The Experts below are selected from a list of 21 Experts worldwide ranked by ideXlab platform
Matti Kaartinen - One of the best experts on this subject based on the ideXlab platform.
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two novel human b cell lymphoma lines of Lymphatic Follicle origin cytogenetic molecular genetic and histopathological characterisation
European Journal of Haematology, 2009Co-Authors: Sakari Knuutila, Mine Eray, Juha Klefstrom, Jadwiga Szymanska, Taina Lakkala, Paivi Peltomaki, Lasse Teerenhovi, Erkki Elonen, Kaarle Franssila, Matti KaartinenAbstract:: Two B-cell lines, designated as HF-1 and HF-4, were characterised. The cell lines have complicated karyotype abnormalities including a 14;18 translocation and an 8q24 breakpoint originating from t(2;8)(p11;q24) (HF-1) or t(1;8)(p21:q24) (HF-4). The lines have BCL2 rearrangement and they are positive for CD19, CD20, CD22, CD39. HF-1 is also positive for IgG, and HF-4 is positive for IgM and IgD. On Northern blot analyses, the 2.6-kb and 4.2-kb transcripts corresponding to the major transcripts of CMYC and BCL2, respectively, were seen. In Western blot as well as in FACS (fluorescence-activated cell sorting) analysis the presence of BCL2 protein in the both HF-1 and HF-4 cells was demonstrated. The cell lines are expected to serve as an important tool in the study of the chromosomal mechanism activating cellular oncogenes, the somatic hypermutation mechanism of antigen-activated B cells and the apoptosis of B cells.
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somatic hypermutations in the immunoglobulin genes of two new human lymphoma lines of Lymphatic Follicle origin
Scandinavian Journal of Immunology, 1994Co-Authors: T Tuomikoski, Mine Eray, Petri S Mattila, Sakari Knuutila, Matti KaartinenAbstract:Variable immunoglobulin heavy-chain regions (VDJ) of two newly established human lymphoma cell lines (HF-1 and HF-4) were sequenced. The most homologous germline VH gene found for both the HF-1 and HF-4 sequences was VH26 of the VH3a (V gene) family (82% and 91 % homologies, respectively). The JH region of the HF-4 heavy-chain sequence contained two nucleotide differences compared to the published germline JH3 gene. The DHJH region of the HF-1 gene had a record high number (20%) of somatic mutations. The numerous hypermutations found in the HF-1 cell line support the hypothesis that in some human follicular lymphomas, mutations continue to accumulate in immunoglobulin genes during the malignant growth. Follicular lymphoma cell lines, which have an active mutational machinery, in future may help to solve the molecular events behind the somatic hypermutations modifying immunoglobulin genes of B lymphocytes.
Vaclav Hořejsi - One of the best experts on this subject based on the ideXlab platform.
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expression pattern of adaptor protein pag correlation between secondary Lymphatic Follicle and histogenetically related malignant lymphomas
Immunology Letters, 2005Co-Authors: Alexandr Svec, Zuzana Velenska, Vaclav HořejsiAbstract:Transmembrane adaptor protein PAG, also known as Csk-binding protein (Cbp), which binds and activates the cytoplasmic tyrosine kinase Csk, the major negative regulator of Src-family kinases, was found to be expressed in germinal centers of lymphoid Follicles as well as in follicular, but not mantle cell lymphomas. Expression of PAG may reflect its role in regulation of proliferation and differentiation of germinal center B-cells. From the routine histopathology point of view, PAG might be a new positive marker of follicular lymphoma and a negative marker of mantle cell lymphoma.
Sakari Knuutila - One of the best experts on this subject based on the ideXlab platform.
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two novel human b cell lymphoma lines of Lymphatic Follicle origin cytogenetic molecular genetic and histopathological characterisation
European Journal of Haematology, 2009Co-Authors: Sakari Knuutila, Mine Eray, Juha Klefstrom, Jadwiga Szymanska, Taina Lakkala, Paivi Peltomaki, Lasse Teerenhovi, Erkki Elonen, Kaarle Franssila, Matti KaartinenAbstract:: Two B-cell lines, designated as HF-1 and HF-4, were characterised. The cell lines have complicated karyotype abnormalities including a 14;18 translocation and an 8q24 breakpoint originating from t(2;8)(p11;q24) (HF-1) or t(1;8)(p21:q24) (HF-4). The lines have BCL2 rearrangement and they are positive for CD19, CD20, CD22, CD39. HF-1 is also positive for IgG, and HF-4 is positive for IgM and IgD. On Northern blot analyses, the 2.6-kb and 4.2-kb transcripts corresponding to the major transcripts of CMYC and BCL2, respectively, were seen. In Western blot as well as in FACS (fluorescence-activated cell sorting) analysis the presence of BCL2 protein in the both HF-1 and HF-4 cells was demonstrated. The cell lines are expected to serve as an important tool in the study of the chromosomal mechanism activating cellular oncogenes, the somatic hypermutation mechanism of antigen-activated B cells and the apoptosis of B cells.
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somatic hypermutations in the immunoglobulin genes of two new human lymphoma lines of Lymphatic Follicle origin
Scandinavian Journal of Immunology, 1994Co-Authors: T Tuomikoski, Mine Eray, Petri S Mattila, Sakari Knuutila, Matti KaartinenAbstract:Variable immunoglobulin heavy-chain regions (VDJ) of two newly established human lymphoma cell lines (HF-1 and HF-4) were sequenced. The most homologous germline VH gene found for both the HF-1 and HF-4 sequences was VH26 of the VH3a (V gene) family (82% and 91 % homologies, respectively). The JH region of the HF-4 heavy-chain sequence contained two nucleotide differences compared to the published germline JH3 gene. The DHJH region of the HF-1 gene had a record high number (20%) of somatic mutations. The numerous hypermutations found in the HF-1 cell line support the hypothesis that in some human follicular lymphomas, mutations continue to accumulate in immunoglobulin genes during the malignant growth. Follicular lymphoma cell lines, which have an active mutational machinery, in future may help to solve the molecular events behind the somatic hypermutations modifying immunoglobulin genes of B lymphocytes.
Alexandr Svec - One of the best experts on this subject based on the ideXlab platform.
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expression pattern of adaptor protein pag correlation between secondary Lymphatic Follicle and histogenetically related malignant lymphomas
Immunology Letters, 2005Co-Authors: Alexandr Svec, Zuzana Velenska, Vaclav HořejsiAbstract:Transmembrane adaptor protein PAG, also known as Csk-binding protein (Cbp), which binds and activates the cytoplasmic tyrosine kinase Csk, the major negative regulator of Src-family kinases, was found to be expressed in germinal centers of lymphoid Follicles as well as in follicular, but not mantle cell lymphomas. Expression of PAG may reflect its role in regulation of proliferation and differentiation of germinal center B-cells. From the routine histopathology point of view, PAG might be a new positive marker of follicular lymphoma and a negative marker of mantle cell lymphoma.
Mine Eray - One of the best experts on this subject based on the ideXlab platform.
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two novel human b cell lymphoma lines of Lymphatic Follicle origin cytogenetic molecular genetic and histopathological characterisation
European Journal of Haematology, 2009Co-Authors: Sakari Knuutila, Mine Eray, Juha Klefstrom, Jadwiga Szymanska, Taina Lakkala, Paivi Peltomaki, Lasse Teerenhovi, Erkki Elonen, Kaarle Franssila, Matti KaartinenAbstract:: Two B-cell lines, designated as HF-1 and HF-4, were characterised. The cell lines have complicated karyotype abnormalities including a 14;18 translocation and an 8q24 breakpoint originating from t(2;8)(p11;q24) (HF-1) or t(1;8)(p21:q24) (HF-4). The lines have BCL2 rearrangement and they are positive for CD19, CD20, CD22, CD39. HF-1 is also positive for IgG, and HF-4 is positive for IgM and IgD. On Northern blot analyses, the 2.6-kb and 4.2-kb transcripts corresponding to the major transcripts of CMYC and BCL2, respectively, were seen. In Western blot as well as in FACS (fluorescence-activated cell sorting) analysis the presence of BCL2 protein in the both HF-1 and HF-4 cells was demonstrated. The cell lines are expected to serve as an important tool in the study of the chromosomal mechanism activating cellular oncogenes, the somatic hypermutation mechanism of antigen-activated B cells and the apoptosis of B cells.
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somatic hypermutations in the immunoglobulin genes of two new human lymphoma lines of Lymphatic Follicle origin
Scandinavian Journal of Immunology, 1994Co-Authors: T Tuomikoski, Mine Eray, Petri S Mattila, Sakari Knuutila, Matti KaartinenAbstract:Variable immunoglobulin heavy-chain regions (VDJ) of two newly established human lymphoma cell lines (HF-1 and HF-4) were sequenced. The most homologous germline VH gene found for both the HF-1 and HF-4 sequences was VH26 of the VH3a (V gene) family (82% and 91 % homologies, respectively). The JH region of the HF-4 heavy-chain sequence contained two nucleotide differences compared to the published germline JH3 gene. The DHJH region of the HF-1 gene had a record high number (20%) of somatic mutations. The numerous hypermutations found in the HF-1 cell line support the hypothesis that in some human follicular lymphomas, mutations continue to accumulate in immunoglobulin genes during the malignant growth. Follicular lymphoma cell lines, which have an active mutational machinery, in future may help to solve the molecular events behind the somatic hypermutations modifying immunoglobulin genes of B lymphocytes.
