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Molly M. Stevens - One of the best experts on this subject based on the ideXlab platform.

  • effect of formulation method Lipid Composition and pegylation on vesicle lamellarity a small angle neutron scattering study
    Langmuir, 2019
    Co-Authors: Valeria Nele, Jj Doutch, Margaret N. Holme, Ulrike Kauscher, Michael R. Thomas, Molly M. Stevens
    Abstract:

    Liposomes are well-established systems for drug delivery and biosensing applications. The design of a liposomal carrier requires careful choice of Lipid Composition and formulation method. These determine many vesicle properties including lamellarity, which can have a strong effect on both encapsulation efficiency and the efflux rate of encapsulated active compounds. Despite this, a comprehensive study on how the Lipid Composition and formulation method affect vesicle lamellarity is still lacking. Here, we combine small-angle neutron scattering and cryogenic transmission electron microscopy to study the effect of three different well-established formulation methods followed by extrusion through 100 nm polycarbonate membranes on the resulting vesicle membrane structure. Specifically, we examine vesicles formulated from the commonly used phosphoLipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC...

  • Effect of Formulation Method, Lipid Composition, and PEGylation on Vesicle Lamellarity: A Small-Angle Neutron Scattering Study
    2019
    Co-Authors: Valeria Nele, Jj Doutch, Margaret N. Holme, Ulrike Kauscher, Michael R. Thomas, Molly M. Stevens
    Abstract:

    Liposomes are well-established systems for drug delivery and biosensing applications. The design of a liposomal carrier requires careful choice of Lipid Composition and formulation method. These determine many vesicle properties including lamellarity, which can have a strong effect on both encapsulation efficiency and the efflux rate of encapsulated active compounds. Despite this, a comprehensive study on how the Lipid Composition and formulation method affect vesicle lamellarity is still lacking. Here, we combine small-angle neutron scattering and cryogenic transmission electron microscopy to study the effect of three different well-established formulation methods followed by extrusion through 100 nm polycarbonate membranes on the resulting vesicle membrane structure. Specifically, we examine vesicles formulated from the commonly used phosphoLipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) via film hydration followed by (i) agitation on a shaker or (ii) freeze–thawing, or (iii) the reverse-phase evaporation vesicle method. After extrusion, up to half of the total Lipid content is still assembled into multilamellar structures. However, we achieved unilamellar vesicle populations when as little as 0.1 mol % PEG-modified Lipid was included in the vesicle formulation. Interestingly, DPPC with 5 mol % PEGylated Lipid produces a combination of cylindrical micelles and vesicles. In conclusion, our results provide important insights into the effect of the formulation method and Lipid Composition on producing liposomes with a defined membrane structure

Valeria Nele - One of the best experts on this subject based on the ideXlab platform.

  • effect of formulation method Lipid Composition and pegylation on vesicle lamellarity a small angle neutron scattering study
    Langmuir, 2019
    Co-Authors: Valeria Nele, Jj Doutch, Margaret N. Holme, Ulrike Kauscher, Michael R. Thomas, Molly M. Stevens
    Abstract:

    Liposomes are well-established systems for drug delivery and biosensing applications. The design of a liposomal carrier requires careful choice of Lipid Composition and formulation method. These determine many vesicle properties including lamellarity, which can have a strong effect on both encapsulation efficiency and the efflux rate of encapsulated active compounds. Despite this, a comprehensive study on how the Lipid Composition and formulation method affect vesicle lamellarity is still lacking. Here, we combine small-angle neutron scattering and cryogenic transmission electron microscopy to study the effect of three different well-established formulation methods followed by extrusion through 100 nm polycarbonate membranes on the resulting vesicle membrane structure. Specifically, we examine vesicles formulated from the commonly used phosphoLipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC...

  • Effect of Formulation Method, Lipid Composition, and PEGylation on Vesicle Lamellarity: A Small-Angle Neutron Scattering Study
    2019
    Co-Authors: Valeria Nele, Jj Doutch, Margaret N. Holme, Ulrike Kauscher, Michael R. Thomas, Molly M. Stevens
    Abstract:

    Liposomes are well-established systems for drug delivery and biosensing applications. The design of a liposomal carrier requires careful choice of Lipid Composition and formulation method. These determine many vesicle properties including lamellarity, which can have a strong effect on both encapsulation efficiency and the efflux rate of encapsulated active compounds. Despite this, a comprehensive study on how the Lipid Composition and formulation method affect vesicle lamellarity is still lacking. Here, we combine small-angle neutron scattering and cryogenic transmission electron microscopy to study the effect of three different well-established formulation methods followed by extrusion through 100 nm polycarbonate membranes on the resulting vesicle membrane structure. Specifically, we examine vesicles formulated from the commonly used phosphoLipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) via film hydration followed by (i) agitation on a shaker or (ii) freeze–thawing, or (iii) the reverse-phase evaporation vesicle method. After extrusion, up to half of the total Lipid content is still assembled into multilamellar structures. However, we achieved unilamellar vesicle populations when as little as 0.1 mol % PEG-modified Lipid was included in the vesicle formulation. Interestingly, DPPC with 5 mol % PEGylated Lipid produces a combination of cylindrical micelles and vesicles. In conclusion, our results provide important insights into the effect of the formulation method and Lipid Composition on producing liposomes with a defined membrane structure

Jeroen Van Smeden - One of the best experts on this subject based on the ideXlab platform.

  • altered expression of epidermal Lipid bio synthesis enzymes in atopic dermatitis skin is accompanied by changes in stratum corneum Lipid Composition
    Journal of Dermatological Science, 2017
    Co-Authors: Mogbekeloluwa O Danso, Walter Boiten, Vincent Van Drongelen, Rob J Vreeken, Kevin Gmelig Meijling, Gert S Gooris, Abdoel Waheb El Ghalbzouri, Samira Absalah, Sanja Kezic, Jeroen Van Smeden
    Abstract:

    Abstract Background The barrier dysfunction in atopic dermatitis (AD) skin correlates with stratum corneum (SC) Lipid abnormalities including reduction of global Lipid content, shorter ceramide (CER) as well as free fatty acid (FFA) chain length and altered CER subclass levels. However, the underlying cause of these changes in Lipid Composition has not been fully investigated. Aim We investigated whether the expression of CER and FFA biosynthesis enzymes are altered in AD skin compared with control skin and determine whether changes in enzyme expression can be related with changes in Lipid Composition. Methods In AD patients and controls the expression of enzymes involved in the biosynthesis of FFAs and CERs was analyzed in relation to the SC Lipid Composition. These enzymes include stearoyl CoA desaturase (SCD), elongase 1 (ELOVL1) and ELOVL6 involved in FFA synthesis and β-glucocerebrosidase (GBA), acid-sphingomyelinase (aSmase), ceramide synthase 3 (CerS3) involved in CER synthesis. In TH2 treated human skin equivalents (AD HSEs) mimicking lesional AD skin, the mRNA expression of these enzymes was investigated. Results The results reveal an altered expression of SCD and ELOVL1 in AD lesional skin. This was accompanied by functional changes displayed by increased unsaturated FFAs (SCD) and reduced FFA C22-C28 (ELOVL1) in AD lesional skin. The expression of GBA, aSmase and CerS3 were also altered in lesional skin. The CER Composition in AD lesional skin showed corresponding changes such as increased CER AS and NS (aSmase) and decreased esterified ω-hydroxy CERs (CerS3). In support of the results from AD skin, the AD HSEs showed reduced mRNA ELOVL1, GBA and a Smase levels. Conclusion This study shows that alterations in the expression of key enzymes involved in SC Lipid synthesis contribute to changes in the Lipid Composition in AD skin and inflammation may influence expression of these enzymes.

  • intercellular skin barrier Lipid Composition and organization in netherton syndrome patients
    Journal of Investigative Dermatology, 2014
    Co-Authors: Jeroen Van Smeden, Alain Hovnanian, Laetitia Furio, Michelle Janssens, Walter Boiten, Vincent Van Drongelen, Rob J Vreeken
    Abstract:

    Netherton syndrome (NTS) is a rare genetic skin disease caused by mutations in the serine protease inhibitor Kazal-type 5 gene, which encodes the lympho-epithelial Kazal-type–related inhibitor. NTS patients have profoundly impaired skin barrier function. As stratum corneum (SC) Lipids have a crucial role in the skin barrier function, we investigated the SC Lipid Composition and organization in NTS patients. We studied the SC Lipid Composition by means of mass spectrometry, and the Lipid organization was examined by infrared spectroscopy and X-ray diffraction. Decreased free fatty acid (FFA) chain length and increased levels of monounsaturated FFAs were observed in the SC of NTS patients compared with controls. Furthermore, the level of short-chain ceramides (CERs) was enhanced in NTS patients and a strong reduction in long-chain CER levels was seen in several patients. The changes in Lipid Composition modified the Lipid organization leading to an increased disordering of the Lipids compared with the controls. In addition, in a subgroup of patients the organization of the Lipid layers changed dramatically. The altered FFA and CER profiles in NTS patients corresponded to changes in the expression of enzymes involved in SC Lipid processing. The observed changes in Lipid Composition, Lipid organization, and enzyme expression are likely to contribute to the barrier dysfunction in NTS.

Nicholas C Harvey - One of the best experts on this subject based on the ideXlab platform.

  • placental polar Lipid Composition is associated with placental gene expression and neonatal body Composition
    Biochimica et Biophysica Acta, 2021
    Co-Authors: Olaf Uhl, Rohan M Lewis, Birgit Hirschmugl, S R Crozier, Hazel Inskip, Antonio Gazquez, Nicholas C Harvey
    Abstract:

    The polar-Lipid Composition of the placenta reflects its cellular heterogeneity and metabolism. This study explored relationships between placental polar-Lipid Composition, gene expression and neonatal body Composition. Placental tissue and maternal and offspring data were collected in the Southampton Women's Survey. Lipid and RNA were extracted from placental tissue and polar Lipids measured by mass spectrometry, while gene expression was assessed using the nCounter analysis platform. Principal component analysis was used to identify patterns within placental Lipid Composition and these were correlated with neonatal body Composition and placental gene expression. In the analysis of placental Lipids, the first three principal components explained 19.1%, 12.7% and 8.0% of variation in placental Lipid Composition, respectively. Principal component 2 was characterised by high principal component scores for acyl-alkyl-glycerophosphatidylcholines and Lipid species containing DHA. Principal component 2 was associated with placental weight and neonatal lean mass; this component was associated with gene expression of APOE, PLIN2, FATP2, FABP4, LEP, G0S2, PNPLA2 and SRB1. Principal components 1 and 3 were not related to birth outcomes but they were associated with the gene expression of Lipid related genes. Principal component 1 was associated with expression of LEP, APOE, FATP2 and ACAT2. Principal component 3 was associated with expression of PLIN2, PLIN3 and PNPLA2. This study demonstrates that placentas of different sizes have specific differences in polar-Lipid Composition and related gene expression. These differences in Lipid Composition were associated with birth weight and neonatal lean mass, suggesting that placental Lipid Composition may influence prenatal lean mass accretion.

Jj Doutch - One of the best experts on this subject based on the ideXlab platform.

  • effect of formulation method Lipid Composition and pegylation on vesicle lamellarity a small angle neutron scattering study
    Langmuir, 2019
    Co-Authors: Valeria Nele, Jj Doutch, Margaret N. Holme, Ulrike Kauscher, Michael R. Thomas, Molly M. Stevens
    Abstract:

    Liposomes are well-established systems for drug delivery and biosensing applications. The design of a liposomal carrier requires careful choice of Lipid Composition and formulation method. These determine many vesicle properties including lamellarity, which can have a strong effect on both encapsulation efficiency and the efflux rate of encapsulated active compounds. Despite this, a comprehensive study on how the Lipid Composition and formulation method affect vesicle lamellarity is still lacking. Here, we combine small-angle neutron scattering and cryogenic transmission electron microscopy to study the effect of three different well-established formulation methods followed by extrusion through 100 nm polycarbonate membranes on the resulting vesicle membrane structure. Specifically, we examine vesicles formulated from the commonly used phosphoLipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC...

  • Effect of formulation method, Lipid Composition and PEGylation on vesicle lamellarity: a small-angle neutron scattering study
    'American Chemical Society (ACS)', 2019
    Co-Authors: Holme M, Kauscher U, Thomas Mr, Jj Doutch, Stevens M
    Abstract:

    Liposomes are well-established systems for drug delivery and biosensing applications. The design of a liposomal carrier requires careful choice of Lipid Composition and formulation method. These determine many vesicle properties including lamellarity, which can have a strong effect on both encapsulation efficiency and the efflux rate of encapsulated active compounds. Despite this, a comprehensive study on how the Lipid Composition and formulation method affect vesicle lamellarity is still lacking. Here, we combine small-angle neutron scattering and cryogenic transmission electron microscopy to study the effect of three different well-established formulation methods followed by extrusion through 100 nm polycarbonate membranes on the resulting vesicle membrane structure. Specifically, we examine vesicles formulated from the commonly used phosphoLipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) via film hydration followed by (i) agitation on a shaker or (ii) freeze–thawing, or (iii) the reverse-phase evaporation vesicle method. After extrusion, up to half of the total Lipid content is still assembled into multilamellar structures. However, we achieved unilamellar vesicle populations when as little as 0.1 mol % PEG-modified Lipid was included in the vesicle formulation. Interestingly, DPPC with 5 mol % PEGylated Lipid produces a combination of cylindrical micelles and vesicles. In conclusion, our results provide important insights into the effect of the formulation method and Lipid Composition on producing liposomes with a defined membrane structure

  • Effect of Formulation Method, Lipid Composition, and PEGylation on Vesicle Lamellarity: A Small-Angle Neutron Scattering Study
    2019
    Co-Authors: Valeria Nele, Jj Doutch, Margaret N. Holme, Ulrike Kauscher, Michael R. Thomas, Molly M. Stevens
    Abstract:

    Liposomes are well-established systems for drug delivery and biosensing applications. The design of a liposomal carrier requires careful choice of Lipid Composition and formulation method. These determine many vesicle properties including lamellarity, which can have a strong effect on both encapsulation efficiency and the efflux rate of encapsulated active compounds. Despite this, a comprehensive study on how the Lipid Composition and formulation method affect vesicle lamellarity is still lacking. Here, we combine small-angle neutron scattering and cryogenic transmission electron microscopy to study the effect of three different well-established formulation methods followed by extrusion through 100 nm polycarbonate membranes on the resulting vesicle membrane structure. Specifically, we examine vesicles formulated from the commonly used phosphoLipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) via film hydration followed by (i) agitation on a shaker or (ii) freeze–thawing, or (iii) the reverse-phase evaporation vesicle method. After extrusion, up to half of the total Lipid content is still assembled into multilamellar structures. However, we achieved unilamellar vesicle populations when as little as 0.1 mol % PEG-modified Lipid was included in the vesicle formulation. Interestingly, DPPC with 5 mol % PEGylated Lipid produces a combination of cylindrical micelles and vesicles. In conclusion, our results provide important insights into the effect of the formulation method and Lipid Composition on producing liposomes with a defined membrane structure