The Experts below are selected from a list of 951 Experts worldwide ranked by ideXlab platform
Wei Chen - One of the best experts on this subject based on the ideXlab platform.
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influence of cd2 on growth and chlorophyll fluorescence in a hyperaccumulator Lonicera japonica thunb
Journal of Plant Growth Regulation, 2015Co-Authors: Zhouli Liu, Wei ChenAbstract:Chlorophyll fluorescence is an important instrument to study the responses of plants to cadmium (Cd) stress, which in turn can provide a better understanding of Cd tolerance in plants. In the present study, the influence due to cadmium (Cd2+) exposure on growth and chlorophyll fluorescence was investigated in a new hyperaccumulator—Lonicera japonica Thunb. Four levels of Cd2+ (0, 5, 25, and 125 mg kg−1) were added to the soil. After 90 days of Cd2+ exposure, maximal photochemical efficiency of photosystem II (F v/F m) and effective quantum yield of photosystem II (ΦPS II) of L. japonica showed an increase under 5 mg kg−1 Cd2+ exposure, but decreased under higher concentrations of Cd2+exposure. However, when Cd concentrations in soil were up to the highest concentrations (125 mg kg−1), no significant differences of F v/F m, ΦPS II, photochemical quenching, chlorophyll, and carotenoid contents compared with the control were observed. These results indicate that the good tolerance of L. japonica to Cd might result from effective mechanisms including the capacity to maintain good growth, photosynthetic pigment composition, and chlorophyll fluorescence activity, which would be beneficial to enhance the potential for phytoremediation.
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hormesis effects induced by cadmium on growth and photosynthetic performance in a hyperaccumulator Lonicera japonica thunb
Journal of Plant Growth Regulation, 2015Co-Authors: Lian Jia, Zhouli Liu, Wei ChenAbstract:A soil experiment was designed to investigate the hormesis effect of cadmium (Cd) on the growth and the photosynthetic performance in a hyperaccumulator, Lonicera japonica Thunb. by measuring gas exchange, chlorophyll fluorescence parameters, and photosynthetic pigments. After 90 days of exposure to soil containing 25 mg kg−1 Cd, shoot Cd concentrations reached 168.27 ± 5.01 μg g−1 dry weight, without showing symptoms of visible damage to the plants. The results also show that Cd at low concentrations (≤10 mg kg−1) induced a significant increase in plant biomass, net photosynthetic rate (P n), content of chlorophyll (a, b, and a+b) and carotenoids, effective quantum yield ΦPSII and photochemical quenching coefficient q p, but inhibited them at high concentrations (>25 mg kg−1), confirming a hormetic response. The observed growth increases were closely related to the increase in net photosynthesis induced by Cd, though the causes of the P n increase are still not understood. The present study suggested that hormetic effects should be taken into consideration in phytoremediation of Cd-contaminated soil and the dose range of Cd inducing hormesis on L. japonica is proposed as 2.5–10 mg kg−1 in the soil.
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hormesis phenomena under cd stress in a hyperaccumulator Lonicera japonica thunb
Ecotoxicology, 2013Co-Authors: Lian Jia, Wei Chen, Zhouli Liu, Yanqing HuangAbstract:A hydroponic experiment was carried out to investigate possible hormetic response induced by cadmium (Cd) in a potential hyperaccumulator-Lonicera japonica Thunb. The results showed that Cd at low concentrations induced a significant increase in plant growth, leaf water content and content of photosynthetic pigments in L. japonica, but decreased them at high concentrations, displayed inverted U-shaped dose response curves, confirming a typical biphasic hormetic response. The U-shaped dose response curves were displayed in malondialdehyde (MDA) and electrolyte leakage in leaves at low doses of Cd, indicating reduce oxidative stress and toxic effect. The increase of superoxide dismutase (SOD) and catalase (CAT) activities was observed along with the increased Cd concentration, indicative of increase in anti-oxidative capacity that ensures redox homeostasis is maintained. After 28 days exposure to 10 mg L−1 Cd, stem and leaf Cd concentrations reached 502.96 ± 28.90 and 103.22 ± 5.62 mg kg−1 DW, respectively and the plant had high bioaccumulation coefficient (BC) and translocation factor (TF′). Moreover, the maximum TF value was found at 2.5 mg L−1 Cd treatment, implying that low Cd treatment improved the ability to transfer Cd from medium via roots to aerial structures. Taking together, L. japonica could be considered as a new plant to investigate the underlying mechanisms of hormesis and Cd tolerance. Our results suggest that hormetic effects should be taken into consideration in phytoremediation of Cd-contaminated soil.
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effects of cadmium hyperaccumulation on the concentrations of four trace elements in Lonicera japonica thunb
Ecotoxicology, 2011Co-Authors: Zhouli Liu, Wei ChenAbstract:Hyperaccumulators are important in the phytoremediation of cadmium (Cd)-contaminated soil. In this study, Cd accumulation and the interactions between Cd and four other trace elements (Fe, Mn, Cu, and Zn) in Lonicera japonica Thunb. were investigated. As a result of exposure to soil containing 50 mg kg−1 Cd, stem and shoot Cd concentrations reached 344.49 ± 0.71 and 286.12 ± 9.38 μg g−1 DW respectively, without showing symptoms of visible damage to the plants. This suggests that L. japonica has a strong tolerance to Cd. It is proposed that trace metal elements are involved in the Cd-detoxification mechanisms shown by hyperaccumulators. There is a synergistic interaction in accumulation and translocation between Cd and Fe and a significantly negative correlation between Cd and Cu or Zn concentrations in L. japonica plant tissues. The imbalanced trace element concentrations influences detoxification processes to Cd, therefore, L. japonica could be considered as a new Cd-hyperaccumulator model to investigate the metal tolerance strategies of plants.
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accumulation and tolerance characteristics of cadmium in a potential hyperaccumulator Lonicera japonica thunb
Journal of Hazardous Materials, 2009Co-Authors: Zhouli Liu, Wei Chen, Fenghui Yuan, Kun Yan, Dali TaoAbstract:Phytoremediation using hyperaccumulators is a promising technique of removing soil pollutants. In the study, growth responses, cadmium (Cd) accumulation capability and physiological mechanisms of Lonicera japonica Thunb. under Cd stress were investigated. Exposed to 5 and 10 mg L(-1) Cd, the plants did not show any visual symptoms, furthermore, the height, dry biomass of leaves, roots and total and the chlorophyll (CHL) content were obtained different grade increase. When the concentration of Cd was up to 50 mg L(-1), the height, dry biomass of leaves and roots had not significant differences compared with the control. The indexes of tolerance (IT) were all above 0.8. The maintenance of high superoxide dismutase (SOD) and catalase (CAT) activities was observed along with the increased Cd concentration, suggesting strong internal detoxification mechanisms inside plant cells. After 21 days exposure to 25 mg L(-1) Cd, stem and shoot Cd concentrations reached 344.49+/-0.71 and 286.12+/-9.38 microg g(-1) DW, respectively and the plant had higher bioaccumulation coefficient (BC) and translocation factor (TF). According to these results, it was shown L. japonica had strong tolerance and accumulation capability to Cd, therefore it is a potential Cd-hyperaccumulator.
Xiaobing Chen - One of the best experts on this subject based on the ideXlab platform.
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saline stress enhanced accumulation of leaf phenolics in honeysuckle Lonicera japonica thunb without induction of oxidative stress
Plant Physiology and Biochemistry, 2017Co-Authors: Kun Yan, Shijie Zhao, Lanxing Bian, Xiaobing ChenAbstract:Honeysuckle (Lonicera japonica Thunb.) is a traditional medicinal plant in Chinese, and chlorogenic acid and luteolosid are its specific bioactive phenolic compounds. This study was to investigate leaf antioxidant responses in honeysuckle to saline stress with emphasis on phenolics through hydroponic experiments and field trials. NaCl stress did not stimulate antioxidant system including superoxide dismutase, ascorbate peroxidase, catalase and ascorbate, and had no significant effect on lipid peroxidation in the leaves. Consistently, no inhibition on photochemical capacity of photosystems suggested that reactive oxygen species (ROS) was maintained at a normal level under NaCl stress. However, leaf phenolic synthesis was activated by NaCl stress, indicated by elevated genes transcription and activity of phenylalanine ammonia-lyase and increased phenolics concentration. Specifically, leaf chlorogenic acid concentration was increased by 67.43% and 48.86% after 15 days of 150 and 300 mM NaCl stress, and the increase of luteolosid concentration was 54.26% and 39.74%. The accumulated phenolics hardly helped detoxify ROS in vivo in absence of oxidative stress, but the elevated phenolic synthesis might restrict ROS generation by consuming reduction equivalents. As with NaCl stress, soil salinity also increased concentrations of leaf phenolics including chlorogenic acid and luteolosid without exacerbated lipid peroxidation. In conclusion, leaf phenolics accumulation is a mechanism for the acclimation to saline stress probably by preventing oxidative stress in honeysuckle; leaf medicinal quality of honeysuckle can be improved by saline stress due to the accumulation of bioactive phenolic compounds.
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contrasting photosynthesis and photoinhibition in tetraploid and its autodiploid honeysuckle Lonicera japonica thunb under salt stress
Frontiers in Plant Science, 2015Co-Authors: Congwen Wu, Lihua Zhang, Xiaobing ChenAbstract:Honeysuckle (Lonicera japonica Thunb.) is a popular landscape plant. This study was to explore leaf photosynthetic characterization with emphasis on the coordination between photosystem II (PSII) and photosystem I (PSI) in tetraploid and its autodiploid honeysuckle (TH and DH) upon salt stress (300 mM NaCl). Leaf photosynthetic rate and carboxylation efficiency in DH and TH were significantly decreased under salt stress, and the decrease was greater in DH. PSII photoinhibition was induced in DH under salt stress, as the maximum quantum yield of PSII (Fv/Fm) was significantly decreased. PSII photoinhibition declined electron flow to PSI, but did not prevent PSI photoinhibition, as the maximal photochemical capacity of PSI (Delta MR/MR0) was significantly decreased by salt stress. According to the significant decrease in PSI oxidation amplitude in the first 1 s red illumination, PSI photoinhibition was more severe than PSII photoinhibition. As a result, PSII and PSI coordination was destroyed. Comparatively, salt-induced photoinhibition did not occur in TH, as no significant change was observed in Fv/Fm and Delta MR/MR0. Consequently, PSII and PSI coordination was not significantly affected by salt stress. In conclusion, TH maintained normal coordination between PSII and PSI by preventing photoinhibition and exhibited higher leaf photosynthetic activity than DH under salt stress. Compared with DH, lower leaf ionic toxicity due to greater root Na+ extrusion and restriction of Na+ transport to leaf might be responsible for maintaining higher leaf photosynthetic capacity in TH under salt stress.
Zhouli Liu - One of the best experts on this subject based on the ideXlab platform.
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influence of cd2 on growth and chlorophyll fluorescence in a hyperaccumulator Lonicera japonica thunb
Journal of Plant Growth Regulation, 2015Co-Authors: Zhouli Liu, Wei ChenAbstract:Chlorophyll fluorescence is an important instrument to study the responses of plants to cadmium (Cd) stress, which in turn can provide a better understanding of Cd tolerance in plants. In the present study, the influence due to cadmium (Cd2+) exposure on growth and chlorophyll fluorescence was investigated in a new hyperaccumulator—Lonicera japonica Thunb. Four levels of Cd2+ (0, 5, 25, and 125 mg kg−1) were added to the soil. After 90 days of Cd2+ exposure, maximal photochemical efficiency of photosystem II (F v/F m) and effective quantum yield of photosystem II (ΦPS II) of L. japonica showed an increase under 5 mg kg−1 Cd2+ exposure, but decreased under higher concentrations of Cd2+exposure. However, when Cd concentrations in soil were up to the highest concentrations (125 mg kg−1), no significant differences of F v/F m, ΦPS II, photochemical quenching, chlorophyll, and carotenoid contents compared with the control were observed. These results indicate that the good tolerance of L. japonica to Cd might result from effective mechanisms including the capacity to maintain good growth, photosynthetic pigment composition, and chlorophyll fluorescence activity, which would be beneficial to enhance the potential for phytoremediation.
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hormesis effects induced by cadmium on growth and photosynthetic performance in a hyperaccumulator Lonicera japonica thunb
Journal of Plant Growth Regulation, 2015Co-Authors: Lian Jia, Zhouli Liu, Wei ChenAbstract:A soil experiment was designed to investigate the hormesis effect of cadmium (Cd) on the growth and the photosynthetic performance in a hyperaccumulator, Lonicera japonica Thunb. by measuring gas exchange, chlorophyll fluorescence parameters, and photosynthetic pigments. After 90 days of exposure to soil containing 25 mg kg−1 Cd, shoot Cd concentrations reached 168.27 ± 5.01 μg g−1 dry weight, without showing symptoms of visible damage to the plants. The results also show that Cd at low concentrations (≤10 mg kg−1) induced a significant increase in plant biomass, net photosynthetic rate (P n), content of chlorophyll (a, b, and a+b) and carotenoids, effective quantum yield ΦPSII and photochemical quenching coefficient q p, but inhibited them at high concentrations (>25 mg kg−1), confirming a hormetic response. The observed growth increases were closely related to the increase in net photosynthesis induced by Cd, though the causes of the P n increase are still not understood. The present study suggested that hormetic effects should be taken into consideration in phytoremediation of Cd-contaminated soil and the dose range of Cd inducing hormesis on L. japonica is proposed as 2.5–10 mg kg−1 in the soil.
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hormesis phenomena under cd stress in a hyperaccumulator Lonicera japonica thunb
Ecotoxicology, 2013Co-Authors: Lian Jia, Wei Chen, Zhouli Liu, Yanqing HuangAbstract:A hydroponic experiment was carried out to investigate possible hormetic response induced by cadmium (Cd) in a potential hyperaccumulator-Lonicera japonica Thunb. The results showed that Cd at low concentrations induced a significant increase in plant growth, leaf water content and content of photosynthetic pigments in L. japonica, but decreased them at high concentrations, displayed inverted U-shaped dose response curves, confirming a typical biphasic hormetic response. The U-shaped dose response curves were displayed in malondialdehyde (MDA) and electrolyte leakage in leaves at low doses of Cd, indicating reduce oxidative stress and toxic effect. The increase of superoxide dismutase (SOD) and catalase (CAT) activities was observed along with the increased Cd concentration, indicative of increase in anti-oxidative capacity that ensures redox homeostasis is maintained. After 28 days exposure to 10 mg L−1 Cd, stem and leaf Cd concentrations reached 502.96 ± 28.90 and 103.22 ± 5.62 mg kg−1 DW, respectively and the plant had high bioaccumulation coefficient (BC) and translocation factor (TF′). Moreover, the maximum TF value was found at 2.5 mg L−1 Cd treatment, implying that low Cd treatment improved the ability to transfer Cd from medium via roots to aerial structures. Taking together, L. japonica could be considered as a new plant to investigate the underlying mechanisms of hormesis and Cd tolerance. Our results suggest that hormetic effects should be taken into consideration in phytoremediation of Cd-contaminated soil.
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effects of cadmium hyperaccumulation on the concentrations of four trace elements in Lonicera japonica thunb
Ecotoxicology, 2011Co-Authors: Zhouli Liu, Wei ChenAbstract:Hyperaccumulators are important in the phytoremediation of cadmium (Cd)-contaminated soil. In this study, Cd accumulation and the interactions between Cd and four other trace elements (Fe, Mn, Cu, and Zn) in Lonicera japonica Thunb. were investigated. As a result of exposure to soil containing 50 mg kg−1 Cd, stem and shoot Cd concentrations reached 344.49 ± 0.71 and 286.12 ± 9.38 μg g−1 DW respectively, without showing symptoms of visible damage to the plants. This suggests that L. japonica has a strong tolerance to Cd. It is proposed that trace metal elements are involved in the Cd-detoxification mechanisms shown by hyperaccumulators. There is a synergistic interaction in accumulation and translocation between Cd and Fe and a significantly negative correlation between Cd and Cu or Zn concentrations in L. japonica plant tissues. The imbalanced trace element concentrations influences detoxification processes to Cd, therefore, L. japonica could be considered as a new Cd-hyperaccumulator model to investigate the metal tolerance strategies of plants.
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accumulation and tolerance characteristics of cadmium in a potential hyperaccumulator Lonicera japonica thunb
Journal of Hazardous Materials, 2009Co-Authors: Zhouli Liu, Wei Chen, Fenghui Yuan, Kun Yan, Dali TaoAbstract:Phytoremediation using hyperaccumulators is a promising technique of removing soil pollutants. In the study, growth responses, cadmium (Cd) accumulation capability and physiological mechanisms of Lonicera japonica Thunb. under Cd stress were investigated. Exposed to 5 and 10 mg L(-1) Cd, the plants did not show any visual symptoms, furthermore, the height, dry biomass of leaves, roots and total and the chlorophyll (CHL) content were obtained different grade increase. When the concentration of Cd was up to 50 mg L(-1), the height, dry biomass of leaves and roots had not significant differences compared with the control. The indexes of tolerance (IT) were all above 0.8. The maintenance of high superoxide dismutase (SOD) and catalase (CAT) activities was observed along with the increased Cd concentration, suggesting strong internal detoxification mechanisms inside plant cells. After 21 days exposure to 25 mg L(-1) Cd, stem and shoot Cd concentrations reached 344.49+/-0.71 and 286.12+/-9.38 microg g(-1) DW, respectively and the plant had higher bioaccumulation coefficient (BC) and translocation factor (TF). According to these results, it was shown L. japonica had strong tolerance and accumulation capability to Cd, therefore it is a potential Cd-hyperaccumulator.
Congwen Wu - One of the best experts on this subject based on the ideXlab platform.
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contrasting photosynthesis and photoinhibition in tetraploid and its autodiploid honeysuckle Lonicera japonica thunb under salt stress
Frontiers in Plant Science, 2015Co-Authors: Congwen Wu, Lihua Zhang, Xiaobing ChenAbstract:Honeysuckle (Lonicera japonica Thunb.) is a popular landscape plant. This study was to explore leaf photosynthetic characterization with emphasis on the coordination between photosystem II (PSII) and photosystem I (PSI) in tetraploid and its autodiploid honeysuckle (TH and DH) upon salt stress (300 mM NaCl). Leaf photosynthetic rate and carboxylation efficiency in DH and TH were significantly decreased under salt stress, and the decrease was greater in DH. PSII photoinhibition was induced in DH under salt stress, as the maximum quantum yield of PSII (Fv/Fm) was significantly decreased. PSII photoinhibition declined electron flow to PSI, but did not prevent PSI photoinhibition, as the maximal photochemical capacity of PSI (Delta MR/MR0) was significantly decreased by salt stress. According to the significant decrease in PSI oxidation amplitude in the first 1 s red illumination, PSI photoinhibition was more severe than PSII photoinhibition. As a result, PSII and PSI coordination was destroyed. Comparatively, salt-induced photoinhibition did not occur in TH, as no significant change was observed in Fv/Fm and Delta MR/MR0. Consequently, PSII and PSI coordination was not significantly affected by salt stress. In conclusion, TH maintained normal coordination between PSII and PSI by preventing photoinhibition and exhibited higher leaf photosynthetic activity than DH under salt stress. Compared with DH, lower leaf ionic toxicity due to greater root Na+ extrusion and restriction of Na+ transport to leaf might be responsible for maintaining higher leaf photosynthetic capacity in TH under salt stress.
Yan Jiang Qiao - One of the best experts on this subject based on the ideXlab platform.
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multivariate detection limits of on line nir model for extraction process of chlorogenic acid from Lonicera japonica
Journal of Pharmaceutical and Biomedical Analysis, 2013Co-Authors: Chenglin Sui, Xinyuan Shi, Yan Jiang QiaoAbstract:a b s t r a c t A methodology is proposed to estimate the multivariate detection limits (MDL) of on-line near-infrared (NIR) model in Chinese Herbal Medicines (CHM) system. In this paper, Lonicera japonica was used as an example, and its extraction process was monitored by on-line NIR spectroscopy. Spectra of on-line NIR could be collected by two fiber optic probes designed to transmit NIR radiation by a 2 mm-flange. High performance liquid chromatography (HPLC) was used as a reference method to determine the content of chlorogenic acid in the extract solution. Multivariate calibration models were carried out including partial least squares regression (PLS) and interval partial least-squares (iPLS). The result showed improvement of model performance: compared with PLS model, the root mean square errors of prediction (RMSEP) of iPLS model decreased from 0.111 mg to 0.068 mg, and the R2 parameter increased from 0.9434 to 0.9801. Furthermore, MDL values were determined by a multivariate method using the type of errors and concentration ranges. The MDL of iPLS model was about 14 ppm, which confirmed that on-line NIR spectroscopy had the ability to detect trace amounts of chlorogenic acid in L. japonica. As a result, the application of on-line NIR spectroscopy for monitoring extraction process in CHM could be very encouraging and reliable. © 2013 Published by Elsevier B.V.
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validation of a nir quantification method for the determination of chlorogenic acid in Lonicera japonica solution in ethanol precipitation process
Journal of Pharmaceutical and Biomedical Analysis, 2012Co-Authors: Zhisheng Wu, Bing Xu, Min Du, Yan Jiang QiaoAbstract:Abstract The feasibility of near-infrared spectroscopy (NIRS) for chlorogenic acid content analysis in ethanol precipitation process of water extract of Lonicera japonica was verified in this work. A calibration and validation set was designed for the conception and evaluation of the method adequacy. An experimental protocol was then followed, involving two different NIR instruments for data acquisition. On the basis of this protocol, the model was developed based on partial least squares regression (PLS) and the determination coefficient ( R 2 cal and R 2 val ), standard error of calibration and prediction (SEC and SEP) were 0.9962, 0.9955, 111.1 μg/mL and 107.1 μg/mL for Holographic Grating NIR instrument, and 0.9984, 0.9971, 53.6 μg/mL and 83.3 μg/mL for Fourier Transform NIR instrument. However, such above criteria did not clearly demonstrate the model's prediction error over each analyzed content range. Consequently, a novel approach based on accuracy profile which allowed the acquisition of the lower limit of quantification (LLOQ) was used to validate the robustness and accuracy of PLS model. The resulting accuracy profile showed that PLS model was able to determine chlorogenic acid content by two NIR systems, whose LLOQ was about 1550 μg/mL. It was concluded that the two NIR systems were suitable for use as Process Analytical Technology (PAT) to understand ethanol precipitation process of water extract of Lonicera japonica.
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insecticidal activity of the essential oil of Lonicera japonica flower buds and its main constituent compounds against two grain storage insects
Journal of Medicinal Plants Research, 2012Co-Authors: Hai Yan Zhou, Kai Yang, Na Na Zhao, Cheng Fang Wang, Yan Jiang QiaoAbstract:The aim of this research was to determine acute toxicity of the essential oil of Lonicera japonica Thunb. (Caprifoliaceae) flower buds against the booklouse (Liposcelis bostrychophila Badonnel) and the maize weevils (Sitophilus zeamais Motschulsky). Essential oil of L. japonica flower buds was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 25 components of the essential oil were identified. The principal compounds in the essential oil were estragole (80.17%) and linalool (6.05%). The essential oil exhibited strong contact toxicity against S. zeamais and L. bostrychophila with LD50 values of 21.54 µg/adult and 64.04 µg/cm2, respectively. The constituent compounds, estragole (LD50 = 49.95 µg/cm2) and linalool (LD50 = 172.54 µg/cm2) also possessed contact toxicity against L. bostrychophila. L. japonica essential oil and its constituent compounds (estragole and linalool) exhibited fumigant toxicity against S. zeamaiswith LC50 values of 13.36, 14.10 and 10.46 mg/L, respectively. The essential oil of L. japonica(LC50 = 0.20 mg/L) and its constituent compounds, estragole (LC50 = 0.16 mg/L) and linalool (LC50 = 0.41 mg/L) possessed fumigant toxicity against L. bostrychophila. The results indicated that the essential oil of L. japonica and its constituent compounds showed potential in terms of contact and fumigant toxicity against grain storage insects. Key words: Lonicera japonica, Liposcelis bostrychophila, Sitophilus zeamais, contact toxicity, fumigant, essential oil composition, estragole, linalool.
