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Kanji Ohyama - One of the best experts on this subject based on the ideXlab platform.
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Isolation and Characterization of Δ^6-Desaturase, an ELO-Like Enzyme and Δ^5-Desaturase from the Liverwort Marchantia Polymorpha and Production of Arachidonic and Eicosapentaenoic Acids in the Methylotrophic Yeast Pichia Pastoris
Plant Molecular Biology, 2004Co-Authors: Masataka Kajikawa, Katsuyuki T. Yamato, Yoshito Kohzu, Masutoshi Nojiri, Eiji Sakuradani, Sakayu Shimizu, Yasuyoshi Sakai, Hideya Fukuzawa, Kanji OhyamaAbstract:The liverwort Marchantia polymorpha contains high proportions of arachidonic and eicosapentaenoic acids. In general, these C20 polyunsaturated fatty acids (PUFA) are synthesized from linoleic and α -linolenic acids, respectively, by a series of reactions catalyzed by Δ^6-desaturase, an ELO-like enzyme involved in Δ^6 elongation and Δ^5-desaturase. Here we report the isolation and characterization of the cDNAs, MpDES6 , MpELO1 and MpDES5 , coding for the respective enzymes from M. polymorpha. Co-expression of the MpDES6 , MpELO1 and MpDES5 cDNAs resulted in the accumulation of arachidonic and eicosapentaenoic acids in the methylotrophic yeast Pichia pastoris . Interestingly, Δ^6 desaturation by the expression of the MpDES6 cDNA appears to occur both in glycerolipids and the acyl-CoA pool, although other Lower-Plant Δ^6-desaturases are known to have a strong preference for glycerolipids.
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isolation and characterization of delta 6 desaturase an elo like enzyme and delta 5 desaturase from the liverwort marchantia polymorpha and production of arachidonic and eicosapentaenoic acids in the methylotrophic yeast pichia pastoris
Plant Molecular Biology, 2004Co-Authors: Masataka Kajikawa, Katsuyuki T. Yamato, Yoshito Kohzu, Masutoshi Nojiri, Eiji Sakuradani, Sakayu Shimizu, Yasuyoshi Sakai, Hideya Fukuzawa, Kanji OhyamaAbstract:The liverwort Marchantia polymorpha contains high proportions of arachidonic and eicosapentaenoic acids. In general, these C20 polyunsaturated fatty acids (PUFA) are synthesized from linoleic and alpha -linolenic acids, respectively, by a series of reactions catalyzed by Delta(6)-desaturase, an ELO-like enzyme involved in Delta(6) elongation and Delta(5)-desaturase. Here we report the isolation and characterization of the cDNAs, MpDES6, MpELO1 and MpDES5, coding for the respective enzymes from M. polymorpha. Co-expression of the MpDES6, MpELO1 and MpDES5 cDNAs resulted in the accumulation of arachidonic and eicosapentaenoic acids in the methylotrophic yeast Pichia pastoris. Interestingly, Delta(6) desaturation by the expression of the MpDES6 cDNA appears to occur both in glycerolipids and the acyl-CoA pool, although other Lower-Plant Delta(6)-desaturases are known to have a strong preference for glycerolipids.
Dibyendu Adhikari - One of the best experts on this subject based on the ideXlab platform.
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Microenvironmental heterogeneity caused by anthropogenic LULC foster Lower Plant assemblages in the riparian habitats of lentic systems in tropical floodplains.
The Science of the total environment, 2018Co-Authors: Poppy Rajbongshi, Tapati Das, Dibyendu AdhikariAbstract:Abstract Anthropogenic land use and land cover (LULC) create a heterogeneous environment in the floodplains. This heterogeneity may be governing Plant species assemblages, diversity, and dominance patterns in the riparian habitats of the lentic systems in tropical floodplains. We tested this hypothesis in the floodplains of Barak river basin in northeast India following standard methods of Plant and soil sampling/analysis and multivariate statistical tools. Plant community studies in the riparian habitats of the selected lentic systems were done at monthly intervals for a period of one year, while soil sampling and analysis were done at bimonthly intervals. Standard data visualization plots and canonical correspondence analysis (CCA) were used to assess spatiotemporal variations in species richness and diversity, environmental heterogeneity, and species-environment association. The study revealed that anthropogenic land use and land cover significantly affects species assemblage, diversity, and dominance in the riparian habitats. The variations in vegetation structure and composition with respect to the adjoining land use type plausibly have implications on the structure and functioning of the lentic systems. Thus, the study recommends that a holistic approach involving the riparian areas is required for effective management of tropical floodplains.
Masataka Kajikawa - One of the best experts on this subject based on the ideXlab platform.
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Isolation and Characterization of Δ^6-Desaturase, an ELO-Like Enzyme and Δ^5-Desaturase from the Liverwort Marchantia Polymorpha and Production of Arachidonic and Eicosapentaenoic Acids in the Methylotrophic Yeast Pichia Pastoris
Plant Molecular Biology, 2004Co-Authors: Masataka Kajikawa, Katsuyuki T. Yamato, Yoshito Kohzu, Masutoshi Nojiri, Eiji Sakuradani, Sakayu Shimizu, Yasuyoshi Sakai, Hideya Fukuzawa, Kanji OhyamaAbstract:The liverwort Marchantia polymorpha contains high proportions of arachidonic and eicosapentaenoic acids. In general, these C20 polyunsaturated fatty acids (PUFA) are synthesized from linoleic and α -linolenic acids, respectively, by a series of reactions catalyzed by Δ^6-desaturase, an ELO-like enzyme involved in Δ^6 elongation and Δ^5-desaturase. Here we report the isolation and characterization of the cDNAs, MpDES6 , MpELO1 and MpDES5 , coding for the respective enzymes from M. polymorpha. Co-expression of the MpDES6 , MpELO1 and MpDES5 cDNAs resulted in the accumulation of arachidonic and eicosapentaenoic acids in the methylotrophic yeast Pichia pastoris . Interestingly, Δ^6 desaturation by the expression of the MpDES6 cDNA appears to occur both in glycerolipids and the acyl-CoA pool, although other Lower-Plant Δ^6-desaturases are known to have a strong preference for glycerolipids.
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isolation and characterization of delta 6 desaturase an elo like enzyme and delta 5 desaturase from the liverwort marchantia polymorpha and production of arachidonic and eicosapentaenoic acids in the methylotrophic yeast pichia pastoris
Plant Molecular Biology, 2004Co-Authors: Masataka Kajikawa, Katsuyuki T. Yamato, Yoshito Kohzu, Masutoshi Nojiri, Eiji Sakuradani, Sakayu Shimizu, Yasuyoshi Sakai, Hideya Fukuzawa, Kanji OhyamaAbstract:The liverwort Marchantia polymorpha contains high proportions of arachidonic and eicosapentaenoic acids. In general, these C20 polyunsaturated fatty acids (PUFA) are synthesized from linoleic and alpha -linolenic acids, respectively, by a series of reactions catalyzed by Delta(6)-desaturase, an ELO-like enzyme involved in Delta(6) elongation and Delta(5)-desaturase. Here we report the isolation and characterization of the cDNAs, MpDES6, MpELO1 and MpDES5, coding for the respective enzymes from M. polymorpha. Co-expression of the MpDES6, MpELO1 and MpDES5 cDNAs resulted in the accumulation of arachidonic and eicosapentaenoic acids in the methylotrophic yeast Pichia pastoris. Interestingly, Delta(6) desaturation by the expression of the MpDES6 cDNA appears to occur both in glycerolipids and the acyl-CoA pool, although other Lower-Plant Delta(6)-desaturases are known to have a strong preference for glycerolipids.
Ray Wu - One of the best experts on this subject based on the ideXlab platform.
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wheat lea genes pma80 and pma1959 enhance dehydration tolerance of transgenic rice oryza sativa l
Molecular Breeding, 2002Co-Authors: Zaiquan Cheng, Jayaprakash Targolli, Xingqi Huang, Ray WuAbstract:Drought and salt stresses are two major factors that Lower Plant productivity. Transgenic approaches offer powerful means to better understand and then minimize loss of yield due to these abiotic stresses. In this study, we have generated transgenic rice Plants expressing a wheat LEA group 2 protein (PMA80) gene, and separately the wheat LEA group 1 protein (PMA1959) gene. Molecular analysis of the transgenic Plants revealed the stable integration of the transgenes. Immunoblot analysis showed the presence of the LEA group 2 protein (39 kDa) and the LEA group 1 protein (25 kDa) in most of the Plant lines. Second-generation transgenic Plants were subjected to dehydration or salt stress. The results showed that accumulation of either PMA80 or PMA1959 correlates with increased tolerance of transgenic rice Plants to these stresses.
Poppy Rajbongshi - One of the best experts on this subject based on the ideXlab platform.
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Microenvironmental heterogeneity caused by anthropogenic LULC foster Lower Plant assemblages in the riparian habitats of lentic systems in tropical floodplains.
The Science of the total environment, 2018Co-Authors: Poppy Rajbongshi, Tapati Das, Dibyendu AdhikariAbstract:Abstract Anthropogenic land use and land cover (LULC) create a heterogeneous environment in the floodplains. This heterogeneity may be governing Plant species assemblages, diversity, and dominance patterns in the riparian habitats of the lentic systems in tropical floodplains. We tested this hypothesis in the floodplains of Barak river basin in northeast India following standard methods of Plant and soil sampling/analysis and multivariate statistical tools. Plant community studies in the riparian habitats of the selected lentic systems were done at monthly intervals for a period of one year, while soil sampling and analysis were done at bimonthly intervals. Standard data visualization plots and canonical correspondence analysis (CCA) were used to assess spatiotemporal variations in species richness and diversity, environmental heterogeneity, and species-environment association. The study revealed that anthropogenic land use and land cover significantly affects species assemblage, diversity, and dominance in the riparian habitats. The variations in vegetation structure and composition with respect to the adjoining land use type plausibly have implications on the structure and functioning of the lentic systems. Thus, the study recommends that a holistic approach involving the riparian areas is required for effective management of tropical floodplains.