The Experts below are selected from a list of 237117 Experts worldwide ranked by ideXlab platform
Julia Buitink - One of the best experts on this subject based on the ideXlab platform.
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A role for auxin signaling in the acquisition of Longevity during seed maturation
New Phytologist, 2019Co-Authors: Anthoni Pellizzaro, Martine Neveu, David Lalanne, Yuri Kanno, Mitsunori Seo, Olivier Leprince, Julia BuitinkAbstract:1.Seed Longevity, the maintenance of viability during dry storage, is a crucial factor to preserve plant genetic resources and seed vigor. Inference of a temporal gene‐regulatory network of seed maturation identified auxin signaling as a putative mechanism to induce Longevity‐related genes. 2.Using auxin‐response sensors and tryptophan‐dependent auxin biosynthesis mutants of Arabidopsis thaliana L., the role of auxin signaling in Longevity was studied during seed maturation. 3.DII and DR5 sensors demonstrated that concomitant with the acquisition of Longevity, auxin signaling input and output increased and underwent a spatio‐temporal redistribution, spreading throughout the embryo. Longevity of seeds of single auxin biosynthesis mutants with altered auxin signaling activity was affected in a dose‐response manner depending on the level of auxin activity. Longevity‐associated genes with promoters enriched in auxin response elements and the master regulator ABSCISIC ACID INSENSITIVE 3 were induced by auxin in developing embryos and deregulated in auxin biosynthesis mutants. The beneficial effect of exogenous auxin during seed maturation on seed Longevity was abolished in abi3‐1 mutants. 4.These data suggest a role for auxin signaling activity in the acquisition of Longevity during seed maturation.
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Late seed maturation: drying without dying
Journal of Experimental Botany, 2017Co-Authors: Olivier Leprince, Anthoni Pellizzaro, Souha Berriri, Julia BuitinkAbstract:Besides the deposition of storage reserves, seed maturation is characterized by the acquisition of functional traits including germination, desiccation tolerance, dormancy, and Longevity. After seed filling, seed Longevity increases up to 30-fold, concomitant with desiccation that brings the embryo to a quiescent state. The period that we define as late maturation phase can represent 10-78% of total seed development time, yet it remains overlooked. Its importance is underscored by the fact that in the seed production chain, the stage of maturity at harvest is the primary factor that influences seed Longevity and seedling establishment. This review describes the major events and regulatory pathways underlying the acquisition of seed Longevity, focusing on key indicators of maturity such as chlorophyll degradation, accumulation of raffinose family oligosaccharides, late embryogenesis abundant proteins, and heat shock proteins. We discuss how these markers are correlated with or contribute to seed Longevity, and highlight questions that merit further attention. We present evidence suggesting that molecular players involved in biotic defence also have a regulatory role in seed Longevity. We also explore how the concept of plasticity can help understand the acquisition of Longevity.
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Molecular characterization of the acquisition of Longevity during seed maturation in soybean
PLoS ONE, 2017Co-Authors: Juliana Joyce Pereira Lima, David Lalanne, Julia Buitink, Sandra Pelletier, Rubiana Falopa Rossi, Edvaldo Aparecido Amaral Da Silva, Olivier LeprinceAbstract:Seed Longevity, defined as the ability to remain alive during storage, is an important agro-nomic factor. Poor Longevity negatively impacts seedling establishment and consequently crop yield. This is particularly problematic for soybean as seeds have a short lifespan. While the economic importance of soybean has fueled a large number of transcriptome studies during embryogenesis and seed filling, the mechanisms regulating seed Longevity during late maturation remain poorly understood. Here, a detailed physiological and molecular characterization of late seed maturation was performed in soybean to obtain a comprehensive overview of the regulatory genes that are potentially involved in Longevity. Longevity appeared at physiological maturity at the end of seed filling before maturation drying and progressively doubled until the seeds reached the dry state. The increase in Longevity was associated with the expression of genes encoding protective chaperones such as heat shock proteins and the repression of nuclear and chloroplast genes involved in a range of chloroplast activities, including photosynthesis. An increase in the raffinose family oligosac-charides (RFO)/sucrose ratio together with changes in RFO metabolism genes was also associated with Longevity. A gene co-expression network analysis revealed 27 transcription factors whose expression profiles were highly correlated with Longevity. Eight of them were previously identified in the Longevity network of Medicago truncatula, including homologues of ERF110, HSF6AB, NFXL1 and members of the DREB2 family. The network also contained several transcription factors associated with auxin and developmental cell fate during flowering, organ growth and differentiation. A transcriptional transition occurred concomitant with seed chlorophyll loss and detachment from the mother plant, suggesting the activation of a post-abscission program. This transition was enriched with AP2/EREBP and WRKY transcription factors and genes associated with growth, germination and post-transcriptional processes, suggesting that this program prepares the seed for the dry quiescent state and germination.
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Inference of Longevity-Related Genes from a Robust Coexpression Network of Seed Maturation Identifies Regulators Linking Seed Storability to Biotic Defense-Related Pathways
The Plant cell, 2015Co-Authors: Karima Righetti, David Lalanne, Olivier Leprince, Sandra Pelletier, Enrico Glaab, Asher Pasha, Rohan Patel, Nicholas Provart, Jerome Verdier, Julia BuitinkAbstract:Seed Longevity, the maintenance of viability during storage, is a crucial factor for preservation of genetic resources and ensuring proper seedling establishment and high crop yield. We used a systems biology approach to identify key genes regulating the acquisition of Longevity during seed maturation of Medicago truncatula. Using 104 transcriptomes from seed developmental time courses obtained in five growth environments, we generated a robust, stable coexpression network (MatNet), thereby capturing the conserved backbone of maturation. Using a trait-based gene significance measure, a coexpression module related to the acquisition of Longevity was inferred from MatNet. Comparative analysis of the maturation processes in M. truncatula and Arabidopsis thaliana seeds and mining Arabidopsis interaction databases revealed conserved connectivity for 87% of Longevity module nodes between both species. Arabidopsis mutant screening for Longevity and maturation phenotypes demonstrated high predictive power of the Longevity cross-species network. Overrepresentation analysis of the network nodes indicated biological functions related to defense, light, and auxin. Characterization of defense-related wrky3 and nf-x1-like1 (nfxl1) transcription factor mutants demonstrated that these genes regulate some of the network nodes and exhibit impaired acquisition of Longevity during maturation. These data suggest that seed Longevity evolved by co-opting existing genetic pathways regulating the activation of defense against pathogens.
Olivier Leprince - One of the best experts on this subject based on the ideXlab platform.
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A role for auxin signaling in the acquisition of Longevity during seed maturation
New Phytologist, 2019Co-Authors: Anthoni Pellizzaro, Martine Neveu, David Lalanne, Yuri Kanno, Mitsunori Seo, Olivier Leprince, Julia BuitinkAbstract:1.Seed Longevity, the maintenance of viability during dry storage, is a crucial factor to preserve plant genetic resources and seed vigor. Inference of a temporal gene‐regulatory network of seed maturation identified auxin signaling as a putative mechanism to induce Longevity‐related genes. 2.Using auxin‐response sensors and tryptophan‐dependent auxin biosynthesis mutants of Arabidopsis thaliana L., the role of auxin signaling in Longevity was studied during seed maturation. 3.DII and DR5 sensors demonstrated that concomitant with the acquisition of Longevity, auxin signaling input and output increased and underwent a spatio‐temporal redistribution, spreading throughout the embryo. Longevity of seeds of single auxin biosynthesis mutants with altered auxin signaling activity was affected in a dose‐response manner depending on the level of auxin activity. Longevity‐associated genes with promoters enriched in auxin response elements and the master regulator ABSCISIC ACID INSENSITIVE 3 were induced by auxin in developing embryos and deregulated in auxin biosynthesis mutants. The beneficial effect of exogenous auxin during seed maturation on seed Longevity was abolished in abi3‐1 mutants. 4.These data suggest a role for auxin signaling activity in the acquisition of Longevity during seed maturation.
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Late seed maturation: drying without dying
Journal of Experimental Botany, 2017Co-Authors: Olivier Leprince, Anthoni Pellizzaro, Souha Berriri, Julia BuitinkAbstract:Besides the deposition of storage reserves, seed maturation is characterized by the acquisition of functional traits including germination, desiccation tolerance, dormancy, and Longevity. After seed filling, seed Longevity increases up to 30-fold, concomitant with desiccation that brings the embryo to a quiescent state. The period that we define as late maturation phase can represent 10-78% of total seed development time, yet it remains overlooked. Its importance is underscored by the fact that in the seed production chain, the stage of maturity at harvest is the primary factor that influences seed Longevity and seedling establishment. This review describes the major events and regulatory pathways underlying the acquisition of seed Longevity, focusing on key indicators of maturity such as chlorophyll degradation, accumulation of raffinose family oligosaccharides, late embryogenesis abundant proteins, and heat shock proteins. We discuss how these markers are correlated with or contribute to seed Longevity, and highlight questions that merit further attention. We present evidence suggesting that molecular players involved in biotic defence also have a regulatory role in seed Longevity. We also explore how the concept of plasticity can help understand the acquisition of Longevity.
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Molecular characterization of the acquisition of Longevity during seed maturation in soybean
PLoS ONE, 2017Co-Authors: Juliana Joyce Pereira Lima, David Lalanne, Julia Buitink, Sandra Pelletier, Rubiana Falopa Rossi, Edvaldo Aparecido Amaral Da Silva, Olivier LeprinceAbstract:Seed Longevity, defined as the ability to remain alive during storage, is an important agro-nomic factor. Poor Longevity negatively impacts seedling establishment and consequently crop yield. This is particularly problematic for soybean as seeds have a short lifespan. While the economic importance of soybean has fueled a large number of transcriptome studies during embryogenesis and seed filling, the mechanisms regulating seed Longevity during late maturation remain poorly understood. Here, a detailed physiological and molecular characterization of late seed maturation was performed in soybean to obtain a comprehensive overview of the regulatory genes that are potentially involved in Longevity. Longevity appeared at physiological maturity at the end of seed filling before maturation drying and progressively doubled until the seeds reached the dry state. The increase in Longevity was associated with the expression of genes encoding protective chaperones such as heat shock proteins and the repression of nuclear and chloroplast genes involved in a range of chloroplast activities, including photosynthesis. An increase in the raffinose family oligosac-charides (RFO)/sucrose ratio together with changes in RFO metabolism genes was also associated with Longevity. A gene co-expression network analysis revealed 27 transcription factors whose expression profiles were highly correlated with Longevity. Eight of them were previously identified in the Longevity network of Medicago truncatula, including homologues of ERF110, HSF6AB, NFXL1 and members of the DREB2 family. The network also contained several transcription factors associated with auxin and developmental cell fate during flowering, organ growth and differentiation. A transcriptional transition occurred concomitant with seed chlorophyll loss and detachment from the mother plant, suggesting the activation of a post-abscission program. This transition was enriched with AP2/EREBP and WRKY transcription factors and genes associated with growth, germination and post-transcriptional processes, suggesting that this program prepares the seed for the dry quiescent state and germination.
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Inference of Longevity-Related Genes from a Robust Coexpression Network of Seed Maturation Identifies Regulators Linking Seed Storability to Biotic Defense-Related Pathways
The Plant cell, 2015Co-Authors: Karima Righetti, David Lalanne, Olivier Leprince, Sandra Pelletier, Enrico Glaab, Asher Pasha, Rohan Patel, Nicholas Provart, Jerome Verdier, Julia BuitinkAbstract:Seed Longevity, the maintenance of viability during storage, is a crucial factor for preservation of genetic resources and ensuring proper seedling establishment and high crop yield. We used a systems biology approach to identify key genes regulating the acquisition of Longevity during seed maturation of Medicago truncatula. Using 104 transcriptomes from seed developmental time courses obtained in five growth environments, we generated a robust, stable coexpression network (MatNet), thereby capturing the conserved backbone of maturation. Using a trait-based gene significance measure, a coexpression module related to the acquisition of Longevity was inferred from MatNet. Comparative analysis of the maturation processes in M. truncatula and Arabidopsis thaliana seeds and mining Arabidopsis interaction databases revealed conserved connectivity for 87% of Longevity module nodes between both species. Arabidopsis mutant screening for Longevity and maturation phenotypes demonstrated high predictive power of the Longevity cross-species network. Overrepresentation analysis of the network nodes indicated biological functions related to defense, light, and auxin. Characterization of defense-related wrky3 and nf-x1-like1 (nfxl1) transcription factor mutants demonstrated that these genes regulate some of the network nodes and exhibit impaired acquisition of Longevity during maturation. These data suggest that seed Longevity evolved by co-opting existing genetic pathways regulating the activation of defense against pathogens.
David Lalanne - One of the best experts on this subject based on the ideXlab platform.
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A role for auxin signaling in the acquisition of Longevity during seed maturation
New Phytologist, 2019Co-Authors: Anthoni Pellizzaro, Martine Neveu, David Lalanne, Yuri Kanno, Mitsunori Seo, Olivier Leprince, Julia BuitinkAbstract:1.Seed Longevity, the maintenance of viability during dry storage, is a crucial factor to preserve plant genetic resources and seed vigor. Inference of a temporal gene‐regulatory network of seed maturation identified auxin signaling as a putative mechanism to induce Longevity‐related genes. 2.Using auxin‐response sensors and tryptophan‐dependent auxin biosynthesis mutants of Arabidopsis thaliana L., the role of auxin signaling in Longevity was studied during seed maturation. 3.DII and DR5 sensors demonstrated that concomitant with the acquisition of Longevity, auxin signaling input and output increased and underwent a spatio‐temporal redistribution, spreading throughout the embryo. Longevity of seeds of single auxin biosynthesis mutants with altered auxin signaling activity was affected in a dose‐response manner depending on the level of auxin activity. Longevity‐associated genes with promoters enriched in auxin response elements and the master regulator ABSCISIC ACID INSENSITIVE 3 were induced by auxin in developing embryos and deregulated in auxin biosynthesis mutants. The beneficial effect of exogenous auxin during seed maturation on seed Longevity was abolished in abi3‐1 mutants. 4.These data suggest a role for auxin signaling activity in the acquisition of Longevity during seed maturation.
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Molecular characterization of the acquisition of Longevity during seed maturation in soybean
PLoS ONE, 2017Co-Authors: Juliana Joyce Pereira Lima, David Lalanne, Julia Buitink, Sandra Pelletier, Rubiana Falopa Rossi, Edvaldo Aparecido Amaral Da Silva, Olivier LeprinceAbstract:Seed Longevity, defined as the ability to remain alive during storage, is an important agro-nomic factor. Poor Longevity negatively impacts seedling establishment and consequently crop yield. This is particularly problematic for soybean as seeds have a short lifespan. While the economic importance of soybean has fueled a large number of transcriptome studies during embryogenesis and seed filling, the mechanisms regulating seed Longevity during late maturation remain poorly understood. Here, a detailed physiological and molecular characterization of late seed maturation was performed in soybean to obtain a comprehensive overview of the regulatory genes that are potentially involved in Longevity. Longevity appeared at physiological maturity at the end of seed filling before maturation drying and progressively doubled until the seeds reached the dry state. The increase in Longevity was associated with the expression of genes encoding protective chaperones such as heat shock proteins and the repression of nuclear and chloroplast genes involved in a range of chloroplast activities, including photosynthesis. An increase in the raffinose family oligosac-charides (RFO)/sucrose ratio together with changes in RFO metabolism genes was also associated with Longevity. A gene co-expression network analysis revealed 27 transcription factors whose expression profiles were highly correlated with Longevity. Eight of them were previously identified in the Longevity network of Medicago truncatula, including homologues of ERF110, HSF6AB, NFXL1 and members of the DREB2 family. The network also contained several transcription factors associated with auxin and developmental cell fate during flowering, organ growth and differentiation. A transcriptional transition occurred concomitant with seed chlorophyll loss and detachment from the mother plant, suggesting the activation of a post-abscission program. This transition was enriched with AP2/EREBP and WRKY transcription factors and genes associated with growth, germination and post-transcriptional processes, suggesting that this program prepares the seed for the dry quiescent state and germination.
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Inference of Longevity-Related Genes from a Robust Coexpression Network of Seed Maturation Identifies Regulators Linking Seed Storability to Biotic Defense-Related Pathways
The Plant cell, 2015Co-Authors: Karima Righetti, David Lalanne, Olivier Leprince, Sandra Pelletier, Enrico Glaab, Asher Pasha, Rohan Patel, Nicholas Provart, Jerome Verdier, Julia BuitinkAbstract:Seed Longevity, the maintenance of viability during storage, is a crucial factor for preservation of genetic resources and ensuring proper seedling establishment and high crop yield. We used a systems biology approach to identify key genes regulating the acquisition of Longevity during seed maturation of Medicago truncatula. Using 104 transcriptomes from seed developmental time courses obtained in five growth environments, we generated a robust, stable coexpression network (MatNet), thereby capturing the conserved backbone of maturation. Using a trait-based gene significance measure, a coexpression module related to the acquisition of Longevity was inferred from MatNet. Comparative analysis of the maturation processes in M. truncatula and Arabidopsis thaliana seeds and mining Arabidopsis interaction databases revealed conserved connectivity for 87% of Longevity module nodes between both species. Arabidopsis mutant screening for Longevity and maturation phenotypes demonstrated high predictive power of the Longevity cross-species network. Overrepresentation analysis of the network nodes indicated biological functions related to defense, light, and auxin. Characterization of defense-related wrky3 and nf-x1-like1 (nfxl1) transcription factor mutants demonstrated that these genes regulate some of the network nodes and exhibit impaired acquisition of Longevity during maturation. These data suggest that seed Longevity evolved by co-opting existing genetic pathways regulating the activation of defense against pathogens.
Anthoni Pellizzaro - One of the best experts on this subject based on the ideXlab platform.
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A role for auxin signaling in the acquisition of Longevity during seed maturation
New Phytologist, 2019Co-Authors: Anthoni Pellizzaro, Martine Neveu, David Lalanne, Yuri Kanno, Mitsunori Seo, Olivier Leprince, Julia BuitinkAbstract:1.Seed Longevity, the maintenance of viability during dry storage, is a crucial factor to preserve plant genetic resources and seed vigor. Inference of a temporal gene‐regulatory network of seed maturation identified auxin signaling as a putative mechanism to induce Longevity‐related genes. 2.Using auxin‐response sensors and tryptophan‐dependent auxin biosynthesis mutants of Arabidopsis thaliana L., the role of auxin signaling in Longevity was studied during seed maturation. 3.DII and DR5 sensors demonstrated that concomitant with the acquisition of Longevity, auxin signaling input and output increased and underwent a spatio‐temporal redistribution, spreading throughout the embryo. Longevity of seeds of single auxin biosynthesis mutants with altered auxin signaling activity was affected in a dose‐response manner depending on the level of auxin activity. Longevity‐associated genes with promoters enriched in auxin response elements and the master regulator ABSCISIC ACID INSENSITIVE 3 were induced by auxin in developing embryos and deregulated in auxin biosynthesis mutants. The beneficial effect of exogenous auxin during seed maturation on seed Longevity was abolished in abi3‐1 mutants. 4.These data suggest a role for auxin signaling activity in the acquisition of Longevity during seed maturation.
-
Late seed maturation: drying without dying
Journal of Experimental Botany, 2017Co-Authors: Olivier Leprince, Anthoni Pellizzaro, Souha Berriri, Julia BuitinkAbstract:Besides the deposition of storage reserves, seed maturation is characterized by the acquisition of functional traits including germination, desiccation tolerance, dormancy, and Longevity. After seed filling, seed Longevity increases up to 30-fold, concomitant with desiccation that brings the embryo to a quiescent state. The period that we define as late maturation phase can represent 10-78% of total seed development time, yet it remains overlooked. Its importance is underscored by the fact that in the seed production chain, the stage of maturity at harvest is the primary factor that influences seed Longevity and seedling establishment. This review describes the major events and regulatory pathways underlying the acquisition of seed Longevity, focusing on key indicators of maturity such as chlorophyll degradation, accumulation of raffinose family oligosaccharides, late embryogenesis abundant proteins, and heat shock proteins. We discuss how these markers are correlated with or contribute to seed Longevity, and highlight questions that merit further attention. We present evidence suggesting that molecular players involved in biotic defence also have a regulatory role in seed Longevity. We also explore how the concept of plasticity can help understand the acquisition of Longevity.
Sandra Pelletier - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of the acquisition of Longevity during seed maturation in soybean
PLoS ONE, 2017Co-Authors: Juliana Joyce Pereira Lima, David Lalanne, Julia Buitink, Sandra Pelletier, Rubiana Falopa Rossi, Edvaldo Aparecido Amaral Da Silva, Olivier LeprinceAbstract:Seed Longevity, defined as the ability to remain alive during storage, is an important agro-nomic factor. Poor Longevity negatively impacts seedling establishment and consequently crop yield. This is particularly problematic for soybean as seeds have a short lifespan. While the economic importance of soybean has fueled a large number of transcriptome studies during embryogenesis and seed filling, the mechanisms regulating seed Longevity during late maturation remain poorly understood. Here, a detailed physiological and molecular characterization of late seed maturation was performed in soybean to obtain a comprehensive overview of the regulatory genes that are potentially involved in Longevity. Longevity appeared at physiological maturity at the end of seed filling before maturation drying and progressively doubled until the seeds reached the dry state. The increase in Longevity was associated with the expression of genes encoding protective chaperones such as heat shock proteins and the repression of nuclear and chloroplast genes involved in a range of chloroplast activities, including photosynthesis. An increase in the raffinose family oligosac-charides (RFO)/sucrose ratio together with changes in RFO metabolism genes was also associated with Longevity. A gene co-expression network analysis revealed 27 transcription factors whose expression profiles were highly correlated with Longevity. Eight of them were previously identified in the Longevity network of Medicago truncatula, including homologues of ERF110, HSF6AB, NFXL1 and members of the DREB2 family. The network also contained several transcription factors associated with auxin and developmental cell fate during flowering, organ growth and differentiation. A transcriptional transition occurred concomitant with seed chlorophyll loss and detachment from the mother plant, suggesting the activation of a post-abscission program. This transition was enriched with AP2/EREBP and WRKY transcription factors and genes associated with growth, germination and post-transcriptional processes, suggesting that this program prepares the seed for the dry quiescent state and germination.
-
Inference of Longevity-Related Genes from a Robust Coexpression Network of Seed Maturation Identifies Regulators Linking Seed Storability to Biotic Defense-Related Pathways
The Plant cell, 2015Co-Authors: Karima Righetti, David Lalanne, Olivier Leprince, Sandra Pelletier, Enrico Glaab, Asher Pasha, Rohan Patel, Nicholas Provart, Jerome Verdier, Julia BuitinkAbstract:Seed Longevity, the maintenance of viability during storage, is a crucial factor for preservation of genetic resources and ensuring proper seedling establishment and high crop yield. We used a systems biology approach to identify key genes regulating the acquisition of Longevity during seed maturation of Medicago truncatula. Using 104 transcriptomes from seed developmental time courses obtained in five growth environments, we generated a robust, stable coexpression network (MatNet), thereby capturing the conserved backbone of maturation. Using a trait-based gene significance measure, a coexpression module related to the acquisition of Longevity was inferred from MatNet. Comparative analysis of the maturation processes in M. truncatula and Arabidopsis thaliana seeds and mining Arabidopsis interaction databases revealed conserved connectivity for 87% of Longevity module nodes between both species. Arabidopsis mutant screening for Longevity and maturation phenotypes demonstrated high predictive power of the Longevity cross-species network. Overrepresentation analysis of the network nodes indicated biological functions related to defense, light, and auxin. Characterization of defense-related wrky3 and nf-x1-like1 (nfxl1) transcription factor mutants demonstrated that these genes regulate some of the network nodes and exhibit impaired acquisition of Longevity during maturation. These data suggest that seed Longevity evolved by co-opting existing genetic pathways regulating the activation of defense against pathogens.
