The Experts below are selected from a list of 50781 Experts worldwide ranked by ideXlab platform
Katsuhiro Hayashi - One of the best experts on this subject based on the ideXlab platform.
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Inhibition of spontaneous and experimental Lung Metastasis of soft-tissue sarcoma by tumor-targeting Salmonella typhimurium A1-R
Oncotarget, 2014Co-Authors: Shinji Miwa, Yong Zhang, Kyung-eun Baek, Fuminari Uehara, Shuya Yano, Mako Yamamoto, Yukihiko Hiroshima, Yasunori Matsumoto, Hiroaki Kimura, Katsuhiro HayashiAbstract:Prognosis of patients with Lung metastases of soft-tissue sarcoma is still poor. Therefore, novel systemic therapy is needed to improve the survival of soft-tissue sarcoma. In the present study, tumor-targeting therapy with a genetically-modified auxotrophic strain of Salmonella typhimurium, termed A1-R, was evaluated. Mouse models of primary soft tissue sarcoma and spontaneous Lung Metastasis were obtained by orthotopic intra-muscular injection of HT1080-RFP human fibrosarcoma cells. S. typhimurium A1-R was administered from day 14, once a week for two weeks. On day 28, Lung samples were excised and observed with a fluorescence imaging system. The number of Lung Metastasis was 8.8 ± 3.4 in the untreated group and 0.8 ± 0.8 in the treated group (P = 0.024). A mouse model of experimental Lung Metastasis was obtained by tail vein injection of HT1080-RFP cells. The mice were treated with S. typhimurium A1-R (i.v.) on day 7, once a week for three weeks. S. typhimurium A1-R significantly reduced Lung metastases and improved overall survival (P = 0.004). S. typhimurium A1-R bacterial therapy has future potential for treating advanced soft tissue sarcoma and improving prognosis of patients with Lung Metastasis.
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inhibition of spontaneous and experimental Lung Metastasis of soft tissue sarcoma by tumor targeting salmonella typhimurium a1 r
Oncotarget, 2014Co-Authors: Shinji Miwa, Yong Zhang, Kyung-eun Baek, Fuminari Uehara, Shuya Yano, Mako Yamamoto, Yukihiko Hiroshima, Yasunori Matsumoto, Hiroaki Kimura, Katsuhiro HayashiAbstract:// Shinji Miwa 1, 2, 3 , Yong Zhang 1, 2 , Kyung-Eun Baek 1 , Fuminari Uehara 1, 2 , Shuya Yano 1, 2 , Mako Yamamoto 1, 2 , Yukihiko Hiroshima 1, 2 , Yasunori Matsumoto 1, 2 , Hiroaki Kimura 3 , Katsuhiro Hayashi 3 , Norio Yamamoto 3 , Michael Bouvet 2 , Hiroyuki Tsuchiya 3 , Robert M. Hoffman 1, 2 , Ming Zhao 1 1 AntiCancer, Inc., San Diego, California, USA 2 Department of Surgery, University of California, San Diego, San Diego, California, USA 3 Department of Orthopedic Surgery, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Ishikawa, Japan Correspondence to: Robert M. Hoffman or Ming Zhao, e-mail: all@anticancer.com Keywords: HT-1080; orthotopic model; nude mice; Lung Metastasis; bacterial therapy Received: August 22, 2014 Accepted: October 01, 2014 Published: December 30, 2014 ABSTRACT Prognosis of patients with Lung metastases of soft-tissue sarcoma is still poor. Therefore, novel systemic therapy is needed to improve the survival of soft-tissue sarcoma. In the present study, tumor-targeting therapy with a genetically-modified auxotrophic strain of Salmonella typhimurium , termed A1-R, was evaluated. Mouse models of primary soft tissue sarcoma and spontaneous Lung Metastasis were obtained by orthotopic intra-muscular injection of HT1080-RFP human fibrosarcoma cells. S. typhimurium A1-R was administered from day 14, once a week for two weeks. On day 28, Lung samples were excised and observed with a fluorescence imaging system. The number of Lung Metastasis was 8.8 ± 3.4 in the untreated group and 0.8 ± 0.8 in the treated group ( P = 0.024). A mouse model of experimental Lung Metastasis was obtained by tail vein injection of HT1080-RFP cells. The mice were treated with S. typhimurium A1-R (i.v.) on day 7, once a week for three weeks. S. typhimurium A1-R significantly reduced Lung metastases and improved overall survival ( P = 0.004). S. typhimurium A1-R bacterial therapy has future potential for treating advanced soft tissue sarcoma and improving prognosis of patients with Lung Metastasis.
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systemic targeting of primary bone tumor and Lung Metastasis of high grade osteosarcoma in nude mice with a tumor selective strain of salmonella typhimurium
Cell Cycle, 2009Co-Authors: Katsuhiro Hayashi, Hiroyuki Tsuchiya, Michael Bouvet, Ming Zhao, Kensuke Yamauchi, Norio Yamamoto, Katsuro Tomita, Hiroyuki Kishimoto, Robert M HoffmanAbstract:We report here a new targeting strategy for primary bone tumor and Lung Metastasis with a modified auxotrophic strain of Salmonella typhimurium. We have previously developed the genetically-modified strain of S. typhimurium, selected for tumor targeting and therapy in vivo. Normal tissue is cleared of these bacteria even in immunodeficient athymic mice with no apparent side effects. In this study, the tumor-targeting strain of S. typhimurium, termed A1-R, was administered i.v. to nude mice which have primary bone tumor and Lung Metastasis. Primary bone tumor was obtained by orthotopic intra-tibial injection of 5 x 10(5) 143B-RFP (red fluorescent protein) human osteosarcoma cells. One group of mice was treated with A1-R expressing GFP (green fluorescent protein) and another group was used a as control. A1-R (5 x 10(7) colony-forming units) was injected in the tail vein three times on a weekly basis. On day 28, Lung samples were excised and observed with the Olympus OV100 Small Animal Imaging System. The size of the primary tumor and RFP intensity of Lung Metastasis were measured. Primary bone tumor size (fluorescence area [mm(2)]) was 232 +/- 70 in the untreated group and 95 +/- 23 in the treated group (p < 0.05). RFP intensity of the Lung Metastasis was 3 +/- 1.5 x 10(6) in the untreated group and 0.42 +/- 0.33 x 10(6) in the treated group (p < 0.05). Therefore, bacterial treatment was effective for both primary bone tumor and Lung Metastasis.
Shinji Miwa - One of the best experts on this subject based on the ideXlab platform.
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inhibition of soft tissue sarcoma Lung Metastasis by tumor targeting salmonella typhimurium a1 r
Journal of Clinical Oncology, 2015Co-Authors: Shinji Miwa, Yong Zhang, Hiroyuki Tsuchiya, Michael Bouvet, Robert M Hoffman, Ming ZhaoAbstract:e13515 Background: Prognosis of patients with Lung metastases of soft-tissue sarcoma is still poor, therefore, more effective therapeutics are needed. Methods: In the present study, tumor-targeting therapy with a genetically-modified auxotrophic strain of Salmonella typhimurium, termed A1-R, was evaluated on mouse models of primary soft tissue sarcoma where spontaneous Lung Metastasis occurred after orthotopic intra-muscular injection of HT1080-RFP human fibrosarcoma cells. S. typhimurium A1-R was administered from day 14, once a week for two weeks. On day 28, Lung samples were excised and observed with a fluorescence imaging system. A mouse model of experimental Lung Metastasis was obtained by tail vein injection of HT1080-RFP cells. The mice were treated with S. typhimurium A1-R (i.v.) on day 7, once a week for three weeks. Results: The number of spontaneous Lung Metastasis was 8.8 ± 3.4 in the untreated group and 0.8 ± 0.8 in the S. typhimurium A1-R treated group (P = 0.024). S. typhimurium A1-R also s...
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Inhibition of spontaneous and experimental Lung Metastasis of soft-tissue sarcoma by tumor-targeting Salmonella typhimurium A1-R
Oncotarget, 2014Co-Authors: Shinji Miwa, Yong Zhang, Kyung-eun Baek, Fuminari Uehara, Shuya Yano, Mako Yamamoto, Yukihiko Hiroshima, Yasunori Matsumoto, Hiroaki Kimura, Katsuhiro HayashiAbstract:Prognosis of patients with Lung metastases of soft-tissue sarcoma is still poor. Therefore, novel systemic therapy is needed to improve the survival of soft-tissue sarcoma. In the present study, tumor-targeting therapy with a genetically-modified auxotrophic strain of Salmonella typhimurium, termed A1-R, was evaluated. Mouse models of primary soft tissue sarcoma and spontaneous Lung Metastasis were obtained by orthotopic intra-muscular injection of HT1080-RFP human fibrosarcoma cells. S. typhimurium A1-R was administered from day 14, once a week for two weeks. On day 28, Lung samples were excised and observed with a fluorescence imaging system. The number of Lung Metastasis was 8.8 ± 3.4 in the untreated group and 0.8 ± 0.8 in the treated group (P = 0.024). A mouse model of experimental Lung Metastasis was obtained by tail vein injection of HT1080-RFP cells. The mice were treated with S. typhimurium A1-R (i.v.) on day 7, once a week for three weeks. S. typhimurium A1-R significantly reduced Lung metastases and improved overall survival (P = 0.004). S. typhimurium A1-R bacterial therapy has future potential for treating advanced soft tissue sarcoma and improving prognosis of patients with Lung Metastasis.
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inhibition of spontaneous and experimental Lung Metastasis of soft tissue sarcoma by tumor targeting salmonella typhimurium a1 r
Oncotarget, 2014Co-Authors: Shinji Miwa, Yong Zhang, Kyung-eun Baek, Fuminari Uehara, Shuya Yano, Mako Yamamoto, Yukihiko Hiroshima, Yasunori Matsumoto, Hiroaki Kimura, Katsuhiro HayashiAbstract:// Shinji Miwa 1, 2, 3 , Yong Zhang 1, 2 , Kyung-Eun Baek 1 , Fuminari Uehara 1, 2 , Shuya Yano 1, 2 , Mako Yamamoto 1, 2 , Yukihiko Hiroshima 1, 2 , Yasunori Matsumoto 1, 2 , Hiroaki Kimura 3 , Katsuhiro Hayashi 3 , Norio Yamamoto 3 , Michael Bouvet 2 , Hiroyuki Tsuchiya 3 , Robert M. Hoffman 1, 2 , Ming Zhao 1 1 AntiCancer, Inc., San Diego, California, USA 2 Department of Surgery, University of California, San Diego, San Diego, California, USA 3 Department of Orthopedic Surgery, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Ishikawa, Japan Correspondence to: Robert M. Hoffman or Ming Zhao, e-mail: all@anticancer.com Keywords: HT-1080; orthotopic model; nude mice; Lung Metastasis; bacterial therapy Received: August 22, 2014 Accepted: October 01, 2014 Published: December 30, 2014 ABSTRACT Prognosis of patients with Lung metastases of soft-tissue sarcoma is still poor. Therefore, novel systemic therapy is needed to improve the survival of soft-tissue sarcoma. In the present study, tumor-targeting therapy with a genetically-modified auxotrophic strain of Salmonella typhimurium , termed A1-R, was evaluated. Mouse models of primary soft tissue sarcoma and spontaneous Lung Metastasis were obtained by orthotopic intra-muscular injection of HT1080-RFP human fibrosarcoma cells. S. typhimurium A1-R was administered from day 14, once a week for two weeks. On day 28, Lung samples were excised and observed with a fluorescence imaging system. The number of Lung Metastasis was 8.8 ± 3.4 in the untreated group and 0.8 ± 0.8 in the treated group ( P = 0.024). A mouse model of experimental Lung Metastasis was obtained by tail vein injection of HT1080-RFP cells. The mice were treated with S. typhimurium A1-R (i.v.) on day 7, once a week for three weeks. S. typhimurium A1-R significantly reduced Lung metastases and improved overall survival ( P = 0.004). S. typhimurium A1-R bacterial therapy has future potential for treating advanced soft tissue sarcoma and improving prognosis of patients with Lung Metastasis.
Curzio Ruegg - One of the best experts on this subject based on the ideXlab platform.
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the matricellular protein cyr61 promotes breast cancer Lung Metastasis by facilitating tumor cell extravasation and suppressing anoikis
Oncotarget, 2017Co-Authors: Yuting Huang, Qiang Lan, Girieca Lorusso, Nathalie Duffey, Curzio RueggAbstract:// Yu-Ting Huang 1, 2, * , Qiang Lan 1, 2, * , Girieca Lorusso 1, 2 , Nathalie Duffey 1 , Curzio Ruegg 1, 2 1 Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland 2 National Center for Competence in Research (NCCR), Molecular Oncology, Swiss Institute for Experimental Cancer Research (ISREC)-Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, Switzerland * These authors contributed equally to this work Correspondence to: Curzio Ruegg, email: curzio.ruegg@unifr.ch Keywords: CYR61, Metastasis, extravasation, anoikis resistance Received: June 20, 2016 Accepted: November 19, 2016 Published: November 29, 2016 ABSTRACT Matricellular proteins play multiple roles in primary tumor growth, local invasion and tumor angiogenesis. However, their contribution to Metastasis and the putative mechanisms involved are less well characterized. In ER-negative human breast cancer, elevated expression levels of the matricellular protein Cysteine-rich angiogenic inducer 61 (CYR61) are associated with more aggressive progression. Here, we investigated the role of CYR61 in breast cancer Lung Metastasis using the triple negative human breast cancer cell lines MDA-MB-231 and SUM159. Silencing of CYR61 significantly decreased Lung Metastasis from tumors orthotopically implanted in pre-irradiated or naive mammary tissue and upon tail vein injection. Constitutive CYR61 silencing impaired cancer cell extravasation to the Lung during the first 24 hours after tail vein injection. In contrast, CYR61 inducible silencing starting 24 hours after cancer cell injection had no impact on Lung Metastasis formation. In vitro experiments revealed that CYR61 silencing decreased cancer cell transendothelial migration and motility, reduced CYR61 levels present at the cell surface and sensitized cancer cells to anoikis. Furthermore, we demonstrate that CYR61-dependent cell survival under non-adhesive conditions relied, at least partially, on β 1 integrin ligation and AMPKα signaling while it was independent of AKT, FAK and ERK1/2 activation. Our data provide the first evidence that CYR61 promotes breast cancer Lung Metastasis by facilitating tumor cell extravasation and protecting from anoikis during initial seeding to the Lung. The uncovered CYR61-β 1 integrin-AMPKα axis may serve as a potential therapeutic target to prevent breast cancer Metastasis to the Lung.
Yong Zhang - One of the best experts on this subject based on the ideXlab platform.
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inhibition of soft tissue sarcoma Lung Metastasis by tumor targeting salmonella typhimurium a1 r
Journal of Clinical Oncology, 2015Co-Authors: Shinji Miwa, Yong Zhang, Hiroyuki Tsuchiya, Michael Bouvet, Robert M Hoffman, Ming ZhaoAbstract:e13515 Background: Prognosis of patients with Lung metastases of soft-tissue sarcoma is still poor, therefore, more effective therapeutics are needed. Methods: In the present study, tumor-targeting therapy with a genetically-modified auxotrophic strain of Salmonella typhimurium, termed A1-R, was evaluated on mouse models of primary soft tissue sarcoma where spontaneous Lung Metastasis occurred after orthotopic intra-muscular injection of HT1080-RFP human fibrosarcoma cells. S. typhimurium A1-R was administered from day 14, once a week for two weeks. On day 28, Lung samples were excised and observed with a fluorescence imaging system. A mouse model of experimental Lung Metastasis was obtained by tail vein injection of HT1080-RFP cells. The mice were treated with S. typhimurium A1-R (i.v.) on day 7, once a week for three weeks. Results: The number of spontaneous Lung Metastasis was 8.8 ± 3.4 in the untreated group and 0.8 ± 0.8 in the S. typhimurium A1-R treated group (P = 0.024). S. typhimurium A1-R also s...
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Inhibition of spontaneous and experimental Lung Metastasis of soft-tissue sarcoma by tumor-targeting Salmonella typhimurium A1-R
Oncotarget, 2014Co-Authors: Shinji Miwa, Yong Zhang, Kyung-eun Baek, Fuminari Uehara, Shuya Yano, Mako Yamamoto, Yukihiko Hiroshima, Yasunori Matsumoto, Hiroaki Kimura, Katsuhiro HayashiAbstract:Prognosis of patients with Lung metastases of soft-tissue sarcoma is still poor. Therefore, novel systemic therapy is needed to improve the survival of soft-tissue sarcoma. In the present study, tumor-targeting therapy with a genetically-modified auxotrophic strain of Salmonella typhimurium, termed A1-R, was evaluated. Mouse models of primary soft tissue sarcoma and spontaneous Lung Metastasis were obtained by orthotopic intra-muscular injection of HT1080-RFP human fibrosarcoma cells. S. typhimurium A1-R was administered from day 14, once a week for two weeks. On day 28, Lung samples were excised and observed with a fluorescence imaging system. The number of Lung Metastasis was 8.8 ± 3.4 in the untreated group and 0.8 ± 0.8 in the treated group (P = 0.024). A mouse model of experimental Lung Metastasis was obtained by tail vein injection of HT1080-RFP cells. The mice were treated with S. typhimurium A1-R (i.v.) on day 7, once a week for three weeks. S. typhimurium A1-R significantly reduced Lung metastases and improved overall survival (P = 0.004). S. typhimurium A1-R bacterial therapy has future potential for treating advanced soft tissue sarcoma and improving prognosis of patients with Lung Metastasis.
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inhibition of spontaneous and experimental Lung Metastasis of soft tissue sarcoma by tumor targeting salmonella typhimurium a1 r
Oncotarget, 2014Co-Authors: Shinji Miwa, Yong Zhang, Kyung-eun Baek, Fuminari Uehara, Shuya Yano, Mako Yamamoto, Yukihiko Hiroshima, Yasunori Matsumoto, Hiroaki Kimura, Katsuhiro HayashiAbstract:// Shinji Miwa 1, 2, 3 , Yong Zhang 1, 2 , Kyung-Eun Baek 1 , Fuminari Uehara 1, 2 , Shuya Yano 1, 2 , Mako Yamamoto 1, 2 , Yukihiko Hiroshima 1, 2 , Yasunori Matsumoto 1, 2 , Hiroaki Kimura 3 , Katsuhiro Hayashi 3 , Norio Yamamoto 3 , Michael Bouvet 2 , Hiroyuki Tsuchiya 3 , Robert M. Hoffman 1, 2 , Ming Zhao 1 1 AntiCancer, Inc., San Diego, California, USA 2 Department of Surgery, University of California, San Diego, San Diego, California, USA 3 Department of Orthopedic Surgery, Kanazawa University Graduate School of Medical Sciences, Kanazawa, Ishikawa, Japan Correspondence to: Robert M. Hoffman or Ming Zhao, e-mail: all@anticancer.com Keywords: HT-1080; orthotopic model; nude mice; Lung Metastasis; bacterial therapy Received: August 22, 2014 Accepted: October 01, 2014 Published: December 30, 2014 ABSTRACT Prognosis of patients with Lung metastases of soft-tissue sarcoma is still poor. Therefore, novel systemic therapy is needed to improve the survival of soft-tissue sarcoma. In the present study, tumor-targeting therapy with a genetically-modified auxotrophic strain of Salmonella typhimurium , termed A1-R, was evaluated. Mouse models of primary soft tissue sarcoma and spontaneous Lung Metastasis were obtained by orthotopic intra-muscular injection of HT1080-RFP human fibrosarcoma cells. S. typhimurium A1-R was administered from day 14, once a week for two weeks. On day 28, Lung samples were excised and observed with a fluorescence imaging system. The number of Lung Metastasis was 8.8 ± 3.4 in the untreated group and 0.8 ± 0.8 in the treated group ( P = 0.024). A mouse model of experimental Lung Metastasis was obtained by tail vein injection of HT1080-RFP cells. The mice were treated with S. typhimurium A1-R (i.v.) on day 7, once a week for three weeks. S. typhimurium A1-R significantly reduced Lung metastases and improved overall survival ( P = 0.004). S. typhimurium A1-R bacterial therapy has future potential for treating advanced soft tissue sarcoma and improving prognosis of patients with Lung Metastasis.
Ming Zhao - One of the best experts on this subject based on the ideXlab platform.
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inhibition of soft tissue sarcoma Lung Metastasis by tumor targeting salmonella typhimurium a1 r
Journal of Clinical Oncology, 2015Co-Authors: Shinji Miwa, Yong Zhang, Hiroyuki Tsuchiya, Michael Bouvet, Robert M Hoffman, Ming ZhaoAbstract:e13515 Background: Prognosis of patients with Lung metastases of soft-tissue sarcoma is still poor, therefore, more effective therapeutics are needed. Methods: In the present study, tumor-targeting therapy with a genetically-modified auxotrophic strain of Salmonella typhimurium, termed A1-R, was evaluated on mouse models of primary soft tissue sarcoma where spontaneous Lung Metastasis occurred after orthotopic intra-muscular injection of HT1080-RFP human fibrosarcoma cells. S. typhimurium A1-R was administered from day 14, once a week for two weeks. On day 28, Lung samples were excised and observed with a fluorescence imaging system. A mouse model of experimental Lung Metastasis was obtained by tail vein injection of HT1080-RFP cells. The mice were treated with S. typhimurium A1-R (i.v.) on day 7, once a week for three weeks. Results: The number of spontaneous Lung Metastasis was 8.8 ± 3.4 in the untreated group and 0.8 ± 0.8 in the S. typhimurium A1-R treated group (P = 0.024). S. typhimurium A1-R also s...
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systemic targeting of primary bone tumor and Lung Metastasis of high grade osteosarcoma in nude mice with a tumor selective strain of salmonella typhimurium
Cell Cycle, 2009Co-Authors: Katsuhiro Hayashi, Hiroyuki Tsuchiya, Michael Bouvet, Ming Zhao, Kensuke Yamauchi, Norio Yamamoto, Katsuro Tomita, Hiroyuki Kishimoto, Robert M HoffmanAbstract:We report here a new targeting strategy for primary bone tumor and Lung Metastasis with a modified auxotrophic strain of Salmonella typhimurium. We have previously developed the genetically-modified strain of S. typhimurium, selected for tumor targeting and therapy in vivo. Normal tissue is cleared of these bacteria even in immunodeficient athymic mice with no apparent side effects. In this study, the tumor-targeting strain of S. typhimurium, termed A1-R, was administered i.v. to nude mice which have primary bone tumor and Lung Metastasis. Primary bone tumor was obtained by orthotopic intra-tibial injection of 5 x 10(5) 143B-RFP (red fluorescent protein) human osteosarcoma cells. One group of mice was treated with A1-R expressing GFP (green fluorescent protein) and another group was used a as control. A1-R (5 x 10(7) colony-forming units) was injected in the tail vein three times on a weekly basis. On day 28, Lung samples were excised and observed with the Olympus OV100 Small Animal Imaging System. The size of the primary tumor and RFP intensity of Lung Metastasis were measured. Primary bone tumor size (fluorescence area [mm(2)]) was 232 +/- 70 in the untreated group and 95 +/- 23 in the treated group (p < 0.05). RFP intensity of the Lung Metastasis was 3 +/- 1.5 x 10(6) in the untreated group and 0.42 +/- 0.33 x 10(6) in the treated group (p < 0.05). Therefore, bacterial treatment was effective for both primary bone tumor and Lung Metastasis.
