Sodium Hypochlorite

14,000,000 Leading Edge Experts on the ideXlab platform

Scan Science and Technology

Contact Leading Edge Experts & Companies

Scan Science and Technology

Contact Leading Edge Experts & Companies

The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform

Luciano Giardino - One of the best experts on this subject based on the ideXlab platform.

Zahed Mohammadi - One of the best experts on this subject based on the ideXlab platform.

Flavio Palazzi - One of the best experts on this subject based on the ideXlab platform.

R. Perrin - One of the best experts on this subject based on the ideXlab platform.

  • Efficacy and mechanisms of action of Sodium Hypochlorite on Pseudomonas aeruginosa PAO1 phage F116
    Journal of Applied Microbiology, 1998
    Co-Authors: Jean-yves Maillard, A. C. Hann, V. Baubet, R. Perrin
    Abstract:

    The Pseudomonas aeruginosa PAO1 phage F116 was used to investigate the viricidal activity and the mechanism of action of Sodium Hypochlorite. The bacteriophage was inactivated with a low concentration (0.0005% available chlorine) of the biocide prepared in tap water but it was less sensitive to a Sodium Hypochlorite solution prepared in ultra-pure water (0.0075% available chlorine). For all the effective concentrations of Sodium Hypochlorite (i.e. producing at least 4 log reduction in phage titre), F116 was readily inactivated within 30 s. Electron microscopical investigations of the phage particles challenged with Sodium Hypochlorite showed a wide variety of deleterious effects, some of which have not been previously observed with other biocides. The wide range of structural alterations observed suggested that Sodium Hypochlorite has multiple target sites against F116 bacteriophage. A 30 s exposure to Sodium Hypochlorite (0.001% available chlorine) produced severe damage, the number and severity of which increased with a higher concentration (0.0075% available chlorine) and with a longer contact time. These observations suggested that Sodium Hypochlorite inactivated F116 bacteriophage by causing structural alterations to the phage head, tail and overall structure, hence possibly releasing the viral genome from damaged capsids in the surrounding media.

  • Resistance of Pseudomonas aeruginosa PAO1 phage F116 to Sodium Hypochlorite
    Journal of Applied Microbiology, 1998
    Co-Authors: Jean-yves Maillard, A. C. Hann, R. Perrin
    Abstract:

    The development of viral resistance to Sodium Hypochlorite was investigated using the Pseudomonas aeruginosa bacteriophage F116 as a model system. This phage was chosen because of its structural characteristics and former investigations conducted in this laboratory. F116 was shown to be sensitive to a Sodium Hypochlorite concentration of 0.0075 gl-1 (available chlorine) which produced a 5 log10 reduction in titre in a suspension test. Survival bacteriophages challenged with this Sodium Hypochlorite concentration were isolated, propagated and challenged again with the same and higher concentrations of the biocide. It was observed that progeny virions were becoming increasingly resistant to Sodium Hypochlorite challenges up to a concentration of 0.0175 gl-1 of available chlorine. It was also noticed that 1-2 log10 of F116 virions from resistant phage lysates remained sensitive to the biocide. An electron microscopical investigation of F116 resistant lysates showed that the phage resistance to Sodium Hypochlorite was not caused by F116 particles aggregation. Furthermore, no morphological difference between the sensitive and resistant F116 particles to Sodium Hypochlorite was identified.

Simone Grandini - One of the best experts on this subject based on the ideXlab platform.