The Experts below are selected from a list of 1482 Experts worldwide ranked by ideXlab platform
Seonjoo Park - One of the best experts on this subject based on the ideXlab platform.
-
Transcriptome-wide mining, characterization, and development of microsatellite markers in Lychnis kiusiana (Caryophyllaceae)
BMC plant biology, 2019Co-Authors: Seongjun Park, Sungwon Son, Myungju Shin, Noriyuki Fujii, Takuji Hoshino, Seonjoo ParkAbstract:Lychnis kiusiana Makino is an endangered perennial herb native to wetland areas in Korea and Japan. Despite its conservational and evolutionary significance, population genetic resources are lacking for this species. Next-generation sequencing has been accepted as a rapid and cost-effective solution for the identification of microsatellite markers in nonmodel plants. Using Illumina HiSeq 2000 sequencing technology, we assembled 67,498,600 reads into 91,900 contigs and identified 11,403 microsatellite repeat motifs in 9563 contigs. A total of 4510 microsatellite-containing transcripts had Gene Ontology (GO) annotations, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identified 124 pathways with significant scores. Many microsatellites in the L. kiusiana leaf transcriptome were linked to genes involved in the plant response to light intensity, salt stress, temperature stimulus, and nutrient and water deprivation. A total of 12,486 single-nucleotide polymorphisms (SNPs) were identified on transcripts harboring microsatellites. The analysis of nucleotide substitution rates for 2389 unigenes indicated that 39 genes were under strong positive selection. The primers of 6911 microsatellites were designed, and 40 of 50 selected primer pairs were consistently and successfully amplified from 51 individuals. Twenty-five of these were polymorphic, and the average number of alleles per SSR locus was 6.96, with a range from 2 to 15. The observed and expected heterozygosities ranged from 0.137 to 0.902 and 0.131 to 0.827, respectively, and locus-specific FIS estimates ranged from − 0.116 to 0.290. Eleven of the 25 primer pairs were successfully amplified in three additional species of Lychnis: 56% in L. wilfordii, 64% in L. cognata and 80% in L. fulgens. The transcriptomic SSR markers of Lychnis kiusiana provide a valuable resource for understanding the population genetics, evolutionary history, and effective conservation management of this species. Furthermore, the identified microsatellite loci linked to the annotated genes should be useful for developing functional markers of L. kiusiana. The developed markers represent a potentially valuable source of transcriptomic SSR markers for population genetic analyses with moderate levels of cross-taxon portability.
-
Additional file 1: of Transcriptome-wide mining, characterization, and development of microsatellite markers in Lychnis kiusiana (Caryophyllaceae)
2019Co-Authors: Seongjun Park, Sungwon Son, Myungju Shin, Noriyuki Fujii, Takuji Hoshino, Seonjoo ParkAbstract:Table S1. General information for the microsatellite analysis. Table S2. Distribution and characteristics of the microsatellites in different transcript regions. Table S3. Results of the KEGG pathway analysis. Table S4. Blast results of 9563 transcripts that contain SSRs in Lychnis leaf transcriptome. Table S5. Blast results of the 39 ORFs showing positive selection (dN/dSâ>â1). Table S6. Blast results of the 25 newly developed transcriptomic SSR markers. Table S7. NCBI accession numbers, primer sequences and characterization of the 25 microsatellite loci developed for Lychnis kiusiana. (DOCX 1498 kb
-
Additional file 2: of Transcriptome-wide mining, characterization, and development of microsatellite markers in Lychnis kiusiana (Caryophyllaceae)
2019Co-Authors: Seongjun Park, Sungwon Son, Myungju Shin, Noriyuki Fujii, Takuji Hoshino, Seonjoo ParkAbstract:Figure S1. The distributions of the major repeat types in the Lychnis kiusiana leaf transcriptome. Figure S2. Box plots of the sizes of different repeat motifs. Figure S3. Box plots of the sizes of different repeat motifs in different genic regions. Figure S4. Linkage disequilibrium (LD) map for the 25 transcriptomic SSR markers. (PDF 556 kb
-
Transcriptome-wide mining, characterization, and development of microsatellite markers in Lychnis kiusiana (Caryophyllaceae)
BMC, 2019Co-Authors: Seongjun Park, Sungwon Son, Myungju Shin, Noriyuki Fujii, Takuji Hoshino, Seonjoo ParkAbstract:Abstract Background Lychnis kiusiana Makino is an endangered perennial herb native to wetland areas in Korea and Japan. Despite its conservational and evolutionary significance, population genetic resources are lacking for this species. Next-generation sequencing has been accepted as a rapid and cost-effective solution for the identification of microsatellite markers in nonmodel plants. Results Using Illumina HiSeq 2000 sequencing technology, we assembled 67,498,600 reads into 91,900 contigs and identified 11,403 microsatellite repeat motifs in 9563 contigs. A total of 4510 microsatellite-containing transcripts had Gene Ontology (GO) annotations, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identified 124 pathways with significant scores. Many microsatellites in the L. kiusiana leaf transcriptome were linked to genes involved in the plant response to light intensity, salt stress, temperature stimulus, and nutrient and water deprivation. A total of 12,486 single-nucleotide polymorphisms (SNPs) were identified on transcripts harboring microsatellites. The analysis of nucleotide substitution rates for 2389 unigenes indicated that 39 genes were under strong positive selection. The primers of 6911 microsatellites were designed, and 40 of 50 selected primer pairs were consistently and successfully amplified from 51 individuals. Twenty-five of these were polymorphic, and the average number of alleles per SSR locus was 6.96, with a range from 2 to 15. The observed and expected heterozygosities ranged from 0.137 to 0.902 and 0.131 to 0.827, respectively, and locus-specific F IS estimates ranged from − 0.116 to 0.290. Eleven of the 25 primer pairs were successfully amplified in three additional species of Lychnis: 56% in L. wilfordii, 64% in L. cognata and 80% in L. fulgens. Conclusions The transcriptomic SSR markers of Lychnis kiusiana provide a valuable resource for understanding the population genetics, evolutionary history, and effective conservation management of this species. Furthermore, the identified microsatellite loci linked to the annotated genes should be useful for developing functional markers of L. kiusiana. The developed markers represent a potentially valuable source of transcriptomic SSR markers for population genetic analyses with moderate levels of cross-taxon portability
-
Comparative Genomics Perspective
2016Co-Authors: Gurusamy Raman, Seonjoo ParkAbstract:Dianthus superbus var. longicalycinus is an economically important traditional Chinese medicinal plant that is also used for ornamental purposes. In this study, D. superbus was compared to its closely related family of Caryophyllaceae chloroplast (cp) genomes such as Lychnis chalcedonica and Spinacia oleracea. D. superbus had the longest large single copy (LSC) region (82,805 bp), with some variations in the inverted repeat region A (IRA)/LSC regions. The IRs underwent both expansion and constriction during evolution of the Caryo-phyllaceae family; however, intense variations were not identified. The pseudogene ribo-somal protein subunit S19 (rps19) was identified at the IRA/LSC junction, but was not present in the cp genome of other Caryophyllaceae family members. The translation initia-tion factor IF-1 (infA) and ribosomal protein subunit L23 (rpl23) genes were absent from the Dianthus cp genome. When the cp genome of Dianthus was compared with 31 other angio-sperm lineages, the infA gene was found to have been lost in most members of rosids, sola-nales of asterids and Lychnis of Caryophyllales, whereas rpl23 gene loss or pseudogization had occurred exclusively in Caryophyllales. Nevertheless, the cp genome of Dianthus an
Abel Gizaw - One of the best experts on this subject based on the ideXlab platform.
-
colonization and diversification in the african sky islands insights from fossil calibrated molecular dating of Lychnis caryophyllaceae
New Phytologist, 2016Co-Authors: Abel Gizaw, Sileshi Nemomissa, Christian Brochmann, Tigist Wondimu, Catherine Aloyce Masao, Felly Mugizi TusiimeAbstract:The flora on the isolated high African mountains or 'sky islands' is remarkable for its peculiar adaptations, local endemism and striking biogeographical connections to remote parts of the world. Ages of the plant lineages and the timing of their radiations have frequently been debated but remain contentious as there are few estimates based on explicit models and fossil-calibrated molecular clocks. We used the plastid region maturaseK (matK) and a Caryophylloflora paleogenica fossil to infer the age of the genus Lychnis, and constructed a data set of three plastid (matK; a ribosomal protein S16 (rps16); and an intergenic spacer (psbE-petL)) and two nuclear (internal transcribed spacer (ITS) and a region spanning exon 18-24 in the second largest subunit of RNA polymerase II (RPB2)) loci for joint estimation of the species tree and divergence time of the African representatives. The time of divergence of the African high-altitude Lychnis was placed in the late Miocene to early Pliocene. A single speciation event was inferred in the early Pliocene; subsequent speciation took place sporadically from the late Pliocene to the middle Pleistocene. We provide further support for a Eurasian origin of the African 'sky islands' floral elements, which seem to have been recruited via dispersals at different times: some old, as in Lychnis, and others very recent. We show that dispersal and diversification within Africa play an important role in shaping these isolated plant communities.
-
Colonization and diversification in the African ‘sky islands’: insights from fossil‐calibrated molecular dating of Lychnis (Caryophyllaceae)
The New phytologist, 2016Co-Authors: Abel Gizaw, Magnus Popp, Bengt Oxelman, Sileshi Nemomissa, Christian Brochmann, Tigist Wondimu, Catherine Aloyce Masao, Felly Mugizi Tusiime, Ahmed Abdikadir Abdi, Dimitar DimitrovAbstract:The flora on the isolated high African mountains or 'sky islands' is remarkable for its peculiar adaptations, local endemism and striking biogeographical connections to remote parts of the world. Ages of the plant lineages and the timing of their radiations have frequently been debated but remain contentious as there are few estimates based on explicit models and fossil-calibrated molecular clocks. We used the plastid region maturaseK (matK) and a Caryophylloflora paleogenica fossil to infer the age of the genus Lychnis, and constructed a data set of three plastid (matK; a ribosomal protein S16 (rps16); and an intergenic spacer (psbE-petL)) and two nuclear (internal transcribed spacer (ITS) and a region spanning exon 18-24 in the second largest subunit of RNA polymerase II (RPB2)) loci for joint estimation of the species tree and divergence time of the African representatives. The time of divergence of the African high-altitude Lychnis was placed in the late Miocene to early Pliocene. A single speciation event was inferred in the early Pliocene; subsequent speciation took place sporadically from the late Pliocene to the middle Pleistocene. We provide further support for a Eurasian origin of the African 'sky islands' floral elements, which seem to have been recruited via dispersals at different times: some old, as in Lychnis, and others very recent. We show that dispersal and diversification within Africa play an important role in shaping these isolated plant communities.
-
ORIGINAL ARTICLE: Colonization and diversification in the African ‘sky islands’ by Eurasian Lychnis L. (Caryophyllaceae)
Journal of Biogeography, 2007Co-Authors: Magnus Popp, Abel Gizaw, Sileshi Nemomissa, Jan Suda, Christian BrochmannAbstract:Aim Many plants occurring on the isolated mountain peaks of Africa have their closest relatives in very remote areas, even in temperate Europe and Asia. Their biogeographical history is poorly understood. The Afro-montane element of the primarily Eurasian genus Lychnis is a typical example of such a disjunction. Here, we aim to reconstruct the phylogeny of the Afro-montane endemics of Lychnis and to infer the history of immigration into Africa and of the subsequent dispersal and speciation. Location The Afro-montane representatives of Lychnis occur in Ethiopia, Kenya, Tanzania, Uganda, Rwanda, Burundi, the Democratic Republic of Congo, Cameroon, and Nigeria. Methods Silica-dried plant materials collected in Ethiopia in 2004 as well as herbarium material were used for DNA extractions. We used parsimony analysis of nucleotide data from the rps16 intron and psbE-petL region in plastids, and the internal transcribed spacer (ITS) and a region spanning exon 18–24 in the second largest subunit of RNA polymerase II (RPB2) from the nucleus. DAPI-flow cytometry was used to determine relative genome size and to infer polyploidization events. Results Monophyly of the Afro-montane Lychnis with the Eurasian diploid L. flos-cuculi resolved as sister was strongly supported by the phylogenetic analyses. The Afro-montane group was further divided into two strongly supported groups correlated with DNA ploidy levels. The relative genome size was species-specific except for L. abyssinica, which had two genome-size variants. Main conclusions Our results demonstrate that the biogeographical history of the Afro-montane Lychnis has been highly dynamic, including polyploidization and both old and recent long-distance dispersal events, even between Eastern and West Africa. The ancestor is inferred to have immigrated once from Eurasia via the Arabian Peninsula to the Ethiopian highlands. Divergence in the lineage containing diploid taxa was followed by several dispersals from Ethiopia to the Eastern and Western Rift Mountains, and recently to West Africa. Divergence of the lineage including the tetraploid L. crassifolia may have taken place in Ethiopia, from where it dispersed to the Eastern and Western Rift Mountains; alternatively, it may have taken place in the Eastern Rift Mountains, followed by dispersal from there to the Western Rift Mountains and then to Ethiopia.
Seongjun Park - One of the best experts on this subject based on the ideXlab platform.
-
Transcriptome-wide mining, characterization, and development of microsatellite markers in Lychnis kiusiana (Caryophyllaceae)
BMC plant biology, 2019Co-Authors: Seongjun Park, Sungwon Son, Myungju Shin, Noriyuki Fujii, Takuji Hoshino, Seonjoo ParkAbstract:Lychnis kiusiana Makino is an endangered perennial herb native to wetland areas in Korea and Japan. Despite its conservational and evolutionary significance, population genetic resources are lacking for this species. Next-generation sequencing has been accepted as a rapid and cost-effective solution for the identification of microsatellite markers in nonmodel plants. Using Illumina HiSeq 2000 sequencing technology, we assembled 67,498,600 reads into 91,900 contigs and identified 11,403 microsatellite repeat motifs in 9563 contigs. A total of 4510 microsatellite-containing transcripts had Gene Ontology (GO) annotations, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identified 124 pathways with significant scores. Many microsatellites in the L. kiusiana leaf transcriptome were linked to genes involved in the plant response to light intensity, salt stress, temperature stimulus, and nutrient and water deprivation. A total of 12,486 single-nucleotide polymorphisms (SNPs) were identified on transcripts harboring microsatellites. The analysis of nucleotide substitution rates for 2389 unigenes indicated that 39 genes were under strong positive selection. The primers of 6911 microsatellites were designed, and 40 of 50 selected primer pairs were consistently and successfully amplified from 51 individuals. Twenty-five of these were polymorphic, and the average number of alleles per SSR locus was 6.96, with a range from 2 to 15. The observed and expected heterozygosities ranged from 0.137 to 0.902 and 0.131 to 0.827, respectively, and locus-specific FIS estimates ranged from − 0.116 to 0.290. Eleven of the 25 primer pairs were successfully amplified in three additional species of Lychnis: 56% in L. wilfordii, 64% in L. cognata and 80% in L. fulgens. The transcriptomic SSR markers of Lychnis kiusiana provide a valuable resource for understanding the population genetics, evolutionary history, and effective conservation management of this species. Furthermore, the identified microsatellite loci linked to the annotated genes should be useful for developing functional markers of L. kiusiana. The developed markers represent a potentially valuable source of transcriptomic SSR markers for population genetic analyses with moderate levels of cross-taxon portability.
-
Additional file 1: of Transcriptome-wide mining, characterization, and development of microsatellite markers in Lychnis kiusiana (Caryophyllaceae)
2019Co-Authors: Seongjun Park, Sungwon Son, Myungju Shin, Noriyuki Fujii, Takuji Hoshino, Seonjoo ParkAbstract:Table S1. General information for the microsatellite analysis. Table S2. Distribution and characteristics of the microsatellites in different transcript regions. Table S3. Results of the KEGG pathway analysis. Table S4. Blast results of 9563 transcripts that contain SSRs in Lychnis leaf transcriptome. Table S5. Blast results of the 39 ORFs showing positive selection (dN/dSâ>â1). Table S6. Blast results of the 25 newly developed transcriptomic SSR markers. Table S7. NCBI accession numbers, primer sequences and characterization of the 25 microsatellite loci developed for Lychnis kiusiana. (DOCX 1498 kb
-
Additional file 2: of Transcriptome-wide mining, characterization, and development of microsatellite markers in Lychnis kiusiana (Caryophyllaceae)
2019Co-Authors: Seongjun Park, Sungwon Son, Myungju Shin, Noriyuki Fujii, Takuji Hoshino, Seonjoo ParkAbstract:Figure S1. The distributions of the major repeat types in the Lychnis kiusiana leaf transcriptome. Figure S2. Box plots of the sizes of different repeat motifs. Figure S3. Box plots of the sizes of different repeat motifs in different genic regions. Figure S4. Linkage disequilibrium (LD) map for the 25 transcriptomic SSR markers. (PDF 556 kb
-
Transcriptome-wide mining, characterization, and development of microsatellite markers in Lychnis kiusiana (Caryophyllaceae)
BMC, 2019Co-Authors: Seongjun Park, Sungwon Son, Myungju Shin, Noriyuki Fujii, Takuji Hoshino, Seonjoo ParkAbstract:Abstract Background Lychnis kiusiana Makino is an endangered perennial herb native to wetland areas in Korea and Japan. Despite its conservational and evolutionary significance, population genetic resources are lacking for this species. Next-generation sequencing has been accepted as a rapid and cost-effective solution for the identification of microsatellite markers in nonmodel plants. Results Using Illumina HiSeq 2000 sequencing technology, we assembled 67,498,600 reads into 91,900 contigs and identified 11,403 microsatellite repeat motifs in 9563 contigs. A total of 4510 microsatellite-containing transcripts had Gene Ontology (GO) annotations, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identified 124 pathways with significant scores. Many microsatellites in the L. kiusiana leaf transcriptome were linked to genes involved in the plant response to light intensity, salt stress, temperature stimulus, and nutrient and water deprivation. A total of 12,486 single-nucleotide polymorphisms (SNPs) were identified on transcripts harboring microsatellites. The analysis of nucleotide substitution rates for 2389 unigenes indicated that 39 genes were under strong positive selection. The primers of 6911 microsatellites were designed, and 40 of 50 selected primer pairs were consistently and successfully amplified from 51 individuals. Twenty-five of these were polymorphic, and the average number of alleles per SSR locus was 6.96, with a range from 2 to 15. The observed and expected heterozygosities ranged from 0.137 to 0.902 and 0.131 to 0.827, respectively, and locus-specific F IS estimates ranged from − 0.116 to 0.290. Eleven of the 25 primer pairs were successfully amplified in three additional species of Lychnis: 56% in L. wilfordii, 64% in L. cognata and 80% in L. fulgens. Conclusions The transcriptomic SSR markers of Lychnis kiusiana provide a valuable resource for understanding the population genetics, evolutionary history, and effective conservation management of this species. Furthermore, the identified microsatellite loci linked to the annotated genes should be useful for developing functional markers of L. kiusiana. The developed markers represent a potentially valuable source of transcriptomic SSR markers for population genetic analyses with moderate levels of cross-taxon portability
Catherine Aloyce Masao - One of the best experts on this subject based on the ideXlab platform.
-
colonization and diversification in the african sky islands insights from fossil calibrated molecular dating of Lychnis caryophyllaceae
New Phytologist, 2016Co-Authors: Abel Gizaw, Sileshi Nemomissa, Christian Brochmann, Tigist Wondimu, Catherine Aloyce Masao, Felly Mugizi TusiimeAbstract:The flora on the isolated high African mountains or 'sky islands' is remarkable for its peculiar adaptations, local endemism and striking biogeographical connections to remote parts of the world. Ages of the plant lineages and the timing of their radiations have frequently been debated but remain contentious as there are few estimates based on explicit models and fossil-calibrated molecular clocks. We used the plastid region maturaseK (matK) and a Caryophylloflora paleogenica fossil to infer the age of the genus Lychnis, and constructed a data set of three plastid (matK; a ribosomal protein S16 (rps16); and an intergenic spacer (psbE-petL)) and two nuclear (internal transcribed spacer (ITS) and a region spanning exon 18-24 in the second largest subunit of RNA polymerase II (RPB2)) loci for joint estimation of the species tree and divergence time of the African representatives. The time of divergence of the African high-altitude Lychnis was placed in the late Miocene to early Pliocene. A single speciation event was inferred in the early Pliocene; subsequent speciation took place sporadically from the late Pliocene to the middle Pleistocene. We provide further support for a Eurasian origin of the African 'sky islands' floral elements, which seem to have been recruited via dispersals at different times: some old, as in Lychnis, and others very recent. We show that dispersal and diversification within Africa play an important role in shaping these isolated plant communities.
-
Colonization and diversification in the African ‘sky islands’: insights from fossil‐calibrated molecular dating of Lychnis (Caryophyllaceae)
The New phytologist, 2016Co-Authors: Abel Gizaw, Magnus Popp, Bengt Oxelman, Sileshi Nemomissa, Christian Brochmann, Tigist Wondimu, Catherine Aloyce Masao, Felly Mugizi Tusiime, Ahmed Abdikadir Abdi, Dimitar DimitrovAbstract:The flora on the isolated high African mountains or 'sky islands' is remarkable for its peculiar adaptations, local endemism and striking biogeographical connections to remote parts of the world. Ages of the plant lineages and the timing of their radiations have frequently been debated but remain contentious as there are few estimates based on explicit models and fossil-calibrated molecular clocks. We used the plastid region maturaseK (matK) and a Caryophylloflora paleogenica fossil to infer the age of the genus Lychnis, and constructed a data set of three plastid (matK; a ribosomal protein S16 (rps16); and an intergenic spacer (psbE-petL)) and two nuclear (internal transcribed spacer (ITS) and a region spanning exon 18-24 in the second largest subunit of RNA polymerase II (RPB2)) loci for joint estimation of the species tree and divergence time of the African representatives. The time of divergence of the African high-altitude Lychnis was placed in the late Miocene to early Pliocene. A single speciation event was inferred in the early Pliocene; subsequent speciation took place sporadically from the late Pliocene to the middle Pleistocene. We provide further support for a Eurasian origin of the African 'sky islands' floral elements, which seem to have been recruited via dispersals at different times: some old, as in Lychnis, and others very recent. We show that dispersal and diversification within Africa play an important role in shaping these isolated plant communities.
Barbara Thiem - One of the best experts on this subject based on the ideXlab platform.
-
Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?
Plant Cell Tissue and Organ Culture (PCTOC), 2019Co-Authors: Michał P. Maliński, Małgorzata Kikowska, Dariusz Kruszka, Marta Napierała, Ewa Florek, Elwira Sliwinska, Barbara ThiemAbstract:Diverse types of Lychnis flos-cuculi in vitro cultures have been developed. HPLC analysis demonstrated much higher ecdysteroid content in cultures and regenerated plants when compared to natural site plants. Lychnis flos - cuculi L. is a species containing ecdysteroids, triterpenoid saponins, flavonoids, and phenolic acids, and therefore is a plant of potential medicinal value. In the presented research, diverse in vitro cultures of this taxon were developed to obtain the uniform material capable of producing ecdysteroids, including micropropagated plantlets, shoot cultures, liquid agitated whole plant cultures with fast-growing roots, and callus. A protocol of micropropagation through axillary bud formation was established using plant growth regulators at different concentrations and combinations. All the variants of plant growth regulator supplementation significantly affected a shoot proliferation rate ranging from 8 to 16 plantlets per explant, depending on the medium; DNA content of all the studied plant materials was similar. The thin-layer chromatography analysis of the extracts revealed the presence of ecdysteroids in every plant material apart from callus. The content of 20-hydroxyecdysone and polypodine B was evaluated by high-performance liquid chromatography. Agitated plantlets were in vitro cultures that efficiently formed abundant root biomass with significant concentrations of ecdysteroids. In vitro-derived adventitious roots contained twofold higher content of ecdysteroids than those of intact plants. The organs of flowering in vitro-propagated plants, transferred to experimental plot, contained twice as much ecdysteroids when compared to the organs of plants from the natural site, among which flowers were the richest in ecdysteroids. The results revealed that adventitious roots from L. flos - cuculi agitated cultures can be considered as an alternative biotechnological source of biomass rich in pharmaceutically active ecdysteroids.
-
Ragged Robin (Lychnis flos-cuculi) - a plant with potential medicinal value
Elsevier, 2014Co-Authors: Michał P. Maliński, Anna D. Michalska, Monika Tomczykowa, Michał Tomczyk, Barbara ThiemAbstract:Lychnis flos-cuculi L., Caryophyllaceae, contains a number of active compounds belonging to several chemical groups. Previous studies have led to the identification of phytoecdysteroids, triterpenoids saponins, volatile compounds, fatty acid derivatives, phenolic acids and flavonoids. Research on pharmacological activity showed that plant extracts inhibited the growth of bacteria and fungi. The antimitotic properties of preparations from the herb L. flos-cuculi were also reported. The phytochemical analyses demonstrated that this taxon contains pharmaceutically promising compounds, but more phytochemical and pharmacological studies of L. flos-cuculi are needed for further information regarding this plant. This review summarizes reports regarding chemical composition and biological activity of L. flos-cuculi as well as several cognate species, which pose opportunities related to in vitro propagation and cell and tissue cultures. In vitro-regen-erated plantlets could be a good source of genetically uniform plant material for future research. Keywords: Lychnis flos-cuculi, Taxonomy, Secondary metabolites, Biological activity, In vitro cultures, Micropropagatio
