Lysogeny Broth

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Reza Ghodssi - One of the best experts on this subject based on the ideXlab platform.

  • An ALD aluminum oxide passivated Surface Acoustic Wave sensor for early biofilm detection
    Sensors and Actuators B-chemical, 2012
    Co-Authors: Saeed Esmaili Sardari, A. A. Iliadis, Hsuan Chen Wu, Mariana T. Meyer, William E. Bentley, Reza Ghodssi
    Abstract:

    Abstract We present a successful demonstration of a reusable Surface Acoustic Wave (SAW) sensor for bacterial biofilm growth monitoring in an animal serum and bacterial growth media. Bacterial biofilms produce harmful metabolic by-products and are a characteristic of severe infections. Thus, continuous monitoring of bacterial biofilm growth is critical. Here, we report a highly sensitive SAW sensor for biofilm growth monitoring fabricated by depositing zinc oxide (ZnO) piezoelectric thin film by pulsed laser deposition (PLD). To prevent ZnO damage from long term exposure to bacterial growth media or to an animal serum, the ZnO layer of the sensor was effectively protected by aluminum oxide (Al 2 O 3 ) using atomic layer deposition (ALD). As a result, the sensor was reusable for consecutive biofilm formation experiments. The detection limit of the SAW sensor was approximately 5.3 pg. The SAW sensor was tested with Escherichia coli W3110 in Lysogeny Broth (LB) media, and in 10% diluted Fetal Bovine Serum (FBS) as an approximation to an in vivo environment. The resonant frequency shift measured at the output of the SAW sensor in both LB media and 10% FBS corresponded to natural biofilm growth. These repeatable results support the novel application of a SAW sensor for real time biofilm sensing.

  • An ALD aluminum oxide passivated Surface Acoustic Wave sensor for early biofilm detection
    Sensors and Actuators B: Chemical, 2012
    Co-Authors: Young-wook Kim, Saeed Esmaili Sardari, A. A. Iliadis, Hsuan Chen Wu, Mariana T. Meyer, William E. Bentley, Reza Ghodssi
    Abstract:

    We present a successful demonstration of a reusable Surface Acoustic Wave (SAW) sensor for bacterial biofilm growth monitoring in an animal serum and bacterial growth media. Bacterial biofilms produce harmful metabolic by-products and are a characteristic of severe infections. Thus, continuous monitoring of bacterial biofilm growth is critical. Here, we report a highly sensitive SAW sensor for biofilm growth monitoring fabricated by depositing zinc oxide (ZnO) piezoelectric thin film by pulsed laser deposition (PLD). To prevent ZnO damage from long term exposure to bacterial growth media or to an animal serum, the ZnO layer of the sensor was effectively protected by aluminum oxide (Al2O3) using atomic layer deposition (ALD). As a result, the sensor was reusable for consecutive biofilm formation experiments. The detection limit of the SAW sensor was approximately 5.3 pg. The SAW sensor was tested with Escherichia coli W3110 in Lysogeny Broth (LB) media, and in 10% diluted Fetal Bovine Serum (FBS) as an approximation to an in vivo environment. The resonant frequency shift measured at the output of the SAW sensor in both LB media and 10% FBS corresponded to natural biofilm growth. These repeatable results support the novel application of a SAW sensor for real time biofilm sensing. © 2012 Elsevier B.V. All rights reserved.

Young-wook Kim - One of the best experts on this subject based on the ideXlab platform.

  • An ALD aluminum oxide passivated Surface Acoustic Wave sensor for early biofilm detection
    Sensors and Actuators B: Chemical, 2012
    Co-Authors: Young-wook Kim, Saeed Esmaili Sardari, A. A. Iliadis, Hsuan Chen Wu, Mariana T. Meyer, William E. Bentley, Reza Ghodssi
    Abstract:

    We present a successful demonstration of a reusable Surface Acoustic Wave (SAW) sensor for bacterial biofilm growth monitoring in an animal serum and bacterial growth media. Bacterial biofilms produce harmful metabolic by-products and are a characteristic of severe infections. Thus, continuous monitoring of bacterial biofilm growth is critical. Here, we report a highly sensitive SAW sensor for biofilm growth monitoring fabricated by depositing zinc oxide (ZnO) piezoelectric thin film by pulsed laser deposition (PLD). To prevent ZnO damage from long term exposure to bacterial growth media or to an animal serum, the ZnO layer of the sensor was effectively protected by aluminum oxide (Al2O3) using atomic layer deposition (ALD). As a result, the sensor was reusable for consecutive biofilm formation experiments. The detection limit of the SAW sensor was approximately 5.3 pg. The SAW sensor was tested with Escherichia coli W3110 in Lysogeny Broth (LB) media, and in 10% diluted Fetal Bovine Serum (FBS) as an approximation to an in vivo environment. The resonant frequency shift measured at the output of the SAW sensor in both LB media and 10% FBS corresponded to natural biofilm growth. These repeatable results support the novel application of a SAW sensor for real time biofilm sensing. © 2012 Elsevier B.V. All rights reserved.

Saeed Esmaili Sardari - One of the best experts on this subject based on the ideXlab platform.

  • An ALD aluminum oxide passivated Surface Acoustic Wave sensor for early biofilm detection
    Sensors and Actuators B-chemical, 2012
    Co-Authors: Saeed Esmaili Sardari, A. A. Iliadis, Hsuan Chen Wu, Mariana T. Meyer, William E. Bentley, Reza Ghodssi
    Abstract:

    Abstract We present a successful demonstration of a reusable Surface Acoustic Wave (SAW) sensor for bacterial biofilm growth monitoring in an animal serum and bacterial growth media. Bacterial biofilms produce harmful metabolic by-products and are a characteristic of severe infections. Thus, continuous monitoring of bacterial biofilm growth is critical. Here, we report a highly sensitive SAW sensor for biofilm growth monitoring fabricated by depositing zinc oxide (ZnO) piezoelectric thin film by pulsed laser deposition (PLD). To prevent ZnO damage from long term exposure to bacterial growth media or to an animal serum, the ZnO layer of the sensor was effectively protected by aluminum oxide (Al 2 O 3 ) using atomic layer deposition (ALD). As a result, the sensor was reusable for consecutive biofilm formation experiments. The detection limit of the SAW sensor was approximately 5.3 pg. The SAW sensor was tested with Escherichia coli W3110 in Lysogeny Broth (LB) media, and in 10% diluted Fetal Bovine Serum (FBS) as an approximation to an in vivo environment. The resonant frequency shift measured at the output of the SAW sensor in both LB media and 10% FBS corresponded to natural biofilm growth. These repeatable results support the novel application of a SAW sensor for real time biofilm sensing.

  • An ALD aluminum oxide passivated Surface Acoustic Wave sensor for early biofilm detection
    Sensors and Actuators B: Chemical, 2012
    Co-Authors: Young-wook Kim, Saeed Esmaili Sardari, A. A. Iliadis, Hsuan Chen Wu, Mariana T. Meyer, William E. Bentley, Reza Ghodssi
    Abstract:

    We present a successful demonstration of a reusable Surface Acoustic Wave (SAW) sensor for bacterial biofilm growth monitoring in an animal serum and bacterial growth media. Bacterial biofilms produce harmful metabolic by-products and are a characteristic of severe infections. Thus, continuous monitoring of bacterial biofilm growth is critical. Here, we report a highly sensitive SAW sensor for biofilm growth monitoring fabricated by depositing zinc oxide (ZnO) piezoelectric thin film by pulsed laser deposition (PLD). To prevent ZnO damage from long term exposure to bacterial growth media or to an animal serum, the ZnO layer of the sensor was effectively protected by aluminum oxide (Al2O3) using atomic layer deposition (ALD). As a result, the sensor was reusable for consecutive biofilm formation experiments. The detection limit of the SAW sensor was approximately 5.3 pg. The SAW sensor was tested with Escherichia coli W3110 in Lysogeny Broth (LB) media, and in 10% diluted Fetal Bovine Serum (FBS) as an approximation to an in vivo environment. The resonant frequency shift measured at the output of the SAW sensor in both LB media and 10% FBS corresponded to natural biofilm growth. These repeatable results support the novel application of a SAW sensor for real time biofilm sensing. © 2012 Elsevier B.V. All rights reserved.

A. A. Iliadis - One of the best experts on this subject based on the ideXlab platform.

  • An ALD aluminum oxide passivated Surface Acoustic Wave sensor for early biofilm detection
    Sensors and Actuators B-chemical, 2012
    Co-Authors: Saeed Esmaili Sardari, A. A. Iliadis, Hsuan Chen Wu, Mariana T. Meyer, William E. Bentley, Reza Ghodssi
    Abstract:

    Abstract We present a successful demonstration of a reusable Surface Acoustic Wave (SAW) sensor for bacterial biofilm growth monitoring in an animal serum and bacterial growth media. Bacterial biofilms produce harmful metabolic by-products and are a characteristic of severe infections. Thus, continuous monitoring of bacterial biofilm growth is critical. Here, we report a highly sensitive SAW sensor for biofilm growth monitoring fabricated by depositing zinc oxide (ZnO) piezoelectric thin film by pulsed laser deposition (PLD). To prevent ZnO damage from long term exposure to bacterial growth media or to an animal serum, the ZnO layer of the sensor was effectively protected by aluminum oxide (Al 2 O 3 ) using atomic layer deposition (ALD). As a result, the sensor was reusable for consecutive biofilm formation experiments. The detection limit of the SAW sensor was approximately 5.3 pg. The SAW sensor was tested with Escherichia coli W3110 in Lysogeny Broth (LB) media, and in 10% diluted Fetal Bovine Serum (FBS) as an approximation to an in vivo environment. The resonant frequency shift measured at the output of the SAW sensor in both LB media and 10% FBS corresponded to natural biofilm growth. These repeatable results support the novel application of a SAW sensor for real time biofilm sensing.

  • An ALD aluminum oxide passivated Surface Acoustic Wave sensor for early biofilm detection
    Sensors and Actuators B: Chemical, 2012
    Co-Authors: Young-wook Kim, Saeed Esmaili Sardari, A. A. Iliadis, Hsuan Chen Wu, Mariana T. Meyer, William E. Bentley, Reza Ghodssi
    Abstract:

    We present a successful demonstration of a reusable Surface Acoustic Wave (SAW) sensor for bacterial biofilm growth monitoring in an animal serum and bacterial growth media. Bacterial biofilms produce harmful metabolic by-products and are a characteristic of severe infections. Thus, continuous monitoring of bacterial biofilm growth is critical. Here, we report a highly sensitive SAW sensor for biofilm growth monitoring fabricated by depositing zinc oxide (ZnO) piezoelectric thin film by pulsed laser deposition (PLD). To prevent ZnO damage from long term exposure to bacterial growth media or to an animal serum, the ZnO layer of the sensor was effectively protected by aluminum oxide (Al2O3) using atomic layer deposition (ALD). As a result, the sensor was reusable for consecutive biofilm formation experiments. The detection limit of the SAW sensor was approximately 5.3 pg. The SAW sensor was tested with Escherichia coli W3110 in Lysogeny Broth (LB) media, and in 10% diluted Fetal Bovine Serum (FBS) as an approximation to an in vivo environment. The resonant frequency shift measured at the output of the SAW sensor in both LB media and 10% FBS corresponded to natural biofilm growth. These repeatable results support the novel application of a SAW sensor for real time biofilm sensing. © 2012 Elsevier B.V. All rights reserved.

William E. Bentley - One of the best experts on this subject based on the ideXlab platform.

  • An ALD aluminum oxide passivated Surface Acoustic Wave sensor for early biofilm detection
    Sensors and Actuators B-chemical, 2012
    Co-Authors: Saeed Esmaili Sardari, A. A. Iliadis, Hsuan Chen Wu, Mariana T. Meyer, William E. Bentley, Reza Ghodssi
    Abstract:

    Abstract We present a successful demonstration of a reusable Surface Acoustic Wave (SAW) sensor for bacterial biofilm growth monitoring in an animal serum and bacterial growth media. Bacterial biofilms produce harmful metabolic by-products and are a characteristic of severe infections. Thus, continuous monitoring of bacterial biofilm growth is critical. Here, we report a highly sensitive SAW sensor for biofilm growth monitoring fabricated by depositing zinc oxide (ZnO) piezoelectric thin film by pulsed laser deposition (PLD). To prevent ZnO damage from long term exposure to bacterial growth media or to an animal serum, the ZnO layer of the sensor was effectively protected by aluminum oxide (Al 2 O 3 ) using atomic layer deposition (ALD). As a result, the sensor was reusable for consecutive biofilm formation experiments. The detection limit of the SAW sensor was approximately 5.3 pg. The SAW sensor was tested with Escherichia coli W3110 in Lysogeny Broth (LB) media, and in 10% diluted Fetal Bovine Serum (FBS) as an approximation to an in vivo environment. The resonant frequency shift measured at the output of the SAW sensor in both LB media and 10% FBS corresponded to natural biofilm growth. These repeatable results support the novel application of a SAW sensor for real time biofilm sensing.

  • An ALD aluminum oxide passivated Surface Acoustic Wave sensor for early biofilm detection
    Sensors and Actuators B: Chemical, 2012
    Co-Authors: Young-wook Kim, Saeed Esmaili Sardari, A. A. Iliadis, Hsuan Chen Wu, Mariana T. Meyer, William E. Bentley, Reza Ghodssi
    Abstract:

    We present a successful demonstration of a reusable Surface Acoustic Wave (SAW) sensor for bacterial biofilm growth monitoring in an animal serum and bacterial growth media. Bacterial biofilms produce harmful metabolic by-products and are a characteristic of severe infections. Thus, continuous monitoring of bacterial biofilm growth is critical. Here, we report a highly sensitive SAW sensor for biofilm growth monitoring fabricated by depositing zinc oxide (ZnO) piezoelectric thin film by pulsed laser deposition (PLD). To prevent ZnO damage from long term exposure to bacterial growth media or to an animal serum, the ZnO layer of the sensor was effectively protected by aluminum oxide (Al2O3) using atomic layer deposition (ALD). As a result, the sensor was reusable for consecutive biofilm formation experiments. The detection limit of the SAW sensor was approximately 5.3 pg. The SAW sensor was tested with Escherichia coli W3110 in Lysogeny Broth (LB) media, and in 10% diluted Fetal Bovine Serum (FBS) as an approximation to an in vivo environment. The resonant frequency shift measured at the output of the SAW sensor in both LB media and 10% FBS corresponded to natural biofilm growth. These repeatable results support the novel application of a SAW sensor for real time biofilm sensing. © 2012 Elsevier B.V. All rights reserved.