The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
Minghao Kong - One of the best experts on this subject based on the ideXlab platform.
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Role of transcriptional factor Nrf2 in the acute Lung injury of mice.
International Journal of Clinical and Experimental Pathology, 2015Co-Authors: Minghao KongAbstract:Objective: This study aimed to investigate the expression and role of Nrf2 in the acute Lung injury (ALI) of mice. Methods: A total of 60 BABL/c mice were randomly divided into 2 groups: ALI group and control group. In ALI group, ALI was introduced by injection of LPS. Immunohistochemistry was performed to detect Nrf2 expression in the Lung; Western blot assay was employed to detect the expression of Nrf2 in the Lung Homogenate; ELISA was conducted to detect the expression of Nrf2 in the Lung Homogenate and BALF. Results: As compared to control group, ALI mice had a high Nrf2 expression in the Lung as shown in immunohistochemistry, and the Nrf2 expression in the Lung Homogenate and BALF also increased markedly (P
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Original Article Role of transcriptional factor Nrf2 in the acute Lung injury of mice
2015Co-Authors: Minghao Kong, Jing Mao, Bijun Luo, Zonghao QinAbstract:* Equal contributors. Received July 23, 2015; Accepted August 26, 2015; Epub September 1, 2015; Published September 15, 2015 Abstract: Objective: This study aimed to investigate the expression and role of Nrf2 in the acute Lung injury (ALI) of mice. Methods: A total of 60 BABL/c mice were randomly divided into 2 groups: ALI group and control group. In ALI group, ALI was introduced by injection of LPS. Immunohistochemistry was performed to detect Nrf2 expression in the Lung; Western blot assay was employed to detect the expression of Nrf2 in the Lung Homogenate; ELISA was con- ducted to detect the expression of Nrf2 in the Lung Homogenate and BALF. Results: As compared to control group, ALI mice had a high Nrf2 expression in the Lung as shown in immunohistochemistry, and the Nrf2 expression in the Lung Homogenate and BALF also increased markedly (P
Xu Wen-ying - One of the best experts on this subject based on the ideXlab platform.
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Influence of pyrrolidine dithiocarbamate on the tumor necrosis factor-α in rats with acute Lung injury
Shandong Medical Journal, 2012Co-Authors: Xu Wen-yingAbstract:Objective To investigate the influence of pyrrolidine dithiocarbamate(PDTC) on the tumor necrosis factor-α in rats with acute Lung injury(ALI) induced by lipopolysaccharide(LPS).Methods A total of 66 male rats were randomly divided into 3 groups.The rats in control group(N group,n=6) were injected into the peritoneal cavity with normal saline,in Lung injury group(L group,n=30) with LPS to reproduce ALI model,and in PDTC group(P+L group,n=30) with LPS and PDTC.The samples of L group and P+L group were taken for TNF-α in Lung Homogenate and blood plasma at 1 h,2 h,4 h,8 h and 12 h.Result Compared with N group,TNF-α increased significantly in L group(P0.01) and was better in P+L group(P0.05).Conclusions LPS caused amount of TNF-α release in Lung Homogenate and blood.It suggestes that NF-κB involves in control of inflammation and plays an important role in ALI.PDTC can control the expression and release of inflammation,and effectively relieve the ALI induced by LPS.
Wei Mao - One of the best experts on this subject based on the ideXlab platform.
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The relationship of NO level and NOS bioactivity in Lung Homogenate and plasma of rats exposed to silica
Chinese Occupational Medicine, 2002Co-Authors: Wei MaoAbstract:Objective To study the relationships of nitric oxide (NO) level and nitric oxide synthase (NOS) bioactivity in Lung Homogenate and plasma of rats exposed to silica. Methods Factorial design was used in this study. Animal models were established by injecting silica (40mg per animal) directly into the Lung of rats through trachea. NO concentration was determined by nitric acid reductase method. NOS bioactivity was determined by NOS catalyzed L Arg. Data were analyzed by SPSS 8 0 and SAS 6 12. Results The correlations of LNO PNO and LNO PNOS in the experimental group were positive ( P =0 016, P =0 011). However, the correlation of LNOS PNO and LNOS PNOS didn't exist ( P 0 05). Conclusion Silica might cause the correlations of LNO PNO and LNO PNOS. The changes of the levels of NO, NOS in both plasma and Lungs in rats exposed to silica might have different mechanisms.
Grazyna Kwapiszewska - One of the best experts on this subject based on the ideXlab platform.
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Microarray analysis in pulmonary hypertension.
The European respiratory journal, 2016Co-Authors: Julia Hoffmann, Jochen Wilhelm, Andrea Olschewski, Grazyna KwapiszewskaAbstract:Microarrays are a powerful and effective tool that allows the detection of genome-wide gene expression differences between controls and disease conditions. They have been broadly applied to investigate the pathobiology of diverse forms of pulmonary hypertension, namely group 1, including patients with idiopathic pulmonary arterial hypertension, and group 3, including pulmonary hypertension associated with chronic Lung diseases such as chronic obstructive pulmonary disease and idiopathic pulmonary fibrosis. To date, numerous human microarray studies have been conducted to analyse global (Lung Homogenate samples), compartment-specific (laser capture microdissection), cell type-specific (isolated primary cells) and circulating cell (peripheral blood) expression profiles. Combined, they provide important information on development, progression and the end-stage disease. In the future, system biology approaches, expression of noncoding RNAs that regulate coding RNAs, and direct comparison between animal models and human disease might be of importance.
Ashim K. Mitra - One of the best experts on this subject based on the ideXlab platform.
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Pulmonary biotransformation of insulin in rat and rabbit.
Life sciences, 1992Co-Authors: Fang-yu Liu, Dane O. Kildsig, Ashim K. MitraAbstract:Abstract In vitro biodegradation of insulin in rabbit and rat Lung Homogenates was investigated. Insulin can be sequentially metabolized into two primary fragments in rabbit Lung Homogenate by an aminopeptidase. The amino acid sequences of the fragments were found to be the des-Phe-InsulinB1 (Metabolite I) and des-Phe-Val-InsulinB1−2 (Metabolite II). However, only the former metabolite (Metabolite I) was identified in the rat Lung Homogenate. The km and Vm values associated with rabbit Lung Homogenate were 0.29 ± 0.14 mM and 16.4 ± 6.9 μM/hr/mg protein, respectively, whereas those for a rabbit Lung preparation containing both microsomes and cytosol were 0.22 ± 0.07 mM and 17.9 ± 5.4 μM/hr/mg protein, respectively. The km and Vm associated with the cytosolic fraction of rabbit Lung were 0.32 ± 0.16 and 20.6 ± 6.1 μM/hr/mg protein, respectively. The results indicate that the Lung aminopeptidase may be a cytosolic enzyme. The degradation of dimeric insulin in the Lung Homogenate was faster than that of hexameric insulin due to the difference in collision frequency between the enzyme and insulin aggregates. The major metabolites in the Lungs reportedly retain almost the same bioactivity of insulin, suggesting that the pulmonary route of insulin delivery will not adversely affect its hypoglycemic activity.
