The Experts below are selected from a list of 2133 Experts worldwide ranked by ideXlab platform
Stephen E Feinberg - One of the best experts on this subject based on the ideXlab platform.
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In Vitro Development of a Mucocutaneous Junction for Lip Reconstruction.
Journal of Oral and Maxillofacial Surgery, 2016Co-Authors: Gurkan Rasit Bayar, Cassou Lozada Y Marcelo, Stephen E FeinbergAbstract:Purpose We present a straightforward and reproducible technique to create, in vitro, a construct containing a mucocutaneous junction (MCJ) with a transitional zone (vermilion) for fabrication of a microvascular prelaminated flap for use in Lip Reconstruction. Materials and Methods Cultured primary human skin keratinocytes and oral mucosal epithelial cells at premixed ratios of 50% skin cells to 50% oral cells, 25% skin cells to 75% oral cells, and 75% skin cells to 25% oral cells were grown on an AlloDerm dermal equivalent (LifeCell, Branchburg, NJ) to create an MCJ equivalent with a Lip or transitional zone (vermilion) using a novel 3-dimensional (3D) culture device with a barrier to separate co-cultured skin and oral cells. The 3 different cell ratios were compared by staining for the following specific differentiation markers to define the different areas of skin and mucosal keratinocytes: filaggrin, cytokeratin 10, cytokeratin 19, and small proline-rich protein 3. Results Immunohistochemical results showed that MCJ equivalents seeded with premixed cells were similar to the differentiation patterns of tissue-engineered 3D cultures using 100% oral mucosal epithelial cells or skin keratinocytes. The engineered MCJ-equivalent constructs, grown in the 3D device specifically constructed with a cell-free gap at the barrier site, formed a transitional zone (vermilion) at the barrier site with intermingling of the skin and oral keratinocytes. The results showed different and unique expression patterns of filaggrin, cytokeratin 10, cytokeratin 19, and small proline-rich protein 3 by those cells migrating into the gap, which were similar to those seen in human Lip tissue. This pattern was not seen in MCJ equivalents created using premixed skin and oral cells. Conclusions Using a device to separately co-culture human oral and skin keratinocytes to allow the cells to migrate into a cell-free zone resulted in phenotypic expression closer to what is seen in native tissue, in comparison to premixing the skin and oral cells before seeding.
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tissue engineering of Lips and muco cutaneous junctions in vitro development of tissue engineered constructs of oral mucosa and skin for Lip Reconstruction
Tissue Engineering Part C-methods, 2012Co-Authors: Antonio Peramo, Cynthia L Marcelo, Stephen E FeinbergAbstract:We report for the first time the fabrication of a three-dimensional tissue structure containing, in a continuous layer, the morphological features of a Lip: epidermal skin, vermillion, and oral mucosa. This tissue engineered muco-cutaneous (M/C) equivalent was manufactured using human oral and skin keratinocytes grown on an acellular, nonimmunogenic dermal equivalent (AlloDerm®) to produce a tissue equivalent with similar anatomic and handling properties as native human Lips. Confirmation of the structural composition of the construct was performed using routine histology and immunohistochemistry by identification of epithelial markers that are differentially expressed in separate anatomic areas of the Lips. These full-thickness human Lip skin equivalents can be used in surgical Lip Reconstruction in individuals suffering from Lip loss from cancer, congenital deformations, and injuries after accidents. We propose this technique can be used as a general basis for tissue engineering of M/C junctions in othe...
Elke Van De Casteele - One of the best experts on this subject based on the ideXlab platform.
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Secondary Cleft Lip Reconstruction and the Use of Pedicled, Deepithelialized Scar Tissue.
Plastic and reconstructive surgery. Global open, 2016Co-Authors: Nasser Nadjmi, Sara Amadori, Elke Van De CasteeleAbstract:Background The optimal time to create symmetry in a cleft Lip is during primary repair; a secondary effort later is more difficult due to potential scarring and possible tissue deficiency of the repaired cleft Lip. A plethora of methods for secondary correction have been described that have the goal of constructing the philtral column, ameliorating bad scar results, and augmenting Lip volume, for example. Nevertheless, there is no single procedure that yields completely satisfactory results. In addition, the appropriate timing for secondary surgical corrections of the cleft Lip is still under debate. Methods We present a new technique for secondary Lip Reconstruction of unilateral and bilateral cleft patients using pedicled, de-epithelialized cleft scar tissue as an autologous graft to obtain sustainable Lip volume. Our results were evaluated by physicians and patient-parent satisfaction surveys. Results The esthetic outcomes of 29 patients were assessed using a patient satisfaction questionnaire and a physician survey based on the preoperative and postoperative clinical images. The success of the procedure was evaluated using a 5-point scale. The total scores of both the physician and patient assessments were high, although no correlation was found between the scores. Conclusions Cleft Lip Reconstruction using pedicled, deepithelialized scar tissue leads to excellent physician and patient satisfaction scores; this technique can be executed at any patient age and as a secondary repair for any given primary type of cleft disorder.
Yu Jian - One of the best experts on this subject based on the ideXlab platform.
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Strategy for Correction of the Whistling Deformity in Secondary Cleft Lip Reconstruction.
Plastic and reconstructive surgery. Global open, 2020Co-Authors: Xinchun Jian, Yu JianAbstract:Background Following primary repair of a cleft Lip, patients present with many facial deformities. One of the commonly observed sequelae of cleft Lip repair is a whistling deformity. This retrospective study was carried out to evaluate the outcomes following correction of whistling deformities in secondary cleft Lip Reconstruction. Methods We retrospectively reviewed the hospital records of patients with various whistling deformities who underwent repair from April 1989 to March 2018; 2 surgeons performed the repair using either the double movable mucomuscular complex flaps technique, modified Abbe flap technique, or Abbe flap technique. The postoperative anatomical structure and aesthetic effects of the surgery were evaluated. Results In total, 136 patients were included in this study. Among these patients, 60 (44.2%) had a grade I whistling deformity and 47 (34.5%) had a grade II deformity and repair was performed using the double movable mucomuscular complex flaps technique and modified Abbe flap transfer technique, respectively, whereas the Abbe flap transfer technique was used in 16 patients (11.8%) and 13 patients (9.5%) with a grade III and grade IV whistling deformity, respectively. All patients were found to have normal postoperative anatomical structures and aesthetic effects of the upper Lip, with all patients experiencing mild to moderate postoperative edema of the upper Lip, and 29 cases (21.3%) developed an inconspicuous scar. Conclusion The repair technique should be chosen based on the type of whistling deformity.
Wilhelm Schulte-mattler - One of the best experts on this subject based on the ideXlab platform.
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Stein's Double Cross-Lip Flaps Combined with Johanson's Step Technique for Subtotal Lower Lip Reconstruction.
Plastic and reconstructive surgery. Global open, 2016Co-Authors: J. Camilo Roldán, Wilhelm Schulte-mattlerAbstract:UNLABELLED In a previous study, a single cross-Lip flap (Abbe flap) combined with Johanson's step technique for repair of defects of more than 2/3 of the lower Lip was superior, in terms of aesthetic and functional outcome, compared with Bernard Webster-related techniques (cheek advancement). Herewith, a double cross-Lip flap (Stein procedure) is proposed for repair of subtotal lower Lip defects. A systematic review of the Stein procedure is provided. METHODS Two patients underwent a paramedian double cross-Lip flap, preserving the aesthetic subunit philtrum column combined with the Johanson's step technique. The aesthetic and functional outcomes and the surgical steps are demonstrated in the videos. An electromyographic study was performed 6 months and 4 years after surgery. A PubMed and a Google Scholar search were performed for the Stein procedure published in 1848. RESULTS Lip competence was achieved directly after sectioning of the cross-Lip pedicles in both patients. Lips progressivity expanded in the first 6 months. No microstomia was observed. Electromyography showed successful reinnervation of the transplanted muscles at 6 months. Four years after surgery, the electromyographic findings were consolidated. Since 1975, 7 articles on the double cross-Lip procedure have been published: 4 in English, 1 in French, and 2 in Japanese. None of those articles reported on any supplemental lower Lip advancement or on any electromyographic study. CONCLUSIONS The rationale of using 2 cross-Lip flaps and a Lip-cheek advancement according to Johanson seems to achieve functionally and aesthetically superior results compared with other techniques described for subtotal lower Lip Reconstruction.
Nasser Nadjmi - One of the best experts on this subject based on the ideXlab platform.
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Secondary Cleft Lip Reconstruction and the Use of Pedicled, Deepithelialized Scar Tissue.
Plastic and reconstructive surgery. Global open, 2016Co-Authors: Nasser Nadjmi, Sara Amadori, Elke Van De CasteeleAbstract:Background The optimal time to create symmetry in a cleft Lip is during primary repair; a secondary effort later is more difficult due to potential scarring and possible tissue deficiency of the repaired cleft Lip. A plethora of methods for secondary correction have been described that have the goal of constructing the philtral column, ameliorating bad scar results, and augmenting Lip volume, for example. Nevertheless, there is no single procedure that yields completely satisfactory results. In addition, the appropriate timing for secondary surgical corrections of the cleft Lip is still under debate. Methods We present a new technique for secondary Lip Reconstruction of unilateral and bilateral cleft patients using pedicled, de-epithelialized cleft scar tissue as an autologous graft to obtain sustainable Lip volume. Our results were evaluated by physicians and patient-parent satisfaction surveys. Results The esthetic outcomes of 29 patients were assessed using a patient satisfaction questionnaire and a physician survey based on the preoperative and postoperative clinical images. The success of the procedure was evaluated using a 5-point scale. The total scores of both the physician and patient assessments were high, although no correlation was found between the scores. Conclusions Cleft Lip Reconstruction using pedicled, deepithelialized scar tissue leads to excellent physician and patient satisfaction scores; this technique can be executed at any patient age and as a secondary repair for any given primary type of cleft disorder.