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Gautam Sharma - One of the best experts on this subject based on the ideXlab platform.

  • genetic similarity of eggplant shoot and fruit borer Leucinodes orbonalis populations
    DNA and Cell Biology, 2009
    Co-Authors: Murugan Marimuthu, Yasodha Perumal, Abida Puthenpeedikal Salim, Gautam Sharma
    Abstract:

    Shoot and fruit borer, Leucinodes orbonalis (Guenee) (Pyraustidae: Lepidoptera), has become a production constraint in all eggplant (Solanum melongena Linn. [Solanaceae])-growing countries. In India, transgenic eggplants expressing Bacillus thuringiensis Cry toxins have been tested in fields by private- and public-sector agencies. Understanding population diversity is important in designing strategies for better pest management. In the present investigation, random-amplified polymorphic DNA markers were used to assess the genetic diversity of L. orbonalis population collected from different field locations in the Tamilnadu State of India. Of 17 random-amplified polymorphic DNA primers screened, only 11 primers generated polymorphic bands (up to 14 bands). According to their level of similarities, only two major clusters with no variation among population were deduced. Our results indicated that there is a steady genetic flow among the present population of L. orbonalis alleviating genetic variation, which may be attributed to passive and active dispersal of the insect besides absence of host-induced variations among the population. As molecular variability of L. orbonalis population is an important consideration for shoot and fruit damage of the eggplant, constant monitoring is essential to study the possible development of Cry protein resistance in L. orbonalis.

  • Genetic similarity of eggplant shoot and fruit borer, Leucinodes orbonalis, populations.
    DNA and cell biology, 2009
    Co-Authors: Murugan Marimuthu, Yasodha Perumal, Abida Puthenpeedikal Salim, Gautam Sharma
    Abstract:

    Shoot and fruit borer, Leucinodes orbonalis (Guenee) (Pyraustidae: Lepidoptera), has become a production constraint in all eggplant (Solanum melongena Linn. [Solanaceae])–growing countries. In Indi...

Murugan Marimuthu - One of the best experts on this subject based on the ideXlab platform.

  • genetic similarity of eggplant shoot and fruit borer Leucinodes orbonalis populations
    DNA and Cell Biology, 2009
    Co-Authors: Murugan Marimuthu, Yasodha Perumal, Abida Puthenpeedikal Salim, Gautam Sharma
    Abstract:

    Shoot and fruit borer, Leucinodes orbonalis (Guenee) (Pyraustidae: Lepidoptera), has become a production constraint in all eggplant (Solanum melongena Linn. [Solanaceae])-growing countries. In India, transgenic eggplants expressing Bacillus thuringiensis Cry toxins have been tested in fields by private- and public-sector agencies. Understanding population diversity is important in designing strategies for better pest management. In the present investigation, random-amplified polymorphic DNA markers were used to assess the genetic diversity of L. orbonalis population collected from different field locations in the Tamilnadu State of India. Of 17 random-amplified polymorphic DNA primers screened, only 11 primers generated polymorphic bands (up to 14 bands). According to their level of similarities, only two major clusters with no variation among population were deduced. Our results indicated that there is a steady genetic flow among the present population of L. orbonalis alleviating genetic variation, which may be attributed to passive and active dispersal of the insect besides absence of host-induced variations among the population. As molecular variability of L. orbonalis population is an important consideration for shoot and fruit damage of the eggplant, constant monitoring is essential to study the possible development of Cry protein resistance in L. orbonalis.

  • Genetic similarity of eggplant shoot and fruit borer, Leucinodes orbonalis, populations.
    DNA and cell biology, 2009
    Co-Authors: Murugan Marimuthu, Yasodha Perumal, Abida Puthenpeedikal Salim, Gautam Sharma
    Abstract:

    Shoot and fruit borer, Leucinodes orbonalis (Guenee) (Pyraustidae: Lepidoptera), has become a production constraint in all eggplant (Solanum melongena Linn. [Solanaceae])–growing countries. In Indi...

Abida Puthenpeedikal Salim - One of the best experts on this subject based on the ideXlab platform.

  • genetic similarity of eggplant shoot and fruit borer Leucinodes orbonalis populations
    DNA and Cell Biology, 2009
    Co-Authors: Murugan Marimuthu, Yasodha Perumal, Abida Puthenpeedikal Salim, Gautam Sharma
    Abstract:

    Shoot and fruit borer, Leucinodes orbonalis (Guenee) (Pyraustidae: Lepidoptera), has become a production constraint in all eggplant (Solanum melongena Linn. [Solanaceae])-growing countries. In India, transgenic eggplants expressing Bacillus thuringiensis Cry toxins have been tested in fields by private- and public-sector agencies. Understanding population diversity is important in designing strategies for better pest management. In the present investigation, random-amplified polymorphic DNA markers were used to assess the genetic diversity of L. orbonalis population collected from different field locations in the Tamilnadu State of India. Of 17 random-amplified polymorphic DNA primers screened, only 11 primers generated polymorphic bands (up to 14 bands). According to their level of similarities, only two major clusters with no variation among population were deduced. Our results indicated that there is a steady genetic flow among the present population of L. orbonalis alleviating genetic variation, which may be attributed to passive and active dispersal of the insect besides absence of host-induced variations among the population. As molecular variability of L. orbonalis population is an important consideration for shoot and fruit damage of the eggplant, constant monitoring is essential to study the possible development of Cry protein resistance in L. orbonalis.

  • Genetic similarity of eggplant shoot and fruit borer, Leucinodes orbonalis, populations.
    DNA and cell biology, 2009
    Co-Authors: Murugan Marimuthu, Yasodha Perumal, Abida Puthenpeedikal Salim, Gautam Sharma
    Abstract:

    Shoot and fruit borer, Leucinodes orbonalis (Guenee) (Pyraustidae: Lepidoptera), has become a production constraint in all eggplant (Solanum melongena Linn. [Solanaceae])–growing countries. In Indi...

Yasodha Perumal - One of the best experts on this subject based on the ideXlab platform.

  • genetic similarity of eggplant shoot and fruit borer Leucinodes orbonalis populations
    DNA and Cell Biology, 2009
    Co-Authors: Murugan Marimuthu, Yasodha Perumal, Abida Puthenpeedikal Salim, Gautam Sharma
    Abstract:

    Shoot and fruit borer, Leucinodes orbonalis (Guenee) (Pyraustidae: Lepidoptera), has become a production constraint in all eggplant (Solanum melongena Linn. [Solanaceae])-growing countries. In India, transgenic eggplants expressing Bacillus thuringiensis Cry toxins have been tested in fields by private- and public-sector agencies. Understanding population diversity is important in designing strategies for better pest management. In the present investigation, random-amplified polymorphic DNA markers were used to assess the genetic diversity of L. orbonalis population collected from different field locations in the Tamilnadu State of India. Of 17 random-amplified polymorphic DNA primers screened, only 11 primers generated polymorphic bands (up to 14 bands). According to their level of similarities, only two major clusters with no variation among population were deduced. Our results indicated that there is a steady genetic flow among the present population of L. orbonalis alleviating genetic variation, which may be attributed to passive and active dispersal of the insect besides absence of host-induced variations among the population. As molecular variability of L. orbonalis population is an important consideration for shoot and fruit damage of the eggplant, constant monitoring is essential to study the possible development of Cry protein resistance in L. orbonalis.

  • Genetic similarity of eggplant shoot and fruit borer, Leucinodes orbonalis, populations.
    DNA and cell biology, 2009
    Co-Authors: Murugan Marimuthu, Yasodha Perumal, Abida Puthenpeedikal Salim, Gautam Sharma
    Abstract:

    Shoot and fruit borer, Leucinodes orbonalis (Guenee) (Pyraustidae: Lepidoptera), has become a production constraint in all eggplant (Solanum melongena Linn. [Solanaceae])–growing countries. In Indi...

Munusamy Arumugam - One of the best experts on this subject based on the ideXlab platform.

  • Antibacterial and antifeedant activities of Spilanthes acmella leaf extract against Gram-negative and Gram-positive bacteria and brinjal fruit borer, Leucinodes orbonalis larvae
    Journal of Coastal Life Medicine Editorial Office, 2014
    Co-Authors: Manickam Pavunraj, Kathirvelu Baskar, Sundaram Janarthanan, Munusamy Arumugam
    Abstract:

    Objective: To evaluate the antibacterial and antifeedant activities of dichloromethane, acetone and aqueous extracts of Spilanthes acmella (L.) (S. acmella) Murr against selected bacterial strains and larvae of Leucinodes orbonalis Guen. Methods: Solvent extracts were tested against pathogenic microbes using disc diffusion method and fruit disc no-choice method for antifeedant activity. Results: The study revealed that dichloromethane extract of S. acmella leaf showed broad spectrum antibacterial activity against all the tested bacteria. Maximum zone of inhibition was observed in dichloromethane leaf extract of S. acmella against Escherichia coli [(18.9±0.34) mm] followed by Staphylococcus aureus [(18.6±1.31) mm], Proteus vulgaris [(17.2±0.68) mm], Bacillus subtilis [(17.00.76) mm] and Klebsiella pneumonia [(16.4±1.55) mm] at 5 mg/disc concentration. The aqueous extract was moderately inhibited the tested bacteria at all the concentrations. Dichloromethane extract showed good antifeedant activity against Leucinodes orbonalis (68.88%) when compared to acetone (60.80%) and aqueous (45.48%) extracts at 5% concentration. The preliminary phytochemical analysis showed the presence of alkaloids, terpenoids, phytosterols, saponins, steroid, tannins and phenolic compounds. Conclusions: The study suggests that the dichloromethane leaf extract of S. acmella could be used to develop a novel herbal formulation to control pathogenic bacteria and agricultural pests

  • Antibacterial and antifeedant activities of Spilanthes acmella leaf extract against Gram-negative and Gram-positive bacteria and brinjal fruit borer, Leucinodes orbonalis larvae
    2014
    Co-Authors: Manickam Pavunraj, Kathirvelu Baskar, Sundaram Janarthanan, Munusamy Arumugam
    Abstract:

    Objective: To evaluate the antibacterial and antifeedant activities of dichloromethane, acetone and aqueous extracts of Spilanthes acmella (L.) (S. acmella) Murr against selected bacterial strains and larvae of Leucinodes orbonalis Guen. Methods: Solvent extracts were tested against pathogenic microbes using disc diffusion method and fruit disc no-choice method for antifeedant activity. Results: The study revealed that dichloromethane extract of S. acmella leaf showed broad spectrum antibacterial activity against all the tested bacteria. Maximum zone of inhibition was observed in dichloromethane leaf extract of S. acmella against Escherichia coli [(18.9依0.34) mm] followed by Staphylococcus aureus [(18.6依1.31) mm], Proteus vulgaris [(17.2依0.68) mm], Bacillus subtilis [(17.0依0.76) mm] and Klebsiella pneumonia [(16.4依1.55) mm] at 5 mg/disc concentration. The aqueous extract was moderately inhibited the tested bacteria at all the concentrations. Dichloromethane extract showed good antifeedant activity against Leucinodes orbonalis (68.88%) when compared to acetone (60.80%) and aqueous (45.48%) extracts at 5% concentration. The preliminary phytochemical analysis showed the presence of alkaloids, terpenoids, phytosterols, saponins, steroid, tannins and phenolic compounds. Conclusions: The study suggests that the dichloromethane leaf extract of S. acmella could be used to develop a novel herbal formulation to control pathogenic bacteria and agricultural pests. Peer reviewer