The Experts below are selected from a list of 228 Experts worldwide ranked by ideXlab platform
Christian Périgaud - One of the best experts on this subject based on the ideXlab platform.
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Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intraCellular 1-beta-d-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicu
Journal of Chromatography B Biomedical Sciences and Applications, 2009Co-Authors: Céline Crauste, Isabelle Lefebvre, Michael Hovaneissian, Jean Puy, Béatrice Roy, Suzanne Peyrottes, Sabine Cohen, Jérome Guitton, Charles Dumontet, Christian PérigaudAbstract:Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intraCellular 1-beta-d-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicular Lymphoma Cell Line a b s t r a c t A method was developed for the quantification of araCTP, CTP and dCTP in a human follicular Lymphoma Cell Line. This method involves solid phase extraction (SPE) using a weak anion-exchanger (WAX) cartridge , a porous graphitic carbon high-performance liquid chromatography (HPLC) column separation, and tandem mass spectrometry (MS/MS) detection. By using a triple quadrupole mass spectrometer operating in negative ion multiple reaction monitoring (MRM) mode, the method was able to achieve a lower limit of quantification (LLOQ) of 0.1 g mL −1 for araCTP and of 0.01 g mL −1 for both CTP and dCTP. The method was validated and used to determine the amount of araCTP, CTP and dCTP formed after incubation of araC and an araCMP prodrug in the human follicular Lymphoma Cell Line RL.
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Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intraCellular 1-beta-D-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicu
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2009Co-Authors: Céline Crauste, Isabelle Lefebvre, Michael Hovaneissian, Jean Puy, Béatrice Roy, Suzanne Peyrottes, Sabine Cohen, Jérome Guitton, Charles Dumontet, Christian PérigaudAbstract:A method was developed for the quantification of araCTP, CTP and dCTP in a human follicular Lymphoma Cell Line. This method involves solid phase extraction (SPE) using a weak anion-exchanger (WAX) cartridge, a porous graphitic carbon high-performance liquid chromatography (HPLC) column separation, and tandem mass spectrometry (MS/MS) detection. By using a triple quadrupole mass spectrometer operating in negative ion multiple reaction monitoring (MRM) mode, the method was able to achieve a lower limit of quantification (LLOQ) of 0.1 microg mL(-1) for araCTP and of 0.01 microg mL(-1) for both CTP and dCTP. The method was validated and used to determine the amount of araCTP, CTP and dCTP formed after incubation of araC and an araCMP prodrug in the human follicular Lymphoma Cell Line RL.
Céline Crauste - One of the best experts on this subject based on the ideXlab platform.
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Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intraCellular 1-beta-d-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicu
Journal of Chromatography B Biomedical Sciences and Applications, 2009Co-Authors: Céline Crauste, Isabelle Lefebvre, Michael Hovaneissian, Jean Puy, Béatrice Roy, Suzanne Peyrottes, Sabine Cohen, Jérome Guitton, Charles Dumontet, Christian PérigaudAbstract:Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intraCellular 1-beta-d-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicular Lymphoma Cell Line a b s t r a c t A method was developed for the quantification of araCTP, CTP and dCTP in a human follicular Lymphoma Cell Line. This method involves solid phase extraction (SPE) using a weak anion-exchanger (WAX) cartridge , a porous graphitic carbon high-performance liquid chromatography (HPLC) column separation, and tandem mass spectrometry (MS/MS) detection. By using a triple quadrupole mass spectrometer operating in negative ion multiple reaction monitoring (MRM) mode, the method was able to achieve a lower limit of quantification (LLOQ) of 0.1 g mL −1 for araCTP and of 0.01 g mL −1 for both CTP and dCTP. The method was validated and used to determine the amount of araCTP, CTP and dCTP formed after incubation of araC and an araCMP prodrug in the human follicular Lymphoma Cell Line RL.
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Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intraCellular 1-beta-D-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicu
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2009Co-Authors: Céline Crauste, Isabelle Lefebvre, Michael Hovaneissian, Jean Puy, Béatrice Roy, Suzanne Peyrottes, Sabine Cohen, Jérome Guitton, Charles Dumontet, Christian PérigaudAbstract:A method was developed for the quantification of araCTP, CTP and dCTP in a human follicular Lymphoma Cell Line. This method involves solid phase extraction (SPE) using a weak anion-exchanger (WAX) cartridge, a porous graphitic carbon high-performance liquid chromatography (HPLC) column separation, and tandem mass spectrometry (MS/MS) detection. By using a triple quadrupole mass spectrometer operating in negative ion multiple reaction monitoring (MRM) mode, the method was able to achieve a lower limit of quantification (LLOQ) of 0.1 microg mL(-1) for araCTP and of 0.01 microg mL(-1) for both CTP and dCTP. The method was validated and used to determine the amount of araCTP, CTP and dCTP formed after incubation of araC and an araCMP prodrug in the human follicular Lymphoma Cell Line RL.
Sun Zhenchang - One of the best experts on this subject based on the ideXlab platform.
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Effect and mechanism of Prunella vulgaris L extract on proliferation of human Burkitt Lymphoma Cell Line Raji
Shandong Medical Journal, 2008Co-Authors: Sun ZhenchangAbstract:Objective To observe the effects of the extract from Prunella vulgaris L on human Burkitt Lymphoma Cell Line Raji. Methods The effects of the extract from Prunella vulgaris L on Raji Cells,proliferation was detected with MTT. Apoptosis of Raji Cells was observed by Cell morphology and agarose gel electrophoresis. The apoptosis rate of Raji Cells were detected by flow cytometry.Results The different doses of the extract from Prunella vulgaris L could remarkably inhibit the proliferation of Raji Cells with dose dependent,and the IC50 was (18.01±0.92) μg/ml. The apoptosis strap was widen and brighten,and the apoptosis rate heightened with time.Conclusion The extract from Prunella vulgaris L can inhibit the proliferation of Raji Cells by inducing apoptosis probably.
Martha M. Moore - One of the best experts on this subject based on the ideXlab platform.
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Multicolor spectral karyotyping of the L5178Y Tk+/−‐3.7.2C mouse Lymphoma Cell Line
Environmental and molecular mutagenesis, 2006Co-Authors: Jeffrey R. Sawyer, Regina Lichti Binz, Jianyong Wang, Martha M. MooreAbstract:The L5178Y/Tk+/−-3.7.2C mouse Lymphoma Cell Line is characterized, at the cytogenetic level, by a karyotype involving both numerical and complex structural aberrations. While the karyotype is remarkably normal for a transformed Cell Line that has been in culture for almost half a century, there are a number of chromosomal alterations that because of their complexity cannot be fully characterized by routine or even high-resolution G-banding studies. Multicolor spectral karyotyping (SKY) was performed on the Cell Line in anticipation of identifying the previously unresolved chromosome aberrations and confirming interpretations previously identified by banding studies. New chromosome aberrations detected by SKY include numerical aberrations of chromosome 15, duplications of regions of chromosomes 4, 5, 12, and 18, and deletion of chromosome 14. Complex unbalanced translocations involved segments of chromosomes 6, 14, and 15. In total, the SKY technique was able to provide new refined designations on segments of eight different chromosome pairs (4, 5, 6, 9, 12, 14, 15, 18) and identified all three previously unidentified marker chromosomes. This analysis provides an updated standard reference for the karyotype of the L5178Y/Tk+/−-3.7.2C Cell Line used in the in vitro mouse Lymphoma mutation assay. Environ. Mol. Mutagen., 2006. © 2005 Wiley-Liss, Inc.
Béatrice Roy - One of the best experts on this subject based on the ideXlab platform.
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Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intraCellular 1-beta-d-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicu
Journal of Chromatography B Biomedical Sciences and Applications, 2009Co-Authors: Céline Crauste, Isabelle Lefebvre, Michael Hovaneissian, Jean Puy, Béatrice Roy, Suzanne Peyrottes, Sabine Cohen, Jérome Guitton, Charles Dumontet, Christian PérigaudAbstract:Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intraCellular 1-beta-d-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicular Lymphoma Cell Line a b s t r a c t A method was developed for the quantification of araCTP, CTP and dCTP in a human follicular Lymphoma Cell Line. This method involves solid phase extraction (SPE) using a weak anion-exchanger (WAX) cartridge , a porous graphitic carbon high-performance liquid chromatography (HPLC) column separation, and tandem mass spectrometry (MS/MS) detection. By using a triple quadrupole mass spectrometer operating in negative ion multiple reaction monitoring (MRM) mode, the method was able to achieve a lower limit of quantification (LLOQ) of 0.1 g mL −1 for araCTP and of 0.01 g mL −1 for both CTP and dCTP. The method was validated and used to determine the amount of araCTP, CTP and dCTP formed after incubation of araC and an araCMP prodrug in the human follicular Lymphoma Cell Line RL.
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Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intraCellular 1-beta-D-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicu
Journal of chromatography. B Analytical technologies in the biomedical and life sciences, 2009Co-Authors: Céline Crauste, Isabelle Lefebvre, Michael Hovaneissian, Jean Puy, Béatrice Roy, Suzanne Peyrottes, Sabine Cohen, Jérome Guitton, Charles Dumontet, Christian PérigaudAbstract:A method was developed for the quantification of araCTP, CTP and dCTP in a human follicular Lymphoma Cell Line. This method involves solid phase extraction (SPE) using a weak anion-exchanger (WAX) cartridge, a porous graphitic carbon high-performance liquid chromatography (HPLC) column separation, and tandem mass spectrometry (MS/MS) detection. By using a triple quadrupole mass spectrometer operating in negative ion multiple reaction monitoring (MRM) mode, the method was able to achieve a lower limit of quantification (LLOQ) of 0.1 microg mL(-1) for araCTP and of 0.01 microg mL(-1) for both CTP and dCTP. The method was validated and used to determine the amount of araCTP, CTP and dCTP formed after incubation of araC and an araCMP prodrug in the human follicular Lymphoma Cell Line RL.
