The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Walter Heuwieser - One of the best experts on this subject based on the ideXlab platform.
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determination of ceftiofur derivatives in serum endometrial tissue and Lochia in puerperal dairy cows with fever or acute puerperal metritis after subcutaneous administration of ceftiofur crystalline free acid
Journal of Dairy Science, 2013Co-Authors: X Von Krueger, Peter Scherpenisse, S Roiger, Walter HeuwieserAbstract:Abstract Acute puerperal metritis (APM) is one of the most common diseases during the puerperal period. Systemic administration of ceftiofur for 5 consecutive days has been shown to be effective for treatment of APM. The objective of this study was to determine concentrations of ceftiofur derivatives in serum, endometrial tissue, and Lochia of cows with fever postpartum or APM 4 to 6d after treatment with a single subcutaneous dose of 6.6mg of ceftiofur crystalline free acid (CCFA)/kg of estimated BW at the base of the ear. In the first experiment, samples from CCFA-treated cows with fever postpartum or APM (n=42) were taken on d 4, 5, or 6 after treatment. Concentrations of ceftiofur derivatives were quantified using an HPLC assay. Concentrations of active ceftiofur metabolite desfuroylceftiofuracetamide (DCA) were greatest at d 4 after treatment with CCFA in all samples, but they were considerably lower than the concentrations of DCA in healthy postpartum cows treated with the same dose of CCFA. The concentrations of DCA in serum, endometrial tissue, and Lochia were affected by odor of vaginal discharge before treatment with CCFA. Mean concentrations of DCA could be detected above the reported minimal drug concentrations (minimum inhibitory concentrations, MIC) required to inhibit relevant pathogens such as Escherichia coli and Arcanobacterium pyogenes in serum on all days and in endometrial tissue and Lochia only on d 4 in CCFA-treated cows with fetid vaginal discharge before treatment. In the second experiment, samples from CCFA-treated cows with APM (n=8) were taken on d 0 (before treatment) and d 4, 5, and 6 after treatment. Mean concentrations of DCA in serum and Lochia were similar on d 4 to 6 in both laboratories. Furthermore, determined concentrations of DCA from both laboratories were correlated for serum and Lochia. Mean concentrations of DCA could be detected above the reported MIC in serum and Lochia only on d 4. Our 2 experiments demonstrated that in postpartum cows with fever postpartum or APM concentrations above the MIC for relevant bacteria (>0.5μg/mL or >0.5μg/g) of DCA could be sustained only for 4 (serum: 15/17; endometrial tissue: 2/17; Lochia: 1/16) to 5d (serum: 10/13; endometrial tissue: 1/13; Lochia: 2/12) after a single treatment with CCFA only in a certain proportion of cows. Overall, our data provide first pharmacological evidence that a single subcutaneous administration of 6.6g of CCFA/kg of BW might not be sufficient to efficaciously treat APM in postpartum dairy cows.
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Determination of ceftiofur derivatives in serum, endometrial tissue, and Lochia in puerperal dairy cows after subcutaneous administration of ceftiofur crystalline free acid
Journal of dairy science, 2011Co-Authors: T.s. Witte, Peter Scherpenisse, Aldert A Bergwerff, Michael Iwersen, T Kaufmann, Walter HeuwieserAbstract:Abstract Puerperal uterine infections are often associated with decreased reproductive performance in dairy cows. Routine treatment protocols include the systemic administration of antibiotics. Antibiotic drugs, however, should be administered daily over at least 5 d. The objective of this study was to determine concentrations of ceftiofur derivatives in serum, endometrial tissue, and Lochia after subcutaneous administration of ceftiofur crystalline free acid in 6 clinically healthy puerperal dairy cows with normal parturition. Samples were taken immediately before treatment, 2h after, and then every 24h over a 7-d period. Concentrations of ceftiofur derivatives were quantified using an HPLC assay. In serum and endometrial tissue, ceftiofur derivatives could be detected above the reported minimum drug concentrations required to inhibit relevant pathogens such as Escherichia coli and Arcanobacterium pyogenes over a 7-d period. Concentrations of desfuroylceftiofuracetamide at 5 d after administration of ceftiofur crystalline free acid were 1.21±0.61 μg/mL in serum, 0.86±0.61 μg/mg in endometrial tissue, and 0.96±1.15 μg/mL in Lochia. In Lochia, mean concentrations of ceftiofur derivatives also remained above the minimal inhibitory concentration of relevant pathogens, but showed greater variations between cows.
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ceftiofur derivatives in serum uterine tissues cotyledons and Lochia after fetal membrane retention
Journal of Dairy Science, 2006Co-Authors: M Drillich, Sabina Kersting, Peter Scherpenisse, Sebastian Arlt, Aldert A Bergwerff, Walter HeuwieserAbstract:Abstract The objective of the study was to determine concentrations of ceftiofur derivatives after subcutaneous application of ceftiofur hydrochloride in cows with retained fetal membranes. Concentrations of ceftiofur derivatives detected as desfuroylceftiofuracetamide were determined in blood serum, endometrium, caruncles, cotyledons, and Lochia during 72h. After induction of parturition, 2 primiparous and 4 multiparous cows having retained fetal membranes for at least 12h were studied. All cows received 3 consecutive injections (C1 to C3; 24h apart) of 1-mg ceftiofur equivalents per kilogram of body weight as ceftiofur hydrochloride sterile suspension. Samples of blood, endometrium, caruncles, cotyledons, and Lochia were collected immediately before each injection (0h) and again at 4, 12, and 24h after C1, C2, and C3. Blood samples were collected from coccygeal vessels. Caruncles were removed from the uterine lumen by manual extirpation and separated from cotyledons. Endometrial tissue (0.5g) was collected by using Kenny's biopsy apparatus. For all samples, concentrations of potentially active ceftiofur derivatives were quantified using an HPLC assay. Within 2h (serum), 4h (endometrium), and 12h (caruncles, cotyledons, Lochia) after C1 and during the entire study period, mean concentration of ceftiofur derivatives exceeded the reported minimum drug concentrations required to inhibit the growth of 90% of isolates for relevant bacteria such as Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes. Only in single samples did concentrations decrease temporarily below the reported minimum drug concentrations required to inhibit the growth of 90% of isolates.
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ceftiofur derivatives in serum uterine tissues cotyledons and Lochia after fetal membrane retention
Journal of Dairy Science, 2006Co-Authors: M Drillich, Sabina Kersting, Peter Scherpenisse, Sebastian Arlt, Aldert A Bergwerff, Walter HeuwieserAbstract:Abstract The objective of the study was to determine concentrations of ceftiofur derivatives after subcutaneous application of ceftiofur hydrochloride in cows with retained fetal membranes. Concentrations of ceftiofur derivatives detected as desfuroylceftiofuracetamide were determined in blood serum, endometrium, caruncles, cotyledons, and Lochia during 72h. After induction of parturition, 2 primiparous and 4 multiparous cows having retained fetal membranes for at least 12h were studied. All cows received 3 consecutive injections (C1 to C3; 24h apart) of 1-mg ceftiofur equivalents per kilogram of body weight as ceftiofur hydrochloride sterile suspension. Samples of blood, endometrium, caruncles, cotyledons, and Lochia were collected immediately before each injection (0h) and again at 4, 12, and 24h after C1, C2, and C3. Blood samples were collected from coccygeal vessels. Caruncles were removed from the uterine lumen by manual extirpation and separated from cotyledons. Endometrial tissue (0.5g) was collected by using Kenny's biopsy apparatus. For all samples, concentrations of potentially active ceftiofur derivatives were quantified using an HPLC assay. Within 2h (serum), 4h (endometrium), and 12h (caruncles, cotyledons, Lochia) after C1 and during the entire study period, mean concentration of ceftiofur derivatives exceeded the reported minimum drug concentrations required to inhibit the growth of 90% of isolates for relevant bacteria such as Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes. Only in single samples did concentrations decrease temporarily below the reported minimum drug concentrations required to inhibit the growth of 90% of isolates.
Peter Scherpenisse - One of the best experts on this subject based on the ideXlab platform.
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determination of ceftiofur derivatives in serum endometrial tissue and Lochia in puerperal dairy cows with fever or acute puerperal metritis after subcutaneous administration of ceftiofur crystalline free acid
Journal of Dairy Science, 2013Co-Authors: X Von Krueger, Peter Scherpenisse, S Roiger, Walter HeuwieserAbstract:Abstract Acute puerperal metritis (APM) is one of the most common diseases during the puerperal period. Systemic administration of ceftiofur for 5 consecutive days has been shown to be effective for treatment of APM. The objective of this study was to determine concentrations of ceftiofur derivatives in serum, endometrial tissue, and Lochia of cows with fever postpartum or APM 4 to 6d after treatment with a single subcutaneous dose of 6.6mg of ceftiofur crystalline free acid (CCFA)/kg of estimated BW at the base of the ear. In the first experiment, samples from CCFA-treated cows with fever postpartum or APM (n=42) were taken on d 4, 5, or 6 after treatment. Concentrations of ceftiofur derivatives were quantified using an HPLC assay. Concentrations of active ceftiofur metabolite desfuroylceftiofuracetamide (DCA) were greatest at d 4 after treatment with CCFA in all samples, but they were considerably lower than the concentrations of DCA in healthy postpartum cows treated with the same dose of CCFA. The concentrations of DCA in serum, endometrial tissue, and Lochia were affected by odor of vaginal discharge before treatment with CCFA. Mean concentrations of DCA could be detected above the reported minimal drug concentrations (minimum inhibitory concentrations, MIC) required to inhibit relevant pathogens such as Escherichia coli and Arcanobacterium pyogenes in serum on all days and in endometrial tissue and Lochia only on d 4 in CCFA-treated cows with fetid vaginal discharge before treatment. In the second experiment, samples from CCFA-treated cows with APM (n=8) were taken on d 0 (before treatment) and d 4, 5, and 6 after treatment. Mean concentrations of DCA in serum and Lochia were similar on d 4 to 6 in both laboratories. Furthermore, determined concentrations of DCA from both laboratories were correlated for serum and Lochia. Mean concentrations of DCA could be detected above the reported MIC in serum and Lochia only on d 4. Our 2 experiments demonstrated that in postpartum cows with fever postpartum or APM concentrations above the MIC for relevant bacteria (>0.5μg/mL or >0.5μg/g) of DCA could be sustained only for 4 (serum: 15/17; endometrial tissue: 2/17; Lochia: 1/16) to 5d (serum: 10/13; endometrial tissue: 1/13; Lochia: 2/12) after a single treatment with CCFA only in a certain proportion of cows. Overall, our data provide first pharmacological evidence that a single subcutaneous administration of 6.6g of CCFA/kg of BW might not be sufficient to efficaciously treat APM in postpartum dairy cows.
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Determination of ceftiofur derivatives in serum, endometrial tissue, and Lochia in puerperal dairy cows after subcutaneous administration of ceftiofur crystalline free acid
Journal of dairy science, 2011Co-Authors: T.s. Witte, Peter Scherpenisse, Aldert A Bergwerff, Michael Iwersen, T Kaufmann, Walter HeuwieserAbstract:Abstract Puerperal uterine infections are often associated with decreased reproductive performance in dairy cows. Routine treatment protocols include the systemic administration of antibiotics. Antibiotic drugs, however, should be administered daily over at least 5 d. The objective of this study was to determine concentrations of ceftiofur derivatives in serum, endometrial tissue, and Lochia after subcutaneous administration of ceftiofur crystalline free acid in 6 clinically healthy puerperal dairy cows with normal parturition. Samples were taken immediately before treatment, 2h after, and then every 24h over a 7-d period. Concentrations of ceftiofur derivatives were quantified using an HPLC assay. In serum and endometrial tissue, ceftiofur derivatives could be detected above the reported minimum drug concentrations required to inhibit relevant pathogens such as Escherichia coli and Arcanobacterium pyogenes over a 7-d period. Concentrations of desfuroylceftiofuracetamide at 5 d after administration of ceftiofur crystalline free acid were 1.21±0.61 μg/mL in serum, 0.86±0.61 μg/mg in endometrial tissue, and 0.96±1.15 μg/mL in Lochia. In Lochia, mean concentrations of ceftiofur derivatives also remained above the minimal inhibitory concentration of relevant pathogens, but showed greater variations between cows.
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ceftiofur derivatives in serum uterine tissues cotyledons and Lochia after fetal membrane retention
Journal of Dairy Science, 2006Co-Authors: M Drillich, Sabina Kersting, Peter Scherpenisse, Sebastian Arlt, Aldert A Bergwerff, Walter HeuwieserAbstract:Abstract The objective of the study was to determine concentrations of ceftiofur derivatives after subcutaneous application of ceftiofur hydrochloride in cows with retained fetal membranes. Concentrations of ceftiofur derivatives detected as desfuroylceftiofuracetamide were determined in blood serum, endometrium, caruncles, cotyledons, and Lochia during 72h. After induction of parturition, 2 primiparous and 4 multiparous cows having retained fetal membranes for at least 12h were studied. All cows received 3 consecutive injections (C1 to C3; 24h apart) of 1-mg ceftiofur equivalents per kilogram of body weight as ceftiofur hydrochloride sterile suspension. Samples of blood, endometrium, caruncles, cotyledons, and Lochia were collected immediately before each injection (0h) and again at 4, 12, and 24h after C1, C2, and C3. Blood samples were collected from coccygeal vessels. Caruncles were removed from the uterine lumen by manual extirpation and separated from cotyledons. Endometrial tissue (0.5g) was collected by using Kenny's biopsy apparatus. For all samples, concentrations of potentially active ceftiofur derivatives were quantified using an HPLC assay. Within 2h (serum), 4h (endometrium), and 12h (caruncles, cotyledons, Lochia) after C1 and during the entire study period, mean concentration of ceftiofur derivatives exceeded the reported minimum drug concentrations required to inhibit the growth of 90% of isolates for relevant bacteria such as Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes. Only in single samples did concentrations decrease temporarily below the reported minimum drug concentrations required to inhibit the growth of 90% of isolates.
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ceftiofur derivatives in serum uterine tissues cotyledons and Lochia after fetal membrane retention
Journal of Dairy Science, 2006Co-Authors: M Drillich, Sabina Kersting, Peter Scherpenisse, Sebastian Arlt, Aldert A Bergwerff, Walter HeuwieserAbstract:Abstract The objective of the study was to determine concentrations of ceftiofur derivatives after subcutaneous application of ceftiofur hydrochloride in cows with retained fetal membranes. Concentrations of ceftiofur derivatives detected as desfuroylceftiofuracetamide were determined in blood serum, endometrium, caruncles, cotyledons, and Lochia during 72h. After induction of parturition, 2 primiparous and 4 multiparous cows having retained fetal membranes for at least 12h were studied. All cows received 3 consecutive injections (C1 to C3; 24h apart) of 1-mg ceftiofur equivalents per kilogram of body weight as ceftiofur hydrochloride sterile suspension. Samples of blood, endometrium, caruncles, cotyledons, and Lochia were collected immediately before each injection (0h) and again at 4, 12, and 24h after C1, C2, and C3. Blood samples were collected from coccygeal vessels. Caruncles were removed from the uterine lumen by manual extirpation and separated from cotyledons. Endometrial tissue (0.5g) was collected by using Kenny's biopsy apparatus. For all samples, concentrations of potentially active ceftiofur derivatives were quantified using an HPLC assay. Within 2h (serum), 4h (endometrium), and 12h (caruncles, cotyledons, Lochia) after C1 and during the entire study period, mean concentration of ceftiofur derivatives exceeded the reported minimum drug concentrations required to inhibit the growth of 90% of isolates for relevant bacteria such as Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes. Only in single samples did concentrations decrease temporarily below the reported minimum drug concentrations required to inhibit the growth of 90% of isolates.
Aldert A Bergwerff - One of the best experts on this subject based on the ideXlab platform.
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Determination of ceftiofur derivatives in serum, endometrial tissue, and Lochia in puerperal dairy cows after subcutaneous administration of ceftiofur crystalline free acid
Journal of dairy science, 2011Co-Authors: T.s. Witte, Peter Scherpenisse, Aldert A Bergwerff, Michael Iwersen, T Kaufmann, Walter HeuwieserAbstract:Abstract Puerperal uterine infections are often associated with decreased reproductive performance in dairy cows. Routine treatment protocols include the systemic administration of antibiotics. Antibiotic drugs, however, should be administered daily over at least 5 d. The objective of this study was to determine concentrations of ceftiofur derivatives in serum, endometrial tissue, and Lochia after subcutaneous administration of ceftiofur crystalline free acid in 6 clinically healthy puerperal dairy cows with normal parturition. Samples were taken immediately before treatment, 2h after, and then every 24h over a 7-d period. Concentrations of ceftiofur derivatives were quantified using an HPLC assay. In serum and endometrial tissue, ceftiofur derivatives could be detected above the reported minimum drug concentrations required to inhibit relevant pathogens such as Escherichia coli and Arcanobacterium pyogenes over a 7-d period. Concentrations of desfuroylceftiofuracetamide at 5 d after administration of ceftiofur crystalline free acid were 1.21±0.61 μg/mL in serum, 0.86±0.61 μg/mg in endometrial tissue, and 0.96±1.15 μg/mL in Lochia. In Lochia, mean concentrations of ceftiofur derivatives also remained above the minimal inhibitory concentration of relevant pathogens, but showed greater variations between cows.
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ceftiofur derivatives in serum uterine tissues cotyledons and Lochia after fetal membrane retention
Journal of Dairy Science, 2006Co-Authors: M Drillich, Sabina Kersting, Peter Scherpenisse, Sebastian Arlt, Aldert A Bergwerff, Walter HeuwieserAbstract:Abstract The objective of the study was to determine concentrations of ceftiofur derivatives after subcutaneous application of ceftiofur hydrochloride in cows with retained fetal membranes. Concentrations of ceftiofur derivatives detected as desfuroylceftiofuracetamide were determined in blood serum, endometrium, caruncles, cotyledons, and Lochia during 72h. After induction of parturition, 2 primiparous and 4 multiparous cows having retained fetal membranes for at least 12h were studied. All cows received 3 consecutive injections (C1 to C3; 24h apart) of 1-mg ceftiofur equivalents per kilogram of body weight as ceftiofur hydrochloride sterile suspension. Samples of blood, endometrium, caruncles, cotyledons, and Lochia were collected immediately before each injection (0h) and again at 4, 12, and 24h after C1, C2, and C3. Blood samples were collected from coccygeal vessels. Caruncles were removed from the uterine lumen by manual extirpation and separated from cotyledons. Endometrial tissue (0.5g) was collected by using Kenny's biopsy apparatus. For all samples, concentrations of potentially active ceftiofur derivatives were quantified using an HPLC assay. Within 2h (serum), 4h (endometrium), and 12h (caruncles, cotyledons, Lochia) after C1 and during the entire study period, mean concentration of ceftiofur derivatives exceeded the reported minimum drug concentrations required to inhibit the growth of 90% of isolates for relevant bacteria such as Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes. Only in single samples did concentrations decrease temporarily below the reported minimum drug concentrations required to inhibit the growth of 90% of isolates.
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ceftiofur derivatives in serum uterine tissues cotyledons and Lochia after fetal membrane retention
Journal of Dairy Science, 2006Co-Authors: M Drillich, Sabina Kersting, Peter Scherpenisse, Sebastian Arlt, Aldert A Bergwerff, Walter HeuwieserAbstract:Abstract The objective of the study was to determine concentrations of ceftiofur derivatives after subcutaneous application of ceftiofur hydrochloride in cows with retained fetal membranes. Concentrations of ceftiofur derivatives detected as desfuroylceftiofuracetamide were determined in blood serum, endometrium, caruncles, cotyledons, and Lochia during 72h. After induction of parturition, 2 primiparous and 4 multiparous cows having retained fetal membranes for at least 12h were studied. All cows received 3 consecutive injections (C1 to C3; 24h apart) of 1-mg ceftiofur equivalents per kilogram of body weight as ceftiofur hydrochloride sterile suspension. Samples of blood, endometrium, caruncles, cotyledons, and Lochia were collected immediately before each injection (0h) and again at 4, 12, and 24h after C1, C2, and C3. Blood samples were collected from coccygeal vessels. Caruncles were removed from the uterine lumen by manual extirpation and separated from cotyledons. Endometrial tissue (0.5g) was collected by using Kenny's biopsy apparatus. For all samples, concentrations of potentially active ceftiofur derivatives were quantified using an HPLC assay. Within 2h (serum), 4h (endometrium), and 12h (caruncles, cotyledons, Lochia) after C1 and during the entire study period, mean concentration of ceftiofur derivatives exceeded the reported minimum drug concentrations required to inhibit the growth of 90% of isolates for relevant bacteria such as Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes. Only in single samples did concentrations decrease temporarily below the reported minimum drug concentrations required to inhibit the growth of 90% of isolates.
M Drillich - One of the best experts on this subject based on the ideXlab platform.
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ceftiofur derivatives in serum uterine tissues cotyledons and Lochia after fetal membrane retention
Journal of Dairy Science, 2006Co-Authors: M Drillich, Sabina Kersting, Peter Scherpenisse, Sebastian Arlt, Aldert A Bergwerff, Walter HeuwieserAbstract:Abstract The objective of the study was to determine concentrations of ceftiofur derivatives after subcutaneous application of ceftiofur hydrochloride in cows with retained fetal membranes. Concentrations of ceftiofur derivatives detected as desfuroylceftiofuracetamide were determined in blood serum, endometrium, caruncles, cotyledons, and Lochia during 72h. After induction of parturition, 2 primiparous and 4 multiparous cows having retained fetal membranes for at least 12h were studied. All cows received 3 consecutive injections (C1 to C3; 24h apart) of 1-mg ceftiofur equivalents per kilogram of body weight as ceftiofur hydrochloride sterile suspension. Samples of blood, endometrium, caruncles, cotyledons, and Lochia were collected immediately before each injection (0h) and again at 4, 12, and 24h after C1, C2, and C3. Blood samples were collected from coccygeal vessels. Caruncles were removed from the uterine lumen by manual extirpation and separated from cotyledons. Endometrial tissue (0.5g) was collected by using Kenny's biopsy apparatus. For all samples, concentrations of potentially active ceftiofur derivatives were quantified using an HPLC assay. Within 2h (serum), 4h (endometrium), and 12h (caruncles, cotyledons, Lochia) after C1 and during the entire study period, mean concentration of ceftiofur derivatives exceeded the reported minimum drug concentrations required to inhibit the growth of 90% of isolates for relevant bacteria such as Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes. Only in single samples did concentrations decrease temporarily below the reported minimum drug concentrations required to inhibit the growth of 90% of isolates.
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ceftiofur derivatives in serum uterine tissues cotyledons and Lochia after fetal membrane retention
Journal of Dairy Science, 2006Co-Authors: M Drillich, Sabina Kersting, Peter Scherpenisse, Sebastian Arlt, Aldert A Bergwerff, Walter HeuwieserAbstract:Abstract The objective of the study was to determine concentrations of ceftiofur derivatives after subcutaneous application of ceftiofur hydrochloride in cows with retained fetal membranes. Concentrations of ceftiofur derivatives detected as desfuroylceftiofuracetamide were determined in blood serum, endometrium, caruncles, cotyledons, and Lochia during 72h. After induction of parturition, 2 primiparous and 4 multiparous cows having retained fetal membranes for at least 12h were studied. All cows received 3 consecutive injections (C1 to C3; 24h apart) of 1-mg ceftiofur equivalents per kilogram of body weight as ceftiofur hydrochloride sterile suspension. Samples of blood, endometrium, caruncles, cotyledons, and Lochia were collected immediately before each injection (0h) and again at 4, 12, and 24h after C1, C2, and C3. Blood samples were collected from coccygeal vessels. Caruncles were removed from the uterine lumen by manual extirpation and separated from cotyledons. Endometrial tissue (0.5g) was collected by using Kenny's biopsy apparatus. For all samples, concentrations of potentially active ceftiofur derivatives were quantified using an HPLC assay. Within 2h (serum), 4h (endometrium), and 12h (caruncles, cotyledons, Lochia) after C1 and during the entire study period, mean concentration of ceftiofur derivatives exceeded the reported minimum drug concentrations required to inhibit the growth of 90% of isolates for relevant bacteria such as Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes. Only in single samples did concentrations decrease temporarily below the reported minimum drug concentrations required to inhibit the growth of 90% of isolates.
T.s. Witte - One of the best experts on this subject based on the ideXlab platform.
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Determination of ceftiofur derivatives in serum, endometrial tissue, and Lochia in puerperal dairy cows after subcutaneous administration of ceftiofur crystalline free acid
Journal of dairy science, 2011Co-Authors: T.s. Witte, Peter Scherpenisse, Aldert A Bergwerff, Michael Iwersen, T Kaufmann, Walter HeuwieserAbstract:Abstract Puerperal uterine infections are often associated with decreased reproductive performance in dairy cows. Routine treatment protocols include the systemic administration of antibiotics. Antibiotic drugs, however, should be administered daily over at least 5 d. The objective of this study was to determine concentrations of ceftiofur derivatives in serum, endometrial tissue, and Lochia after subcutaneous administration of ceftiofur crystalline free acid in 6 clinically healthy puerperal dairy cows with normal parturition. Samples were taken immediately before treatment, 2h after, and then every 24h over a 7-d period. Concentrations of ceftiofur derivatives were quantified using an HPLC assay. In serum and endometrial tissue, ceftiofur derivatives could be detected above the reported minimum drug concentrations required to inhibit relevant pathogens such as Escherichia coli and Arcanobacterium pyogenes over a 7-d period. Concentrations of desfuroylceftiofuracetamide at 5 d after administration of ceftiofur crystalline free acid were 1.21±0.61 μg/mL in serum, 0.86±0.61 μg/mg in endometrial tissue, and 0.96±1.15 μg/mL in Lochia. In Lochia, mean concentrations of ceftiofur derivatives also remained above the minimal inhibitory concentration of relevant pathogens, but showed greater variations between cows.
