The Experts below are selected from a list of 1830 Experts worldwide ranked by ideXlab platform
M C Gomezguillen - One of the best experts on this subject based on the ideXlab platform.
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effect of functional edible films and high pressure processing on microbial and oxidative spoilage in cold smoked sardine sardina pilchardus
Food Chemistry, 2007Co-Authors: Joaquin Gomezestaca, P Montero, B Gimenez, M C GomezguillenAbstract:Abstract The present experiment was an attempt to improve the shelf-life of cold-smoked sardine (Sardina pilchardus) using, singly or in combination, high pressure (300 MPa/20 °C/15 min) and gelatin-based functional edible films enriched by adding an extract of oregano (Origanum vulgare) or rosemary (Rosmarinus officinalis) or by adding chitosan. The uncoated muscle itself exhibited a certain level of antioxidant power (as measured by the FRAP method) ensuing from the deposition of phenols during smoking. Coating the muscle with the films enriched with the oregano or rosemary extracts increased the phenol content and the antioxidant power of the muscle, particularly when used in association with high pressure, due to migration of antioxidant substances from the film. The edible films with the added plant extracts lowered lipid oxidation levels (as measured by the peroxide and TBARS indices) and also, to a lesser extent, reduced microbial growth (total counts), whereas the gelatin–chitosan-based edible films lowered microbial counts (total counts, sulphide-reducing Bacteria). Neither Luminescent Bacteria nor EnteroBacteriaceae were detected in any of the batches. The combination of high pressure and edible films yielded the best results in terms of both preventing oxidation and inhibiting microbial growth.
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a 4 hexylresorcinol based formulation to prevent melanosis and microbial growth in chilled tiger prawns marsupenaeus japonicus from aquaculture
Journal of Food Science, 2006Co-Authors: Oscar Martinezalvarez, P Montero, M E Lopezcaballero, M C GomezguillenAbstract:A study was made of the effect on melanosis, biochemical indexes, and microbial growth in tiger prawns (Marsupenaeus japonicus) from aquaculture, using a formulation containing 4-hexylresorcinol (0.1% and 0.05%) in combination with organic acids (citric, ascorbic, and acetic) and chelating agents (ethylenediaminetetraacetic acid [EDTA] and disodium dihydrogen pyrophosphate [PPi]). In vivo and postmortem application of treatment was evaluated. Prawns with no additives or treated with 4% of a commercial formula based on sulfites were used to compare with 4-hexylresorcinol. The formulations based on 4-hexylresorcinol or sulfites inhibited the polyphenoloxidase (PPO) activity under 0.2 (Δoptical density [OD]/min/mL), instead of 1 (ΔOD/min/mL) achieved by prawns without additives, with the consequent delay in the appearance of melanosis during the 1st wk of storage. Prawns treated with sulfites showed initially better protection; however 4-hexylresorcinol proved to be more effective at the end of storage. The formulation based on 4-hexylresorcinol at 0.1% concentration, provided in vivo, inhibited the microbial growth (total Bacteria count, H2S-producer microorganisms, lactic acid Bacteria, enteroBacteria, and pseudomonads), whereas the commercial sulfites inhibited the Luminescent Bacteria growth.
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role of sulfites and 4 hexylresorcinol in microbial growth and melanosis prevention of deepwater pink shrimp parapenaeus longirostris using a controlled atmosphere
Journal of Food Protection, 2005Co-Authors: Oscar Martinezalvarez, M C Gomezguillen, P MonteroAbstract:A controlled atmosphere containing 48% CO2 and 7% O2 was used in association with refrigeration for storage of deepwater pink shrimp (Parapenaeus longirostris). Shrimp were treated with two different concentrations of sodium metabisulfite or 4-hexylresorcinol and subjected to the controlled atmosphere immediately after capture onboard ship or on arrival in port. Total volatile basic nitrogen, total viable counts, enteroBacteria, lactic acid Bacteria, and Luminescent Bacteria were determined, and black spot progression was evaluated. The combined effect of controlled atmosphere and melanosis inhibitors was used to delay black spot development as compared to the shrimp stored in ice alone. Storage under the controlled atmosphere without ice limited microbiological quality, namely, total viable counts, but enteroBacterial growth was lower.
Isabel Villaescusa - One of the best experts on this subject based on the ideXlab platform.
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study on the toxicity of binary equitoxic mixtures of metals using the Luminescent Bacteria vibrio fischeri as a biological target
Chemosphere, 2005Co-Authors: E Fulladosa, Jeanclaude Murat, Isabel VillaescusaAbstract:Results from two mathematical approaches to predict the toxicity of all the possible binary equitoxic mixtures of Co, Cd, Cu, Zn and Pb were compared to the observed toxicity of these mixtures to Vibrio fischeri Bacteria. Combined effect of the metals was found to be antagonistic for Co-Cd, Cd-Zn, Cd-Pb and Cu-Pb, synergistic for Co-Cu and Zn-Pb and merely additive in other cases, revealing a complex pattern of possible interactions. Besides, Cd appears much less toxic to the Bacterial model than to animal cells. The synergistic effect of the Co-Cu combination and the strong lowering of Pb toxicity in the presence of Cd deserve much attention when establishing environmental safety regulations.
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effect of ph on arsenate and arsenite toxicity to Luminescent Bacteria vibrio fischeri
Archives of Environmental Contamination and Toxicology, 2004Co-Authors: E Fulladosa, Jeanclaude Murat, Maria Del Rosario Martinez Martinez, Isabel VillaescusaAbstract:Arsenic is an abundant metalloid and a dangerous pollutant when in solution under the arsenate or arsenite forms—As(V) and As(III), respectively. Since its biological effects are expected to depend on the oxidation state and on speciation, effect of pH on either As(V) or As(III) speciation and resulting toxicity was investigated using the Microtox bioassay based on change in light emission by the Luminescent Bacteria Vibrio fischeri. Within a 5.0–8.0 pH range, EC50 values for As(V) were found to decrease as pH became basic, reflecting an increase in toxicity; whereas in the case of As(III), EC50 values were almost unchanged within a 6.0–8.0 pH range and lowered only at pH 9.0. HAsO42− and H2AsO3− were found to be the most toxic species. A statistical approach based on testing the null hypothesis of additive toxicity revealed an antagonistic effect between the arsenate chemical species. At low concentrations, As(V) was regularly found to be more toxic than As(III), independent of the pH value. Conversely, at high concentrations, the toxicity of both As(III) and As(V) was found to chiefly depend on pH, as a consequence of the strong influence of this parameter on the chemical speciation.
Grzegorz Wȩgrzyn - One of the best experts on this subject based on the ideXlab platform.
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bioluminescence mediated stimulation of photoreactivation in Bacteria
Fems Microbiology Letters, 2005Co-Authors: Jowita Kozakiewicz, Magdalena Gajewska, Robert łyźen, Agata Czyź, Grzegorz WȩgrzynAbstract:Although biochemistry and genetics of light emission by cells have been investigated in detail, a biological role for Bacterial luminescence has remained obscure for a long time. It was proposed recently that luminescence may stimulate DNA repair, but the specific mechanism of this phenomenon was not investigated. Moreover, experiments showing decreased survival of UV-irradiated lux mutants relative to Luminescent cells were performed previously using only one Bacterial species, Vibrio harveyi. Here, we demonstrate that dark mutants of various strains of naturally Luminescent Bacteria (Photobacterium leiognathi, Photobacterium phosphoreum and Vibrio fischeri) are more sensitive to UV irradiation than wild-type cells. Thus, this phenomenon occurs not only in V. harveyi but also in other Bacterial species. Using an artificial system of Luminescent Escherichia coli in combination with phr mutants (defective in photolyase functions), we found that Bacterial luminescence may stimulate photoreactivation, perhaps by providing photons that are necessary for photolyase activity.
Oscar Martinezalvarez - One of the best experts on this subject based on the ideXlab platform.
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a 4 hexylresorcinol based formulation to prevent melanosis and microbial growth in chilled tiger prawns marsupenaeus japonicus from aquaculture
Journal of Food Science, 2006Co-Authors: Oscar Martinezalvarez, P Montero, M E Lopezcaballero, M C GomezguillenAbstract:A study was made of the effect on melanosis, biochemical indexes, and microbial growth in tiger prawns (Marsupenaeus japonicus) from aquaculture, using a formulation containing 4-hexylresorcinol (0.1% and 0.05%) in combination with organic acids (citric, ascorbic, and acetic) and chelating agents (ethylenediaminetetraacetic acid [EDTA] and disodium dihydrogen pyrophosphate [PPi]). In vivo and postmortem application of treatment was evaluated. Prawns with no additives or treated with 4% of a commercial formula based on sulfites were used to compare with 4-hexylresorcinol. The formulations based on 4-hexylresorcinol or sulfites inhibited the polyphenoloxidase (PPO) activity under 0.2 (Δoptical density [OD]/min/mL), instead of 1 (ΔOD/min/mL) achieved by prawns without additives, with the consequent delay in the appearance of melanosis during the 1st wk of storage. Prawns treated with sulfites showed initially better protection; however 4-hexylresorcinol proved to be more effective at the end of storage. The formulation based on 4-hexylresorcinol at 0.1% concentration, provided in vivo, inhibited the microbial growth (total Bacteria count, H2S-producer microorganisms, lactic acid Bacteria, enteroBacteria, and pseudomonads), whereas the commercial sulfites inhibited the Luminescent Bacteria growth.
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role of sulfites and 4 hexylresorcinol in microbial growth and melanosis prevention of deepwater pink shrimp parapenaeus longirostris using a controlled atmosphere
Journal of Food Protection, 2005Co-Authors: Oscar Martinezalvarez, M C Gomezguillen, P MonteroAbstract:A controlled atmosphere containing 48% CO2 and 7% O2 was used in association with refrigeration for storage of deepwater pink shrimp (Parapenaeus longirostris). Shrimp were treated with two different concentrations of sodium metabisulfite or 4-hexylresorcinol and subjected to the controlled atmosphere immediately after capture onboard ship or on arrival in port. Total volatile basic nitrogen, total viable counts, enteroBacteria, lactic acid Bacteria, and Luminescent Bacteria were determined, and black spot progression was evaluated. The combined effect of controlled atmosphere and melanosis inhibitors was used to delay black spot development as compared to the shrimp stored in ice alone. Storage under the controlled atmosphere without ice limited microbiological quality, namely, total viable counts, but enteroBacterial growth was lower.
P Montero - One of the best experts on this subject based on the ideXlab platform.
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effect of functional edible films and high pressure processing on microbial and oxidative spoilage in cold smoked sardine sardina pilchardus
Food Chemistry, 2007Co-Authors: Joaquin Gomezestaca, P Montero, B Gimenez, M C GomezguillenAbstract:Abstract The present experiment was an attempt to improve the shelf-life of cold-smoked sardine (Sardina pilchardus) using, singly or in combination, high pressure (300 MPa/20 °C/15 min) and gelatin-based functional edible films enriched by adding an extract of oregano (Origanum vulgare) or rosemary (Rosmarinus officinalis) or by adding chitosan. The uncoated muscle itself exhibited a certain level of antioxidant power (as measured by the FRAP method) ensuing from the deposition of phenols during smoking. Coating the muscle with the films enriched with the oregano or rosemary extracts increased the phenol content and the antioxidant power of the muscle, particularly when used in association with high pressure, due to migration of antioxidant substances from the film. The edible films with the added plant extracts lowered lipid oxidation levels (as measured by the peroxide and TBARS indices) and also, to a lesser extent, reduced microbial growth (total counts), whereas the gelatin–chitosan-based edible films lowered microbial counts (total counts, sulphide-reducing Bacteria). Neither Luminescent Bacteria nor EnteroBacteriaceae were detected in any of the batches. The combination of high pressure and edible films yielded the best results in terms of both preventing oxidation and inhibiting microbial growth.
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a 4 hexylresorcinol based formulation to prevent melanosis and microbial growth in chilled tiger prawns marsupenaeus japonicus from aquaculture
Journal of Food Science, 2006Co-Authors: Oscar Martinezalvarez, P Montero, M E Lopezcaballero, M C GomezguillenAbstract:A study was made of the effect on melanosis, biochemical indexes, and microbial growth in tiger prawns (Marsupenaeus japonicus) from aquaculture, using a formulation containing 4-hexylresorcinol (0.1% and 0.05%) in combination with organic acids (citric, ascorbic, and acetic) and chelating agents (ethylenediaminetetraacetic acid [EDTA] and disodium dihydrogen pyrophosphate [PPi]). In vivo and postmortem application of treatment was evaluated. Prawns with no additives or treated with 4% of a commercial formula based on sulfites were used to compare with 4-hexylresorcinol. The formulations based on 4-hexylresorcinol or sulfites inhibited the polyphenoloxidase (PPO) activity under 0.2 (Δoptical density [OD]/min/mL), instead of 1 (ΔOD/min/mL) achieved by prawns without additives, with the consequent delay in the appearance of melanosis during the 1st wk of storage. Prawns treated with sulfites showed initially better protection; however 4-hexylresorcinol proved to be more effective at the end of storage. The formulation based on 4-hexylresorcinol at 0.1% concentration, provided in vivo, inhibited the microbial growth (total Bacteria count, H2S-producer microorganisms, lactic acid Bacteria, enteroBacteria, and pseudomonads), whereas the commercial sulfites inhibited the Luminescent Bacteria growth.
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role of sulfites and 4 hexylresorcinol in microbial growth and melanosis prevention of deepwater pink shrimp parapenaeus longirostris using a controlled atmosphere
Journal of Food Protection, 2005Co-Authors: Oscar Martinezalvarez, M C Gomezguillen, P MonteroAbstract:A controlled atmosphere containing 48% CO2 and 7% O2 was used in association with refrigeration for storage of deepwater pink shrimp (Parapenaeus longirostris). Shrimp were treated with two different concentrations of sodium metabisulfite or 4-hexylresorcinol and subjected to the controlled atmosphere immediately after capture onboard ship or on arrival in port. Total volatile basic nitrogen, total viable counts, enteroBacteria, lactic acid Bacteria, and Luminescent Bacteria were determined, and black spot progression was evaluated. The combined effect of controlled atmosphere and melanosis inhibitors was used to delay black spot development as compared to the shrimp stored in ice alone. Storage under the controlled atmosphere without ice limited microbiological quality, namely, total viable counts, but enteroBacterial growth was lower.
