The Experts below are selected from a list of 32034 Experts worldwide ranked by ideXlab platform
Daniel Spangler - One of the best experts on this subject based on the ideXlab platform.
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in vitro assessment of chlorhexidine gluconate impregnated polyurethane foam antimicrobial dressing using Zone of Inhibition assays
Infection Control and Hospital Epidemiology, 2004Co-Authors: Shubhangi Bhende, Daniel SpanglerAbstract:Objective:To evaluate an antimicrobial dressing consisting of hydrophilic polyurethane foam with chlorhexidine gluconate for activity against several antibiotic-resistant clinical isolates as well as American Type Culture Collection reference strains using Zone of Inhibition assays.Methods:Sterile foam samples with chlorhexidine gluconate and untreated controls were transferred onto inoculated agar plates. Plates were incubated at 35°C to 37° C for 24 hours and examined for Zones of Inhibition around the foam samples.Results:Polyurethane foam with chlorhexidine gluconate showed antimicrobial activity in vitro against all of the challenge organisms including antibiotic-resistant clinical isolates.Conclusion:The data from this in vitro study support the hypothesis that polyurethane foam with chlorhexidine gluconate has an antimicrobial effect against antibiotic-resistant Staphylococcus and Enterococcus species, as well as Candida species.
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In vitro assessment of chlorhexidine gluconate-impregnated polyurethane foam antimicrobial dressing using Zone of Inhibition assays.
Infection control and hospital epidemiology, 2004Co-Authors: Shubhangi Bhende, Daniel SpanglerAbstract:OBJECTIVE To evaluate an antimicrobial dressing consisting of hydrophilic polyurethane foam with chlorhexidine gluconate for activity against several antibiotic-resistant clinical isolates as well as American Type Culture Collection reference strains using Zone of Inhibition assays. METHODS Sterile foam samples with chlorhexidine gluconate and untreated controls were transferred onto inoculated agar plates. Plates were incubated at 35 degrees C to 37 degrees C for 24 hours and examined for Zones of Inhibition around the foam samples. RESULTS Polyurethane foam with chlorhexidine gluconate showed antimicrobial activity in vitro against all of the challenge organisms including antibiotic-resistant clinical isolates. CONCLUSION The data from this in vitro study support the hypothesis that polyurethane foam with chlorhexidine gluconate has an antimicrobial effect against antibiotic-resistant Staphylococcus and Enterococcus species, as well as Candida species.
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In vitro antimicrobial evaluation of Coated VICRYL* Plus Antibacterial Suture (coated polyglactin 910 with triclosan) using Zone of Inhibition assays.
Surgical infections, 2002Co-Authors: Stephen Rothenburger, Shubhangi Bhende, Daniel Spangler, Daniel BurkleyAbstract:In this study, coated polyglactin 910 suture with triclosan was evaluated for its ability to inhibit the growth of wild-type and methicillin-resistant Staphylococcus aureus and S. epidermidis using several in vitro models. In the standard experimental procedure, sterile sutures were inoculated with bacteria, plated in semisolid culture media, incubated for 24 h, and examined for Zones of Inhibition surrounding the sutures. Sutures were pretreated by several methods prior to inoculation, these included: 24-h aqueous immersion, serial 7-day aqueous immersion, knotting, and passage through subcutaneous and fascial tissue. Sutures were also compared to determine if suture diameter had an effect on the Zone of Inhibition. Coated polyglactin 910 suture with triclosan inhibited growth of test organisms consistently over a range of suture diameters and treatment conditions. The antibacterial effect was robust and did not diminish when sutures were placed in an aqueous environment for up to 7 days. Knotted suture with triclosan inhibited bacterial colonization after replicate inoculation. The bacteria-free Zone surrounding each knotted suture had a volume of 14.5 cm(3) for S. epidermidis and 17.8 cm(3) for S. aureus. Coated polyglactin 910 suture with triclosan continued to produce Zones of Inhibition after five and 10 passes through fascia and subcutaneous tissue. These data support the conclusion that coated polyglactin 910 suture with triclosan provides antimicrobial effect sufficient to prevent in vitro colonization by S. aureus and S. epidermidis.
Shubhangi Bhende - One of the best experts on this subject based on the ideXlab platform.
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in vitro assessment of chlorhexidine gluconate impregnated polyurethane foam antimicrobial dressing using Zone of Inhibition assays
Infection Control and Hospital Epidemiology, 2004Co-Authors: Shubhangi Bhende, Daniel SpanglerAbstract:Objective:To evaluate an antimicrobial dressing consisting of hydrophilic polyurethane foam with chlorhexidine gluconate for activity against several antibiotic-resistant clinical isolates as well as American Type Culture Collection reference strains using Zone of Inhibition assays.Methods:Sterile foam samples with chlorhexidine gluconate and untreated controls were transferred onto inoculated agar plates. Plates were incubated at 35°C to 37° C for 24 hours and examined for Zones of Inhibition around the foam samples.Results:Polyurethane foam with chlorhexidine gluconate showed antimicrobial activity in vitro against all of the challenge organisms including antibiotic-resistant clinical isolates.Conclusion:The data from this in vitro study support the hypothesis that polyurethane foam with chlorhexidine gluconate has an antimicrobial effect against antibiotic-resistant Staphylococcus and Enterococcus species, as well as Candida species.
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In vitro assessment of chlorhexidine gluconate-impregnated polyurethane foam antimicrobial dressing using Zone of Inhibition assays.
Infection control and hospital epidemiology, 2004Co-Authors: Shubhangi Bhende, Daniel SpanglerAbstract:OBJECTIVE To evaluate an antimicrobial dressing consisting of hydrophilic polyurethane foam with chlorhexidine gluconate for activity against several antibiotic-resistant clinical isolates as well as American Type Culture Collection reference strains using Zone of Inhibition assays. METHODS Sterile foam samples with chlorhexidine gluconate and untreated controls were transferred onto inoculated agar plates. Plates were incubated at 35 degrees C to 37 degrees C for 24 hours and examined for Zones of Inhibition around the foam samples. RESULTS Polyurethane foam with chlorhexidine gluconate showed antimicrobial activity in vitro against all of the challenge organisms including antibiotic-resistant clinical isolates. CONCLUSION The data from this in vitro study support the hypothesis that polyurethane foam with chlorhexidine gluconate has an antimicrobial effect against antibiotic-resistant Staphylococcus and Enterococcus species, as well as Candida species.
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In vitro antimicrobial evaluation of Coated VICRYL* Plus Antibacterial Suture (coated polyglactin 910 with triclosan) using Zone of Inhibition assays.
Surgical infections, 2002Co-Authors: Stephen Rothenburger, Shubhangi Bhende, Daniel Spangler, Daniel BurkleyAbstract:In this study, coated polyglactin 910 suture with triclosan was evaluated for its ability to inhibit the growth of wild-type and methicillin-resistant Staphylococcus aureus and S. epidermidis using several in vitro models. In the standard experimental procedure, sterile sutures were inoculated with bacteria, plated in semisolid culture media, incubated for 24 h, and examined for Zones of Inhibition surrounding the sutures. Sutures were pretreated by several methods prior to inoculation, these included: 24-h aqueous immersion, serial 7-day aqueous immersion, knotting, and passage through subcutaneous and fascial tissue. Sutures were also compared to determine if suture diameter had an effect on the Zone of Inhibition. Coated polyglactin 910 suture with triclosan inhibited growth of test organisms consistently over a range of suture diameters and treatment conditions. The antibacterial effect was robust and did not diminish when sutures were placed in an aqueous environment for up to 7 days. Knotted suture with triclosan inhibited bacterial colonization after replicate inoculation. The bacteria-free Zone surrounding each knotted suture had a volume of 14.5 cm(3) for S. epidermidis and 17.8 cm(3) for S. aureus. Coated polyglactin 910 suture with triclosan continued to produce Zones of Inhibition after five and 10 passes through fascia and subcutaneous tissue. These data support the conclusion that coated polyglactin 910 suture with triclosan provides antimicrobial effect sufficient to prevent in vitro colonization by S. aureus and S. epidermidis.
Ross Barnard - One of the best experts on this subject based on the ideXlab platform.
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the Zone of Inhibition
Clinical Chemistry, 2019Co-Authors: Ross BarnardAbstract:Some old methods are still in use because they are simple and effective. One such method is the disk diffusion (or agar diffusion) test (1, 2). The diameter of the clear Zone around an antibiotic-paper disk enables us to directly, and elegantly, visualize the inhibitory potency of an antibiotic. This test is …
Preena Sidhu - One of the best experts on this subject based on the ideXlab platform.
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assessment of antimicrobial activity of different concentrations of tinospora cordifolia against streptococcus mutans an in vitro study
Dental research journal, 2019Co-Authors: Sankalp Agarwal, Priyadarshini Hesarghatta Ramamurthy, Bennete Fernandes, Avita Rath, Preena SidhuAbstract:Background: The antimicrobial property of Tinospora cordifolia has been tested against a variety of microorganisms in the literature. The present study aimed to assess the antimicrobial activity of different concentrations of commercially available T. cordifolia powder against Streptococcus mutans . Materials and Methods: An in vitro study was undertaken in which extract of T. cordifolia was obtained using 100% ethanol by maceration. Seven different concentrations were prepared and tested against S. mutans in brain–heart infusion agar medium. Plates were incubated aerobically at 37°C for 48 h, and Zone of Inhibition was measured using Vernier caliper. 0.2% chlorhexidine and dimethylformamide were used as positive and negative controls respectively. The data were analysed by descriptive analytic tests Results: The maximum antibacterial activity of T. cordifolia was observed with a volume of 40 μl at 2% concentration with a Zone of Inhibition of 19 mm. A 30 μl volume of 0.2% chlorhexidine showed a Zone of Inhibition of 28 mm, and no Zone of Inhibition was observed with dimethylformamide. Conclusion: Tinospora exhibited antimicrobial activity against S. mutans . However, it needs to be confirmed further with in vivo studies. Key Words: Antimicrobial, Streptococcus mutans , Tinospora
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Assessment of antimicrobial activity of different concentrations of Tinospora cordifolia against Streptococcus mutans: An in vitro study
Wolters Kluwer Medknow Publications, 2019Co-Authors: Sankalp Agarwal, Priyadarshini Hesarghatta Ramamurthy, Bennete Fernandes, Avita Rath, Preena SidhuAbstract:Background: The antimicrobial property of Tinospora cordifolia has been tested against a variety of microorganisms in the literature. The present study aimed to assess the antimicrobial activity of different concentrations of commercially available T. cordifolia powder against Streptococcus mutans. Materials and Methods: An in vitro study was undertaken in which extract of T. cordifolia was obtained using 100% ethanol by maceration. Seven different concentrations were prepared and tested against S. mutans in brain–heart infusion agar medium. Plates were incubated aerobically at 37°C for 48 h, and Zone of Inhibition was measured using Vernier caliper. 0.2% chlorhexidine and dimethylformamide were used as positive and negative controls respectively. The data were analysed by descriptive analytic tests. Results: The maximum antibacterial activity of T. cordifolia was observed with a volume of 40 μl at 2% concentration with a Zone of Inhibition of 19 mm. A 30 μl volume of 0.2% chlorhexidine showed a Zone of Inhibition of 28 mm, and no Zone of Inhibition was observed with dimethylformamide. Conclusion: Tinospora exhibited antimicrobial activity against S. mutans. However, it needs to be confirmed further with in vivo studies
Lokendra Singh - One of the best experts on this subject based on the ideXlab platform.
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Research Article Bioactivity of Root Endophytic Freshwater Hyphomycetes
2016Co-Authors: Anguillospora Longissima, S. C. Sati, Syd Ingold, Lokendra SinghAbstract:License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Anguillospora longissima, isolated from root as endophytic freshwater hyphomycetes, was evaluated for its bioactivity (antibacterial potential) against five bacterial strains, namely, Gram-positive (Bacillus subtilis MTCC 121) and Gram-negative (Agrobacterium tumefaciens MTCC 609, Escherichia coli MTCC 40, Erwinia chrysanthemum, and Xanthomonas pseudomonas). Antimicrobial activity was assessed by measuring the Zone of Inhibition with preliminary and secondary antimicrobial assays.The applied fungus was found significant for all tested bacterial strains as showen by their Zone of Inhibition. In preliminary antimicrobial assay, maximum Zone of Inhibition was recorded against Gram-negative human pathogenic bacterial strain Escherichia coli (23mm) followed by Erwinia chrysanthemi (22mm), Agrobacterium tumefaciens (21mm), and Xanthomonas phaseoli (21mm), while minimum Zone of Inhibition was observed against Bacillus subtilis (20mm). In secondary antimicrobial assay, the maximum Zone of Inhibition was recorded against Erwinia chrysanthemi (11mm) followed by Agrobacterium tumefaciens (10mm), Xanthomonas phaseoli (10mm), and Bacillus subtilis (9mm) and minimum Inhibition was found against Escherichia coli (8mm). 1
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Bioactivity of root endophytic freshwater Hyphomycetes Anguillospora longissima (Sacc. & Syd.) Ingold.
TheScientificWorldJournal, 2014Co-Authors: S. C. Sati, Lokendra SinghAbstract:Anguillospora longissima, isolated from root as endophytic freshwater hyphomycetes, was evaluated for its bioactivity (antibacterial potential) against five bacterial strains, namely, Gram-positive (Bacillus subtilis MTCC 121) and Gram-negative (Agrobacterium tumefaciens MTCC 609, Escherichia coli MTCC 40, Erwinia chrysanthemum, and Xanthomonas pseudomonas). Antimicrobial activity was assessed by measuring the Zone of Inhibition with preliminary and secondary antimicrobial assays. The applied fungus was found significant for all tested bacterial strains as showen by their Zone of Inhibition. In preliminary antimicrobial assay, maximum Zone of Inhibition was recorded against Gram-negative human pathogenic bacterial strain Escherichia coli (23 mm) followed by Erwinia chrysanthemi (22 mm), Agrobacterium tumefaciens (21 mm), and Xanthomonas phaseoli (21 mm), while minimum Zone of Inhibition was observed against Bacillus subtilis (20 mm). In secondary antimicrobial assay, the maximum Zone of Inhibition was recorded against Erwinia chrysanthemi (11 mm) followed by Agrobacterium tumefaciens (10 mm), Xanthomonas phaseoli (10 mm), and Bacillus subtilis (9 mm) and minimum Inhibition was found against Escherichia coli (8 mm).
