The Experts below are selected from a list of 43740 Experts worldwide ranked by ideXlab platform
Erkang Wang - One of the best experts on this subject based on the ideXlab platform.
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introducing ratiometric fluorescence to mno2 nanosheet based biosensing a simple label free ratiometric fluorescent sensor programmed by cascade Logic Circuit for ultrasensitive gsh detection
ACS Applied Materials & Interfaces, 2017Co-Authors: Daoqing Fan, Erkang Wang, Changshuai Shang, Shaojun DongAbstract:Glutathione (GSH) plays crucial roles in various bioLogical functions, the level alterations of which have been linked to varieties of diseases. Herein, we for the first time expanded the application of oxidase-like property of MnO2 nanosheet (MnO2 NS) to fluorescent substrates of peroxidase. Different from previously reported fluorescent quenching phenomena, we found that MnO2 NS could not only largely quench the fluorescence of highly fluorescent Scopoletin (SC) but also surprisingly enhance that of nonfluorescent Amplex Red (AR) via oxidation reaction. If MnO2 NS is premixed with GSH, it will be reduced to Mn2+ and lose the oxidase-like property, accompanied by subsequent increase in SC's fluorescence and decrease in AR's. On the basis of the above mechanism, we construct the first MnO2 NS-based ratiometric fluorescent sensor for ultrasensitive and selective detection of GSH. Notably, this ratiometric sensor is programmed by the cascade Logic Circuit (an INHIBIT gate cascade with a 1 to 2 decoder). And a linear relationship between ratiometric fluorescent intensities of the two substrates and logarithmic values of GSH's concentrations is obtained. The detection limit of GSH is as low as 6.7 nM, which is much lower than previous ratiometric fluorescent sensors, and the lowest MnO2 NS-based fluorescent GSH sensor reported so far. Furthermore, this sensor is simple, label-free, and low-cost; it also presents excellent applicability in human serum samples.
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aptamer based sensing platform using three way dna junction driven strand displacement and its application in dna Logic Circuit
Analytical Chemistry, 2014Co-Authors: Jinbo Zhu, Libing Zhang, Shaojun Dong, Zhixue Zhou, Erkang WangAbstract:We proposed a new three-way DNA junction-driven strand displacement mode and fabricated an aptamer-based label-free fluorescent sensing platform on the basis of this mechanism. Assembling the aptamer sequence into the three-way DNA junction makes the platform sensitive to the target of the aptamer. A label-free signal readout method, split G-quadruplex enhanced fluorescence of protoporphyrin IX (PPIX), was used to report the final signal. Here, adenosine triphosphatase (ATP) was taken as a model and detected through this approach, and DNA strand could also be detected by it. The mechanism was investigated by native polyacrylamide gel electrophoresis. Furthermore, on the basis of this molecular platform, we built a Logic Circuit with ATP and DNA strands as input. Aptamer played an important role in mediating the small molecule ATP to tune the DNA Logic gate. Through altering the aptamer sequence, this molecular platform will be sensitive to various stimuli and applied in a wide field.
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four way junction driven dna strand displacement and its application in building majority Logic Circuit
ACS Nano, 2013Co-Authors: Jinbo Zhu, Libing Zhang, Shaojun Dong, Erkang WangAbstract:We introduced a four-way DNA junction-driven toehold-mediated strand displacement method. Separation of the different functional domains on different strands in the four-way junction structure and usage of glue strand to recombine them for different Logic gates make the design more flexible. On the basis of this mechanism, a majority Logic Circuit fabricated by DNA strands was designed and constructed by assembling three AND gates and one OR gate together. The output strand drew the G-rich segments together to form a split G-quadruplex, which could specifically bind PPIX and enhance its fluorescence. Just like a poll with three voters, the high fluorescence signal would be given off only when two or three voters vote in favor. Upon slight modification, the majority Circuit was utilized to select the composite number from 0 to 9 represented by excess-three code. It is a successful attempt to integrate the Logic gates into a Circuit and to achieve desired functions.
Shaojun Dong - One of the best experts on this subject based on the ideXlab platform.
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introducing ratiometric fluorescence to mno2 nanosheet based biosensing a simple label free ratiometric fluorescent sensor programmed by cascade Logic Circuit for ultrasensitive gsh detection
ACS Applied Materials & Interfaces, 2017Co-Authors: Daoqing Fan, Erkang Wang, Changshuai Shang, Shaojun DongAbstract:Glutathione (GSH) plays crucial roles in various bioLogical functions, the level alterations of which have been linked to varieties of diseases. Herein, we for the first time expanded the application of oxidase-like property of MnO2 nanosheet (MnO2 NS) to fluorescent substrates of peroxidase. Different from previously reported fluorescent quenching phenomena, we found that MnO2 NS could not only largely quench the fluorescence of highly fluorescent Scopoletin (SC) but also surprisingly enhance that of nonfluorescent Amplex Red (AR) via oxidation reaction. If MnO2 NS is premixed with GSH, it will be reduced to Mn2+ and lose the oxidase-like property, accompanied by subsequent increase in SC's fluorescence and decrease in AR's. On the basis of the above mechanism, we construct the first MnO2 NS-based ratiometric fluorescent sensor for ultrasensitive and selective detection of GSH. Notably, this ratiometric sensor is programmed by the cascade Logic Circuit (an INHIBIT gate cascade with a 1 to 2 decoder). And a linear relationship between ratiometric fluorescent intensities of the two substrates and logarithmic values of GSH's concentrations is obtained. The detection limit of GSH is as low as 6.7 nM, which is much lower than previous ratiometric fluorescent sensors, and the lowest MnO2 NS-based fluorescent GSH sensor reported so far. Furthermore, this sensor is simple, label-free, and low-cost; it also presents excellent applicability in human serum samples.
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aptamer based sensing platform using three way dna junction driven strand displacement and its application in dna Logic Circuit
Analytical Chemistry, 2014Co-Authors: Jinbo Zhu, Libing Zhang, Shaojun Dong, Zhixue Zhou, Erkang WangAbstract:We proposed a new three-way DNA junction-driven strand displacement mode and fabricated an aptamer-based label-free fluorescent sensing platform on the basis of this mechanism. Assembling the aptamer sequence into the three-way DNA junction makes the platform sensitive to the target of the aptamer. A label-free signal readout method, split G-quadruplex enhanced fluorescence of protoporphyrin IX (PPIX), was used to report the final signal. Here, adenosine triphosphatase (ATP) was taken as a model and detected through this approach, and DNA strand could also be detected by it. The mechanism was investigated by native polyacrylamide gel electrophoresis. Furthermore, on the basis of this molecular platform, we built a Logic Circuit with ATP and DNA strands as input. Aptamer played an important role in mediating the small molecule ATP to tune the DNA Logic gate. Through altering the aptamer sequence, this molecular platform will be sensitive to various stimuli and applied in a wide field.
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four way junction driven dna strand displacement and its application in building majority Logic Circuit
ACS Nano, 2013Co-Authors: Jinbo Zhu, Libing Zhang, Shaojun Dong, Erkang WangAbstract:We introduced a four-way DNA junction-driven toehold-mediated strand displacement method. Separation of the different functional domains on different strands in the four-way junction structure and usage of glue strand to recombine them for different Logic gates make the design more flexible. On the basis of this mechanism, a majority Logic Circuit fabricated by DNA strands was designed and constructed by assembling three AND gates and one OR gate together. The output strand drew the G-rich segments together to form a split G-quadruplex, which could specifically bind PPIX and enhance its fluorescence. Just like a poll with three voters, the high fluorescence signal would be given off only when two or three voters vote in favor. Upon slight modification, the majority Circuit was utilized to select the composite number from 0 to 9 represented by excess-three code. It is a successful attempt to integrate the Logic gates into a Circuit and to achieve desired functions.
Lei Zhang - One of the best experts on this subject based on the ideXlab platform.
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experimental demonstration of a reconfigurable electro optic directed Logic Circuit using cascaded carrier injection micro ring resonators
Scientific Reports, 2017Co-Authors: Yonghui Tia, Zilong Liu, Huifu Xiao, Guoli Zhao, Guipeng Liu, Jianhong Yang, Jianfeng Ding, Lei Zhang, Li YangAbstract:We experimentally demonstrate a reconfigurable electro-optic directed Logic Circuit which can perform any combinatorial Logic operation using cascaded carrier-injection micro-ring resonators (MRRs), and the Logic Circuit is fabricated on the silicon-on-insulator (SOI) substrate with the standard commercial Complementary Metal-Oxide-Semiconductor (CMOS) fabrication process. PIN diodes embedded around MRRs are employed to achieve the carrier injection modulation. The operands are represented by electrical signals, which are applied to the corresponding MRRs to control their switching states. The operation result is directed to the output port in the form of light. For proof of principle, several Logic operations of three-operand with the operation speed of 100 Mbps are demonstrated successfully.
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electro optic directed Logic Circuit based on microring resonators for xor xnor operations
Optics Express, 2012Co-Authors: Lei Zhang, Jianfeng Ding, Yonghui Tian, Lin Yang, Hongtao Chen, Ping Zhou, Weiwei Zhu, Rui MinAbstract:We report the implementation of the XOR and XNOR operations using an electro-optic directed Logic Circuit based on two cascaded silicon microring resonators (MRRs), which are both modulated through the plasma dispersion effect. PIN diodes are embedded around the MRRs to achieve the carrier-injection modulation. The inherent resonance wavelength mismatch between the two nominally identical MRRs caused by fabrication errors is compensated by two local microheaters above each MRR through the thermo-optic effect. Two electrical modulating signals applied to the MRRs represent the two operands of the two operations. Simultaneous bitwise XOR and XNOR operations at 100 Mbit/s are demonstrated.
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proof of concept of directed or nor and and nand Logic Circuit consisting of two parallel microring resonators
Optics Letters, 2011Co-Authors: Yonghui Tian, Jianfeng Ding, Lei Zhang, Lin Yang, Hongtao Chen, Ping Zhou, Weiwei Zhu, Lianxi Jia, Qing FangAbstract:We propose and demonstrate a directed OR/NOR and AND/NAND Logic Circuit consisting of two parallel microring resonators (MRRs). We use two electrical signals representing the two operands of the Logical operation to modulate the two MRRs through the thermo-optic effect, respectively. The final operation results are represented by the output optical signals. Both OR/NOR and AND/NAND operations at 10 kbps are demonstrated.
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simultaneous implementation of xor and xnor operations using a directed Logic Circuit based on two microring resonators
Optics Express, 2011Co-Authors: Lei Zhang, Yonghui Tian, Lin Yang, Ping Zhou, Weiwei Zhu, Lianxi Jia, Yuliang Liu, Qing FangAbstract:We report the simultaneous implementation of the XOR and XNOR operations at two ports of a directed Logic Circuit based on two cascaded microring resonators (MRRs), which are both modulated through thermo-optic effect. Two electrical modulating signals applied to the MRRs represent the two operands of each Logic operation. Simultaneous bitwise XOR and XNOR operations at 10 kbit/s are demonstrated in two different operating modes. We show that such a Circuit can be readily realized using the plasma dispersion effect or the electric field effects, indicating its potential for high-speed operation. We further employ the scattering matrix method to analyze the spectral characteristics of the fabricated Circuit, which can be regarded as a Mach-Zehnder interferometer (MZI) in whole. The two MRRs in the Circuit act as wavelength-dependent splitting and combining units of the MZI. The degradation of the spectra observed in the experiment is found to be related to the length difference between the MZI’s two arms. The evolution of the spectra with this length difference is presented.
Qing Fang - One of the best experts on this subject based on the ideXlab platform.
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proof of concept of directed or nor and and nand Logic Circuit consisting of two parallel microring resonators
Optics Letters, 2011Co-Authors: Yonghui Tian, Jianfeng Ding, Lei Zhang, Lin Yang, Hongtao Chen, Ping Zhou, Weiwei Zhu, Lianxi Jia, Qing FangAbstract:We propose and demonstrate a directed OR/NOR and AND/NAND Logic Circuit consisting of two parallel microring resonators (MRRs). We use two electrical signals representing the two operands of the Logical operation to modulate the two MRRs through the thermo-optic effect, respectively. The final operation results are represented by the output optical signals. Both OR/NOR and AND/NAND operations at 10 kbps are demonstrated.
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simultaneous implementation of xor and xnor operations using a directed Logic Circuit based on two microring resonators
Optics Express, 2011Co-Authors: Lei Zhang, Yonghui Tian, Lin Yang, Ping Zhou, Weiwei Zhu, Lianxi Jia, Yuliang Liu, Qing FangAbstract:We report the simultaneous implementation of the XOR and XNOR operations at two ports of a directed Logic Circuit based on two cascaded microring resonators (MRRs), which are both modulated through thermo-optic effect. Two electrical modulating signals applied to the MRRs represent the two operands of each Logic operation. Simultaneous bitwise XOR and XNOR operations at 10 kbit/s are demonstrated in two different operating modes. We show that such a Circuit can be readily realized using the plasma dispersion effect or the electric field effects, indicating its potential for high-speed operation. We further employ the scattering matrix method to analyze the spectral characteristics of the fabricated Circuit, which can be regarded as a Mach-Zehnder interferometer (MZI) in whole. The two MRRs in the Circuit act as wavelength-dependent splitting and combining units of the MZI. The degradation of the spectra observed in the experiment is found to be related to the length difference between the MZI’s two arms. The evolution of the spectra with this length difference is presented.
Yonghui Tian - One of the best experts on this subject based on the ideXlab platform.
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electro optic directed Logic Circuit based on microring resonators for xor xnor operations
Optics Express, 2012Co-Authors: Lei Zhang, Jianfeng Ding, Yonghui Tian, Lin Yang, Hongtao Chen, Ping Zhou, Weiwei Zhu, Rui MinAbstract:We report the implementation of the XOR and XNOR operations using an electro-optic directed Logic Circuit based on two cascaded silicon microring resonators (MRRs), which are both modulated through the plasma dispersion effect. PIN diodes are embedded around the MRRs to achieve the carrier-injection modulation. The inherent resonance wavelength mismatch between the two nominally identical MRRs caused by fabrication errors is compensated by two local microheaters above each MRR through the thermo-optic effect. Two electrical modulating signals applied to the MRRs represent the two operands of the two operations. Simultaneous bitwise XOR and XNOR operations at 100 Mbit/s are demonstrated.
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proof of concept of directed or nor and and nand Logic Circuit consisting of two parallel microring resonators
Optics Letters, 2011Co-Authors: Yonghui Tian, Jianfeng Ding, Lei Zhang, Lin Yang, Hongtao Chen, Ping Zhou, Weiwei Zhu, Lianxi Jia, Qing FangAbstract:We propose and demonstrate a directed OR/NOR and AND/NAND Logic Circuit consisting of two parallel microring resonators (MRRs). We use two electrical signals representing the two operands of the Logical operation to modulate the two MRRs through the thermo-optic effect, respectively. The final operation results are represented by the output optical signals. Both OR/NOR and AND/NAND operations at 10 kbps are demonstrated.
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simultaneous implementation of xor and xnor operations using a directed Logic Circuit based on two microring resonators
Optics Express, 2011Co-Authors: Lei Zhang, Yonghui Tian, Lin Yang, Ping Zhou, Weiwei Zhu, Lianxi Jia, Yuliang Liu, Qing FangAbstract:We report the simultaneous implementation of the XOR and XNOR operations at two ports of a directed Logic Circuit based on two cascaded microring resonators (MRRs), which are both modulated through thermo-optic effect. Two electrical modulating signals applied to the MRRs represent the two operands of each Logic operation. Simultaneous bitwise XOR and XNOR operations at 10 kbit/s are demonstrated in two different operating modes. We show that such a Circuit can be readily realized using the plasma dispersion effect or the electric field effects, indicating its potential for high-speed operation. We further employ the scattering matrix method to analyze the spectral characteristics of the fabricated Circuit, which can be regarded as a Mach-Zehnder interferometer (MZI) in whole. The two MRRs in the Circuit act as wavelength-dependent splitting and combining units of the MZI. The degradation of the spectra observed in the experiment is found to be related to the length difference between the MZI’s two arms. The evolution of the spectra with this length difference is presented.
