan, Fahriye Zemheri, Osman Sabri Kesbic, Sevdan YilmazAbstract:Abstract Recently, Boron (B) contamination of aquatic ecosystem has received considerable critical attention due to its toxic effects at high Concentrations on plants as well as animals. Previous studies on toxic effects of B have not dealt with DNA damages in blood and sperm cells of Nile tilapia (Oreochromis niloticus), together with blood parameters. This study consisted of two successive experiments purposes to determine these findings, including the median-Lethal Concentration (LC50) of B for Nile tilapia. In the first one, at 96 h, LC50 of B for Nile tilapia was 141.42 mg L−1 B. In the second one, Nile tilapia were treated with five elevated B Concentrations (1, 5, 25, 50 and 100 mg L−1 B) and hematological, serum biochemical parameters, and DNA damages of Nile tilapia in these treatments were determined comparing with the control after 14 days of exposure. Hematological parameters (red blood cell count, hematocrit, and hemoglobin) were similar to each other in all groups while serum biochemical parameters (glucose, total protein, cholesterol, alkaline phosphatase, glutamic oxaloacetic transaminase, and glutamic pyruvic transaminase) in B treatments, especially in 50 and 100 mg L−1 B groups, were found significantly higher (P
E W Taylor - One of the best experts on this subject based on the ideXlab platform.
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exposure of brown trout salmo trutta to a sub Lethal Concentration of copper in soft acidic water effects upon muscle metabolism and membrane potential
Aquatic Toxicology, 2000Co-Authors: M W Beaumont, P J Butler, E W TaylorAbstract:Brown trout acclimated to soft water and exposed for 96 h to a sub-Lethal Concentration of copper at low pH (0.08 μmol l−1 Cu, pH 5) have a lower critical swimming speed than fish from copper-free water at neutral pH. This loss of performance is not due to difficulties in oxygen transfer resulting from gill damage since arterial oxygen and carbon dioxide levels remain unaffected. Both red and white muscle showed some metabolic disruptions consistent with local hypoxia, namely a high lactate Concentration at rest and, in the white muscle, depletion of glycogen and phosphocreatine. However, a putative role of increased blood viscosity following haematological changes in reducing the supply of oxygen to the tissues is not supported by the current study. Haematocrit, haemoglobin and plasma protein Concentrations were not affected by this treatment and a lack of further change in variables such as lactate at the onset of exercise led one to look for an alternative explanation for the effects of copper and low pH upon tissue metabolites. Ammonia Concentration, both in the plasma and muscles, is significantly higher in trout exposed to copper and low pH. Ammonia plays a role in the regulation of a number of metabolic pathways and could contribute to the altered metabolic status of these fish. In addition, ammonium ions are known to cause electrophysiological disruptions, particularly the displacement of K+ in ion exchange mechanisms that could lead to the observed loss of swimming performance. Using the measured distribution of ammonia between intracellular and extracellular compartments to estimate membrane potential of resting muscle, a significant depolarisation is predicted in both red and white muscle of fish exposed to copper and low pH.