The Experts below are selected from a list of 867 Experts worldwide ranked by ideXlab platform
Tony F. Cruz - One of the best experts on this subject based on the ideXlab platform.
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Lipocortin 2 (annexin 2) is a major substrate for constitutive tyrosine kinase activity in chondrocytes
Biochemistry, 1994Co-Authors: Daniel T. Grima, Rita A. Kandel, Pepinsky R Blake, Tony F. CruzAbstract:Treatment of cultured bovine articular chondrocytes with 100 microM orthovanadate, in the absence of serum, results in the production of a single major tyrosine phosphorylated protein with an apparent molecular mass of 36 kDa (p36). Chondrocytes were found to contain proteins reactive with anti-Lipocortin 1, 2, and 5 antibodies. p36 comigrated on SDS-polyacrylamide gels with Lipocortin 2, but not with other members of the Lipocortin family. The distribution of p36 between the particulate and soluble cell fractions was also similar to that of Lipocortin 2. p36 that was purified on an anti-phosphotyrosine immunoaffinity column cross-reacted with anti-Lipocortin 2 antibodies. Similarly, Lipocortin 2 purified on an anti-Lipocortin 2 immunoaffinity column reacted with anti-phosphotyrosine antibodies. Furthermore, cyanogen bromide cleavage fragments of purified Lipocortin 2 and p36 were similar. These data demonstrate that the major constitutively tyrosine phosphorylated protein, in chondrocytes, is Lipocortin 2. Tyrosine phosphorylated p36 required SDS buffers for extraction due to a loss of the tyrosine phosphate group under other solubilization conditions using Triton X-100 or sodium cholate. This study provides a system for the study of the effects of tyrosine phosphorylation on Lipocortin 2 function. What role Lipocortin 2 plays in chondrocyte biology remains to be determined.
Mark A Israel - One of the best experts on this subject based on the ideXlab platform.
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developmental regulation of annexin ii Lipocortin 2 in human brain and expression in high grade glioma
Cancer Research, 1992Co-Authors: Steven A Reeves, Cindy Chavezkappel, Richard J Davis, Mark L Rosenblum, Mark A IsraelAbstract:In experiments to identify molecules that might be important in the pathogenesis of glioblastoma multiforme, the most common malignant brain tumor, we found that annexin II (Lipocortin 2, p36), a likely second messenger in several different mitogenic pathways, was highly expressed in tumor tissue of glioblastoma multiforme (9 of 9) and highly anaplastic astrocytoma (2 of 6), but not in astrocytomas of lower pathological grade (0 of 6). We also detected high levels of annexin II expression in fetal brain during the period when radial glia proliferate, although annexin II expression was not detected in normal adult brain. These data demonstrate that annexin II expression is developmentally regulated in the human central nervous system and suggest that the early progenitor radial glia share important characteristics with highly malignant glial tumors.
Daniel T. Grima - One of the best experts on this subject based on the ideXlab platform.
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Lipocortin 2 (annexin 2) is a major substrate for constitutive tyrosine kinase activity in chondrocytes
Biochemistry, 1994Co-Authors: Daniel T. Grima, Rita A. Kandel, Pepinsky R Blake, Tony F. CruzAbstract:Treatment of cultured bovine articular chondrocytes with 100 microM orthovanadate, in the absence of serum, results in the production of a single major tyrosine phosphorylated protein with an apparent molecular mass of 36 kDa (p36). Chondrocytes were found to contain proteins reactive with anti-Lipocortin 1, 2, and 5 antibodies. p36 comigrated on SDS-polyacrylamide gels with Lipocortin 2, but not with other members of the Lipocortin family. The distribution of p36 between the particulate and soluble cell fractions was also similar to that of Lipocortin 2. p36 that was purified on an anti-phosphotyrosine immunoaffinity column cross-reacted with anti-Lipocortin 2 antibodies. Similarly, Lipocortin 2 purified on an anti-Lipocortin 2 immunoaffinity column reacted with anti-phosphotyrosine antibodies. Furthermore, cyanogen bromide cleavage fragments of purified Lipocortin 2 and p36 were similar. These data demonstrate that the major constitutively tyrosine phosphorylated protein, in chondrocytes, is Lipocortin 2. Tyrosine phosphorylated p36 required SDS buffers for extraction due to a loss of the tyrosine phosphate group under other solubilization conditions using Triton X-100 or sodium cholate. This study provides a system for the study of the effects of tyrosine phosphorylation on Lipocortin 2 function. What role Lipocortin 2 plays in chondrocyte biology remains to be determined.
Steven A Reeves - One of the best experts on this subject based on the ideXlab platform.
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developmental regulation of annexin ii Lipocortin 2 in human brain and expression in high grade glioma
Cancer Research, 1992Co-Authors: Steven A Reeves, Cindy Chavezkappel, Richard J Davis, Mark L Rosenblum, Mark A IsraelAbstract:In experiments to identify molecules that might be important in the pathogenesis of glioblastoma multiforme, the most common malignant brain tumor, we found that annexin II (Lipocortin 2, p36), a likely second messenger in several different mitogenic pathways, was highly expressed in tumor tissue of glioblastoma multiforme (9 of 9) and highly anaplastic astrocytoma (2 of 6), but not in astrocytomas of lower pathological grade (0 of 6). We also detected high levels of annexin II expression in fetal brain during the period when radial glia proliferate, although annexin II expression was not detected in normal adult brain. These data demonstrate that annexin II expression is developmentally regulated in the human central nervous system and suggest that the early progenitor radial glia share important characteristics with highly malignant glial tumors.
Rita A. Kandel - One of the best experts on this subject based on the ideXlab platform.
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Lipocortin 2 (annexin 2) is a major substrate for constitutive tyrosine kinase activity in chondrocytes
Biochemistry, 1994Co-Authors: Daniel T. Grima, Rita A. Kandel, Pepinsky R Blake, Tony F. CruzAbstract:Treatment of cultured bovine articular chondrocytes with 100 microM orthovanadate, in the absence of serum, results in the production of a single major tyrosine phosphorylated protein with an apparent molecular mass of 36 kDa (p36). Chondrocytes were found to contain proteins reactive with anti-Lipocortin 1, 2, and 5 antibodies. p36 comigrated on SDS-polyacrylamide gels with Lipocortin 2, but not with other members of the Lipocortin family. The distribution of p36 between the particulate and soluble cell fractions was also similar to that of Lipocortin 2. p36 that was purified on an anti-phosphotyrosine immunoaffinity column cross-reacted with anti-Lipocortin 2 antibodies. Similarly, Lipocortin 2 purified on an anti-Lipocortin 2 immunoaffinity column reacted with anti-phosphotyrosine antibodies. Furthermore, cyanogen bromide cleavage fragments of purified Lipocortin 2 and p36 were similar. These data demonstrate that the major constitutively tyrosine phosphorylated protein, in chondrocytes, is Lipocortin 2. Tyrosine phosphorylated p36 required SDS buffers for extraction due to a loss of the tyrosine phosphate group under other solubilization conditions using Triton X-100 or sodium cholate. This study provides a system for the study of the effects of tyrosine phosphorylation on Lipocortin 2 function. What role Lipocortin 2 plays in chondrocyte biology remains to be determined.