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Zhiping Gu - One of the best experts on this subject based on the ideXlab platform.
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effects of droloxifene on apoptosis and bax bcl 2 protein expression of Luteal Cells in pseudopregnant rats
Acta Pharmacologica Sinica, 2001Co-Authors: Ying Leng, Ying Feng, Zhiping GuAbstract:Aim: To study the effects of droloxifene on apoptosis and the expression of Bax and Bcl-2 protein in corpus luteum of pseudopregnant rats. Methods: HE staining was used to examine the histological changes of ovaries. Apoptosis detection in situ was performed with TUNEL method. Expression of Bax and Bcl-2 protein was observed by immunohistochemistry analysis. Results: Apoptosis of Luteal Cells during the spontaneous regression of corpus luteum of pseudopregnant rats appeared on d 13 of pseudopregnancy, and the marked increase of apoptotic Luteal Cells could be observed on d 15. When pseudopregnant rats were treated with droloxifene 20 mg . Kg on d 2, apoptosis of Luteal Cells could be observed on d 8 and the duration of pseudopregnancy could be shortened from (15.5 +\- 1.1) d to (12.8 +\- 1.6) d. In pseudopregnant rats, the expression of Bax and Bcl-2 protein was found in the cytoplasm of Luteal Cells. However, no obvious differences in the intensity or localization could be found during various days of the pseudopregnancy, while an increase in Bax and a decrease in Bcl-2 protein expression could be induced by droloxifene treatment. Conclusion: Droloxifene could facilitate apoptosis of Luteal Cells in pseudopregnant rats and shorten the period of pseudopregnancy. An increased Bax/Bcl-2 ratio might be involved in the facilitation of apoptosis induced by droloxifene in corpus luteum of pseudopregnant rats.
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effects of droloxifene on apoptosis and bax bcl 2 protein expression of Luteal Cells in pseudopregnant rats
Acta Pharmacologica Sinica, 2001Co-Authors: Ying Leng, Zhiping GuAbstract:Aim: To study the effects of droloxifene on apoptosis and the expression of Bax and Bcl-2 protein in corpus luteum of pseudopregnant rats. Methods: HE staining was used to examine the histological changes of ovaries. Apoptosis detection in situ was performed with TUNEL method. Expression of Bax and Bcl-2 protein was observed by immunohistochemistry analysis. Results: Apoptosis of Luteal Cells during the spontaneous regression of corpus luteum of pseudopregnant rats appeared on d 13 of pseudopregnancy, and the marked increase of apoptotic Luteal Cells could be observed on d 15. When pseudopregnant rats were treated with droloxifene 20 mg . Kg on d 2, apoptosis of Luteal Cells could be observed on d 8 and the duration of pseudopregnancy could be shortened from (15.5 +\- 1.1) d to (12.8 +\- 1.6) d. In pseudopregnant rats, the expression of Bax and Bcl-2 protein was found in the cytoplasm of Luteal Cells. However, no obvious differences in the intensity or localization could be found during various days of the pseudopregnancy, while an increase in Bax and a decrease in Bcl-2 protein expression could be induced by droloxifene treatment. Conclusion: Droloxifene could facilitate apoptosis of Luteal Cells in pseudopregnant rats and shorten the period of pseudopregnancy. An increased Bax/Bcl-2 ratio might be involved in the facilitation of apoptosis induced by droloxifene in corpus luteum of pseudopregnant rats.
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apoptosis induced by droloxifene and c myc bax and bcl 2 mrna expression in cultured Luteal Cells of rats
European Journal of Pharmacology, 2000Co-Authors: Ying Leng, Zhiping GuAbstract:Abstract Droloxifene is a tamoxifen derivative whose effects in the therapy of human breast cancer and postmenopausal osteoporosis have been studied widely. We had found that droloxifene could induce apoptosis of Luteal Cells of rat in vitro, but its mechanisms were unknown. In the present study, the expression of c-myc, bax and bcl-2 mRNA in cultured rat Luteal Cells during apoptosis induced by droloxifene was investigated and possible associations between these genes and apoptosis were analyzed. Cultured Luteal Cells of rats were incubated with droloxifene at various concentrations and with treatment durations. Occurrence of apoptosis was detected by terminal deoxyribonucleotidyl tranferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL), DNA staining and DNA electrophoresis. Expression of these genes' mRNA was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the c-myc and bax mRNA levels increased as concentrations or treatment durations of droloxifene increased, while the bcl-2 mRNA level exhibited no changes. A marked increase of c-myc and bax mRNA appeared respectively with 12 and 24 h of treatment, while a clear increase of apoptosis of Luteal Cells was found at 18 h. These results suggested that droloxifene could induce apoptosis of Luteal Cells of rat in vitro. The increase of c-myc mRNA expression might be one of the initiating factors and the elevated ratio of bax/bcl-2 mRNA was also probably involved in this effect.
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apoptosis induced by droloxifene and c myc bax and bcl 2 mrna expression in cultured Luteal Cells of rats
European Journal of Pharmacology, 2000Co-Authors: Ying Leng, Zhiping GuAbstract:Abstract Droloxifene is a tamoxifen derivative whose effects in the therapy of human breast cancer and postmenopausal osteoporosis have been studied widely. We had found that droloxifene could induce apoptosis of Luteal Cells of rat in vitro, but its mechanisms were unknown. In the present study, the expression of c-myc, bax and bcl-2 mRNA in cultured rat Luteal Cells during apoptosis induced by droloxifene was investigated and possible associations between these genes and apoptosis were analyzed. Cultured Luteal Cells of rats were incubated with droloxifene at various concentrations and with treatment durations. Occurrence of apoptosis was detected by terminal deoxyribonucleotidyl tranferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL), DNA staining and DNA electrophoresis. Expression of these genes' mRNA was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the c-myc and bax mRNA levels increased as concentrations or treatment durations of droloxifene increased, while the bcl-2 mRNA level exhibited no changes. A marked increase of c-myc and bax mRNA appeared respectively with 12 and 24 h of treatment, while a clear increase of apoptosis of Luteal Cells was found at 18 h. These results suggested that droloxifene could induce apoptosis of Luteal Cells of rat in vitro. The increase of c-myc mRNA expression might be one of the initiating factors and the elevated ratio of bax/bcl-2 mRNA was also probably involved in this effect.
Joy L. Pate - One of the best experts on this subject based on the ideXlab platform.
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effects of concanavalin a on the progesterone production by bovine steroidogenic Luteal Cells in vitro
Reproduction in Domestic Animals, 2016Co-Authors: Flavia Caroline Destro, I Martin, Fernanda Da Cruz Landimalvarenga, J C P Ferreira, Joy L. PateAbstract:Contents The aim of this study was to evaluate the effects of concanavalin A (CONA) on the progesterone (P4) production by bovine steroidogenic Luteal Cells (LCs) in vitro. Luteal Cells were collected during the mid-Luteal stage (at 10–12 days following ovulation) and processed in the laboratory. Luteal Cells were grown for 7 days in a humid atmosphere with 5% CO2, with or without 10% foetal bovine serum, and were subjected to the following treatments: control: no treatment; CONA (10 μg/ml); LH (100 μg/ml); CONA + LH; LH (100 μg/ml) + prostaglandin F2α (PGF2α) (10 ng/ml); CONA + LH + PGF2α. Samples of the culture media were collected on days 1 (D1) and 7 (D7) for P4 quantification. The Cells were counted on D7 of culture. Differences between treatments were considered statistically significant at p < .05. Culture in the presence of CONA decreased the P4-secreting capacity of LCs on D7 of culture, particularly in the absence of serum. The cell numbers did not change between treatments.
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Luteal Cells from functional and regressing bovine corpora lutea differentially alter the function of gamma delta t Cells
Biology of Reproduction, 2014Co-Authors: Sadhat S. Walusimbi, Joy L. PateAbstract:ABSTRACT The Luteal microenvironment is thought to direct the function of resident immune Cells to facilitate either Luteal function or regression. To determine if Luteal Cells from functional (Days 10–12) and regressing (8 h after administration of prostaglandin F2alpha) corpora lutea (CL) induce different responses in γδ T Cells, Luteal Cells were cocultured with autologous γδ T Cells isolated from peripheral blood. Proliferation, functional phenotypes, and cytokine synthesis were analyzed by flow cytometry. To determine if the Luteal Cells from functional CL induce hyporesponsiveness in γδ T Cells, γδ+ Cells were cocultured with midcycle Luteal Cells and further stimulated with concanavalin A. Coculture of γδ+ Cells with midcycle Luteal Cells did not inhibit concanavalin A-induced proliferation. In a proliferation assay, Luteal Cells from midcycle CL predominantly induced proliferation of γδ+ WC1− Cells (P < 0.05), while Luteal Cells from regressing CL predominantly induced proliferation of γδ+WC1+ cel...
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Short title: Luteal Cells modulate the function of γδ T Cells
2014Co-Authors: Sadhat S. Walusimbi, Joy L. PateAbstract:The Luteal microenvironment is thought to direct the function of resident immune Cells to facilitate either Luteal function or regression. To determine if Luteal Cells from functional (Days 10-12) and regressing (8 h after administration of prostaglandin [PG] F2α) corpora lutea (CL) induce different responses in γδ T Cells, Luteal Cells were cocultured with autologous γδ T Cells isolated from peripheral blood. Proliferation, functional phenotypes and cytokine synthesis were analyzed by flow cytometry. To determine if the Luteal Cells from functional CL induce hyporesponsiveness in γδ T Cells, γδ + Cells were cocultured with midcycle Luteal Cells and further stimulated with concanavalin A (ConA). Coculture of γδ + Cells with midcycle Luteal Cells did not inhibit ConA-induced proliferation. In a proliferation assay, Luteal Cells from midcycle CL predominantly induced proliferation of γδ + WC1 - Cells (P < 0.05), while Luteal Cells from regressing CL predominantly induced proliferation of γδ + WC1 + Cells (P < 0.05). Analysis of intracellular cytokines indicated that midcycle Luteal Cells increased the proportion of γδ + Cells containing interleukin (IL) 10 (P < 0.05), but reduced the proportion of γδ + Cells containing interferon gamma (IFNG; P < 0.05). There were no changes in the proportions of γδ + Cells synthesizing interleukin IL4 or tumor necrosis factor (TNF). Unexpectedly, coculture of γδ + Cells with Luteal Cells from regressing CL had no effect on any of the cytokines analyzed. These data support the hypothesis that the function of resident T Cells is differentially modulated depending on the status of the CL. Summary sentence: Luteal Cells induce changes in γδ T cell function, but these changes are dependent on the type of T cell and the functional status of the corpus luteum.
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Bovine Luteal Cells Stimulate Proliferation of Major Histocompatibility Nonrestricted Gamma Delta T Cells
Biology of reproduction, 2007Co-Authors: Tracy L. Davis, Joy L. PateAbstract:Abstract Luteal Cells are potent activators of T cell proliferation in vitro. The purpose of this study was to determine which subset of T Cells is stimulated by Luteal Cells and whether Luteal cell-induced T cell activation elicits a proinflammatory or anti-inflammatory T cell response. The first objective was to determine if Luteal cell-stimulated T cell proliferation was mediated by class I or II major histocompatibility complex (MHC) molecules. T cell proliferation was inhibited by anti-MHC class I but not anti-MHC class II antibodies. The second objective was to determine which T cell subtype proliferates when cultured with Luteal Cells. The proportions of CD4+ and CD8+ Cells were unchanged, but the number of gamma delta T Cells was increased by coculture with Luteal Cells. Immunohistochemistry confirmed the presence of gamma delta T Cells in midcycle and regressing corpus luteum. The final objective was to characterize T cell cytokine production stimulated by Luteal Cells. The concentrations of inte...
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Effects of Prostaglandin F2α and Progesterone on the Ability of Bovine Luteal Cells to Stimulate T Lymphocyte Proliferation
Biology of reproduction, 2003Co-Authors: Matthew J. Cannon, Margaret G. Petroff, Joy L. PateAbstract:Bovine Luteal Cells express class I and II major histocompatibility complex molecules and stimulate T lymphocyte proliferation in vitro. Proliferation of T lymphocytes is greater in cocultures of Luteal Cells and T lymphocytes collected following administration of a luteolytic dose of prostaglandin (PG) F2a to the cow. Whether this results from changes in Luteal Cells that increase their ability to stimulate T lymphocyte proliferation or from changes in T lymphocytes that enhance their ability to respond to Luteal Cells is unclear. To determine which is the case, Luteal cell-T lymphocyte cocultures were performed using Luteal Cells and T lymphocytes isolated from the same animals before and 8 h after administration of PGF2a. In the presence of T lymphocytes collected before PGF2a administration, Luteal Cells isolated after PGF2a were more potent stimulators of T lymphocyte proliferation than were Luteal Cells collected before PGF2a (P , 0.05). The effect of progesterone on Luteal cell-stimulated T lymphocyte proliferation was also evaluated. Proliferation of T lymphocytes was greater (P , 0.05) in cultures containing the cytochrome P450 side-chain cleavage enzyme-inhibitor aminoglutethimide. Exogenous progesterone caused a dose-dependent inhibition of Luteal cell-stimulated T lymphocyte proliferation (P , 0.05). Progesterone-receptor mRNA was undetectable in peripheral blood mononuclear Cells collected before and after PGF2a administration, indicating that the effect of progesterone was not mediated via progesterone receptors in lymphocytes. These results imply that specific changes in Luteal Cells in response to PGF2a enhance the ability of these Cells to stimulate T lymphocyte proliferation. These results also demonstrate that progesterone can suppress Luteal cell-stimulated T lymphocyte proliferation. corpus luteum, immunology, ovary, progesterone, progesterone receptor
Ying Leng - One of the best experts on this subject based on the ideXlab platform.
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effects of droloxifene on apoptosis and bax bcl 2 protein expression of Luteal Cells in pseudopregnant rats
Acta Pharmacologica Sinica, 2001Co-Authors: Ying Leng, Ying Feng, Zhiping GuAbstract:Aim: To study the effects of droloxifene on apoptosis and the expression of Bax and Bcl-2 protein in corpus luteum of pseudopregnant rats. Methods: HE staining was used to examine the histological changes of ovaries. Apoptosis detection in situ was performed with TUNEL method. Expression of Bax and Bcl-2 protein was observed by immunohistochemistry analysis. Results: Apoptosis of Luteal Cells during the spontaneous regression of corpus luteum of pseudopregnant rats appeared on d 13 of pseudopregnancy, and the marked increase of apoptotic Luteal Cells could be observed on d 15. When pseudopregnant rats were treated with droloxifene 20 mg . Kg on d 2, apoptosis of Luteal Cells could be observed on d 8 and the duration of pseudopregnancy could be shortened from (15.5 +\- 1.1) d to (12.8 +\- 1.6) d. In pseudopregnant rats, the expression of Bax and Bcl-2 protein was found in the cytoplasm of Luteal Cells. However, no obvious differences in the intensity or localization could be found during various days of the pseudopregnancy, while an increase in Bax and a decrease in Bcl-2 protein expression could be induced by droloxifene treatment. Conclusion: Droloxifene could facilitate apoptosis of Luteal Cells in pseudopregnant rats and shorten the period of pseudopregnancy. An increased Bax/Bcl-2 ratio might be involved in the facilitation of apoptosis induced by droloxifene in corpus luteum of pseudopregnant rats.
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effects of droloxifene on apoptosis and bax bcl 2 protein expression of Luteal Cells in pseudopregnant rats
Acta Pharmacologica Sinica, 2001Co-Authors: Ying Leng, Zhiping GuAbstract:Aim: To study the effects of droloxifene on apoptosis and the expression of Bax and Bcl-2 protein in corpus luteum of pseudopregnant rats. Methods: HE staining was used to examine the histological changes of ovaries. Apoptosis detection in situ was performed with TUNEL method. Expression of Bax and Bcl-2 protein was observed by immunohistochemistry analysis. Results: Apoptosis of Luteal Cells during the spontaneous regression of corpus luteum of pseudopregnant rats appeared on d 13 of pseudopregnancy, and the marked increase of apoptotic Luteal Cells could be observed on d 15. When pseudopregnant rats were treated with droloxifene 20 mg . Kg on d 2, apoptosis of Luteal Cells could be observed on d 8 and the duration of pseudopregnancy could be shortened from (15.5 +\- 1.1) d to (12.8 +\- 1.6) d. In pseudopregnant rats, the expression of Bax and Bcl-2 protein was found in the cytoplasm of Luteal Cells. However, no obvious differences in the intensity or localization could be found during various days of the pseudopregnancy, while an increase in Bax and a decrease in Bcl-2 protein expression could be induced by droloxifene treatment. Conclusion: Droloxifene could facilitate apoptosis of Luteal Cells in pseudopregnant rats and shorten the period of pseudopregnancy. An increased Bax/Bcl-2 ratio might be involved in the facilitation of apoptosis induced by droloxifene in corpus luteum of pseudopregnant rats.
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apoptosis induced by droloxifene and c myc bax and bcl 2 mrna expression in cultured Luteal Cells of rats
European Journal of Pharmacology, 2000Co-Authors: Ying Leng, Zhiping GuAbstract:Abstract Droloxifene is a tamoxifen derivative whose effects in the therapy of human breast cancer and postmenopausal osteoporosis have been studied widely. We had found that droloxifene could induce apoptosis of Luteal Cells of rat in vitro, but its mechanisms were unknown. In the present study, the expression of c-myc, bax and bcl-2 mRNA in cultured rat Luteal Cells during apoptosis induced by droloxifene was investigated and possible associations between these genes and apoptosis were analyzed. Cultured Luteal Cells of rats were incubated with droloxifene at various concentrations and with treatment durations. Occurrence of apoptosis was detected by terminal deoxyribonucleotidyl tranferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL), DNA staining and DNA electrophoresis. Expression of these genes' mRNA was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the c-myc and bax mRNA levels increased as concentrations or treatment durations of droloxifene increased, while the bcl-2 mRNA level exhibited no changes. A marked increase of c-myc and bax mRNA appeared respectively with 12 and 24 h of treatment, while a clear increase of apoptosis of Luteal Cells was found at 18 h. These results suggested that droloxifene could induce apoptosis of Luteal Cells of rat in vitro. The increase of c-myc mRNA expression might be one of the initiating factors and the elevated ratio of bax/bcl-2 mRNA was also probably involved in this effect.
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apoptosis induced by droloxifene and c myc bax and bcl 2 mrna expression in cultured Luteal Cells of rats
European Journal of Pharmacology, 2000Co-Authors: Ying Leng, Zhiping GuAbstract:Abstract Droloxifene is a tamoxifen derivative whose effects in the therapy of human breast cancer and postmenopausal osteoporosis have been studied widely. We had found that droloxifene could induce apoptosis of Luteal Cells of rat in vitro, but its mechanisms were unknown. In the present study, the expression of c-myc, bax and bcl-2 mRNA in cultured rat Luteal Cells during apoptosis induced by droloxifene was investigated and possible associations between these genes and apoptosis were analyzed. Cultured Luteal Cells of rats were incubated with droloxifene at various concentrations and with treatment durations. Occurrence of apoptosis was detected by terminal deoxyribonucleotidyl tranferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL), DNA staining and DNA electrophoresis. Expression of these genes' mRNA was determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the c-myc and bax mRNA levels increased as concentrations or treatment durations of droloxifene increased, while the bcl-2 mRNA level exhibited no changes. A marked increase of c-myc and bax mRNA appeared respectively with 12 and 24 h of treatment, while a clear increase of apoptosis of Luteal Cells was found at 18 h. These results suggested that droloxifene could induce apoptosis of Luteal Cells of rat in vitro. The increase of c-myc mRNA expression might be one of the initiating factors and the elevated ratio of bax/bcl-2 mRNA was also probably involved in this effect.
Yasunori Yoshimura - One of the best experts on this subject based on the ideXlab platform.
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participation of reactive oxygen species in pgf2alpha induced apoptosis in rat Luteal Cells
Reproduction, 2000Co-Authors: Mamoru Tanaka, Toyohiko Miyazaki, Shinji Tanigaki, Kenji Kasai, Kazuhiro Minegishi, Kei Miyakoshi, Hitoshi Ishimoto, Yasunori YoshimuraAbstract:Prostaglandin F(2alpha) (PGF(2alpha)) is implicated in the process of Luteal regression in many species. Treatment of rat Luteal tissue with PGF(2alpha) increases the generation of reactive oxygen species. Since reactive oxygen species have been implicated in apoptosis, the present study was undertaken to determine whether reactive oxygen species play a role in the PGF(2alpha)-induced apoptosis of rat Luteal Cells. Rat Luteal Cells were loaded with 6-carboxy-2, 7'-dichlorodihydro-fluorescein (CDCFH) diacetate, di (acetomethyl ester), which can be oxidized by reactive oxygen species to yield CDCF, a fluorescent molecule, and the Cells were treated with different doses of PGF(2alpha). Incubation with 100 micromol PGF(2alpha) l(-1) induced an increase in CDCF fluorescence (P < 0. 05). Treatment of Cells with PGF(2alpha) for 48 h in serum-free medium induced a dose-dependent increase in cell death, and these Cells exhibited the morphological characteristics typical of apoptosis, including condensed or fragmented nuclei and fragmentation of internucleosomal DNA. Pretreatment of these Cells with ascorbic acid, N,N'-dimethylthiourea, or superoxide dismutase, which acts as an antioxidant or a radical scavenger, prevented the PGF(2alpha)-induced apoptosis. These results demonstrate that PGF(2alpha) produces reactive oxygen species and induces apoptosis in rat Luteal Cells, indicating that the reactive oxygen species may induce apoptotic cell death during luteolysis.
Izabela Woclawek-potocka - One of the best experts on this subject based on the ideXlab platform.
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Lysophosphatidic acid modulates prostaglandin signalling in bovine steroidogenic Luteal Cells.
Prostaglandins & other lipid mediators, 2015Co-Authors: Ilona Kowalczyk-zieba, Dorota Boruszewska, Emilia Sinderewicz, Katarzyna Grycmacher, Izabela Woclawek-potockaAbstract:We examined whether lysophosphatidic acid affects prostaglandin biosynthesis, transport, and signalling in bovine steroidogenic Luteal Cells. The aim of the present study was to determine the influence of LPA on PGE2 and PGF2α synthesis and on the expression of enzymes involved in PG biosynthesis (PTGS2, mPGES-1, cPGES, mPGES-2, PGFS and 9-KPR), prostaglandin transporter (PGT), and prostaglandin receptors (EP1, EP2, EP3, EP4 and FP) in bovine steroidogenic Luteal Cells. We found that LPA inhibited PGF2α synthesis in steroidogenic Luteal Cells. Moreover, LPA increased mPGES1 and cPGES and decreased PGFS expression in cultured bovine steroidogenic Luteal Cells. Additionally, LPA stimulated EP2 and EP4 receptor and PGT expression. This study suggests that LPA activity in the bovine CL directs the physiological intraLuteal balance between the two main prostanoids towards luteotropic PGE2.
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Influence of Lysophosphatidic Acid on Nitric Oxide-Induced Luteolysis in Steroidogenic Luteal Cells in Cows
Biology of Reproduction, 2014Co-Authors: Ilona Kowalczyk-zieba, Dorota Boruszewska, Emilia Sinderewicz, Dariusz J Skarzynski, Izabela Woclawek-potockaAbstract:ABSTRACT Lysophosphatidic acid (LPA) together with its active G protein-coupled receptors are present in the corpus luteum (CL) of the cow. Under in vivo conditions, LPA stimulated P4 and PGE2 secretion during the Luteal phase of the estrous cycle in heifers. Furthermore, LPA maintained P4 synthesis and actions in the bovine CL in vitro. However, the effect of this phospholipid on nitric oxide (NO)-induced functional and structural luteolysis has not been investigated. The aim of the present work was to determine the effects of LPA on 1) NO-induced functional luteolysis, 2) NO-dependent PG synthesis, and 3) NO-induced structural luteolysis in cultured steroidogenic Luteal Cells. We documented that LPA reversed the inhibitory effect of NONOate, an NO donor, on P4 synthesis and PGE2/PGF2alpha ratio in cultured steroidogenic Luteal Cells. Additionally, LPA inhibited NO-induced apoptosis in cultured steroidogenic Luteal Cells via abrogation of the NO-dependent stimulatory influence on proapoptotic TNFalpha/TN...
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Progesterone Is a Suppressor of Apoptosis in Bovine Luteal Cells
Biology of reproduction, 2004Co-Authors: Kiyoshi Okuda, Ryo Nishimura, Izabela Woclawek-potocka, Masami Shibaya, Anna Korzekwa, Shuko Murakami, Miki Tsubouchi, Dariusz J SkarzynskiAbstract:Abstract Progesterone is suggested to be a suppressor of apoptosis in bovine Luteal Cells. Fas antigen (Fas) is a cell surface receptor that triggers apoptosis in sensitive Cells. Furthermore, apoptosis is known to be controlled by the bcl-2 gene/protein family and caspases. This study was undertaken to determine whether intraLuteal progesterone (P4) is involved in Fas L–mediated Luteal cell death in the bovine corpus luteum (CL) in vitro. Moreover, we studied whether an antagonist of P4 influences gene expression of the bcl-2 family and caspase-3 and the activity of caspase-3 in the bovine CL. Luteal Cells obtained from the cows in the midLuteal phase of the estrous cycle (Days 8–12 of the cycle) were exposed to a specific P4 antagonist (onapristone [OP], 10−4 M) with or without 100 ng/ml Fas L. Although Fas L alone did not show a cytotoxic effect, treatment of the Cells with OP alone or in combination with Fas L resulted in killing of 30% and 45% of the Cells, respectively (P < 0.05). DNA fragmentation ...
