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Christoph Oberprieler - One of the best experts on this subject based on the ideXlab platform.
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Karyological and molecular analysis of Leucanthemum (Compositae, Anthemideae) in Corsica
Willdenowia, 2019Co-Authors: Christoph Oberprieler, Florian Wagner, Lena Schinhärl, Laetitia Hugot, Robert VogtAbstract:Karyological, flow-cytometric and molecular analyses indicate that the genus Leucanthemum Mill. (Compositae, Anthemideae) is represented in Corsica (Corse) by two species: the tetraploid L. ircutianum DC. and the hexaploid L. corsicum (Less.) DC. The indication of the occurrence of the diploid L. vulgare Lam. on the island and of a tetraploid chromosome number for L. corsicum, given in former treatments of the genus for Corsica, could not be corroborated. AFLP fingerprinting further suggests that the infraspecific taxonomy of L. corsicum with two subspecies (L. corsicum subsp. corsicum and subsp. fenzlii) and three forms (L. corsicum f. corsicum, f. pinnatifidum and f. eschenlohrianum), which is mainly based on differences in the degree of leaf dissection, is not backed by genetic discontinuities. Owing to the observed little variation in leaf dissection within populations in the wild and the constancy of these features under cultivation, we propose the rank of varieties to taxonomically acknowledge these different stages in the broad spectrum of leaf-dissection grades exhibited by L. corsicum. As a consequence, the two new combinations L. corsicum var. eschenlohrianum (Gamisans) Vogt, Hugot & Oberpr. and L. corsicum var. fenzlii (Gamisans) Vogt, Hugot & Oberpr. are proposed.
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'At the crossroads towards polyploidy': genomic divergence and extent of homoploid hybridization are drivers for the formation of the ox-eye daisy polyploid complex (Leucanthemum, Compositae-Anthemideae).
The New phytologist, 2019Co-Authors: Florian Wagner, Robert Vogt, Tankred Ott, Claudia Zimmer, Verena Reichhart, Christoph OberprielerAbstract:Polyploidy plays a paramount role in phytodiversity, but the causes of this evolutionary pathway require further study. Here, we use phylogenetic methods to examine possible polyploidy-promoting factors by comparing diploid representatives of the comprehensive European polyploid complex Leucanthemum with members of its strictly diploid North African counterpart Rhodanthemum. We investigate genetic divergence and gene flow among all diploid lineages of both genera to evaluate the role of genomic differentiation and hybridization for polyploid speciation. To test whether hybridization in Leucanthemum has been triggered by the geological conditions during its diversification, we additionally generate a time-calibrated phylogeny of 46 species of the subtribe Leucantheminae. Leucanthemum shows a significantly higher genetic divergence and hybridization signal among diploid lineages compared with Rhodanthemum, in spite of a similar crown age and diversification pattern during the Quaternary. Our study demonstrates the importance of genetic differentiation among diploid progenitors and their concurrent affinity for natural hybridization for the formation of a polyploid complex. Furthermore, the role of climate-induced range overlaps on hybridization and polyploid speciation during the Quaternary is discussed.
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Karyological analysis reveals two new polyploid marguerite taxa (Leucanthemum, Compositae–Anthemideae) in S France and NW Italy
Willdenowia, 2018Co-Authors: Robert Vogt, Kamil Konowalik, Christoph OberprielerAbstract:New chromosome counts suggest that the former Leucanthemum vulgare var. esterellense Briq. & Cavill. deserves acknowledgement as an independent species. The new combination L. esterellense (Briq. & Cavill) Vogt, Konowalik & Oberpr., proposed here, denotes a marguerite species endemic to the Massif de l'Esterel (S France) with an octoploid chromosome number of 2n = 72. It is also demonstrated that recently formed hybrids between L. glaucophyllum and L. pallens at Mt Bignone (Liguria, NW Italy) exhibit hepta- and octoploid chromosome numbers. These hybrids are described as L. ×marchii Konowalik, Vogt & Oberpr., a nothospecies new to science. Additionally, the names L. subglaucum de Laramb., L. vulgare var. esterellense Briq. & Cavill. and L. vulgare subsp. glaucophyllum Briq. & Cavill. are lectotypified and new chromosome counts for all presently surveyed Leucanthemum taxa are provided.Citation: Karyological analysis reveals two new polyploid marguerite taxa (Leucanthemum, Compositae–Anthemideae) in S France and NW Italy. – Willdenowia 48: 221–226. doi: https://doi.org/10.3372/wi.48.48204Version of record first published online on 3 August 2018 ahead of inclusion in August 2018 issue.
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karyological analysis reveals two new polyploid marguerite taxa Leucanthemum compositae anthemideae in s france and nw italy
Willdenowia, 2018Co-Authors: Robert Vogt, Kamil Konowalik, Christoph OberprielerAbstract:New chromosome counts suggest that the former Leucanthemum vulgare var. esterellense Briq. & Cavill. deserves acknowledgement as an independent species. The new combination L. esterellense (Briq. & Cavill) Vogt, Konowalik & Oberpr., proposed here, denotes a marguerite species endemic to the Massif de l'Esterel (S France) with an octoploid chromosome number of 2n = 72. It is also demonstrated that recently formed hybrids between L. glaucophyllum and L. pallens at Mt Bignone (Liguria, NW Italy) exhibit hepta- and octoploid chromosome numbers. These hybrids are described as L. ×marchii Konowalik, Vogt & Oberpr., a nothospecies new to science. Additionally, the names L. subglaucum de Laramb., L. vulgare var. esterellense Briq. & Cavill. and L. vulgare subsp. glaucophyllum Briq. & Cavill. are lectotypified and new chromosome counts for all presently surveyed Leucanthemum taxa are provided.Citation: Karyological analysis reveals two new polyploid marguerite taxa (Leucanthemum, Compositae–Anthemideae) in S France and NW Italy. – Willdenowia 48: 221–226. doi: https://doi.org/10.3372/wi.48.48204Version of record first published online on 3 August 2018 ahead of inclusion in August 2018 issue.
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Ploidy level in the genus Leucanthemum correlates with resistance to a specialist herbivore
Ecosphere, 2016Co-Authors: Sonja Stutz, Hariet L. Hinz, Heinz Müller-schärer, Kamil Konowalik, Christoph Oberprieler, Urs SchaffnerAbstract:Polyploidy is considered to be a major source of genetic diversity in plants. Genome duplication has been shown repeatedly to be associated with changes in biotic interactions, but little is known about whether species traits such as herbivore resistance consistently change with increasing ploidy level among closely related plant species. We tested whether larval survival and performance of the specialist root-mining moth Dichrorampha aeratana are influenced by the ploidy level of plant species in the genus Leucanthemum by experimentally infesting 16 different taxa with ploidy levels ranging from diploid to dodecaploid. We found that survival of D. aeratana larvae consistently decreased with increasing ploidy level, irrespective of whether phylogenetic distance among taxa was taken into account or not. The mass of larvae and the proportion of adults emerging from last-instar larvae, however, did not consistently change with increasing ploidy level. Root biomass and dry matter content of the Leucanthemum taxa were neither correlated with ploidy level nor correlated with survival or mass of D. aeratana larvae. In summary, our results provide evidence that in the genus Leucanthemum, resistance to the specialist root herbivore D. aeratana consistently increases with increasing plant ploidy level, but it remains unclear which characteristics associated with polyploidy account for the higher herbivore resistance.
Robert Vogt - One of the best experts on this subject based on the ideXlab platform.
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Karyological and molecular analysis of Leucanthemum (Compositae, Anthemideae) in Corsica
Willdenowia, 2019Co-Authors: Christoph Oberprieler, Florian Wagner, Lena Schinhärl, Laetitia Hugot, Robert VogtAbstract:Karyological, flow-cytometric and molecular analyses indicate that the genus Leucanthemum Mill. (Compositae, Anthemideae) is represented in Corsica (Corse) by two species: the tetraploid L. ircutianum DC. and the hexaploid L. corsicum (Less.) DC. The indication of the occurrence of the diploid L. vulgare Lam. on the island and of a tetraploid chromosome number for L. corsicum, given in former treatments of the genus for Corsica, could not be corroborated. AFLP fingerprinting further suggests that the infraspecific taxonomy of L. corsicum with two subspecies (L. corsicum subsp. corsicum and subsp. fenzlii) and three forms (L. corsicum f. corsicum, f. pinnatifidum and f. eschenlohrianum), which is mainly based on differences in the degree of leaf dissection, is not backed by genetic discontinuities. Owing to the observed little variation in leaf dissection within populations in the wild and the constancy of these features under cultivation, we propose the rank of varieties to taxonomically acknowledge these different stages in the broad spectrum of leaf-dissection grades exhibited by L. corsicum. As a consequence, the two new combinations L. corsicum var. eschenlohrianum (Gamisans) Vogt, Hugot & Oberpr. and L. corsicum var. fenzlii (Gamisans) Vogt, Hugot & Oberpr. are proposed.
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'At the crossroads towards polyploidy': genomic divergence and extent of homoploid hybridization are drivers for the formation of the ox-eye daisy polyploid complex (Leucanthemum, Compositae-Anthemideae).
The New phytologist, 2019Co-Authors: Florian Wagner, Robert Vogt, Tankred Ott, Claudia Zimmer, Verena Reichhart, Christoph OberprielerAbstract:Polyploidy plays a paramount role in phytodiversity, but the causes of this evolutionary pathway require further study. Here, we use phylogenetic methods to examine possible polyploidy-promoting factors by comparing diploid representatives of the comprehensive European polyploid complex Leucanthemum with members of its strictly diploid North African counterpart Rhodanthemum. We investigate genetic divergence and gene flow among all diploid lineages of both genera to evaluate the role of genomic differentiation and hybridization for polyploid speciation. To test whether hybridization in Leucanthemum has been triggered by the geological conditions during its diversification, we additionally generate a time-calibrated phylogeny of 46 species of the subtribe Leucantheminae. Leucanthemum shows a significantly higher genetic divergence and hybridization signal among diploid lineages compared with Rhodanthemum, in spite of a similar crown age and diversification pattern during the Quaternary. Our study demonstrates the importance of genetic differentiation among diploid progenitors and their concurrent affinity for natural hybridization for the formation of a polyploid complex. Furthermore, the role of climate-induced range overlaps on hybridization and polyploid speciation during the Quaternary is discussed.
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Karyological analysis reveals two new polyploid marguerite taxa (Leucanthemum, Compositae–Anthemideae) in S France and NW Italy
Willdenowia, 2018Co-Authors: Robert Vogt, Kamil Konowalik, Christoph OberprielerAbstract:New chromosome counts suggest that the former Leucanthemum vulgare var. esterellense Briq. & Cavill. deserves acknowledgement as an independent species. The new combination L. esterellense (Briq. & Cavill) Vogt, Konowalik & Oberpr., proposed here, denotes a marguerite species endemic to the Massif de l'Esterel (S France) with an octoploid chromosome number of 2n = 72. It is also demonstrated that recently formed hybrids between L. glaucophyllum and L. pallens at Mt Bignone (Liguria, NW Italy) exhibit hepta- and octoploid chromosome numbers. These hybrids are described as L. ×marchii Konowalik, Vogt & Oberpr., a nothospecies new to science. Additionally, the names L. subglaucum de Laramb., L. vulgare var. esterellense Briq. & Cavill. and L. vulgare subsp. glaucophyllum Briq. & Cavill. are lectotypified and new chromosome counts for all presently surveyed Leucanthemum taxa are provided.Citation: Karyological analysis reveals two new polyploid marguerite taxa (Leucanthemum, Compositae–Anthemideae) in S France and NW Italy. – Willdenowia 48: 221–226. doi: https://doi.org/10.3372/wi.48.48204Version of record first published online on 3 August 2018 ahead of inclusion in August 2018 issue.
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karyological analysis reveals two new polyploid marguerite taxa Leucanthemum compositae anthemideae in s france and nw italy
Willdenowia, 2018Co-Authors: Robert Vogt, Kamil Konowalik, Christoph OberprielerAbstract:New chromosome counts suggest that the former Leucanthemum vulgare var. esterellense Briq. & Cavill. deserves acknowledgement as an independent species. The new combination L. esterellense (Briq. & Cavill) Vogt, Konowalik & Oberpr., proposed here, denotes a marguerite species endemic to the Massif de l'Esterel (S France) with an octoploid chromosome number of 2n = 72. It is also demonstrated that recently formed hybrids between L. glaucophyllum and L. pallens at Mt Bignone (Liguria, NW Italy) exhibit hepta- and octoploid chromosome numbers. These hybrids are described as L. ×marchii Konowalik, Vogt & Oberpr., a nothospecies new to science. Additionally, the names L. subglaucum de Laramb., L. vulgare var. esterellense Briq. & Cavill. and L. vulgare subsp. glaucophyllum Briq. & Cavill. are lectotypified and new chromosome counts for all presently surveyed Leucanthemum taxa are provided.Citation: Karyological analysis reveals two new polyploid marguerite taxa (Leucanthemum, Compositae–Anthemideae) in S France and NW Italy. – Willdenowia 48: 221–226. doi: https://doi.org/10.3372/wi.48.48204Version of record first published online on 3 August 2018 ahead of inclusion in August 2018 issue.
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Detecting reticulate relationships among diploid Leucanthemum Mill. (Compositae, Anthemideae) taxa using multilocus species tree reconstruction methods and AFLP fingerprinting.
Molecular phylogenetics and evolution, 2015Co-Authors: Kamil Konowalik, Robert Vogt, Florian Wagner, Salvatore Tomasello, Christoph OberprielerAbstract:Abstract We examined the evolutionary history of the diploid representatives of the genus Leucanthemum Mill. (Compositae, Anthemideae), which constitutes an extensive polyploid complex comprising around 41 species with ploidy levels ranging from 2x to 22x. The inference of phylogenetic relationships even on the diploid level is complicated in this genus due to the overlay of hybridisation and incomplete lineage sorting processes leading to incongruence among gene trees based on nuclear and plastid sequence information. Species tree and network reconstructions were based on gene trees from nine low-copy nuclear markers and the concatenated sequence information for five intergenic spacer regions of the chloroplast genome, either sequenced by Roche 454 pyrosequencing techniques or traditional Sanger sequencing techniques. Additional phylogenetic information came from multi-locus AFLP-fingerprinting of representative individuals of all diploid taxa under study and the subsequent analysis of AFLP patterns with Bayesian clustering and network reconstruction methods. To distinguish between hybridisation and incomplete lineage sorting, we developed and utilized a new ‘hybrid index’ calculation for individual taxa of the data set, which was compared to a simulated null-distribution assuming the occurrence of incomplete lineage sorting alone for pinpointing taxa with a significant hybrid signal. As a result, two species groups with contrasting patterns of gene flow and/or hybrid speciation signals could be identified in the diploids of Leucanthemum: (a) an early-diverging stock of allopatrically distributed diploid species with a lack of evidence for recent hybridisation events among its members and (b) a more recently radiated taxon assemblage with morphologically less clearly circumscribed taxa and a pronounced signal of gene flow among lineages and several candidate taxa, for which a homoploid hybrid origin may be considered.
Kamil Konowalik - One of the best experts on this subject based on the ideXlab platform.
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Karyological analysis reveals two new polyploid marguerite taxa (Leucanthemum, Compositae–Anthemideae) in S France and NW Italy
Willdenowia, 2018Co-Authors: Robert Vogt, Kamil Konowalik, Christoph OberprielerAbstract:New chromosome counts suggest that the former Leucanthemum vulgare var. esterellense Briq. & Cavill. deserves acknowledgement as an independent species. The new combination L. esterellense (Briq. & Cavill) Vogt, Konowalik & Oberpr., proposed here, denotes a marguerite species endemic to the Massif de l'Esterel (S France) with an octoploid chromosome number of 2n = 72. It is also demonstrated that recently formed hybrids between L. glaucophyllum and L. pallens at Mt Bignone (Liguria, NW Italy) exhibit hepta- and octoploid chromosome numbers. These hybrids are described as L. ×marchii Konowalik, Vogt & Oberpr., a nothospecies new to science. Additionally, the names L. subglaucum de Laramb., L. vulgare var. esterellense Briq. & Cavill. and L. vulgare subsp. glaucophyllum Briq. & Cavill. are lectotypified and new chromosome counts for all presently surveyed Leucanthemum taxa are provided.Citation: Karyological analysis reveals two new polyploid marguerite taxa (Leucanthemum, Compositae–Anthemideae) in S France and NW Italy. – Willdenowia 48: 221–226. doi: https://doi.org/10.3372/wi.48.48204Version of record first published online on 3 August 2018 ahead of inclusion in August 2018 issue.
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karyological analysis reveals two new polyploid marguerite taxa Leucanthemum compositae anthemideae in s france and nw italy
Willdenowia, 2018Co-Authors: Robert Vogt, Kamil Konowalik, Christoph OberprielerAbstract:New chromosome counts suggest that the former Leucanthemum vulgare var. esterellense Briq. & Cavill. deserves acknowledgement as an independent species. The new combination L. esterellense (Briq. & Cavill) Vogt, Konowalik & Oberpr., proposed here, denotes a marguerite species endemic to the Massif de l'Esterel (S France) with an octoploid chromosome number of 2n = 72. It is also demonstrated that recently formed hybrids between L. glaucophyllum and L. pallens at Mt Bignone (Liguria, NW Italy) exhibit hepta- and octoploid chromosome numbers. These hybrids are described as L. ×marchii Konowalik, Vogt & Oberpr., a nothospecies new to science. Additionally, the names L. subglaucum de Laramb., L. vulgare var. esterellense Briq. & Cavill. and L. vulgare subsp. glaucophyllum Briq. & Cavill. are lectotypified and new chromosome counts for all presently surveyed Leucanthemum taxa are provided.Citation: Karyological analysis reveals two new polyploid marguerite taxa (Leucanthemum, Compositae–Anthemideae) in S France and NW Italy. – Willdenowia 48: 221–226. doi: https://doi.org/10.3372/wi.48.48204Version of record first published online on 3 August 2018 ahead of inclusion in August 2018 issue.
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Ploidy level in the genus Leucanthemum correlates with resistance to a specialist herbivore
Ecosphere, 2016Co-Authors: Sonja Stutz, Hariet L. Hinz, Heinz Müller-schärer, Kamil Konowalik, Christoph Oberprieler, Urs SchaffnerAbstract:Polyploidy is considered to be a major source of genetic diversity in plants. Genome duplication has been shown repeatedly to be associated with changes in biotic interactions, but little is known about whether species traits such as herbivore resistance consistently change with increasing ploidy level among closely related plant species. We tested whether larval survival and performance of the specialist root-mining moth Dichrorampha aeratana are influenced by the ploidy level of plant species in the genus Leucanthemum by experimentally infesting 16 different taxa with ploidy levels ranging from diploid to dodecaploid. We found that survival of D. aeratana larvae consistently decreased with increasing ploidy level, irrespective of whether phylogenetic distance among taxa was taken into account or not. The mass of larvae and the proportion of adults emerging from last-instar larvae, however, did not consistently change with increasing ploidy level. Root biomass and dry matter content of the Leucanthemum taxa were neither correlated with ploidy level nor correlated with survival or mass of D. aeratana larvae. In summary, our results provide evidence that in the genus Leucanthemum, resistance to the specialist root herbivore D. aeratana consistently increases with increasing plant ploidy level, but it remains unclear which characteristics associated with polyploidy account for the higher herbivore resistance.
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Reconstructing reticulate relationships in the polyploid complex of Leucanthemum Mill. (Compositae, Anthemideae)
2015Co-Authors: Kamil KonowalikAbstract:The genus Leucanthemum consists of ca. 56 taxa occurring mainly in Southern and Central Europe. While there are 19 diploid taxa (2n = 2x = 18) the rest of the genus is formed by an unbroken polyploid chain ranging from tetraploid (2n = 4x = 36) to dokosaploid (2n = 22x = 198). Because of richness in polyploids this genus provides an ideal study system to examine various aspects of polyploidy. This thesis is the starting point to unravel phylogenetic relationships within the whole genus. In this study sampling of all recognized taxa in the genus Leucanthemum is provided with the main aim to reconstruct phylogenetic relationships. The thesis is divided into three chapters with focus on: 1) phylogeny of diploids and homoploid hybrid speciation incidence, 2) phylogeny of all taxa including polyploids, 3) phylogeny and evolutionary processes within L. glaucophyllum group and closely related species. Phylogenies are reconstructed with usage of low-copy nuclear genes sequenced by 454 sequencing accompanied by chloroplast markers. Species tree is constructed in the first Chapter together with species network and hybrid index for all diploid taxa. It reveals that Leucanthemum is monophyletic and that fifteen out of nineteen diploid taxa experienced homoploid hybrid speciation or severe gene flow among species. As a consequence the particular branches in the phylogeny gain rather low support but with robust division of diploids into two groups present in all analyses. In the second Chapter the phylogeny of all taxa is reconstructed as species network which recovers high level of reticulation among them with main finding that taxa from the same geographic area show affinity irrespective of their ploidy level. The third Chapter focuses in more detail on a smaller group of high polyploids related to a decaploid L. glaucophyllum. With reconstruction of species network, nested clade analysis on chloroplast network, and AFLP clustering it brings insights into relationships in this group. It also sheds light on the phylogeography of L. pallens and suggest that gene flow among the same and different ploidy levels is likely occurring. Furthermore new octoploid race is found in Esterell Massif and its relation to other taxa is discussed. The results pinpoint hybridization as one of the major processes in the evolution of the genus Leucanthemum. It influences speciation on the diploid level, enables formation of polyploids and contributes to the reticulate evolution among them in general, and specifically in taxa related to L. glaucophyllum.
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Detecting reticulate relationships among diploid Leucanthemum Mill. (Compositae, Anthemideae) taxa using multilocus species tree reconstruction methods and AFLP fingerprinting.
Molecular phylogenetics and evolution, 2015Co-Authors: Kamil Konowalik, Robert Vogt, Florian Wagner, Salvatore Tomasello, Christoph OberprielerAbstract:Abstract We examined the evolutionary history of the diploid representatives of the genus Leucanthemum Mill. (Compositae, Anthemideae), which constitutes an extensive polyploid complex comprising around 41 species with ploidy levels ranging from 2x to 22x. The inference of phylogenetic relationships even on the diploid level is complicated in this genus due to the overlay of hybridisation and incomplete lineage sorting processes leading to incongruence among gene trees based on nuclear and plastid sequence information. Species tree and network reconstructions were based on gene trees from nine low-copy nuclear markers and the concatenated sequence information for five intergenic spacer regions of the chloroplast genome, either sequenced by Roche 454 pyrosequencing techniques or traditional Sanger sequencing techniques. Additional phylogenetic information came from multi-locus AFLP-fingerprinting of representative individuals of all diploid taxa under study and the subsequent analysis of AFLP patterns with Bayesian clustering and network reconstruction methods. To distinguish between hybridisation and incomplete lineage sorting, we developed and utilized a new ‘hybrid index’ calculation for individual taxa of the data set, which was compared to a simulated null-distribution assuming the occurrence of incomplete lineage sorting alone for pinpointing taxa with a significant hybrid signal. As a result, two species groups with contrasting patterns of gene flow and/or hybrid speciation signals could be identified in the diploids of Leucanthemum: (a) an early-diverging stock of allopatrically distributed diploid species with a lack of evidence for recent hybridisation events among its members and (b) a more recently radiated taxon assemblage with morphologically less clearly circumscribed taxa and a pronounced signal of gene flow among lineages and several candidate taxa, for which a homoploid hybrid origin may be considered.
Maha Refaey - One of the best experts on this subject based on the ideXlab platform.
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quantification of the antibacterial properties of artemisia absinthium a vulgaris chrysanthemum Leucanthemum and achillea millefolium using the vibrio fischeri bacterial bioassay
Acta Botanica Hungarica, 2010Co-Authors: Nora Kovats, Flóra Gölöncsér, Maha RefaeyAbstract:Extended research has been carried out on the antimicrobial properties of herbs belonging to the family Asteraceae, trying to establish their potential use in natural pest control, in addition to human medicinal applications. For testing and quantifying antibacterial activity, most often standard microbial protocols are used. In this study the aggregate bactericide effect of four species ( Artemisia absinthium L., A. vulgaris L., Chrysanthemum Leucanthemum L. and Achillea millefolium L.) was screened using the Vibrio fischeri bioluminescence inhibition bioassay. The two Artemisia species which have well-established use as antibacterial, exerted the highest toxicity. Toxicity of Chrysanthemum Leucanthemum fell into the same range, although this herb has restricted use in human medicine. On the contrary, Achillea millefolium , which is widely recommended against inflammations, showed significantly lower toxicity.
Nora Kovats - One of the best experts on this subject based on the ideXlab platform.
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quantification of the antibacterial properties of artemisia absinthium a vulgaris chrysanthemum Leucanthemum and achillea millefolium using the vibrio fischeri bacterial bioassay
Acta Botanica Hungarica, 2010Co-Authors: Nora Kovats, Flóra Gölöncsér, Maha RefaeyAbstract:Extended research has been carried out on the antimicrobial properties of herbs belonging to the family Asteraceae, trying to establish their potential use in natural pest control, in addition to human medicinal applications. For testing and quantifying antibacterial activity, most often standard microbial protocols are used. In this study the aggregate bactericide effect of four species ( Artemisia absinthium L., A. vulgaris L., Chrysanthemum Leucanthemum L. and Achillea millefolium L.) was screened using the Vibrio fischeri bioluminescence inhibition bioassay. The two Artemisia species which have well-established use as antibacterial, exerted the highest toxicity. Toxicity of Chrysanthemum Leucanthemum fell into the same range, although this herb has restricted use in human medicine. On the contrary, Achillea millefolium , which is widely recommended against inflammations, showed significantly lower toxicity.