The Experts below are selected from a list of 29904 Experts worldwide ranked by ideXlab platform
Klemens Rappersberger - One of the best experts on this subject based on the ideXlab platform.
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immunoelectron microscopic characterization of human dermal lymphatic microvascular endothelial Cells differential expression of cd31 cd34 and type iv collagen with lymphatic endothelial Cells vs blood capillary endothelial Cells in normal human skin
Journal of Histochemistry and Cytochemistry, 1998Co-Authors: Birthe Sauter, Dagmar Foedinger, Klaus Wolff, Barbara Sterniczky, Klemens RappersbergerAbstract:We performed a comparative investigation of the immunomorphological characteristics of lymphatic and blood microvascular endothelial Cells in normal human skin, cutaneous lymphangiomas, and hemangiomas, employing a pre-embedding immunogold electron microscopic technique. We stained for Cell membrane proteins that are commonly used for light microscopic characterization of blood endothelial Cells. With blood microvascular endothelial Cells, we observed uniform labeling of the Luminal Cell membranes with monoclonal antibodies (MAbs) JC70 (CD31), EN-4 (CD31), BMA120, PAL-E, and QBEND-10 (CD34), and strong staining of the vascular basal lamina for Type IV collagen under normal and pathological conditions. In contrast, lymphatic microvascular endothelial Cells in normal human skin and in lymphangiomas displayed, in addition to a Luminal labeling, pronounced expression of CD31 and CD34 along the abLuminal Cell membranes. Moreover, CD31 was preferentially detected within interCellular junctions. The expression o...
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immunoelectron microscopic characterization of human dermal lymphatic microvascular endothelial Cells differential expression of cd31 cd34 and type iv collagen with lymphatic endothelial Cells vs blood capillary endothelial Cells in normal human skin lymphangioma and hemangioma in situ
Journal of Histochemistry and Cytochemistry, 1998Co-Authors: Birthe Sauter, Dagmar Foedinger, Klaus Wolff, Barbara Sterniczky, Klemens RappersbergerAbstract:We performed a comparative investigation of the immunomorphological characteristics of lymphatic and blood microvascular endothelial Cells in normal human skin, cutaneous lymphangiomas, and hemangiomas, employing a pre-embedding immunogold electron microscopic technique. We stained for Cell membrane proteins that are commonly used for light microscopic characterization of blood endothelial Cells. With blood microvascular endothelial Cells, we observed uniform labeling of the Luminal Cell membranes with monoclonal antibodies (MAbs) JC70 (CD31), EN-4 (CD31), BMA120, PAL-E, and QBEND-10 (CD34), and strong staining of the vascular basal lamina for Type IV collagen under normal and pathological conditions. In contrast, lymphatic microvascular endothelial Cells in normal human skin and in lymphangiomas displayed, in addition to a Luminal labeling, pronounced expression of CD31 and CD34 along the abLuminal Cell membranes. Moreover, CD31 was preferentially detected within interCellular junctions. The expression of CD34 was mostly confined to abLuminal endothelial microprocesses and was upregulated in lymphangiomas and hemangiomas. Type IV collagen partially formed the Luminal lining of initial lymphatics and occasionally formed bridges over interendothelial gaps. Our findings suggest a function of transmigration protein CD31 in recruitment of dendritic Cells into the lymphatic vasculature. CD34 labeling may indicate early endothelial Cell sprouting. The distribution of Type IV collagen also supports its role as a signal for migration and tube formation for lymphatic endothelial Cells.
Birthe Sauter - One of the best experts on this subject based on the ideXlab platform.
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immunoelectron microscopic characterization of human dermal lymphatic microvascular endothelial Cells differential expression of cd31 cd34 and type iv collagen with lymphatic endothelial Cells vs blood capillary endothelial Cells in normal human skin
Journal of Histochemistry and Cytochemistry, 1998Co-Authors: Birthe Sauter, Dagmar Foedinger, Klaus Wolff, Barbara Sterniczky, Klemens RappersbergerAbstract:We performed a comparative investigation of the immunomorphological characteristics of lymphatic and blood microvascular endothelial Cells in normal human skin, cutaneous lymphangiomas, and hemangiomas, employing a pre-embedding immunogold electron microscopic technique. We stained for Cell membrane proteins that are commonly used for light microscopic characterization of blood endothelial Cells. With blood microvascular endothelial Cells, we observed uniform labeling of the Luminal Cell membranes with monoclonal antibodies (MAbs) JC70 (CD31), EN-4 (CD31), BMA120, PAL-E, and QBEND-10 (CD34), and strong staining of the vascular basal lamina for Type IV collagen under normal and pathological conditions. In contrast, lymphatic microvascular endothelial Cells in normal human skin and in lymphangiomas displayed, in addition to a Luminal labeling, pronounced expression of CD31 and CD34 along the abLuminal Cell membranes. Moreover, CD31 was preferentially detected within interCellular junctions. The expression o...
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immunoelectron microscopic characterization of human dermal lymphatic microvascular endothelial Cells differential expression of cd31 cd34 and type iv collagen with lymphatic endothelial Cells vs blood capillary endothelial Cells in normal human skin lymphangioma and hemangioma in situ
Journal of Histochemistry and Cytochemistry, 1998Co-Authors: Birthe Sauter, Dagmar Foedinger, Klaus Wolff, Barbara Sterniczky, Klemens RappersbergerAbstract:We performed a comparative investigation of the immunomorphological characteristics of lymphatic and blood microvascular endothelial Cells in normal human skin, cutaneous lymphangiomas, and hemangiomas, employing a pre-embedding immunogold electron microscopic technique. We stained for Cell membrane proteins that are commonly used for light microscopic characterization of blood endothelial Cells. With blood microvascular endothelial Cells, we observed uniform labeling of the Luminal Cell membranes with monoclonal antibodies (MAbs) JC70 (CD31), EN-4 (CD31), BMA120, PAL-E, and QBEND-10 (CD34), and strong staining of the vascular basal lamina for Type IV collagen under normal and pathological conditions. In contrast, lymphatic microvascular endothelial Cells in normal human skin and in lymphangiomas displayed, in addition to a Luminal labeling, pronounced expression of CD31 and CD34 along the abLuminal Cell membranes. Moreover, CD31 was preferentially detected within interCellular junctions. The expression of CD34 was mostly confined to abLuminal endothelial microprocesses and was upregulated in lymphangiomas and hemangiomas. Type IV collagen partially formed the Luminal lining of initial lymphatics and occasionally formed bridges over interendothelial gaps. Our findings suggest a function of transmigration protein CD31 in recruitment of dendritic Cells into the lymphatic vasculature. CD34 labeling may indicate early endothelial Cell sprouting. The distribution of Type IV collagen also supports its role as a signal for migration and tube formation for lymphatic endothelial Cells.
Mark Shackleton - One of the best experts on this subject based on the ideXlab platform.
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gata 3 is an essential regulator of mammary gland morphogenesis and Luminal Cell differentiation
Nature Cell Biology, 2007Co-Authors: Marie Liesse Asselinlabat, Mark Shackleton, Kate D Sutherland, Holly E Barker, Richard Thomas, Natasha C ForrestAbstract:The transcription factor Gata-3 is a defining marker of the 'Luminal' subtypes of breast cancer. To gain insight into the role of Gata-3 in breast epithelial development and oncogenesis, we have explored its normal function within the mammary gland by conditionally deleting Gata-3 at different stages of development. We report that Gata-3 has essential roles in the morphogenesis of the mammary gland in both the embryo and adult. Through the discovery of a novel marker (beta3-integrin) of Luminal progenitor Cells and their purification, we demonstrate that Gata-3 deficiency leads to an expansion of Luminal progenitors and a concomitant block in differentiation. Remarkably, introduction of Gata-3 into a stem Cell-enriched population induced maturation along the alveolar Luminal lineage. These studies provide evidence for the existence of an epithelial hierarchy within the mammary gland and establish Gata-3 as a critical regulator of Luminal differentiation.
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gata 3 is an essential regulator of mammary gland morphogenesis and Luminal Cell differentiation
Nature Cell Biology, 2007Co-Authors: Marie Liesse Asselinlabat, Mark Shackleton, Kate D Sutherland, Holly E Barker, Richard Thomas, Natasha C ForrestAbstract:Gata-3 is an essential regulator of mammary-gland morphogenesis and Luminal-Cell differentiation
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steroid hormone receptor status of mouse mammary stem Cells
Journal of the National Cancer Institute, 2006Co-Authors: Marie Liesse Asselinlabat, John Stingl, Mark Shackleton, Francois Vaillant, Natasha C Forrest, Connie J Eaves, Jane E Visvader, Geoffrey J LindemanAbstract:The estrogen receptor alpha (ERalpha), progesterone receptor (PR), and erbB2 (Her2 in humans) are important prognostic markers of human breast cancer, and they are variably expressed in different subtypes of breast cancer. The basal subtype, for example, is negative for ERalpha, PR, and Her2 by immunohistochemistry. We investigated the expression of these signaling molecules in enriched populations of mouse mammary stem Cells and Luminal Cells that were isolated according to their differential expression of CD24 and the alpha6beta1-integrin complex. We found that the basal population, which is enriched in mouse mammary stem Cells, did not express ERalpha, PR, or ErbB2/Her2 but did express epidermal growth factor receptor (EGFR)/ErbB1, whereas the subset of Cells enriched for Luminal Cells expressed ERalpha (37% of Cells) and PR (40% of Cells) but not ErbB2/Her2 or EGFR/ErbB1. Ovariectomy confirmed the importance of estrogen signaling to Luminal Cell proliferation but had no effect on the size of the mouse mammary stem Cell-enriched population. Thus, mouse mammary stem Cells were negative for ERalpha, PR, and ErbB2 and appeared to share common properties with poor-prognosis basal breast cancer.
Klaus Wolff - One of the best experts on this subject based on the ideXlab platform.
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immunoelectron microscopic characterization of human dermal lymphatic microvascular endothelial Cells differential expression of cd31 cd34 and type iv collagen with lymphatic endothelial Cells vs blood capillary endothelial Cells in normal human skin
Journal of Histochemistry and Cytochemistry, 1998Co-Authors: Birthe Sauter, Dagmar Foedinger, Klaus Wolff, Barbara Sterniczky, Klemens RappersbergerAbstract:We performed a comparative investigation of the immunomorphological characteristics of lymphatic and blood microvascular endothelial Cells in normal human skin, cutaneous lymphangiomas, and hemangiomas, employing a pre-embedding immunogold electron microscopic technique. We stained for Cell membrane proteins that are commonly used for light microscopic characterization of blood endothelial Cells. With blood microvascular endothelial Cells, we observed uniform labeling of the Luminal Cell membranes with monoclonal antibodies (MAbs) JC70 (CD31), EN-4 (CD31), BMA120, PAL-E, and QBEND-10 (CD34), and strong staining of the vascular basal lamina for Type IV collagen under normal and pathological conditions. In contrast, lymphatic microvascular endothelial Cells in normal human skin and in lymphangiomas displayed, in addition to a Luminal labeling, pronounced expression of CD31 and CD34 along the abLuminal Cell membranes. Moreover, CD31 was preferentially detected within interCellular junctions. The expression o...
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immunoelectron microscopic characterization of human dermal lymphatic microvascular endothelial Cells differential expression of cd31 cd34 and type iv collagen with lymphatic endothelial Cells vs blood capillary endothelial Cells in normal human skin lymphangioma and hemangioma in situ
Journal of Histochemistry and Cytochemistry, 1998Co-Authors: Birthe Sauter, Dagmar Foedinger, Klaus Wolff, Barbara Sterniczky, Klemens RappersbergerAbstract:We performed a comparative investigation of the immunomorphological characteristics of lymphatic and blood microvascular endothelial Cells in normal human skin, cutaneous lymphangiomas, and hemangiomas, employing a pre-embedding immunogold electron microscopic technique. We stained for Cell membrane proteins that are commonly used for light microscopic characterization of blood endothelial Cells. With blood microvascular endothelial Cells, we observed uniform labeling of the Luminal Cell membranes with monoclonal antibodies (MAbs) JC70 (CD31), EN-4 (CD31), BMA120, PAL-E, and QBEND-10 (CD34), and strong staining of the vascular basal lamina for Type IV collagen under normal and pathological conditions. In contrast, lymphatic microvascular endothelial Cells in normal human skin and in lymphangiomas displayed, in addition to a Luminal labeling, pronounced expression of CD31 and CD34 along the abLuminal Cell membranes. Moreover, CD31 was preferentially detected within interCellular junctions. The expression of CD34 was mostly confined to abLuminal endothelial microprocesses and was upregulated in lymphangiomas and hemangiomas. Type IV collagen partially formed the Luminal lining of initial lymphatics and occasionally formed bridges over interendothelial gaps. Our findings suggest a function of transmigration protein CD31 in recruitment of dendritic Cells into the lymphatic vasculature. CD34 labeling may indicate early endothelial Cell sprouting. The distribution of Type IV collagen also supports its role as a signal for migration and tube formation for lymphatic endothelial Cells.
Barbara Sterniczky - One of the best experts on this subject based on the ideXlab platform.
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immunoelectron microscopic characterization of human dermal lymphatic microvascular endothelial Cells differential expression of cd31 cd34 and type iv collagen with lymphatic endothelial Cells vs blood capillary endothelial Cells in normal human skin
Journal of Histochemistry and Cytochemistry, 1998Co-Authors: Birthe Sauter, Dagmar Foedinger, Klaus Wolff, Barbara Sterniczky, Klemens RappersbergerAbstract:We performed a comparative investigation of the immunomorphological characteristics of lymphatic and blood microvascular endothelial Cells in normal human skin, cutaneous lymphangiomas, and hemangiomas, employing a pre-embedding immunogold electron microscopic technique. We stained for Cell membrane proteins that are commonly used for light microscopic characterization of blood endothelial Cells. With blood microvascular endothelial Cells, we observed uniform labeling of the Luminal Cell membranes with monoclonal antibodies (MAbs) JC70 (CD31), EN-4 (CD31), BMA120, PAL-E, and QBEND-10 (CD34), and strong staining of the vascular basal lamina for Type IV collagen under normal and pathological conditions. In contrast, lymphatic microvascular endothelial Cells in normal human skin and in lymphangiomas displayed, in addition to a Luminal labeling, pronounced expression of CD31 and CD34 along the abLuminal Cell membranes. Moreover, CD31 was preferentially detected within interCellular junctions. The expression o...
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immunoelectron microscopic characterization of human dermal lymphatic microvascular endothelial Cells differential expression of cd31 cd34 and type iv collagen with lymphatic endothelial Cells vs blood capillary endothelial Cells in normal human skin lymphangioma and hemangioma in situ
Journal of Histochemistry and Cytochemistry, 1998Co-Authors: Birthe Sauter, Dagmar Foedinger, Klaus Wolff, Barbara Sterniczky, Klemens RappersbergerAbstract:We performed a comparative investigation of the immunomorphological characteristics of lymphatic and blood microvascular endothelial Cells in normal human skin, cutaneous lymphangiomas, and hemangiomas, employing a pre-embedding immunogold electron microscopic technique. We stained for Cell membrane proteins that are commonly used for light microscopic characterization of blood endothelial Cells. With blood microvascular endothelial Cells, we observed uniform labeling of the Luminal Cell membranes with monoclonal antibodies (MAbs) JC70 (CD31), EN-4 (CD31), BMA120, PAL-E, and QBEND-10 (CD34), and strong staining of the vascular basal lamina for Type IV collagen under normal and pathological conditions. In contrast, lymphatic microvascular endothelial Cells in normal human skin and in lymphangiomas displayed, in addition to a Luminal labeling, pronounced expression of CD31 and CD34 along the abLuminal Cell membranes. Moreover, CD31 was preferentially detected within interCellular junctions. The expression of CD34 was mostly confined to abLuminal endothelial microprocesses and was upregulated in lymphangiomas and hemangiomas. Type IV collagen partially formed the Luminal lining of initial lymphatics and occasionally formed bridges over interendothelial gaps. Our findings suggest a function of transmigration protein CD31 in recruitment of dendritic Cells into the lymphatic vasculature. CD34 labeling may indicate early endothelial Cell sprouting. The distribution of Type IV collagen also supports its role as a signal for migration and tube formation for lymphatic endothelial Cells.
