Trametes

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Méndez Gabriela - One of the best experts on this subject based on the ideXlab platform.

  • BIODEGRADACIÓN DE LOS FENOLES PRESENTES EN EL EXTRACTO DE GUARANGO(Caesalpinia Spinosa) Y EN LOS COLIRANTES ÍNDIGO CARMÍN, NARANJA II Y ROJO FENOL A TRAVÉZ DE Trametes Versicolor AND Aspergillus Niger.
    2014
    Co-Authors: Huachi Laura, Macas, Ángela Macas, Méndez Gabriela
    Abstract:

    Esta investigación se la realizó con el fin de evaluar la capacidad de biodegradación de los fenoles presentes en el extracto de tánico de guarango((Caesalpina spinosa) y en los colorantes índigo carmín, naranja II y el rojo fenol, presentes en los desechos de la industria textil. Se utilizaron dos tipos de hongos, el de podredumbr blanca Trametes versicolor y filamentos Aspergillus niger : Los tratamientos bológicos se aplicaron por triplicado, reaoizados por el método expectrofotométrico Uv por 15 días; los resultados obtenidos al aplicar el hongo Trametes versicolor en el extracto titáico fue del 69.45% de degradación; y en el colorante índigo carmín fue del 100% de decoloración. Los resultados de la degradación de Trametes versicolor y Aspergillus niger sobre el colorante rojo fenol se obtuvieron porcentajes de decoloración no mayores al 26%. Los hongos aplicados en los tratamientos biológicos presentaron tolerancia tanto a la composición del extracto tánico como a las concentraciones de cada uno de los colorantes usados, esto fue comprobado con la cinética de crecimiento para cada hongo.The research was done to evaluate phenol biodregadation capacity in Guarango(Caesalpina spinosa) tannic extract phenols and in indigo carmine, orange II and red phenols, common residues in the textile industry. Two types of fungi were used, white-rot Trametes versicolor fungus and the filamentous Aspergillus niger. Biological treatments were applied in triplicate trough the spectrophotometric UV method for 15 days. When applying the Trametes versicolor fungis to the tannic extract, there was 69.45% degradation, and a 100% discoloration in the indigo carmine. When applying Aspergillus niger fungus to the tannic extract and orange II dye, there was a 63.45% and 32.78% degradatión, respectively. There was a discoloration no greater than 26% when applying Trametes versicolor and Aspergillus niger to the red phenol. The fungi applied during the biological treatement presented tolerance to both the tannic extract composition as well as the concentration of each dye used, verified through growing kinetics of each fungu

  • GUARANGO EXTRACT (Caesalpinia Spinosa) AND INDIGO CARMINE, ORANGE II, AND RED DYES PHENOL BIODEGRADATION THROUGH Trametes Versicolor AND Aspergillus Niger
    'Salesian Polytechnic University of Ecuador', 2014
    Co-Authors: Huachi Laura, Macas Ángela, Méndez Gabriela
    Abstract:

    Esta investigación se la realizó con el fin de evaluar la capacidad de biodegradación de los fenoles presentes en el extracto de tánico de guarango (Caesalpinia spinosa) y en los colorantes índigo carmín, naranja II y rojo fenol, presentes en los desechos de la industria textil. Se utilizaron dos tipos de hongos, el de podredumbre blanca Trametes versicolor y filamentoso Aspergillus niger. Los tratamientos biológicos se aplicaron por triplicado, realizados por el método espectrofotométrico UV por 15 días; los resultados obtenidos al aplicar el hongo Trametes versicolor en el extracto tánico fue del 69,45 % de degradación; y en el colorante índigo carmín fue del 100 % de decoloración. Los resultados de la degradación con el hongo Aspergillus niger sobre el extracto tánico fue del 63,45 % y en el colorante naranja II fue del 32,78 %. En la aplicación de Trametes versicolor y Aspergillus niger sobre el colorante rojo fenol se obtuvieron porcentajes de decoloración no mayores al 26 %. Los hongos aplicados en los tratamientos biológicos presentaron tolerancia tanto a la composición del extracto tánico como a las concentraciones de cada uno de los colorantes usados, esto fue comprobado con la cinética de crecimiento para cada hongo.This research was done to evaluate phenol biodegradation capacity in Guarango (Caesalpinia spinosa) tannic extract phenols and in indigo carmine, orange II and red phenols, common residues in the textile industry. Two types of fungi were used, white-rot Trametes versicolor fungus and the filamentous Aspergillus niger. Biological treatments were applied in triplicate through the spectrophotometric UV method for 15 days. When applying the Trametes versicolor fungus to the tannic extract, there was 69.45 % degradation, and a 100 % discoloration in the indigo carmine. When applying the Aspergillus niger fungus to the tannic extract and orange II dye, there was a 63,45 % and 32,78 % degradation, respectively. There was a discoloration no greater than 26 % when applying Trametes versicolor and Aspergillus niger to the red phenol. The fungi applied during the biological treatment presented tolerance to both the tannic extract composition as well as the concentration of each dye used, verified through growing kinetics of each fungus

  • Biodegradación de los fenoles presentes en el extracto de guarango (caesalpinia spinosa) y en los colorantes índigo carmín, naranja ii y rojo fenol a través de Trametes versicolor y aspergillus niger
    2014
    Co-Authors: Huachi Laura, Macías Ángel, Méndez Gabriela
    Abstract:

    This research was done to evaluate phenol biodegradation capacity in Guarango (Caesalpinia spinosa) tannic extract phenols and in indigo carmine, orange II and red phenols, common residues in the textile industry. Two types of fungi were used, white-rot Trametes versicolor fungus and the filamentous Aspergillus niger. Biological treatments were applied in triplicate through the spectrophotometric UV method for 15 days. When applying the Trametes versicolor fungus to the tannic extract, there was 69.45 % degradation, and a 100 % discoloration in the indigo carmine. When applying the Aspergillus niger fungus to the tannic extract and orange II dye, there was a 63,45 % and 32,78 % degradation, respectively. There was a discoloration no greater than 26 % when applying Trametes versicolor and Aspergillus niger to the red phenol. The fungi applied during the biological treatment presented tolerance to both the tannic extract composition as well as the concentration of each dye used, verified through growing kinetics of each fungus.Esta investigación se la realizó con el fin de evaluar la capacidad de biodegradación de los fenoles presentes en el extracto de tánico de guarango (Caesalpinia spinosa) y en los colorantes índigo carmín, naranja II y rojo fenol, presentes en los desechos de la industria textil. Se utilizaron dos tipos de hongos, el de podredumbre blanca Trametes versicolor y filamentoso Aspergillus niger. Los tratamientos biológicos se aplicaron por triplicado, realizados por el método espectrofotométrico UV por 15 días; los resultados obtenidos al aplicar el hongo Trametes versicolor en el extracto tánico fue del 69,45 % de degradación; y en el colorante índigo carmín fue del 100 % de decoloración. Los resultados de la degradación con el hongo Aspergillus niger sobre el extracto tánico fue del 63,45 % y en el colorante naranja II fue del 32,78 %. En la aplicación de Trametes versicolor y Aspergillus niger sobre el colorante rojo fenol se obtuvieron porcentajes de decoloración no mayores al 26 %. Los hongos aplicados en los tratamientos biológicos presentaron tolerancia tanto a la composición del extracto tánico como a las concentraciones de cada uno de los colorantes usados, esto fue comprobado con la cinética de crecimiento para cada hongo

Vojtěch Adam - One of the best experts on this subject based on the ideXlab platform.

  • Preparation and Optimisation of Cross-Linked Enzyme Aggregates Using Native Isolate White Rot Fungi Trametes versicolor and Fomes fomentarius for the Decolourisation of Synthetic Dyes
    International Journal of Environmental Research and Public Health, 2017
    Co-Authors: Martina Vršanská, Stanislava Voběrková, Ana Maria Jimenez Jimenez, Vladislav Strmiska, Vojtěch Adam
    Abstract:

    The key to obtaining an optimum performance of an enzyme is often a question of devising a suitable enzyme and optimisation of conditions for its immobilization. In this study, laccases from the native isolates of white rot fungi Fomes fomentarius and/or Trametes versicolor, obtained from Czech forests, were used. From these, cross-linked enzyme aggregates (CLEA) were prepared and characterised when the experimental conditions were optimized. Based on the optimization steps, saturated ammonium sulphate solution (75 wt.%) was used as the precipitating agent, and different concentrations of glutaraldehyde as a cross-linking agent were investigated. CLEA aggregates formed under the optimal conditions showed higher catalytic efficiency and stabilities (thermal, pH, and storage, against denaturation) as well as high reusability compared to free laccase for both fungal strains. The best concentration of glutaraldehyde seemed to be 50 mM and higher efficiency of cross-linking was observed at a low temperature 4 °C. An insignificant increase in optimum pH for CLEA laccases with respect to free laccases for both fungi was observed. The results show that the optimum temperature for both free laccase and CLEA laccase was 35 °C for T. versicolor and 30 °C for F. fomentarius. The CLEAs retained 80% of their initial activity for Trametes and 74% for Fomes after 70 days of cultivation. Prepared cross-linked enzyme aggregates were also investigated for their decolourisation activity on malachite green, bromothymol blue, and methyl red dyes. Immobilised CLEA laccase from Trametes versicolor showed 95% decolourisation potential and CLEA from Fomes fomentarius demonstrated 90% decolourisation efficiency within 10 h for all dyes used. These results suggest that these CLEAs have promising potential in dye decolourisation.

Yang Yang - One of the best experts on this subject based on the ideXlab platform.

  • enhancing the laccase production and laccase gene expression in the white rot fungus Trametes velutina 5930 with great potential for biotechnological applications by different metal ions and aromatic compounds
    PLOS ONE, 2013
    Co-Authors: Mulan Jiang, Yang Yang, Rui Zhuo, Chen Zhang, Xiaoyu Zhang
    Abstract:

    Laccase is useful for various biotechnological and industrial applications. The white-rot fungus Trametes velutina 5930 and its laccase, isolated from the Shennongjia Nature Reserve in China by our laboratory, has great potential for practical application in environmental biotechnology. However, the original level of laccase produced by Trametes velutina 5930 was relatively low in the absence of any inducer. Therefore, in order to enhance the laccase production by Trametes velutina 5930 and make better use of this fungus in the field of environmental biotechnology, the regulation of laccase production and laccase gene expression in Trametes velutina 5930 were investigated in this study. Different metal ions such as Cu2+ and Fe2+ could stimulate the laccase synthesis and laccase gene transcription in Trametes velutina 5930. Some aromatic compounds structurally related to lignin, such as tannic acid, syringic acid, cinnamic acid, gallic acid and guaiacol, could also enhance the level of laccase activity and laccase gene transcription. We also found that there existed a positive synergistic effect of aromatic compound and metal ion on the laccase production and laccase gene transcription in Trametes velutina 5930. Taken together, our study may contribute to the improvement of laccase productivity by Trametes velutina 5930.

  • characterization of a laccase gene from the white rot fungi Trametes sp 5930 isolated from shennongjia nature reserve in china and studying on the capability of decolorization of different synthetic dyes
    Biochemical Engineering Journal, 2011
    Co-Authors: Xiaoyu Zhang, Yang Yang, Fangfang Fan, Xia Wan, Mulan Jiang
    Abstract:

    Abstract Laccase belongs to a family of multi-copper oxidases which is especially useful for biotechnological and industrial applications. A laccase-producing white-rot fungi strain designated as Trametes sp. 5930 was nearly isolated from Shennongjia Nature Reserve in China. Trametes sp. 5930 had the high yield of laccase and was capable of decolorizing different dyes efficiently. Laccase played a very important role in the decolorization of different dyes by this fungus. The laccase gene lac5930-1 and its corresponding full-length cDNA were then cloned and characterized from Trametes sp. 5930. The 1563 bp full-length cDNA of lac5930-1 encoded a mature laccase protein consisting of 499 amino acids preceded by a signal peptide of 21 amino acids. lac5930-1 gene was successfully expressed in Pichia pastoris , which verified the function of lac5930-1 encoding active laccase by means of gene expression. The recombinant laccase produced by the yeast transformant in which lac5930-1 was efficiently expressed, conferred the ability to decolorize different dyes. The capability of decolorizing different dyes was positively related to the laccase activity, which provided strong evidence for the important function of laccase used in decolorizing industrial dyes.

Huachi Laura - One of the best experts on this subject based on the ideXlab platform.

  • BIODEGRADACIÓN DE LOS FENOLES PRESENTES EN EL EXTRACTO DE GUARANGO(Caesalpinia Spinosa) Y EN LOS COLIRANTES ÍNDIGO CARMÍN, NARANJA II Y ROJO FENOL A TRAVÉZ DE Trametes Versicolor AND Aspergillus Niger.
    2014
    Co-Authors: Huachi Laura, Macas, Ángela Macas, Méndez Gabriela
    Abstract:

    Esta investigación se la realizó con el fin de evaluar la capacidad de biodegradación de los fenoles presentes en el extracto de tánico de guarango((Caesalpina spinosa) y en los colorantes índigo carmín, naranja II y el rojo fenol, presentes en los desechos de la industria textil. Se utilizaron dos tipos de hongos, el de podredumbr blanca Trametes versicolor y filamentos Aspergillus niger : Los tratamientos bológicos se aplicaron por triplicado, reaoizados por el método expectrofotométrico Uv por 15 días; los resultados obtenidos al aplicar el hongo Trametes versicolor en el extracto titáico fue del 69.45% de degradación; y en el colorante índigo carmín fue del 100% de decoloración. Los resultados de la degradación de Trametes versicolor y Aspergillus niger sobre el colorante rojo fenol se obtuvieron porcentajes de decoloración no mayores al 26%. Los hongos aplicados en los tratamientos biológicos presentaron tolerancia tanto a la composición del extracto tánico como a las concentraciones de cada uno de los colorantes usados, esto fue comprobado con la cinética de crecimiento para cada hongo.The research was done to evaluate phenol biodregadation capacity in Guarango(Caesalpina spinosa) tannic extract phenols and in indigo carmine, orange II and red phenols, common residues in the textile industry. Two types of fungi were used, white-rot Trametes versicolor fungus and the filamentous Aspergillus niger. Biological treatments were applied in triplicate trough the spectrophotometric UV method for 15 days. When applying the Trametes versicolor fungis to the tannic extract, there was 69.45% degradation, and a 100% discoloration in the indigo carmine. When applying Aspergillus niger fungus to the tannic extract and orange II dye, there was a 63.45% and 32.78% degradatión, respectively. There was a discoloration no greater than 26% when applying Trametes versicolor and Aspergillus niger to the red phenol. The fungi applied during the biological treatement presented tolerance to both the tannic extract composition as well as the concentration of each dye used, verified through growing kinetics of each fungu

  • GUARANGO EXTRACT (Caesalpinia Spinosa) AND INDIGO CARMINE, ORANGE II, AND RED DYES PHENOL BIODEGRADATION THROUGH Trametes Versicolor AND Aspergillus Niger
    'Salesian Polytechnic University of Ecuador', 2014
    Co-Authors: Huachi Laura, Macas Ángela, Méndez Gabriela
    Abstract:

    Esta investigación se la realizó con el fin de evaluar la capacidad de biodegradación de los fenoles presentes en el extracto de tánico de guarango (Caesalpinia spinosa) y en los colorantes índigo carmín, naranja II y rojo fenol, presentes en los desechos de la industria textil. Se utilizaron dos tipos de hongos, el de podredumbre blanca Trametes versicolor y filamentoso Aspergillus niger. Los tratamientos biológicos se aplicaron por triplicado, realizados por el método espectrofotométrico UV por 15 días; los resultados obtenidos al aplicar el hongo Trametes versicolor en el extracto tánico fue del 69,45 % de degradación; y en el colorante índigo carmín fue del 100 % de decoloración. Los resultados de la degradación con el hongo Aspergillus niger sobre el extracto tánico fue del 63,45 % y en el colorante naranja II fue del 32,78 %. En la aplicación de Trametes versicolor y Aspergillus niger sobre el colorante rojo fenol se obtuvieron porcentajes de decoloración no mayores al 26 %. Los hongos aplicados en los tratamientos biológicos presentaron tolerancia tanto a la composición del extracto tánico como a las concentraciones de cada uno de los colorantes usados, esto fue comprobado con la cinética de crecimiento para cada hongo.This research was done to evaluate phenol biodegradation capacity in Guarango (Caesalpinia spinosa) tannic extract phenols and in indigo carmine, orange II and red phenols, common residues in the textile industry. Two types of fungi were used, white-rot Trametes versicolor fungus and the filamentous Aspergillus niger. Biological treatments were applied in triplicate through the spectrophotometric UV method for 15 days. When applying the Trametes versicolor fungus to the tannic extract, there was 69.45 % degradation, and a 100 % discoloration in the indigo carmine. When applying the Aspergillus niger fungus to the tannic extract and orange II dye, there was a 63,45 % and 32,78 % degradation, respectively. There was a discoloration no greater than 26 % when applying Trametes versicolor and Aspergillus niger to the red phenol. The fungi applied during the biological treatment presented tolerance to both the tannic extract composition as well as the concentration of each dye used, verified through growing kinetics of each fungus

  • Biodegradación de los fenoles presentes en el extracto de guarango (caesalpinia spinosa) y en los colorantes índigo carmín, naranja ii y rojo fenol a través de Trametes versicolor y aspergillus niger
    2014
    Co-Authors: Huachi Laura, Macías Ángel, Méndez Gabriela
    Abstract:

    This research was done to evaluate phenol biodegradation capacity in Guarango (Caesalpinia spinosa) tannic extract phenols and in indigo carmine, orange II and red phenols, common residues in the textile industry. Two types of fungi were used, white-rot Trametes versicolor fungus and the filamentous Aspergillus niger. Biological treatments were applied in triplicate through the spectrophotometric UV method for 15 days. When applying the Trametes versicolor fungus to the tannic extract, there was 69.45 % degradation, and a 100 % discoloration in the indigo carmine. When applying the Aspergillus niger fungus to the tannic extract and orange II dye, there was a 63,45 % and 32,78 % degradation, respectively. There was a discoloration no greater than 26 % when applying Trametes versicolor and Aspergillus niger to the red phenol. The fungi applied during the biological treatment presented tolerance to both the tannic extract composition as well as the concentration of each dye used, verified through growing kinetics of each fungus.Esta investigación se la realizó con el fin de evaluar la capacidad de biodegradación de los fenoles presentes en el extracto de tánico de guarango (Caesalpinia spinosa) y en los colorantes índigo carmín, naranja II y rojo fenol, presentes en los desechos de la industria textil. Se utilizaron dos tipos de hongos, el de podredumbre blanca Trametes versicolor y filamentoso Aspergillus niger. Los tratamientos biológicos se aplicaron por triplicado, realizados por el método espectrofotométrico UV por 15 días; los resultados obtenidos al aplicar el hongo Trametes versicolor en el extracto tánico fue del 69,45 % de degradación; y en el colorante índigo carmín fue del 100 % de decoloración. Los resultados de la degradación con el hongo Aspergillus niger sobre el extracto tánico fue del 63,45 % y en el colorante naranja II fue del 32,78 %. En la aplicación de Trametes versicolor y Aspergillus niger sobre el colorante rojo fenol se obtuvieron porcentajes de decoloración no mayores al 26 %. Los hongos aplicados en los tratamientos biológicos presentaron tolerancia tanto a la composición del extracto tánico como a las concentraciones de cada uno de los colorantes usados, esto fue comprobado con la cinética de crecimiento para cada hongo

Mulan Jiang - One of the best experts on this subject based on the ideXlab platform.

  • characterization of the factors that influence sinapine concentration in rapeseed meal during fermentation
    PLOS ONE, 2015
    Co-Authors: Yanxing Niu, Mulan Jiang, Mian Guo, Chuyun Wan, Hu Jin, Fenghong Huang
    Abstract:

    We analyzed and compared the difference in sinapine concentration in rapeseed meal between the filamentous fungus, Trametes sp 48424, and the yeast, Saccharomyces cerevisiae, in both liquid and solid-state fermentation. During liquid and solid-state fermentation by Trametes sp 48424, the sinapine concentration decreased significantly. In contrast, the liquid and solid-state fermentation process by Saccharomyces cerevisiae just slightly decreased the sinapine concentration (P ≤ 0.05). After the solid-state fermented samples were dried, the concentration of sinapine in rapeseed meal decreased significantly in Saccharomyces cerevisiae. Based on the measurement of laccase activity, we observed that laccase induced the decrease in the concentration of sinapine during fermentation with Trametes sp 48424. In order to eliminate the influence of microorganisms and the metabolites produced during fermentation, high moisture rapeseed meal and the original rapeseed meal were dried at 90°C and 105°C, respectively. During drying, the concentration of sinapine in high moisture rapeseed meal decreased rapidly and we obtained a high correlation coefficient between the concentration of sinapine and loss of moisture. Our results suggest that drying and enzymes, especially laccase that is produced during the solid-state fermentation process, may be the main factors that affect the concentration of sinapine in rapeseed meal.

  • enhancing the laccase production and laccase gene expression in the white rot fungus Trametes velutina 5930 with great potential for biotechnological applications by different metal ions and aromatic compounds
    PLOS ONE, 2013
    Co-Authors: Mulan Jiang, Yang Yang, Rui Zhuo, Chen Zhang, Xiaoyu Zhang
    Abstract:

    Laccase is useful for various biotechnological and industrial applications. The white-rot fungus Trametes velutina 5930 and its laccase, isolated from the Shennongjia Nature Reserve in China by our laboratory, has great potential for practical application in environmental biotechnology. However, the original level of laccase produced by Trametes velutina 5930 was relatively low in the absence of any inducer. Therefore, in order to enhance the laccase production by Trametes velutina 5930 and make better use of this fungus in the field of environmental biotechnology, the regulation of laccase production and laccase gene expression in Trametes velutina 5930 were investigated in this study. Different metal ions such as Cu2+ and Fe2+ could stimulate the laccase synthesis and laccase gene transcription in Trametes velutina 5930. Some aromatic compounds structurally related to lignin, such as tannic acid, syringic acid, cinnamic acid, gallic acid and guaiacol, could also enhance the level of laccase activity and laccase gene transcription. We also found that there existed a positive synergistic effect of aromatic compound and metal ion on the laccase production and laccase gene transcription in Trametes velutina 5930. Taken together, our study may contribute to the improvement of laccase productivity by Trametes velutina 5930.

  • characterization of a laccase gene from the white rot fungi Trametes sp 5930 isolated from shennongjia nature reserve in china and studying on the capability of decolorization of different synthetic dyes
    Biochemical Engineering Journal, 2011
    Co-Authors: Xiaoyu Zhang, Yang Yang, Fangfang Fan, Xia Wan, Mulan Jiang
    Abstract:

    Abstract Laccase belongs to a family of multi-copper oxidases which is especially useful for biotechnological and industrial applications. A laccase-producing white-rot fungi strain designated as Trametes sp. 5930 was nearly isolated from Shennongjia Nature Reserve in China. Trametes sp. 5930 had the high yield of laccase and was capable of decolorizing different dyes efficiently. Laccase played a very important role in the decolorization of different dyes by this fungus. The laccase gene lac5930-1 and its corresponding full-length cDNA were then cloned and characterized from Trametes sp. 5930. The 1563 bp full-length cDNA of lac5930-1 encoded a mature laccase protein consisting of 499 amino acids preceded by a signal peptide of 21 amino acids. lac5930-1 gene was successfully expressed in Pichia pastoris , which verified the function of lac5930-1 encoding active laccase by means of gene expression. The recombinant laccase produced by the yeast transformant in which lac5930-1 was efficiently expressed, conferred the ability to decolorize different dyes. The capability of decolorizing different dyes was positively related to the laccase activity, which provided strong evidence for the important function of laccase used in decolorizing industrial dyes.