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Yacob Garedaghi - One of the best experts on this subject based on the ideXlab platform.
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prevalence of linguatula serrata nymph in goat in tabriz north west of iran
Veterinary Research Forum, 2011Co-Authors: Yacob GaredaghiAbstract:Linguatula serrata is one of well-known members of Pentastomida which infects both humans and animals. The aim of this study was to evaluate the prevalence of L. serrata in mesenteric Lymph nodes, livers and lungs of goats slaughtered in Tabriz area, Iran. Mesenteric Lymph nodes (MLNs), livers and lungs of 280 goats slaughtered in Tabriz, North-west of Iran were investigated for nymphs of L. serrata from September 2009 to September 2010. The organs were examined macroscopically and then a tissue digestion method was also done for investigation of liver and lung of the goats that were infected MLN. The liver and lung samples were mostly taken from the condemned and rejected part of organs. The infection rate of L. serrata nymphs in MLNs, livers and lungs was 27.1 %, 2.8 % and 2.8 % respectively. The number of isolated nymph in infected Lymph nodes varied from 1 to 22 with a mean of 7. Only one nymph was isolated from each infected livers and lungs. The infection rate increased with age (P 0.05). Linguatula infection occurs as an endemic zoonosis in the study area and has an active transmission life cycle.
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Prevalence of Linguatula serrata Nymph in Goat in Tabriz, North-West of Iran
Urmia University, 2011Co-Authors: Yacob GaredaghiAbstract:Linguatula serrata is one of well-known members of Pentastomida which infects both humans and animals. The aim of this study was to evaluate the prevalence of L. serrata in mesenteric Lymph nodes, livers and lungs of goats slaughtered in Tabriz area, Iran. Mesenteric Lymph nodes (MLNs), livers and lungs of 280 goats slaughtered in Tabriz, North-west of Iran were investigated for nymphs of L. serrata from September 2009 to September 2010. The organs were examined macroscopically and then a tissue digestion method was also done for investigation of liver and lung of the goats that were infected MLN. The liver and lung samples were mostly taken from the condemned and rejected part of organs. The infection rate of L. serrata nymphs in MLNs, livers and lungs was 27.1 %, 2.8 % and 2.8 % respectively. The number of isolated nymph in infected Lymph nodes varied from 1 to 22 with a mean of 7. Only one nymph was isolated from each infected livers and lungs. The infection rate increased with age (P < 0.05). No significant difference in different sex groups and seasons was observed (P > 0.05). Linguatula infection occurs as an endemic zoonosis in the study area and has an active transmission life cycle
Patrice Mathevet - One of the best experts on this subject based on the ideXlab platform.
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clinical significance of Lympho vascular space involvement and Lymph node micrometastases in early stage cervical cancer a retrospective case control surgico pathological study
Gynecologic Oncology, 2005Co-Authors: Pierangelo Marchiolè, Annie Buénerd, Daniel Dargent, Mehdi Benchaib, Karima Nezhat, Patrice MathevetAbstract:Abstract Objective Several studies have shown that Lympho vascular space involvement (LVSI) and Lymph node micrometastases (LNmM) may be risk factors for recurrence in early-stage cervical cancer with no apparent Lymph node metastases. We performed a retrospective case-control study to reassess whether the presence of Lymph node micrometastases and LVSI is predictive of subsequent recurrence following surgical resection of early-stage cervical cancer. Methods In a series of 292 patients diagnosed with early cervical cancer and treated by the same surgical procedure (laparoscopic-vaginal radical hysterectomy) during the same time period, two paired series were selected. The first series consisted of 26 cases who recurred in a median time of 36.8 months and the second series were 26 cases matched for age, histological sub-type, surgico-pathological stage and maximal tumor diameter, who did not recur after a median follow-up of 122 months. Sections taken from the hysterectomy specimens were reassessed for LVSI. All the Lymph node blocks which have initially been considered as uninvolved were submitted to serial sectioning. Immunohistochemical staining using anti-cytokeratins AE1 and AE3 was used for identifying LNmM. Results LVSI was twice more frequent and LNmM ten-fold more frequent in the group of patients who recurred: 20/26 (77%) versus 9/26 (35%) and 11/26 (42%) versus 1/26 (4%) respectively. The relative risk of recurrence is 2.64 (1.67–5.49, P P Conclusions LNmM is an important risk factor of tumor recurrence in patients with early cervical cancer with no apparent Lymph node metastases. LNmM seems to occur only in LVSI positive tumors. These data may lead to improve management of early-stage cervical cancer to reduce the risk of recurrence in those cases.
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Sentinel Lymph node biopsy is not accurate in predicting Lymph node status for patients with cervical carcinoma.
Cancer, 2004Co-Authors: Pierangelo Marchiolè, Annie Buénerd, Daniel Dargent, Jean-yves Scoazec, Patrice MathevetAbstract:BACKGROUND The authors evaluated the accuracy of sentinel Lymph node biopsy in predicting Lymph node status for patients with early cervical carcinoma. In particular, the authors set out to determine the false-negative rate associated with sentinel Lymph node biopsy in this setting. METHODS Twenty-nine consecutive patients with early cervical carcinoma who were treated with pelvic laparoscopic Lymphadenectomy and radical surgery underwent sentinel Lymph node biopsy following Lymphatic mapping with patent blue dye. All sentinel and nonsentinel Lymph nodes were evaluated for micrometastases via multilevel sectioning followed by immunohistochemical staining. RESULTS At least one sentinel Lymph node was identified for each patient. On routine pathologic evaluation, 3 patients (10%) were found to have positive Lymph nodes. Among the remaining 26 patients, multilevel sectioning in conjunction with immunohistochemical analysis identified 5 patients (19%) who had micrometastases in the pelvic Lymph nodes. Two of these five patients had micrometastases in a sentinel Lymph node; however, the more notable finding was that the other three patients had micrometastases in nonsentinel pelvic Lymph nodes despite having negative findings on sentinel Lymph node biopsy. Thus, the negative predictive value of sentinel Lymph node biopsy in the current study was 87.5%. CONCLUSIONS Multilevel sectioning followed by cytokeratin immunohistochemistry may identify additional patients who have Lymph node micrometastases; in the current study, this technique identified cases in which micrometastases were present in nonsentinel Lymph nodes even when sentinel Lymph nodes were found to be negative for disease on biopsy. This high false-negative rate associated with sentinel Lymph node biopsy, raises questions regarding the validity of the sentinel Lymph node concept in cervical carcinoma. Cancer 2004. © 2004 American Cancer Society.
A. Frick - One of the best experts on this subject based on the ideXlab platform.
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Experimental study on the microsurgical or spontaneous formation of Lympho-Lymphonodular anastomoses in the rat model
Journal of plastic reconstructive & aesthetic surgery : JPRAS, 2011Co-Authors: J. Wallmichrath, Ruediger G. H. Baumeister, Tanja Herrler, Axel Greiner, Oliver Pieske, Riccardo E. Giunta, A. FrickAbstract:Summary Background The restoration of the Lymphatic function in chronic Lymphoedemas can be achieved by microsurgical Lymphatic vessel transplantation. Usually, end-to-end anastomoses are performed between the Lymphatic vessels. Sometimes, the lack of appropriate Lymphatic recipient vessels impels the idea of connecting the grafts to local Lymph nodes. This study focusses on the microsurgical completion of durable Lympho-Lymphonodular anastomoses and further the detection of a spontaneous formation. Methods In 36 Sprague-Dawley rats the retroperitoneal Lymphatic structures were prepared after staining with patent blue V dye. In group A ( n = 12), the left lumbar trunk was cut cranially and its distal part was turned over to the right lumbar Lymph node where a microsurgical Lympho-Lymphonodular anastomosis was performed. In group B ( n = 12), treatment was similar but without anastomosing. In group C ( n = 12), the left lumbar trunk was transected and the capsule of the contralateral lumbar Lymph node was incised. Finally, the lumbar region was re-exposed and the Lymphatic drainage was examined using patent blue in four animals of each group after 8, 12 and 16 weeks, respectively, to examine the development of possible spontaneous formations of Lymphatic connections. Results In 12/12 animals of group A, patent transposed Lymph vessels and anastomoses with blue staining of the right lumbar Lymph node were observed. Only 1/12 animals of group B and 2/12 animals of group C showed a blue staining of the right lumbar Lymph node. Conclusions The microsurgical fabrication of Lympho-Lymphonodular anastomoses leads to a safe and durable Lymphatic connection. The known plasticity of Lymphatic vessels with the potential of spontaneous anastomosing seems to be higher between Lymphatic vessels than between Lymphatic vessels and a Lymph node.
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Technique and proof of patency of microsurgical Lympho-Lymphonodular anastomoses: a study in the rat model.
Microsurgery, 2009Co-Authors: J. Wallmichrath, Ruediger G. H. Baumeister, Axel Greiner, Claus J. Deglmann, Sibylle Heim, A. FrickAbstract:Background: Lymphedemas due to local Lymphatic blocks can be treated by microsurgical transplantation or transposition of Lymphatic vessels. Here, the anastomoses are usually made end-to-end between Lymphatics, but occasionally appropriate Lymphatic recipient vessels are missing. In such cases, reconstructing Lymph drainage by connection to a Lymph node could be another technical option. The purpose of this study was to examine the patency rate of such Lympho-Lymphonodular anastomoses in an experimental animal model. Methods: Male Sprague–Dawley rats were anesthetized, and the retroperitoneum was exposed. Patent blue dye was injected into the left foot to stain Lymphatic structures. In group A (n = 8), the left lumbar trunk was cut centrally, the distal part was turned over to the right lumbar Lymph node, and a microsurgical Lympho-Lymphonodular anastomosis was performed. In group B (n = 8), the left lumbar trunk was cut. After 8 weeks, the lumbar region was surgically re-explored, and the Lymphatic drainage was examined by injection of Patent blue dye into the left lumbar Lymph node. Results: In 8/8 animals of group A, patent transposed Lymphatics were found. The patency of the anastomosis was proven directly by observation of blue dye transit and indirectly by observation of blue staining of the right lumbar Lymph node. In 6/8 animals of group B, no Lymphatic connection to the right lumbar Lymphatic system was observed. Conclusions: This is the first report of the microsurgical technique and the proof of patency of Lympho-Lymphonodular anastomoses. The novel animal model for testing the patency of transposed Lymphatics is discussed. © 2009 Wiley-Liss, Inc. Microsurgery, 2009.
Masayuki Imamura - One of the best experts on this subject based on the ideXlab platform.
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A strategy for determining which thoracic esophageal cancer patients should undergo cervical Lymph node dissection.
The Annals of Thoracic Surgery, 2005Co-Authors: Shiro Nagatani, Junichi Kaganoi, Masato Kondo, Masato Maeda, Yutaka Shimada, Go Watanabe, Masayuki ImamuraAbstract:Background There is controversy about performing cervical Lymph node dissections in all middle and lower thoracic esophageal squamous cell carcinoma patients. The purpose of this study was to evaluate whether intraoperative examination of thoracic paratracheal Lymph node by real-time reverse transcription-polymerase chain reaction was worthwhile for selecting patients for cervical Lymph node dissection. Methods Under informed consent, 30 middle and lower thoracic esophageal squamous cell carcinoma patients were examined for thoracic paratracheal Lymph node metastasis intraoperatively by hematoxylin-eosin staining and real-time reverse transcription-polymerase chain reaction for messenger RNA encoding squamous cell carcinoma antigen. When thoracic paratracheal Lymph node metastasis was found, cervical Lymph node dissection was performed. If the patients had no thoracic paratracheal Lymph node metastasis, a randomized study for selection of cervical Lymph node dissection was performed. Results Eleven of 30 patients with middle or lower third thoracic esophageal squamous cell carcinoma had thoracic paratracheal Lymph node metastasis. Five of these 11 patients had cervical Lymph node metastasis. Nineteen patients who had no metastasis in the thoracic paratracheal Lymph nodes were enrolled in a randomized study. Eight of the 19 patients received cervical Lymph node dissection and they were found not to have cervical Lymph node metastasis. The other 11 patients did not receive cervical Lymph node dissection, and there was no cervical Lymph node recurrence. Conclusions The intraoperative diagnosis of metastasis in the thoracic paratracheal Lymph node may be used as an indicator for cervical Lymph node dissection in middle and lower thoracic esophageal cancer patients.
Steven J. Mentzer - One of the best experts on this subject based on the ideXlab platform.
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Biochemical changes in the efferent Lymph plasma after oxazolone stimulation
Developmental and comparative immunology, 2002Co-Authors: Charles A. West, George A. Fischer, Alan J. Young, Steven J. MentzerAbstract:The Lymph node is a specialized microenvironment for the regulation of immune responses. The reticular network of the Lymph node provides a structure that facilitates not only intercellular interactions, but the intranodal flow of Lymph fluid. To assess biochemical changes in the nodal Lymph plasma after antigen stimulation, prescapular Lymph nodes in sheep were stimulated with the epicutaneous antigen oxazolone. The efferent Lymph from both antigen-stimulated and contralateral control prescapular Lymph nodes was continuously monitored for more than 120 h. The oxazolone-stimulated Lymph plasma was associated with a selective increase in cholesterol content during the 'recruitment' phase of Lymph node enlargement. The peak in cholesterol was followed closely by a significant increase in Lymph plasma LDH concentration. In contrast, there was no significant difference between oxazolone-stimulated and control Lymph plasma in the concentration of triglycerides, albumin, alkaline phosphatase, and alanine transferase. These selective biochemical changes in the efferent Lymph appeared to reflect the dynamics of Lymphocyte activation within the Lymph node as well as provide a practical measure of the Lymph node response to antigen stimulation.
