The Experts below are selected from a list of 3483 Experts worldwide ranked by ideXlab platform
Robert P Perrillo - One of the best experts on this subject based on the ideXlab platform.
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rosette inhibitory factor t Lymphocyte Subpopulation specificity and potential immunoregulatory role in hepatitis b virus infection
Hepatology, 2007Co-Authors: Gary E Sanders, Robert P PerrilloAbstract:We have observed the disappearance of rosette inhibitory factor (RIF) from the serum of 19 patients with acute hepatitis B virus infection. This occurred at a time coinciding with the detection of anti-HBs. In addition, levels of RIF activity were significantly greater (p < 0.001) in 35 HBsAg carriers who lacked anti-HBs when compared to 15 carriers who regularly demonstrated this antibody. In all instances, RIF effect was partial affecting some, but not all, T-Lymphocytes from forming erythrocyte rosettes. To define if RIF exhibits an effect on specific T-Lymphocyte Subpopulations, Lymphocytes from healthy donors were separated into TM (helper), TG (suppressor), and To (null) Subpopulations by an immunoglobulin-ox-cell rosette depletion method. The effect of RIF on erythrocyte rosette formation and Fc-receptor expression in these Subpopulations was assessed. TG-Lymphocytes were found to be refractory to RIF-mediated suppression of erythrocyte rosette formation while TM-Lymphocytes demonstrated an enhanced sensitivity to RIF. Incubation of TG- and To-Lymphocytes, potential TM-precursor cells, with RIF resulted in a decreased expression of new IgM-Fc receptors. In order to determine if any functional significance could be derived from these findings, the effect of RIF on in vitro immunoglobulin secretion was tested. Using pokeweed mitogen-stimulated mononuclear cell cultures, purified RIF-low density lipoprotein was shown to suppress IgM, IgG, and IgA secretion by 75.3, 74.3, and 59.3%, respectively. These data are consistent with the hypothesis that RIF is a potential immunoregulatory protein which could contribute to the lack of anti-HBs noted during the acute phase of hepatitis B and in the majority of HBsAg carriers.
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rosette inhibitory factor t Lymphocyte Subpopulation specificity and potential immunoregulatory role in hepatitis b virus infection
Hepatology, 2007Co-Authors: Gary E Sanders, Robert P PerrilloAbstract:We have observed the disappearance of rosette inhibitory factor (RIF) from the serum of 19 patients with acute hepatitis B virus infection. This occurred at a time coinciding with the detection of anti-HBs. In addition, levels of RIF activity were significantly greater (P less than 0.001) in 35 HBsAg carriers who lacked anti-HBs when compared to 15 carriers who regularly demonstrated this antibody. In all instances, RIF effect was partial affecting some, but not all, T-Lymphocytes from healthy donors were separated into TM (helper), TG (suppressor), and T0 (null) Subpopulations by an immunoglobulin-ox-cell rosette depletion method. The effect of RIF on erythrocyte rosette formation and Fc-receptor expression in these Subpopulations was assessed. TG-Lymphocytes were found to be refractory to RIF-mediated suppression of erythrocyte rosette formation while TM-Lymphocytes demonstrated an enhanced sensitivity to RIF. Incubation of TG-Lymphocytes, potential TM-percursor cells, with RIF resulted in a decreased expression of new IgM-Fc receptors. In order to determine if any functional significance could be derived from these findings, the effect of RIF on in vitro immunoglobulin secretion was tested. Using pokeweed mitogen-stimulated mononuclear cell cultures, purified RIF-low density lipoprotein was shown to suppress IgM, IgG, and IgA secretion by 75.3, 74.3, and 59.3%, respectively. These data are consistent with the hypothesis that RIF is a potential immunoregulatory protein which could contribute to the lack of anti-HBs noted during the acute phase of hepatitis B and in the majority of HBsAg carriers.
Gary E Sanders - One of the best experts on this subject based on the ideXlab platform.
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rosette inhibitory factor t Lymphocyte Subpopulation specificity and potential immunoregulatory role in hepatitis b virus infection
Hepatology, 2007Co-Authors: Gary E Sanders, Robert P PerrilloAbstract:We have observed the disappearance of rosette inhibitory factor (RIF) from the serum of 19 patients with acute hepatitis B virus infection. This occurred at a time coinciding with the detection of anti-HBs. In addition, levels of RIF activity were significantly greater (p < 0.001) in 35 HBsAg carriers who lacked anti-HBs when compared to 15 carriers who regularly demonstrated this antibody. In all instances, RIF effect was partial affecting some, but not all, T-Lymphocytes from forming erythrocyte rosettes. To define if RIF exhibits an effect on specific T-Lymphocyte Subpopulations, Lymphocytes from healthy donors were separated into TM (helper), TG (suppressor), and To (null) Subpopulations by an immunoglobulin-ox-cell rosette depletion method. The effect of RIF on erythrocyte rosette formation and Fc-receptor expression in these Subpopulations was assessed. TG-Lymphocytes were found to be refractory to RIF-mediated suppression of erythrocyte rosette formation while TM-Lymphocytes demonstrated an enhanced sensitivity to RIF. Incubation of TG- and To-Lymphocytes, potential TM-precursor cells, with RIF resulted in a decreased expression of new IgM-Fc receptors. In order to determine if any functional significance could be derived from these findings, the effect of RIF on in vitro immunoglobulin secretion was tested. Using pokeweed mitogen-stimulated mononuclear cell cultures, purified RIF-low density lipoprotein was shown to suppress IgM, IgG, and IgA secretion by 75.3, 74.3, and 59.3%, respectively. These data are consistent with the hypothesis that RIF is a potential immunoregulatory protein which could contribute to the lack of anti-HBs noted during the acute phase of hepatitis B and in the majority of HBsAg carriers.
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rosette inhibitory factor t Lymphocyte Subpopulation specificity and potential immunoregulatory role in hepatitis b virus infection
Hepatology, 2007Co-Authors: Gary E Sanders, Robert P PerrilloAbstract:We have observed the disappearance of rosette inhibitory factor (RIF) from the serum of 19 patients with acute hepatitis B virus infection. This occurred at a time coinciding with the detection of anti-HBs. In addition, levels of RIF activity were significantly greater (P less than 0.001) in 35 HBsAg carriers who lacked anti-HBs when compared to 15 carriers who regularly demonstrated this antibody. In all instances, RIF effect was partial affecting some, but not all, T-Lymphocytes from healthy donors were separated into TM (helper), TG (suppressor), and T0 (null) Subpopulations by an immunoglobulin-ox-cell rosette depletion method. The effect of RIF on erythrocyte rosette formation and Fc-receptor expression in these Subpopulations was assessed. TG-Lymphocytes were found to be refractory to RIF-mediated suppression of erythrocyte rosette formation while TM-Lymphocytes demonstrated an enhanced sensitivity to RIF. Incubation of TG-Lymphocytes, potential TM-percursor cells, with RIF resulted in a decreased expression of new IgM-Fc receptors. In order to determine if any functional significance could be derived from these findings, the effect of RIF on in vitro immunoglobulin secretion was tested. Using pokeweed mitogen-stimulated mononuclear cell cultures, purified RIF-low density lipoprotein was shown to suppress IgM, IgG, and IgA secretion by 75.3, 74.3, and 59.3%, respectively. These data are consistent with the hypothesis that RIF is a potential immunoregulatory protein which could contribute to the lack of anti-HBs noted during the acute phase of hepatitis B and in the majority of HBsAg carriers.
Navarro-barriuso Juan - One of the best experts on this subject based on the ideXlab platform.
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Th1Th17 CM Lymphocyte Subpopulation as a predictive biomarker of disease activity in multiple sclerosis patients under dimethyl fumarate or fingolimod treatment
'Hindawi Limited', 2019Co-Authors: Quirant-sánchez Bibiana, Presas-rodriguez Silvia, Mansilla, María José, Teniente-serra Aina, Hervás-garcía José, Brieva Luis, Moral-torres Ester, Cano Antonio, Munteis Olivas Elvira, Navarro-barriuso JuanAbstract:Peripheral blood biomarkers able to predict disease activity in multiple sclerosis (MS) patients have not been identified yet. Here, we analyzed the immune phenotype of T Lymphocyte Subpopulations in peripheral blood samples from 66 RRMS patients under DMF (n = 22) or fingolimod (n = 44) treatment, by flow cytometry. A correlation study between the percentage and absolute cell number of each Lymphocyte Subpopulation with the presence of relapses or new MRI lesions during 12-month follow-up was performed. Patients who had undergone relapses showed at baseline higher percentage of Th1CM cells (relapsed: 11.60 ± 4.17%vs. nonrelapsed: 9.25 ± 3.17%, p 11.48% had a 50% relapse-free survival compared to patients with Th1Th17CMcells < 11.48% whose relapse-free survival was 88% (p = 0.013, log-rank test). Additionally, a high percentage of Th1Th17CM cells was also found in patients with MRI activity (MRI activity: 14.02 ± 5.87%vs. no MRI activity: 9.82 ± 4.06%, p < 0.01). Our results suggest that the percentage of Th1Th17CM Lymphocytes at baseline is a predictive biomarker of activity during the first 12 months of treatment, regardless of the treatment
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Th1Th17CM Lymphocyte Subpopulation as a predictive biomarker of disease activity in multiple sclerosis patients under dimethyl fumarate or fingolimod treatment
'Hindawi Limited', 2019Co-Authors: Quirant-sánchez Bibiana, Presas-rodriguez Silvia, Teniente-serra Aina, Brieva Luis, Moral-torres Ester, Cano Antonio, Mansilla Lopez, Maria Jose, Hervas-garcia, Jose Vicente, Munteis Elvira, Navarro-barriuso JuanAbstract:This work has been supported by positive discussion through ENTIRE European network (BM0907; https://www.cost.eu/ actions/BM0907) and A FACTT network (COST Action BM1305: www.afactt.eu). COST is supported by the EU Framework Programme Horizon 2020. We thank Mr. Marco A. Fernández of the Cytometry Facility of IGTP for his continuous help and suggestions and Ms. Amanda Rus for her technical assistance. The authors are members of a consolidated group (02/2015-01/2018) as recognized by the Agency for Management of University and Research Grants (AGAUR) of the Generalitat of Catalonia. This study was sponsored in part by Novartis Pharmaceuticals Corporation and in part by the Spanish grants FIS PI14/01175 and PI16/01737, integrated in the Plan Nacional de I+D+I and cosupported by the ISCIII-Subdirección General de Evalua-ción and the Fondo Europeo de Desarrollo Regional (FEDER).Peripheral blood biomarkers able to predict disease activity in multiple sclerosis (MS) patients have not been identified yet. Here, we analyzed the immune phenotype of T Lymphocyte Subpopulations in peripheral blood samples from 66 RRMS patients under DMF (n = 22) or fingolimod (n = 44) treatment, by flow cytometry. A correlation study between the percentage and absolute cell number of each Lymphocyte Subpopulation with the presence of relapses or new MRI lesions during 12-month follow-up was performed. Patients who had undergone relapses showed at baseline higher percentage of Th1 cells (relapsed: 11 60 ± 4 17%vs. nonrelapsed: 9 25 ± 3 17%, p 11 48% had a 50% relapse-free survival compared to patients with Th1Th17 cells < 11 48% whose relapse-free survival was 88% (p = 0 013, log-rank test). Additionally, a high percentage of Th1Th17 cells was also found in patients with MRI activity (MRI activity: 14 02 ± 5 87%vs. no MRI activity: 9 82 ± 4 06%, p < 0 01). Our results suggest that the percentage of Th1Th17 Lymphocytes at baseline is a predictive biomarker of activity during the first 12 months of treatment, regardless of the treatment
Kang D. Choi - One of the best experts on this subject based on the ideXlab platform.
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Eimeria tenella Infection Induces Local Gamma Interferon Production and Intestinal Lymphocyte Subpopulation Changes
Infection and immunity, 2000Co-Authors: Cheol H. Yun, Hyun S. Lillehoj, Kang D. ChoiAbstract:The role of intestinal Lymphocytes and gamma interferon (IFN-γ) production in protective immunity to Eimeria tenella infection was evaluated in two inbred strains of chickens (SC and TK) that display different patterns of susceptibility to coccidiosis. Oral inoculation of either strain with E. tenella led to parasite invasion of the intestinal cecum and cecal tonsils. Greater fecal oocyst shedding was seen in TK chickens. Flow cytometric analyses of cecal tonsil Lymphocytes demonstrated greater numbers of CD4+ and T-cell receptor γδ-positive (TCR1+) cells in SC chickens and elevated numbers of CD8+ and TCR2+ cells in TK chickens following primary infection. IFN-γ mRNA expression was significantly increased in cecal tonsil and intraepithelial Lymphocytes at days 6 and 8, respectively, after primary infection in SC compared to TK chickens. While no differences were noted between cecal tonsil Lymphocytes of the two strains following secondary infection, TK chickens showed elevated IFN-γ transcript levels in intestinal intraepithelial Lymphocytes at this time. Selective depletion of CD4+, but not CD8+, cecal tonsil Lymphocytes in SC chickens resulted in a reduced IFN-γ mRNA expression, indicating that CD4+ cells are the primary source of this cytokine. Collectively, these results indicate that local Lymphocyte responses and production of IFN-γ are influenced by host genetic factors.
Zheng Xiaoye - One of the best experts on this subject based on the ideXlab platform.
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method evaluation and preliminary application of antibody coating immunomagnetic beads to isolate cd4 t Lymphocyte Subpopulation
Chinese Journal of Infection Control, 2005Co-Authors: Zheng XiaoyeAbstract:Objective To isolate CD4+ T Lymphocyte Subpopulation by antibody-coating immunomagnetic beads and evaluate the efficacy of detective methods. Methods Immunomagnetic beads were prepared by direct antibody coating and analysed by flow cytometry. Results The isolation rate of CD4+ T Lymphocyte Subpopulation was above 95%. Conclusion Direct antibody coating of immunomagnetic beads can isolate CD4+ T Lymphocyte Subpopulation effectively.
