The Experts below are selected from a list of 3405 Experts worldwide ranked by ideXlab platform
Francis A Ennis - One of the best experts on this subject based on the ideXlab platform.
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a cd4 cytotoxic t Lymphocyte Clone to a conserved epitope on human immunodeficiency virus type 1 p24 cytotoxic activity and secretion of interleukin 2 and interleukin 6
Journal of Virology, 1992Co-Authors: Rebecca A Littaua, Michael B A Oldstone, Akira Takeda, Francis A EnnisAbstract:A CD4+ cytotoxic T-Lymphocyte (CTL) Clone, established from the peripheral blood of a human immunodeficiency virus (HIV)-seropositive donor, lysed autologous target cells that were infected with a recombinant vaccinia virus containing the gag gene of HIV type 1 and target cells pulsed with p24gag construct expressed in Escherichia coli. The recognition of the HLA-DQ-restricted epitope by this Clone was further defined by using overlapping synthetic peptides. The epitope recognized by this CD4+ CTL Clone (amino acids 140 to 148) overlaps with a CD8+ epitope and is highly conserved among all isolates of HIV type 1 that have been sequenced. Production and secretion of lymphokines such as interleukin-2 and interleukin-6 after specific antigenic stimulation were demonstrated by this gag-specific CD4+ CTL Clone.
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specific lysis of human immunodeficiency virus type 1 infected cells by a hla a3 1 restricted cd8 cytotoxic t Lymphocyte Clone that recognizes a conserved peptide sequence within the gp41 subunit of the envelope protein
Proceedings of the National Academy of Sciences of the United States of America, 1991Co-Authors: Kazuo Takahashi, Bernard Moss, Li Chen Dai, Thomas R Fuerst, William E Biddison, Patricia L Earl, Francis A EnnisAbstract:Abstract A HLA-A3.1-restricted CD8+ cytotoxic T-cell Clone, E7.20, that lyses cells infected with human immunodeficiency virus type 1 was isolated from an infected individual. The epitope was localized to amino acids 768-778 (RLRDLLLIVTR, NL43 env sequence) of the cytoplasmic domain of gp41 by successive use of a panel of recombinant vaccinia viruses that express truncated env genes and synthetic peptides. The epitope is conserved on 7 (NL43, BRU, HXB2, BRVA, SC, JH3, and JFL) of 13 human immunodeficiency virus type 1 isolates from North America. Synthetic peptides of this region of strains RF and CDC4 are also recognized by E7.20 despite a nonconservative Thr----Val or Thr----Ala change at amino acid 777; however, an MN peptide, which has four amino acid substitutions, was not reactive. The epitope recognized by E7.20 has a predicted hydrophobic alpha-helical structure, with three contiguous Leu residues followed by Ile and Val at amino acids 772-776. Cytotoxicity was restricted by HLA-A3.1 using allogeneic target cells that shared HLA class I antigens with the donor and an HLA-A and -B negative human plasma cell line transfected with the HLA-A3.1 gene. The transfected cells were infectable by human immunodeficiency virus type 1 strains IIIB and MN but only the former virus sensitized them to killing by E7.20. The ability of E7.20 to specifically lyse a human Lymphocyte line infected with a human immunodeficiency virus type 1 strain carrying the conserved epitope is consistent with an important role for cytotoxic T cells in controlling infection.
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an hla c restricted cd8 cytotoxic t Lymphocyte Clone recognizes a highly conserved epitope on human immunodeficiency virus type 1 gag
Journal of Virology, 1991Co-Authors: Rebecca A Littaua, Michael B A Oldstone, Akira Takeda, Christine Debouck, Johnson T Wong, Carmelita U Tuazon, Bernard Moss, Femia Kievits, Francis A EnnisAbstract:A unique epitope on the gag protein of human immunodeficiency virus type 1 (HIV-1), located at amino acid 145 to 150, has been mapped by using a CD8+ cytotoxic T-Lymphocyte (CTL) Clone. This epitope is highly conserved among 18 HIV-1 strains. The HIV-1 gag-specific human leukocyte antigen (HLA) class I-restricted CD8+ CTL Clone was generated from fresh peripheral blood mononuclear cells of an HIV-seropositive donor by stimulation with gamma-irradiated allogeneic peripheral blood mononuclear cells in the presence of an anti-CD3 monoclonal antibody and recombinant interleukin-2. This gag-specific CTL Clone killed autologous target cells infected with a recombinant vaccinia virus containing the gag gene of HIV-1 and target cells pulsed with an authentic p24gag construct expressed in Escherichia coli. Fine specificity was determined by using a panel of overlapping 30-amino-acid-long synthetic peptides and subsequently using smaller peptides to precisely map the CTL domain on p24. The epitope is on a highly conserved region, and it overlaps with a major B-cell epitope of gag. This CD8+ T-cell epitope is restricted by HLA-Cw3, which has not been previously identified as a restricting element for human CTL responses.
Rebecca A Littaua - One of the best experts on this subject based on the ideXlab platform.
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a cd4 cytotoxic t Lymphocyte Clone to a conserved epitope on human immunodeficiency virus type 1 p24 cytotoxic activity and secretion of interleukin 2 and interleukin 6
Journal of Virology, 1992Co-Authors: Rebecca A Littaua, Michael B A Oldstone, Akira Takeda, Francis A EnnisAbstract:A CD4+ cytotoxic T-Lymphocyte (CTL) Clone, established from the peripheral blood of a human immunodeficiency virus (HIV)-seropositive donor, lysed autologous target cells that were infected with a recombinant vaccinia virus containing the gag gene of HIV type 1 and target cells pulsed with p24gag construct expressed in Escherichia coli. The recognition of the HLA-DQ-restricted epitope by this Clone was further defined by using overlapping synthetic peptides. The epitope recognized by this CD4+ CTL Clone (amino acids 140 to 148) overlaps with a CD8+ epitope and is highly conserved among all isolates of HIV type 1 that have been sequenced. Production and secretion of lymphokines such as interleukin-2 and interleukin-6 after specific antigenic stimulation were demonstrated by this gag-specific CD4+ CTL Clone.
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an hla c restricted cd8 cytotoxic t Lymphocyte Clone recognizes a highly conserved epitope on human immunodeficiency virus type 1 gag
Journal of Virology, 1991Co-Authors: Rebecca A Littaua, Michael B A Oldstone, Akira Takeda, Christine Debouck, Johnson T Wong, Carmelita U Tuazon, Bernard Moss, Femia Kievits, Francis A EnnisAbstract:A unique epitope on the gag protein of human immunodeficiency virus type 1 (HIV-1), located at amino acid 145 to 150, has been mapped by using a CD8+ cytotoxic T-Lymphocyte (CTL) Clone. This epitope is highly conserved among 18 HIV-1 strains. The HIV-1 gag-specific human leukocyte antigen (HLA) class I-restricted CD8+ CTL Clone was generated from fresh peripheral blood mononuclear cells of an HIV-seropositive donor by stimulation with gamma-irradiated allogeneic peripheral blood mononuclear cells in the presence of an anti-CD3 monoclonal antibody and recombinant interleukin-2. This gag-specific CTL Clone killed autologous target cells infected with a recombinant vaccinia virus containing the gag gene of HIV-1 and target cells pulsed with an authentic p24gag construct expressed in Escherichia coli. Fine specificity was determined by using a panel of overlapping 30-amino-acid-long synthetic peptides and subsequently using smaller peptides to precisely map the CTL domain on p24. The epitope is on a highly conserved region, and it overlaps with a major B-cell epitope of gag. This CD8+ T-cell epitope is restricted by HLA-Cw3, which has not been previously identified as a restricting element for human CTL responses.
Cornelis J M Melief - One of the best experts on this subject based on the ideXlab platform.
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spontaneous human squamous cell carcinomas are killed by a human cytotoxic t Lymphocyte Clone recognizing a wild type p53 derived peptide
Proceedings of the National Academy of Sciences of the United States of America, 1996Co-Authors: Mads Ropke, Jesper Hald, Per Guldberg, Jesper Zeuthen, Lars Norgaard, Lars Fugger, A Svejgaard, Sjoerd H Van Der Burg, Hans W Nijman, Cornelis J M MeliefAbstract:A cytotoxic T Lymphocyte (CTL) Clone generated in vitro from the peripheral blood of a healthy HLA-A2-positive individual against a synthetic p53 protein-derived wild-type peptide (L9V) was shown to kill squamous carcinoma cell lines derived from two head and neck carcinomas, which expressed mutant p53 genes, in a L9V/HLA-A2 specific and restricted fashion. Thus, the normal tolerance against endogenously processed p53 protein-derived self-epitopes can be broken by peptide-specific in vitro priming. p53 protein-derived wild-type peptides might thus represent tumor associated target molecules for immunotherapeutical approaches.
Bernard Moss - One of the best experts on this subject based on the ideXlab platform.
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specific lysis of human immunodeficiency virus type 1 infected cells by a hla a3 1 restricted cd8 cytotoxic t Lymphocyte Clone that recognizes a conserved peptide sequence within the gp41 subunit of the envelope protein
Proceedings of the National Academy of Sciences of the United States of America, 1991Co-Authors: Kazuo Takahashi, Bernard Moss, Li Chen Dai, Thomas R Fuerst, William E Biddison, Patricia L Earl, Francis A EnnisAbstract:Abstract A HLA-A3.1-restricted CD8+ cytotoxic T-cell Clone, E7.20, that lyses cells infected with human immunodeficiency virus type 1 was isolated from an infected individual. The epitope was localized to amino acids 768-778 (RLRDLLLIVTR, NL43 env sequence) of the cytoplasmic domain of gp41 by successive use of a panel of recombinant vaccinia viruses that express truncated env genes and synthetic peptides. The epitope is conserved on 7 (NL43, BRU, HXB2, BRVA, SC, JH3, and JFL) of 13 human immunodeficiency virus type 1 isolates from North America. Synthetic peptides of this region of strains RF and CDC4 are also recognized by E7.20 despite a nonconservative Thr----Val or Thr----Ala change at amino acid 777; however, an MN peptide, which has four amino acid substitutions, was not reactive. The epitope recognized by E7.20 has a predicted hydrophobic alpha-helical structure, with three contiguous Leu residues followed by Ile and Val at amino acids 772-776. Cytotoxicity was restricted by HLA-A3.1 using allogeneic target cells that shared HLA class I antigens with the donor and an HLA-A and -B negative human plasma cell line transfected with the HLA-A3.1 gene. The transfected cells were infectable by human immunodeficiency virus type 1 strains IIIB and MN but only the former virus sensitized them to killing by E7.20. The ability of E7.20 to specifically lyse a human Lymphocyte line infected with a human immunodeficiency virus type 1 strain carrying the conserved epitope is consistent with an important role for cytotoxic T cells in controlling infection.
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an hla c restricted cd8 cytotoxic t Lymphocyte Clone recognizes a highly conserved epitope on human immunodeficiency virus type 1 gag
Journal of Virology, 1991Co-Authors: Rebecca A Littaua, Michael B A Oldstone, Akira Takeda, Christine Debouck, Johnson T Wong, Carmelita U Tuazon, Bernard Moss, Femia Kievits, Francis A EnnisAbstract:A unique epitope on the gag protein of human immunodeficiency virus type 1 (HIV-1), located at amino acid 145 to 150, has been mapped by using a CD8+ cytotoxic T-Lymphocyte (CTL) Clone. This epitope is highly conserved among 18 HIV-1 strains. The HIV-1 gag-specific human leukocyte antigen (HLA) class I-restricted CD8+ CTL Clone was generated from fresh peripheral blood mononuclear cells of an HIV-seropositive donor by stimulation with gamma-irradiated allogeneic peripheral blood mononuclear cells in the presence of an anti-CD3 monoclonal antibody and recombinant interleukin-2. This gag-specific CTL Clone killed autologous target cells infected with a recombinant vaccinia virus containing the gag gene of HIV-1 and target cells pulsed with an authentic p24gag construct expressed in Escherichia coli. Fine specificity was determined by using a panel of overlapping 30-amino-acid-long synthetic peptides and subsequently using smaller peptides to precisely map the CTL domain on p24. The epitope is on a highly conserved region, and it overlaps with a major B-cell epitope of gag. This CD8+ T-cell epitope is restricted by HLA-Cw3, which has not been previously identified as a restricting element for human CTL responses.
Michael B A Oldstone - One of the best experts on this subject based on the ideXlab platform.
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a cd4 cytotoxic t Lymphocyte Clone to a conserved epitope on human immunodeficiency virus type 1 p24 cytotoxic activity and secretion of interleukin 2 and interleukin 6
Journal of Virology, 1992Co-Authors: Rebecca A Littaua, Michael B A Oldstone, Akira Takeda, Francis A EnnisAbstract:A CD4+ cytotoxic T-Lymphocyte (CTL) Clone, established from the peripheral blood of a human immunodeficiency virus (HIV)-seropositive donor, lysed autologous target cells that were infected with a recombinant vaccinia virus containing the gag gene of HIV type 1 and target cells pulsed with p24gag construct expressed in Escherichia coli. The recognition of the HLA-DQ-restricted epitope by this Clone was further defined by using overlapping synthetic peptides. The epitope recognized by this CD4+ CTL Clone (amino acids 140 to 148) overlaps with a CD8+ epitope and is highly conserved among all isolates of HIV type 1 that have been sequenced. Production and secretion of lymphokines such as interleukin-2 and interleukin-6 after specific antigenic stimulation were demonstrated by this gag-specific CD4+ CTL Clone.
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an hla c restricted cd8 cytotoxic t Lymphocyte Clone recognizes a highly conserved epitope on human immunodeficiency virus type 1 gag
Journal of Virology, 1991Co-Authors: Rebecca A Littaua, Michael B A Oldstone, Akira Takeda, Christine Debouck, Johnson T Wong, Carmelita U Tuazon, Bernard Moss, Femia Kievits, Francis A EnnisAbstract:A unique epitope on the gag protein of human immunodeficiency virus type 1 (HIV-1), located at amino acid 145 to 150, has been mapped by using a CD8+ cytotoxic T-Lymphocyte (CTL) Clone. This epitope is highly conserved among 18 HIV-1 strains. The HIV-1 gag-specific human leukocyte antigen (HLA) class I-restricted CD8+ CTL Clone was generated from fresh peripheral blood mononuclear cells of an HIV-seropositive donor by stimulation with gamma-irradiated allogeneic peripheral blood mononuclear cells in the presence of an anti-CD3 monoclonal antibody and recombinant interleukin-2. This gag-specific CTL Clone killed autologous target cells infected with a recombinant vaccinia virus containing the gag gene of HIV-1 and target cells pulsed with an authentic p24gag construct expressed in Escherichia coli. Fine specificity was determined by using a panel of overlapping 30-amino-acid-long synthetic peptides and subsequently using smaller peptides to precisely map the CTL domain on p24. The epitope is on a highly conserved region, and it overlaps with a major B-cell epitope of gag. This CD8+ T-cell epitope is restricted by HLA-Cw3, which has not been previously identified as a restricting element for human CTL responses.
