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J Oros - One of the best experts on this subject based on the ideXlab platform.

  • comparative study of hematologic and plasma biochemical variables in eastern atlantic juvenile and adult nesting loggerhead sea turtles caretta caretta
    Veterinary Clinical Pathology, 2009
    Co-Authors: Ana B Casal, Maria Camacho, Luis Felipe Lopezjurado, Candelaria Juste, J Oros
    Abstract:

    Background: Plasma biochemical and hematologic variables are important in the management of endangered sea turtles, such as loggerheads. However, studies on blood biochemistry and hematology of loggerheads are limited, and different concentrations according to variable criteria have been reported. Objective: The purpose of this study was to establish and compare baseline plasma chemistry and hematology values in Eastern Atlantic juvenile and adult nesting loggerhead sea turtles (Caretta caretta). Methods: Blood samples were collected from 69 healthy juvenile loggerhead sea turtles after their rehabilitation in captivity, and from 34 adult nesting loggerheads after oviposition. Fresh blood was used for Leukocyte Differential Count and PCV determination. Heparinized blood was used for RBC and WBC Counts. Plasma biochemical concentrations were measured using an automated biochemical analyzer. For the comparative study, nonparametric statistical analysis was done using the Mann–Whitney U-test. Results: Minimum, maximum, and median concentrations were obtained for 14 hematologic and 15 plasma chemistry variables. Statistically significant differences between juvenile and adult turtles were found for PCV; RBC, WBC, and Leukocyte Differential Counts; total protein, albumin, globulins, calcium, triglycerides, glucose, total cholesterol and urea concentrations; and lactate dehydrogenase activity. Conclusions: Age, size, and reproductive status cause important variations in the hematologic and plasma biochemical results of loggerheads. The reference values obtained in this study may be used as a standard profile, useful for veterinary surgeons involved in sea turtle conservation.

  • morphologic and cytochemical characteristics of blood cells of juvenile loggerhead sea turtles caretta caretta
    Research in Veterinary Science, 2007
    Co-Authors: A B Casal, J Oros
    Abstract:

    A morphologic classification based on the cytochemical characteristics of blood cells of 35 juvenile loggerhead sea turtles (Caretta caretta) is described. Cytochemical stains included benzidine peroxidase, chloroacetate esterase, alpha-naphthyl butyrate esterase (with and without sodium fluoride), acid phosphatase (with and without tartaric acid), Sudan black B, periodic acid-Schiff, and toluidine blue. The morphologic characteristics of erythrocytes were similar to those reported in green turtles. Six types of white blood cells were identified: heterophils, eosinophils, basophils, lymphocytes, monocytes and thrombocytes. Except for the basophils, the rest of the white blood cells from loggerhead turtles had different cytochemical characteristics compared to blood cells from other sea turtle species. The Leukocyte Differential Count was different from that reported for other sea turtle species. Heterophils were the most numerous Leukocytes from these loggerhead turtles, followed by lymphocytes, eosinophils, monocytes and basophils. This paper provides a morphologic classification of blood cells of loggerhead sea turtles that is useful for veterinary surgeons involved in sea turtle conservation.

Mauro Buttarello - One of the best experts on this subject based on the ideXlab platform.

  • automated blood cell Counts state of the art
    American Journal of Clinical Pathology, 2008
    Co-Authors: Mauro Buttarello, Mario Plebani
    Abstract:

    The CBC Count and Leukocyte Differential Count (LDC) are among the most frequently requested clinical laboratory tests. These analyses are highly automated, and the correct interpretation of results requires extensive knowledge of the analytic performance of the instruments and the clinical significance of the results they provide. In this review, we analyze the state of the art regarding traditional and new parameters with emphasis on clinical applications and analytic quality. The problems of some traditional parameters of the CBC Count, such as platelet Counts, some components of the LDC such as monocyte and basophil Counts, and other commonly used indices such as red cell volume distribution width and platelet indices such as mean platelet volume and platelet distribution width are considered. The new parameters, evaluated from analytic and clinical viewpoints, are the available components of the extended Differential Count (hematopoietic progenitor cells, immature granulocytes, and erythroblasts), the immature reticulocyte fraction, the reticulocyte indices, the fragmented RBCs, and the immature platelet fraction. During the last 2 decades, automated blood cell Counters have undergone a formidable technological evolution owing to the introduction of new physical principles for cellular analysis and the progressive evolution of software. The results have been an improvement in analytic efficiency and an increase in information provided, which, however, require ever more specialized knowledge to best discern the possible clinical applications. In addition to the traditional parameters of the CBC Count and Leukocyte Differential Count (LDC), the more complete analyzers are able to provide much more information, both quantitative, such as the extended Differential Count (EDC), and qualitative. The latter is represented by flags that indicate technical problems (eg, malfunction, analytic interference) and, above all, cells that are normally absent from peripheral blood such as blasts, atypical lymphocytes, immature granulocytes (IGs), and nucleated RBCs (NRBCs). For some consolidated parameters, such as WBC and RBC Counts, hemoglobin concentration, or mean corpuscular volume (MCV), analytic performance is generally excellent. 1 For others, in particular, certain components of the LDC and reticulocyte or platelet Counts, especially at low concentrations, performance is less satisfactory. 2,3 Further considerations are necessary regarding the possible clinical use of new analytic parameters that are available only with automated analyzers but that have not yet reached their full potential. The immature reticulocyte fraction (IRF), reticulocyte indices such as mean reticulocyte volume (MCVr) and mean reticulocyte hemoglobin content (CHr), fragmented RBC (FRBC) Count, and the immature platelet fraction (IPF) are among these. Other parameters such as the RBC distribution width (RDW) and platelet indices, such as the mean platelet volume

  • automated blood cell Counts state of the art
    American Journal of Clinical Pathology, 2008
    Co-Authors: Mauro Buttarello, Mario Plebani
    Abstract:

    The CBC Count and Leukocyte Differential Count (LDC) are among the most frequently requested clinical laboratory tests. These analyses are highly automated, and the correct interpretation of results requires extensive knowledge of the analytic performance of the instruments and the clinical significance of the results they provide. In this review, we analyze the state of the art regarding traditional and new parameters with emphasis on clinical applications and analytic quality. The problems of some traditional parameters of the CBC Count, such as platelet Counts, some components of the LDC such as monocyte and basophil Counts, and other commonly used indices such as red cell volume distribution width and platelet indices such as mean platelet volume and platelet distribution width are considered. The new parameters, evaluated from analytic and clinical viewpoints, are the available components of the extended Differential Count (hematopoietic progenitor cells, immature granulocytes, and erythroblasts), the immature reticulocyte fraction, the reticulocyte indices, the fragmented RBCs, and the immature platelet fraction.

  • diagnostic performance a comparative study of the Leukocyte Differential Count on four automated haematology analysers
    Clinical Chemistry and Laboratory Medicine, 1993
    Co-Authors: Mauro Buttarello, C Lorenz, Miralda Gadotti, Emanuela Toffalori, A Valentini, Paolo Rizzotti
    Abstract:

    Summary: The diagnostic performance of the haematology analysers, Technicon Hl, Sysmex NE 8000, Coulter STKS and Sequoia Cell Dyn 3000 was determined by comparing their results for automated Differential Counting with the result obtained for 400 cells by the manual microscopic method. These comparative studies were performed on a group of adults, containing both normal individuals (n = 150) and those affected by various pathologies (n = 113). The number of morphologically false negatives is low for all the systems (from 1.8% for the Cell Dyn 3000 to 6.2% for the NE 8000), while the number of distributionally false negatives was slightly higher (from 4.4% for the Cell Dyn 3000 to 7% for the HI and NE 8000). The morphological flags, though useful for improving the diagnostic performance of the instruments, show a rather modest sensitivity when taken individually: immature granulocytes/bands from 71% for the STKS to 43% for the NE 8000; blasts from 66.6% for the HI to 53.3% for the STKS, atypical lymphocytes from 59% for the HI to 13.6% for the NE 8000; erythroblasts from 42.8% for the STKS to 7% for the NE 8000.

A B Casal - One of the best experts on this subject based on the ideXlab platform.

  • morphologic and cytochemical characteristics of blood cells of juvenile loggerhead sea turtles caretta caretta
    Research in Veterinary Science, 2007
    Co-Authors: A B Casal, J Oros
    Abstract:

    A morphologic classification based on the cytochemical characteristics of blood cells of 35 juvenile loggerhead sea turtles (Caretta caretta) is described. Cytochemical stains included benzidine peroxidase, chloroacetate esterase, alpha-naphthyl butyrate esterase (with and without sodium fluoride), acid phosphatase (with and without tartaric acid), Sudan black B, periodic acid-Schiff, and toluidine blue. The morphologic characteristics of erythrocytes were similar to those reported in green turtles. Six types of white blood cells were identified: heterophils, eosinophils, basophils, lymphocytes, monocytes and thrombocytes. Except for the basophils, the rest of the white blood cells from loggerhead turtles had different cytochemical characteristics compared to blood cells from other sea turtle species. The Leukocyte Differential Count was different from that reported for other sea turtle species. Heterophils were the most numerous Leukocytes from these loggerhead turtles, followed by lymphocytes, eosinophils, monocytes and basophils. This paper provides a morphologic classification of blood cells of loggerhead sea turtles that is useful for veterinary surgeons involved in sea turtle conservation.

Mario Plebani - One of the best experts on this subject based on the ideXlab platform.

  • automated blood cell Counts state of the art
    American Journal of Clinical Pathology, 2008
    Co-Authors: Mauro Buttarello, Mario Plebani
    Abstract:

    The CBC Count and Leukocyte Differential Count (LDC) are among the most frequently requested clinical laboratory tests. These analyses are highly automated, and the correct interpretation of results requires extensive knowledge of the analytic performance of the instruments and the clinical significance of the results they provide. In this review, we analyze the state of the art regarding traditional and new parameters with emphasis on clinical applications and analytic quality. The problems of some traditional parameters of the CBC Count, such as platelet Counts, some components of the LDC such as monocyte and basophil Counts, and other commonly used indices such as red cell volume distribution width and platelet indices such as mean platelet volume and platelet distribution width are considered. The new parameters, evaluated from analytic and clinical viewpoints, are the available components of the extended Differential Count (hematopoietic progenitor cells, immature granulocytes, and erythroblasts), the immature reticulocyte fraction, the reticulocyte indices, the fragmented RBCs, and the immature platelet fraction. During the last 2 decades, automated blood cell Counters have undergone a formidable technological evolution owing to the introduction of new physical principles for cellular analysis and the progressive evolution of software. The results have been an improvement in analytic efficiency and an increase in information provided, which, however, require ever more specialized knowledge to best discern the possible clinical applications. In addition to the traditional parameters of the CBC Count and Leukocyte Differential Count (LDC), the more complete analyzers are able to provide much more information, both quantitative, such as the extended Differential Count (EDC), and qualitative. The latter is represented by flags that indicate technical problems (eg, malfunction, analytic interference) and, above all, cells that are normally absent from peripheral blood such as blasts, atypical lymphocytes, immature granulocytes (IGs), and nucleated RBCs (NRBCs). For some consolidated parameters, such as WBC and RBC Counts, hemoglobin concentration, or mean corpuscular volume (MCV), analytic performance is generally excellent. 1 For others, in particular, certain components of the LDC and reticulocyte or platelet Counts, especially at low concentrations, performance is less satisfactory. 2,3 Further considerations are necessary regarding the possible clinical use of new analytic parameters that are available only with automated analyzers but that have not yet reached their full potential. The immature reticulocyte fraction (IRF), reticulocyte indices such as mean reticulocyte volume (MCVr) and mean reticulocyte hemoglobin content (CHr), fragmented RBC (FRBC) Count, and the immature platelet fraction (IPF) are among these. Other parameters such as the RBC distribution width (RDW) and platelet indices, such as the mean platelet volume

  • automated blood cell Counts state of the art
    American Journal of Clinical Pathology, 2008
    Co-Authors: Mauro Buttarello, Mario Plebani
    Abstract:

    The CBC Count and Leukocyte Differential Count (LDC) are among the most frequently requested clinical laboratory tests. These analyses are highly automated, and the correct interpretation of results requires extensive knowledge of the analytic performance of the instruments and the clinical significance of the results they provide. In this review, we analyze the state of the art regarding traditional and new parameters with emphasis on clinical applications and analytic quality. The problems of some traditional parameters of the CBC Count, such as platelet Counts, some components of the LDC such as monocyte and basophil Counts, and other commonly used indices such as red cell volume distribution width and platelet indices such as mean platelet volume and platelet distribution width are considered. The new parameters, evaluated from analytic and clinical viewpoints, are the available components of the extended Differential Count (hematopoietic progenitor cells, immature granulocytes, and erythroblasts), the immature reticulocyte fraction, the reticulocyte indices, the fragmented RBCs, and the immature platelet fraction.

Hyunsook Chi - One of the best experts on this subject based on the ideXlab platform.

  • an extended Leukocyte Differential Count 16 types of circulating Leukocytes using the cytodiff flow cytometric system can provide information for the discrimination of sepsis severity and prediction of outcome in sepsis patients
    Cytometry Part B-clinical Cytometry, 2013
    Co-Authors: Sang Hyuk Park, Borae G Park, Chanjeoung Park, Seongsoo Jang, Sue Kim, Duckhee Kim, Sukkyung Hong, Hyunsook Chi
    Abstract:

    Background The Beckman Coulter CytoDiff flow cytometric system (Beckman Coulter, Miami, FL) was recently developed for performing Leukocyte Differential Counts in up to 16 Leukocyte subpopulations. We compared these Leukocyte subpopulation levels among patients with three stages of sepsis (uncomplicated sepsis, severe sepsis, septic shock), especially focused on the discrimination of complicated sepsis from uncomplicated sepsis. Methods We examined a total of 181 samples with sepsis who were admitted to the surgical intensive care unit. In addition, we examined samples obtained from 60 normal healthy volunteers. Both the proportions and absolute numbers of each cell type in the four groups were obtained using the CytoDiff flow cytometric system and compared. Results Mature neutrophils and immature granulocytes failed to discriminate patients with complicated sepsis from those with uncomplicated sepsis although their absolute numbers were increased compared with normal controls. In contrast, almost all lymphocyte subpopulations and CD16(–) monocytes decreased significantly in patients with complicated sepsis compared with uncomplicated sepsis. Among them, only B lymphocytes showed independent ability to discriminate two groups. Both B lymphocytes and CD16(–) monocytes possessed a significant adverse prognostic impact on overall survival when their absolute numbers decreased. Conclusions Almost all lymphocyte subpopulations and CD16(–) monocytes decrease in size with increasing sepsis severity. Among them, only B lymphocytes showed independent ability to discriminate patients with complicated sepsis from those with uncomplicated sepsis. Both B lymphocytes and CD16 (–) monocytes show a significant adverse prognostic impact on overall survival outcomes in sepsis patients when their absolute numbers are decreased. © 2013 International Clinical Cytometry Society

  • the extended Leukocyte Differential Count using the cytodiff flow cytometric system reveals that higher cd16 cytotoxic nk t lymphocyte levels predict superior survival outcomes in patients with metastatic carcinoma
    Cytometry Part B-clinical Cytometry, 2013
    Co-Authors: Borae G Park, Chanjeoung Park, Chanhee Yoon, Seongsoo Jang, Hyunsook Chi, Minhee Ryu, Sangwe Kim
    Abstract:

    The recently developed Cytodiff flow cytometric system (Beckman Coulter, Miami, FL) enables Leukocyte analysis using a single immunophenotyping panel tube composed of six markers and five colors and that can detect 16 Leukocyte subpopulations. We performed a preliminary investigation of whether changes in any of 16 Leukocyte Differentials were associated with survival and treatment outcomes in patients with metastatic carcinoma or not. We measured 16 Leukocyte Differential Counts using the Cytodiff flow cytometric system in peripheral blood samples from 40 patients with metastatic malignancy (27 stomach cancer and 13 lung cancer) before chemotherapy and at 15 day intervals after chemotherapy for 2 months. A higher percentage of CD16+ cytotoxic NK+T lymphocytes was found to be the only significant prognostic factor among by Cox regression analysis and a higher percentage of CD16+ cytotoxic NK+T lymphocytes (>5.0%) showed significantly longer survival outcomes by Kaplan-Meier analysis (P = 0.003). The Cytodiff system enables 16 Leukocyte subpopulations in a one tube assay and also can operate with only small amounts of sample, although it cannot differentiate NK cells from T lymphocytes. Hence, the monitoring of all Leukocyte subpopulations using Cytodiff flow cytometry may be a helpful prognostic tool for patients with metastatic carcinoma.