The Experts below are selected from a list of 3594 Experts worldwide ranked by ideXlab platform
Sun-mi Lee - One of the best experts on this subject based on the ideXlab platform.
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Improved simultaneous co-fermentation of glucose and xylose by Saccharomyces cerevisiae for efficient Lignocellulosic Biorefinery.
Biotechnology for biofuels, 2020Co-Authors: Phuong Hoang Nguyen Tran, Gyeongtaek Gong, Sun-mi LeeAbstract:Lignocellulosic Biorefinery offers economical and sustainable production of fuels and chemicals. Saccharomyces cerevisiae, a promising industrial host for Biorefinery, has been intensively developed to expand its product profile. However, the sequential and slow conversion of xylose into target products remains one of the main challenges for realizing efficient industrial Lignocellulosic Biorefinery. In this study, we developed a powerful mixed-sugar co-fermenting strain of S. cerevisiae, XUSEA, with improved xylose conversion capacity during simultaneous glucose/xylose co-fermentation. To reinforce xylose catabolism, the overexpression target in the pentose phosphate pathway was selected using a DNA assembler method and overexpressed increasing xylose consumption and ethanol production by twofold. The performance of the newly engineered strain with improved xylose catabolism was further boosted by elevating fermentation temperature and thus significantly reduced the co-fermentation time by half. Through combined efforts of reinforcing the pathway of xylose catabolism and elevating the fermentation temperature, XUSEA achieved simultaneous co-fermentation of Lignocellulosic hydrolysates, composed of 39.6 g L-1 glucose and 23.1 g L-1 xylose, within 24 h producing 30.1 g L-1 ethanol with a yield of 0.48 g g-1. Owing to its superior co-fermentation performance and ability for further engineering, XUSEA has potential as a platform in a Lignocellulosic Biorefinery toward realizing a more economical and sustainable process for large-scale bioethanol production. © The Author(s) 2020.
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improved simultaneous co fermentation of glucose and xylose by saccharomyces cerevisiae for efficient Lignocellulosic Biorefinery
Biotechnology for Biofuels, 2020Co-Authors: Phuong Hoang Nguyen Tran, Gyeongtaek Gong, Sun-mi LeeAbstract:Lignocellulosic Biorefinery offers economical and sustainable production of fuels and chemicals. Saccharomyces cerevisiae, a promising industrial host for Biorefinery, has been intensively developed to expand its product profile. However, the sequential and slow conversion of xylose into target products remains one of the main challenges for realizing efficient industrial Lignocellulosic Biorefinery. In this study, we developed a powerful mixed-sugar co-fermenting strain of S. cerevisiae, XUSEA, with improved xylose conversion capacity during simultaneous glucose/xylose co-fermentation. To reinforce xylose catabolism, the overexpression target in the pentose phosphate pathway was selected using a DNA assembler method and overexpressed increasing xylose consumption and ethanol production by twofold. The performance of the newly engineered strain with improved xylose catabolism was further boosted by elevating fermentation temperature and thus significantly reduced the co-fermentation time by half. Through combined efforts of reinforcing the pathway of xylose catabolism and elevating the fermentation temperature, XUSEA achieved simultaneous co-fermentation of Lignocellulosic hydrolysates, composed of 39.6 g L−1 glucose and 23.1 g L−1 xylose, within 24 h producing 30.1 g L−1 ethanol with a yield of 0.48 g g−1. Owing to its superior co-fermentation performance and ability for further engineering, XUSEA has potential as a platform in a Lignocellulosic Biorefinery toward realizing a more economical and sustainable process for large-scale bioethanol production.
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Complete Genome Sequence of Paenibacillus sp. CAA11: A Promising Microbial Host for Lignocellulosic Biorefinery with Consolidated Processing.
Current microbiology, 2019Co-Authors: Gyeongtaek Gong, Sukhyeong Cho, Seil Kim, Sun-mi LeeAbstract:Several bioprocessing technologies, such as separate hydrolysis and fermentation (SHF), simultaneous saccharification and fermentation (SSF), and consolidated bioprocessing (CBP), have been highlighted to produce bio-based fuels and chemicals from Lignocellulosic biomass. Successful CBP, an efficient and economical Lignocellulosic Biorefinery process compared with other processes, requires microorganisms with sufficient cellulolytic activity and biofuel/chemical-producing ability. Here, we report the complete genome of Paenibacillus sp. CAA11, a newly isolated promising microbial host for CBP-producing ethanol and organic acids from cellulose. The genome of Paenibacillus sp. CAA11 comprises one 4,888,410 bp chromosome with a G + C content of 48.68% containing 4418 protein-coding genes, 102 tRNA genes, and 39 rRNA genes. The functionally active cellulase, encoded by CAA_GH5 was identified to belong to glycosyl hydrolase family 5 (GH5) and consisted of a catalytic domain and a cellulose-binding domain 3 (CBM3). When cellulolytic activity of CAA_GH5 was assayed through Congo red method by measuring the size of halo zone, the recombinant Bacillus subtilis RIK1285 expressing CAA_GH5 showed a comparable cellulolytic activity to B. subtilis RIK1285 expressing Cel5, a previously verified powerful bacterial cellulase. This study demonstrates the potential of Paenibacillus sp. CAA11 as a CBP-enabling microbe for cost-effective biofuels/chemicals production from Lignocellulosic biomass.
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Genomic and phenotypic characterization of a refactored xylose-utilizing Saccharomyces cerevisiae strain for Lignocellulosic biofuel production.
Biotechnology for biofuels, 2018Co-Authors: Phuong Tran Nguyen Hoang, Gyeongtaek Gong, Sun-mi LeeAbstract:Engineered strains of Saccharomyces cerevisiae have significantly improved the prospects of Biorefinery by improving the bioconversion yields in Lignocellulosic bioethanol production and expanding the product profiles to include advanced biofuels and chemicals. However, the Lignocellulosic Biorefinery concept has not been fully applied using engineered strains in which either xylose utilization or advanced biofuel/chemical production pathways have been upgraded separately. Specifically, high-performance xylose-fermenting strains have rarely been employed as advanced biofuel and chemical production platforms and require further engineering to expand their product profiles. In this study, we refactored a high-performance xylose-fermenting S. cerevisiae that could potentially serve as a platform strain for advanced biofuels and biochemical production. Through combinatorial CRISPR–Cas9-mediated rational and evolutionary engineering, we obtained a newly refactored isomerase-based xylose-fermenting strain, XUSE, that demonstrated efficient conversion of xylose into ethanol with a high yield of 0.43 g/g. In addition, XUSE exhibited the simultaneous fermentation of glucose and xylose with negligible glucose inhibition, indicating the potential of this isomerase-based xylose-utilizing strain for Lignocellulosic Biorefinery. The genomic and transcriptomic analysis of XUSE revealed beneficial mutations and changes in gene expression that are responsible for the enhanced xylose fermentation performance of XUSE. In this study, we developed a high-performance xylose-fermenting S. cerevisiae strain, XUSE, with high ethanol yield and negligible glucose inhibition. Understanding the genomic and transcriptomic characteristics of XUSE revealed isomerase-based engineering strategies for improved xylose fermentation in S. cerevisiae. With high xylose fermentation performance and room for further engineering, XUSE could serve as a promising platform strain for Lignocellulosic Biorefinery.
Johan P M Sanders - One of the best experts on this subject based on the ideXlab platform.
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lignin depolymerisation in supercritical carbon dioxide acetone water fluid for the production of aromatic chemicals
Bioresource Technology, 2012Co-Authors: Richard J A Gosselink, Wouter Teunissen, Ed De Jong, Elinor L Scott, Göran Gellerstedt, Johan P M SandersAbstract:Valorisation of lignin plays a key role in further development of Lignocellulosic Biorefinery processes the production of biofuels and bio-based materials. In the present study, organosolv hardwood and wheat straw lignins were converted in a supercritical fluid consisting of carbon dioxide/acetone/water (300–370 °C, 100 bar) to a phenolic oil consisting of oligomeric fragments and monomeric aromatic compounds with a total yield of 10–12% based on lignin. These yields are similar to the state-of-the-art technologies such as base-catalysed thermal processes applied for lignin depolymerisation. Addition of formic acid increases the yield of monomeric aromatic species by stabilizing aromatic radicals. Supercritical depolymerisation of wheat straw and hardwood lignin yielded monomeric compounds in different compositions with a maximum yield of 2.0% for syringic acid and 3.6% for syringol, respectively. The results of the present study showed that under the applied conditions competition occurred between lignin depolymerisation and recondensation of fragments.
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Lignin depolymerisation in supercritical carbon dioxide/acetone/water fluid for the production of aromatic chemicals.
Bioresource Technology, 2011Co-Authors: Richard J A Gosselink, Wouter Teunissen, Ed De Jong, Elinor L Scott, Göran Gellerstedt, Johan P M SandersAbstract:Valorisation of lignin plays a key role in further development of Lignocellulosic Biorefinery processes the production of biofuels and bio-based materials. In the present study, organosolv hardwood and wheat straw lignins were converted in a supercritical fluid consisting of carbon dioxide/acetone/water (300–370 °C, 100 bar) to a phenolic oil consisting of oligomeric fragments and monomeric aromatic compounds with a total yield of 10–12% based on lignin. These yields are similar to the state-of-the-art technologies such as base-catalysed thermal processes applied for lignin depolymerisation. Addition of formic acid increases the yield of monomeric aromatic species by stabilizing aromatic radicals. Supercritical depolymerisation of wheat straw and hardwood lignin yielded monomeric compounds in different compositions with a maximum yield of 2.0% for syringic acid and 3.6% for syringol, respectively. The results of the present study showed that under the applied conditions competition occurred between lignin depolymerisation and recondensation of fragments.
Richard J A Gosselink - One of the best experts on this subject based on the ideXlab platform.
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lignin depolymerisation in supercritical carbon dioxide acetone water fluid for the production of aromatic chemicals
Bioresource Technology, 2012Co-Authors: Richard J A Gosselink, Wouter Teunissen, Ed De Jong, Elinor L Scott, Göran Gellerstedt, Johan P M SandersAbstract:Valorisation of lignin plays a key role in further development of Lignocellulosic Biorefinery processes the production of biofuels and bio-based materials. In the present study, organosolv hardwood and wheat straw lignins were converted in a supercritical fluid consisting of carbon dioxide/acetone/water (300–370 °C, 100 bar) to a phenolic oil consisting of oligomeric fragments and monomeric aromatic compounds with a total yield of 10–12% based on lignin. These yields are similar to the state-of-the-art technologies such as base-catalysed thermal processes applied for lignin depolymerisation. Addition of formic acid increases the yield of monomeric aromatic species by stabilizing aromatic radicals. Supercritical depolymerisation of wheat straw and hardwood lignin yielded monomeric compounds in different compositions with a maximum yield of 2.0% for syringic acid and 3.6% for syringol, respectively. The results of the present study showed that under the applied conditions competition occurred between lignin depolymerisation and recondensation of fragments.
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Lignin depolymerisation in supercritical carbon dioxide/acetone/water fluid for the production of aromatic chemicals.
Bioresource Technology, 2011Co-Authors: Richard J A Gosselink, Wouter Teunissen, Ed De Jong, Elinor L Scott, Göran Gellerstedt, Johan P M SandersAbstract:Valorisation of lignin plays a key role in further development of Lignocellulosic Biorefinery processes the production of biofuels and bio-based materials. In the present study, organosolv hardwood and wheat straw lignins were converted in a supercritical fluid consisting of carbon dioxide/acetone/water (300–370 °C, 100 bar) to a phenolic oil consisting of oligomeric fragments and monomeric aromatic compounds with a total yield of 10–12% based on lignin. These yields are similar to the state-of-the-art technologies such as base-catalysed thermal processes applied for lignin depolymerisation. Addition of formic acid increases the yield of monomeric aromatic species by stabilizing aromatic radicals. Supercritical depolymerisation of wheat straw and hardwood lignin yielded monomeric compounds in different compositions with a maximum yield of 2.0% for syringic acid and 3.6% for syringol, respectively. The results of the present study showed that under the applied conditions competition occurred between lignin depolymerisation and recondensation of fragments.
Wolter Prins - One of the best experts on this subject based on the ideXlab platform.
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Production and characterization of slow pyrolysis biochar from lignin-rich digested stillage from Lignocellulosic ethanol production
Biomass and Bioenergy, 2019Co-Authors: Stef Ghysels, Frederik Ronsse, Dane Dickinson, Wolter PrinsAbstract:Abstract Lignin-rich digested stillage as a novel feedstock for biochar production was subjected to slow pyrolysis. The lignin residue stemmed from a Lignocellulosic ethanol pilot run with poplar, from which the stillage was anaerobically digested prior to pyrolysis. Pyrolysis conditions were optimized to meet guidelines put forth by the International Biochar Initiative and the European Biochar Certificate (molar H/Corg ratio 0.7 and O/Corg ratio 0.4 ), as biochar could be carbon-negative upon soil amendment, while reallocating nutrients to the field. Bench-scale pyrolysis of the lignin residue and straw were conducted according to a 33 factorial design with center runs (ranges: 370–450 °C highest treatment temperature, 5–45 min holding time and 5–50 °C.min−1 heating rate). Parametric and nonparametric statistics revealed that the highest treatment temperature was by far the most influencing factor for both feedstocks which ‘pushed’ H/C and O/C ratios within the desired range. Lignin-based biochar can be obtained with 50.7% yield, a H/C ratio of 0.70 and an O/C ratio of 0.20, already at 384 °C. This is considerably better when compared to straw-based biochar with identical H/C and O/C ratios, which came with a lower yield (33.7%) and required a modestly higher temperature (410 °C). Results from this study emphasize the feasibility to integrate slow pyrolysis in a Lignocellulosic Biorefinery.
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production and characterization of slow pyrolysis biochar from lignin rich digested stillage from Lignocellulosic ethanol production
ISSN: 0961-9534, 2019Co-Authors: Stef Ghysels, Frederik Ronsse, Dane Dickinson, Wolter PrinsAbstract:Lignin-rich digested stillage as a novel feedstock for biochar production was subjected to slow pyrolysis. The lignin residue stemmed from a Lignocellulosic ethanol pilot run with poplar, from which the stillage was anaerobically digested prior to pyrolysis. Pyrolysis conditions were optimized to meet guidelines put forth by the International Biochar Initiative and the European Biochar Certificate (molar H/C-org ratio and O/C-org ratio <0.4), as biochar could be carbon-negative upon soil amendment, while reallocating nutrients to the field. Bench-scale pyrolysis of the lignin residue and straw were conducted according to a 3(3) factorial design with center runs (ranges: 370-450 degrees C highest treatment temperature, 5-45 min holding time and 5-50 degrees C.min(-1) heating rate). Parametric and nonparametric statistics revealed that the highest treatment temperature was by far the most influencing factor for both feedstocks which 'pushed' H/C and O/C ratios within the desired range. Lignin-based biochar can be obtained with 50.7% yield, a H/C ratio of 0.70 and an O/C ratio of 0.20, already at 384 degrees C. This is considerably better when compared to straw-based biochar with identical H/C and O/C ratios, which came with a lower yield (33.7%) and required a modestly higher temperature (410 degrees C). Results from this study emphasize the feasibility to integrate slow pyrolysis in a Lignocellulosic Biorefinery.
Elinor L Scott - One of the best experts on this subject based on the ideXlab platform.
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lignin depolymerisation in supercritical carbon dioxide acetone water fluid for the production of aromatic chemicals
Bioresource Technology, 2012Co-Authors: Richard J A Gosselink, Wouter Teunissen, Ed De Jong, Elinor L Scott, Göran Gellerstedt, Johan P M SandersAbstract:Valorisation of lignin plays a key role in further development of Lignocellulosic Biorefinery processes the production of biofuels and bio-based materials. In the present study, organosolv hardwood and wheat straw lignins were converted in a supercritical fluid consisting of carbon dioxide/acetone/water (300–370 °C, 100 bar) to a phenolic oil consisting of oligomeric fragments and monomeric aromatic compounds with a total yield of 10–12% based on lignin. These yields are similar to the state-of-the-art technologies such as base-catalysed thermal processes applied for lignin depolymerisation. Addition of formic acid increases the yield of monomeric aromatic species by stabilizing aromatic radicals. Supercritical depolymerisation of wheat straw and hardwood lignin yielded monomeric compounds in different compositions with a maximum yield of 2.0% for syringic acid and 3.6% for syringol, respectively. The results of the present study showed that under the applied conditions competition occurred between lignin depolymerisation and recondensation of fragments.
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Lignin depolymerisation in supercritical carbon dioxide/acetone/water fluid for the production of aromatic chemicals.
Bioresource Technology, 2011Co-Authors: Richard J A Gosselink, Wouter Teunissen, Ed De Jong, Elinor L Scott, Göran Gellerstedt, Johan P M SandersAbstract:Valorisation of lignin plays a key role in further development of Lignocellulosic Biorefinery processes the production of biofuels and bio-based materials. In the present study, organosolv hardwood and wheat straw lignins were converted in a supercritical fluid consisting of carbon dioxide/acetone/water (300–370 °C, 100 bar) to a phenolic oil consisting of oligomeric fragments and monomeric aromatic compounds with a total yield of 10–12% based on lignin. These yields are similar to the state-of-the-art technologies such as base-catalysed thermal processes applied for lignin depolymerisation. Addition of formic acid increases the yield of monomeric aromatic species by stabilizing aromatic radicals. Supercritical depolymerisation of wheat straw and hardwood lignin yielded monomeric compounds in different compositions with a maximum yield of 2.0% for syringic acid and 3.6% for syringol, respectively. The results of the present study showed that under the applied conditions competition occurred between lignin depolymerisation and recondensation of fragments.