The Experts below are selected from a list of 66 Experts worldwide ranked by ideXlab platform
Cheng Huang Lin - One of the best experts on this subject based on the ideXlab platform.
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Optimization of the separation of Lysergic Acid Diethylamide in urine by a sweeping technique using micellar electrokinetic chromatography
Journal of Chromatography B: Biomedical Sciences and Applications, 2002Co-Authors: Ching Fang, Ju-tsung Liu, Cheng Huang LinAbstract:The separation and on-line concentrations of Lysergic Acid Diethylamide (LSD), iso-Lysergic Acid Diethylamide (iso-LSD) and Lysergic Acid N,N-methy(propylamide (LAMPA) in human urine were investigated by capillary electrophoresis-fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as an anionic surfactant. A number of parameters such as buffer pH, SDS concentration. Brij-30 concentration and the content of organic solvent used in separation, were optimized. The techniques of sweeping-micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were used for determining on-line concentrations. The advantages and disadvantages of this procedure with respect to sensitivity, precision and simplicity are discussed and compared.
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O ptimization of the separation of Lysergic Acid Diethylamide in urine by a sweeping technique using micellar electrokinetic
2002Co-Authors: Ching Fang, Ju-tsung Liu, Cheng Huang LinAbstract:The separation and on-line concentrations of Lysergic Acid Diethylamide (LSD), iso-Lysergic Acid Diethylamide (iso-LSD) and Lysergic Acid N,N-methylpropylamide (LAMPA) in human urine were investigated by capillary electrophoresis– fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as an anionic surfactant. A number of parameters such as buffer pH, SDS concentration, Brij-30 concentration and the content of organic solvent used in separation, were optimized. The techniques of sweeping-micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were used for determining on-line concentrations. The advantages and disadvantages of this procedure with respect to sensitivity, precision and simplicity are discussed and compared. 2002 Elsevier Science B.V. All rights reserved.
Agnieszka Nikiforuk - One of the best experts on this subject based on the ideXlab platform.
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serotonin type 5a receptor antagonists inhibit d Lysergic Acid Diethylamide discriminatory cue in rats
Journal of Psychopharmacology, 2019Co-Authors: Piotr Popik, Martyna Krawczyk, Agata Kuziak, Ryszard Bugno, Adam S Hogendorf, Jakub Staron, Agnieszka NikiforukAbstract:Purpose:Like other psychedelics, D-Lysergic Acid Diethylamide (LSD) affects numerous serotonin receptors, and according to the current dogma, the 5-HT2A receptors are considered the main target for...
Ching Fang - One of the best experts on this subject based on the ideXlab platform.
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Optimization of the separation of Lysergic Acid Diethylamide in urine by a sweeping technique using micellar electrokinetic chromatography
Journal of Chromatography B: Biomedical Sciences and Applications, 2002Co-Authors: Ching Fang, Ju-tsung Liu, Cheng Huang LinAbstract:The separation and on-line concentrations of Lysergic Acid Diethylamide (LSD), iso-Lysergic Acid Diethylamide (iso-LSD) and Lysergic Acid N,N-methy(propylamide (LAMPA) in human urine were investigated by capillary electrophoresis-fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as an anionic surfactant. A number of parameters such as buffer pH, SDS concentration. Brij-30 concentration and the content of organic solvent used in separation, were optimized. The techniques of sweeping-micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were used for determining on-line concentrations. The advantages and disadvantages of this procedure with respect to sensitivity, precision and simplicity are discussed and compared.
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O ptimization of the separation of Lysergic Acid Diethylamide in urine by a sweeping technique using micellar electrokinetic
2002Co-Authors: Ching Fang, Ju-tsung Liu, Cheng Huang LinAbstract:The separation and on-line concentrations of Lysergic Acid Diethylamide (LSD), iso-Lysergic Acid Diethylamide (iso-LSD) and Lysergic Acid N,N-methylpropylamide (LAMPA) in human urine were investigated by capillary electrophoresis– fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as an anionic surfactant. A number of parameters such as buffer pH, SDS concentration, Brij-30 concentration and the content of organic solvent used in separation, were optimized. The techniques of sweeping-micellar electrokinetic chromatography (sweeping-MEKC) and cation-selective exhaustive injection-sweep-micellar electrokinetic chromatography (CSEI-sweep-MEKC) were used for determining on-line concentrations. The advantages and disadvantages of this procedure with respect to sensitivity, precision and simplicity are discussed and compared. 2002 Elsevier Science B.V. All rights reserved.
Piotr Popik - One of the best experts on this subject based on the ideXlab platform.
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serotonin type 5a receptor antagonists inhibit d Lysergic Acid Diethylamide discriminatory cue in rats
Journal of Psychopharmacology, 2019Co-Authors: Piotr Popik, Martyna Krawczyk, Agata Kuziak, Ryszard Bugno, Adam S Hogendorf, Jakub Staron, Agnieszka NikiforukAbstract:Purpose:Like other psychedelics, D-Lysergic Acid Diethylamide (LSD) affects numerous serotonin receptors, and according to the current dogma, the 5-HT2A receptors are considered the main target for...
E Sanders-bush - One of the best experts on this subject based on the ideXlab platform.
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Unsurmountable antagonism of brain 5-hydroxytryptamine2 receptors by (+)-Lysergic Acid Diethylamide and bromo-Lysergic Acid Diethylamide.
Molecular pharmacology, 1992Co-Authors: K D Burris, E Sanders-bushAbstract:Lysergic Acid Diethylamide (LSD) and its structural analogue 2-bromo-Lysergic Acid Diethylamide (BOL) act as unsurmountable antagonists of serotonin-elicited contractions in smooth muscle preparations. Two different models, allosteric and kinetic, have been invoked to explain these findings. The present studies investigate the mechanism of antagonism of brain 5-hydroxytryptamine (5HT)2 receptors, utilizing cells transfected with 5HT2 receptor cDNA cloned from rat brain. A proximal cellular response, phosphoinositide hydrolysis, was examined in order to minimize possible postreceptor effects. Even though LSD behaved as a partial agonist and BOL as a pure antagonist, both drugs blocked the effect of serotonin in an unsurmountable manner, i.e., increasing concentrations of serotonin could not overcome the blocking effect of LSD or BOL. Radioligand binding studies showed that preincubation of membranes with either LSD or BOL reduced the density of [3H]ketanserin binding sites, suggesting that the drugs bind tightly to the 5HT2 receptor and are not displaced during the binding assay. Two additional experiments supported this hypothesis. First, the off-rate of [3H] LSD was slow (20 min), relative to that of [3H]ketanserin (approximately 4 min). Second, when the length of incubation with [3H]ketanserin was increased to 60 min, the LSD-induced decrease in Bmax was essentially eliminated. The possibility that LSD and BOL decrease [3H]ketanserin binding by interacting with an allosteric site was rejected, because neither drug altered the rate of dissociation of [3H]ketanserin. The most parsimonious interpretation of these results is that unsurmountable antagonism reflects prolonged occupancy of the receptor by slowly reversible antagonists.
