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Angela M Belcher - One of the best experts on this subject based on the ideXlab platform.

  • real time single walled carbon nanotube based fluorescence imaging improves survival after debulking surgery in an ovarian cancer model
    ACS Nano, 2019
    Co-Authors: Angela M Belcher, Lorenzo Ceppi, Neelkanth M Bardhan, Andrew M Siegel, Nandini Rajan, R Fruscio, Marcela G Del Carmen, Michael J Birrer
    Abstract:

    Improved cytoreductive surgery for advanced stage ovarian cancer (OC) represents a critical challenge in the treatment of the disease. Optimal debulking reaching no evidence of macroscopic disease is the primary surgical end point with a demonstrated survival advantage. Targeted molecule-based fluorescence imaging offers complete tumor resection down to the microscopic scale. We used a custom-built reflectance/fluorescence imaging system with an orthotopic OC mouse model to both quantify tumor detectability and evaluate the effect of fluorescence image-guided surgery on post-operative survival. The contrast agent is an intraperitoneal injectable nanomolecular probe, composed of single-walled carbon nanotubes, coupled to an M13 Bacteriophage carrying a modified peptide binding to the SPARC protein, an extracellular protein overexpressed in OC. The imaging system is capable of detecting a second near-infrared window fluorescence (1000-1700 nm) and can display real-time video imagery to guide intraoperative tumor debulking. We observed high microscopic tumor detection with a pixel-limited resolution of 200 μm. Moreover, in a survival-surgery orthotopic OC mouse model, we demonstrated an increased survival benefit for animals treated with fluorescence image-guided surgical resection compared to standard surgery.

  • Real-Time Single-Walled Carbon Nanotube-Based Fluorescence Imaging Improves Survival after Debulking Surgery in an Ovarian Cancer Model
    2019
    Co-Authors: Lorenzo Ceppi, Angela M Belcher, Neelkanth M Bardhan, Nandini Rajan, R Fruscio, Marcela G Del Carmen, Andrew Siegel, Michael J Birrer
    Abstract:

    Improved cytoreductive surgery for advanced stage ovarian cancer (OC) represents a critical challenge in the treatment of the disease. Optimal debulking reaching no evidence of macroscopic disease is the primary surgical end point with a demonstrated survival advantage. Targeted molecule-based fluorescence imaging offers complete tumor resection down to the microscopic scale. We used a custom-built reflectance/fluorescence imaging system with an orthotopic OC mouse model to both quantify tumor detectability and evaluate the effect of fluorescence image-guided surgery on post-operative survival. The contrast agent is an intraperitoneal injectable nanomolecular probe, composed of single-walled carbon nanotubes, coupled to an M13 Bacteriophage carrying a modified peptide binding to the SPARC protein, an extracellular protein overexpressed in OC. The imaging system is capable of detecting a second near-infrared window fluorescence (1000–1700 nm) and can display real-time video imagery to guide intraoperative tumor debulking. We observed high microscopic tumor detection with a pixel-limited resolution of 200 μm. Moreover, in a survival-surgery orthotopic OC mouse model, we demonstrated an increased survival benefit for animals treated with fluorescence image-guided surgical resection compared to standard surgery

  • versatile de novo enzyme activity in capsid proteins from an engineered M13 Bacteriophage library
    Journal of the American Chemical Society, 2014
    Co-Authors: John P Casey, Roberto J Barbero, Nimrod Heldman, Angela M Belcher
    Abstract:

    Biocatalysis has grown rapidly in recent decades as a solution to the evolving demands of industrial chemical processes. Mounting environmental pressures and shifting supply chains underscore the need for novel chemical activities, while rapid biotechnological progress has greatly increased the utility of enzymatic methods. Enzymes, though capable of high catalytic efficiency and remarkable reaction selectivity, still suffer from relative instability, high costs of scaling, and functional inflexibility. Herein, we developed a biochemical platform for engineering de novo semisynthetic enzymes, functionally modular and widely stable, based on the M13 Bacteriophage. The hydrolytic Bacteriophage described in this paper catalyzes a range of carboxylic esters, is active from 25 to 80 °C, and demonstrates greater efficiency in DMSO than in water. The platform complements biocatalysts with characteristics of heterogeneous catalysis, yielding high-surface area, thermostable biochemical structures readily adaptable...

  • versatile three dimensional virus based template for dye sensitized solar cells with improved electron transport and light harvesting
    PMC, 2013
    Co-Authors: Poyen Chen, Noemiemanuelle Dorval Courchesne, Matthew T Klug, Nicholas X Fang, Xiangnan Dang, Fred J Burpo, Paula T Hammond, Angela M Belcher
    Abstract:

    By genetically encoding affinity for inorganic materials into the capsid proteins of the M13 Bacteriophage, the virus can act as a template for the synthesis of nanomaterial composites for use in various device applications. Herein, the M13 Bacteriophage is employed to build a multifunctional and three-dimensional scaffold capable of improving both electron collection and light harvesting in dye-sensitized solar cells (DSSCs). This has been accomplished by binding gold nanoparticles (AuNPs) to the virus proteins and encapsulating the AuNP-virus complexes in TiO2 to produce a plasmon-enhanced and nanowire (NW)-based photoanode. The NW morphology exhibits an improved electron diffusion length compared to traditional nanoparticle-based DSSCs, and the AuNPs increase the light absorption of the dye-molecules through the phenomenon of localized surface plasmon resonance. Consequently, we report a virus-templated and plasmon-enhanced DSSC with an efficiency of 8.46%, which is achieved through optimizing both the NW morphology and the concentration of AuNPs loaded into the solar cells. In addition, we propose a theoretical model that predicts the experimentally observed trends of plasmon enhancement.

  • viruses as vehicles for growth organization and assembly of materials
    Acta Materialia, 2003
    Co-Authors: Christine E Flynn, Seung-wuk Lee, Beau R Peelle, Angela M Belcher
    Abstract:

    Viruses have been used as scaffolds for the peptide-directed synthesis of magnetic and semiconducting materials, and have been further exploited in the formation of nanowires and liquid crystals. Reviewed in this manuscript is the work of Douglas, Mann, Fraden, Belcher, DeYoreo and others who have either exploited native viral structures to grow or assemble materials, or have genetically modified existing viral structures to specifically affect the growth and mineralization of inorganic materials. Rod-shaped viruses, including M13 Bacteriophage and tobacco mosaic viruses, have been used in the synthesis of nanowires of metals, semiconductors and magnetic materials. The cowpea chlorotic mottle and the cowpea mosaic viruses have been used as nucleation cages for the mineralization of materials such as iron oxide and polyoxometalates. The exterior of such cages has been chemically modified with conjugating linkers as well as with polymeric materials and fluorophores. Further, viral-inorganic complexes have been incorporated into liquid crystal systems as well as self-supporting viral thin films and viral fibers.

Michael J Birrer - One of the best experts on this subject based on the ideXlab platform.

  • real time single walled carbon nanotube based fluorescence imaging improves survival after debulking surgery in an ovarian cancer model
    ACS Nano, 2019
    Co-Authors: Angela M Belcher, Lorenzo Ceppi, Neelkanth M Bardhan, Andrew M Siegel, Nandini Rajan, R Fruscio, Marcela G Del Carmen, Michael J Birrer
    Abstract:

    Improved cytoreductive surgery for advanced stage ovarian cancer (OC) represents a critical challenge in the treatment of the disease. Optimal debulking reaching no evidence of macroscopic disease is the primary surgical end point with a demonstrated survival advantage. Targeted molecule-based fluorescence imaging offers complete tumor resection down to the microscopic scale. We used a custom-built reflectance/fluorescence imaging system with an orthotopic OC mouse model to both quantify tumor detectability and evaluate the effect of fluorescence image-guided surgery on post-operative survival. The contrast agent is an intraperitoneal injectable nanomolecular probe, composed of single-walled carbon nanotubes, coupled to an M13 Bacteriophage carrying a modified peptide binding to the SPARC protein, an extracellular protein overexpressed in OC. The imaging system is capable of detecting a second near-infrared window fluorescence (1000-1700 nm) and can display real-time video imagery to guide intraoperative tumor debulking. We observed high microscopic tumor detection with a pixel-limited resolution of 200 μm. Moreover, in a survival-surgery orthotopic OC mouse model, we demonstrated an increased survival benefit for animals treated with fluorescence image-guided surgical resection compared to standard surgery.

  • Real-Time Single-Walled Carbon Nanotube-Based Fluorescence Imaging Improves Survival after Debulking Surgery in an Ovarian Cancer Model
    2019
    Co-Authors: Lorenzo Ceppi, Angela M Belcher, Neelkanth M Bardhan, Nandini Rajan, R Fruscio, Marcela G Del Carmen, Andrew Siegel, Michael J Birrer
    Abstract:

    Improved cytoreductive surgery for advanced stage ovarian cancer (OC) represents a critical challenge in the treatment of the disease. Optimal debulking reaching no evidence of macroscopic disease is the primary surgical end point with a demonstrated survival advantage. Targeted molecule-based fluorescence imaging offers complete tumor resection down to the microscopic scale. We used a custom-built reflectance/fluorescence imaging system with an orthotopic OC mouse model to both quantify tumor detectability and evaluate the effect of fluorescence image-guided surgery on post-operative survival. The contrast agent is an intraperitoneal injectable nanomolecular probe, composed of single-walled carbon nanotubes, coupled to an M13 Bacteriophage carrying a modified peptide binding to the SPARC protein, an extracellular protein overexpressed in OC. The imaging system is capable of detecting a second near-infrared window fluorescence (1000–1700 nm) and can display real-time video imagery to guide intraoperative tumor debulking. We observed high microscopic tumor detection with a pixel-limited resolution of 200 μm. Moreover, in a survival-surgery orthotopic OC mouse model, we demonstrated an increased survival benefit for animals treated with fluorescence image-guided surgical resection compared to standard surgery

J Pistre - One of the best experts on this subject based on the ideXlab platform.

  • study of acoustic love wave devices for real time Bacteriophage detection
    Sensors and Actuators B-chemical, 2003
    Co-Authors: O Tamarin, Corinne Dejous, Dominique Rebière, J Pistre, S Comeau, Daniel Moynet, Jean Bezian
    Abstract:

    Abstract Acoustic wave devices have shown their good potentialities for real time monitoring of immunoreactions. Different acoustic wave devices (BAW, SHAPM, Love waves) were described for applications in liquid medium. Love wave delay line structures (ST cut quartz substrate with interdigital transducers, SiO2 guiding layer) present several advantages, in particular, the pure shear horizontal polarisation adapted to liquid medium, and its very high sensitivity related to the wave confining in the thin guiding layer. In this paper an analytical method based on multilayer propagating structure is first presented: it allows us to estimate the Love wave phase velocity and then the mass loading effect sensitivity. A few theoretical results are exposed; they show that this theoretical analysis can allow to optimise physical parameters in order to conceive powerful devices for detection applications in liquid medium. As a model for virus or bacteria detection in liquids (drinking or bathing water, food, etc.), we design a model using M13 Bacteriophage. The first step is the anti-M13 antibody binding. By using a Labwindows CVI software, we can monitor in real time the graft of the anti-M13 antibody sensitive coating, as well as the detection of the M13 Bacteriophages. Experimental results are exposed, analysed and discussed. Love waves sensors appear to be a powerful approach for immunodetection, as theoretically predicted.

  • real time device for biosensing design of a Bacteriophage model using love acoustic waves
    Biosensors and Bioelectronics, 2003
    Co-Authors: O Tamarin, Corinne Dejous, Dominique Rebière, S Comeau, Daniel Moynet, Jean Bezian, J Pistre
    Abstract:

    Abstract Love wave sensors (ST-cut quartz substrate with interdigital transducers, SiO 2 guiding layer and sensitive coating) have been receiving a great deal of attention for a few years. Indeed, the wave coupled in a guiding layer confers a high gravimetric sensitivity and the shear horizontal (SH) polarization allows to work in liquid media. In this paper, an analytical method is proposed to calculate the Love wave phase velocity and the gravimetric sensitivity for a complete multilayer structure. This allows us to optimize the Love wave devices design in order to improve their gravimetric sensitivity in liquid media. As a model for virus or bacteria detection in liquids (drinking or bathing water, food…) we design a model using M13 Bacteriophage. The first step is the anti-M13 (AM13) monoclonal antibody grafting, on the device surface (SiO 2 ). The second step is an immunoreaction in between the M13 Bacteriophage and the AM13 antibody. The Love wave device allows to detect in real time the graft of the AM13 sensitive coating, as well as the immobilization of the M13 Bacteriophages. With a pH change, the M13 Bacteriophages can be removed from the sensor surface, in order to be numerated as plaque forming unit (pfu). Results on the sensitivity of Love waves are compared with similar immunological works with bulk acoustic wave devices, and demonstrate the high potentialities of Love waves sensors.

Jean Bezian - One of the best experts on this subject based on the ideXlab platform.

  • study of acoustic love wave devices for real time Bacteriophage detection
    Sensors and Actuators B-chemical, 2003
    Co-Authors: O Tamarin, Corinne Dejous, Dominique Rebière, J Pistre, S Comeau, Daniel Moynet, Jean Bezian
    Abstract:

    Abstract Acoustic wave devices have shown their good potentialities for real time monitoring of immunoreactions. Different acoustic wave devices (BAW, SHAPM, Love waves) were described for applications in liquid medium. Love wave delay line structures (ST cut quartz substrate with interdigital transducers, SiO2 guiding layer) present several advantages, in particular, the pure shear horizontal polarisation adapted to liquid medium, and its very high sensitivity related to the wave confining in the thin guiding layer. In this paper an analytical method based on multilayer propagating structure is first presented: it allows us to estimate the Love wave phase velocity and then the mass loading effect sensitivity. A few theoretical results are exposed; they show that this theoretical analysis can allow to optimise physical parameters in order to conceive powerful devices for detection applications in liquid medium. As a model for virus or bacteria detection in liquids (drinking or bathing water, food, etc.), we design a model using M13 Bacteriophage. The first step is the anti-M13 antibody binding. By using a Labwindows CVI software, we can monitor in real time the graft of the anti-M13 antibody sensitive coating, as well as the detection of the M13 Bacteriophages. Experimental results are exposed, analysed and discussed. Love waves sensors appear to be a powerful approach for immunodetection, as theoretically predicted.

  • real time device for biosensing design of a Bacteriophage model using love acoustic waves
    Biosensors and Bioelectronics, 2003
    Co-Authors: O Tamarin, Corinne Dejous, Dominique Rebière, S Comeau, Daniel Moynet, Jean Bezian, J Pistre
    Abstract:

    Abstract Love wave sensors (ST-cut quartz substrate with interdigital transducers, SiO 2 guiding layer and sensitive coating) have been receiving a great deal of attention for a few years. Indeed, the wave coupled in a guiding layer confers a high gravimetric sensitivity and the shear horizontal (SH) polarization allows to work in liquid media. In this paper, an analytical method is proposed to calculate the Love wave phase velocity and the gravimetric sensitivity for a complete multilayer structure. This allows us to optimize the Love wave devices design in order to improve their gravimetric sensitivity in liquid media. As a model for virus or bacteria detection in liquids (drinking or bathing water, food…) we design a model using M13 Bacteriophage. The first step is the anti-M13 (AM13) monoclonal antibody grafting, on the device surface (SiO 2 ). The second step is an immunoreaction in between the M13 Bacteriophage and the AM13 antibody. The Love wave device allows to detect in real time the graft of the AM13 sensitive coating, as well as the immobilization of the M13 Bacteriophages. With a pH change, the M13 Bacteriophages can be removed from the sensor surface, in order to be numerated as plaque forming unit (pfu). Results on the sensitivity of Love waves are compared with similar immunological works with bulk acoustic wave devices, and demonstrate the high potentialities of Love waves sensors.

Daniel Moynet - One of the best experts on this subject based on the ideXlab platform.

  • Detection of Bacteriophages in dynamic mode using a Love-wave immunosensor with microfluidics technology
    Sensors and Actuators B: Chemical, 2013
    Co-Authors: D. Matatagui, Corinne Dejous, Dominique Rebière, Daniel Moynet, M.j. Fernández, J. Fontecha, J.p. Esquivel, I. Gràcia, C. Cané, J.p. Santos
    Abstract:

    An immunosensor based on a Love-wave device and a PDMS microfluidic chip has been developed to detect potentially pathogenic microorganisms in real time. In order to characterize and test the immunosensor, the M13 Bacteriophage has been chosen as a model of microorganism. The M13 was detected with the mouse monoclonal antibody, anti-M13 (AM13), which has been immobilized on the Love wave-device. The immunoassay has been carried out in a dynamic mode (continuous flow) through microchannels with the goal of obtaining a short response time in detecting a small volume of a sample of M13 Bacteriophage. The concentrations of the Bacteriophage have been varied between 5 × 10^8 pfu ml^−1 and 2 × 10^10 pfu ml^−1, a range for which a fast detection and a favorable discrimination among concentrations have been achieved. The AM13 has been also used as a secondary antibody in order to measure lower concentrations such as 5 × 10^7 pfu ml^−1.The Double-Layer Agar technique has been used to quantify, as plaques-forming unit (pfu), the number of M13 Bacteriophage in the initial sample as well as those linked to the sensor surface during the detection. Finally, the number of Bacteriophage was estimated by calculating the mass sensitivity. The values obtained in both procedures have been very similar.

  • study of acoustic love wave devices for real time Bacteriophage detection
    Sensors and Actuators B-chemical, 2003
    Co-Authors: O Tamarin, Corinne Dejous, Dominique Rebière, J Pistre, S Comeau, Daniel Moynet, Jean Bezian
    Abstract:

    Abstract Acoustic wave devices have shown their good potentialities for real time monitoring of immunoreactions. Different acoustic wave devices (BAW, SHAPM, Love waves) were described for applications in liquid medium. Love wave delay line structures (ST cut quartz substrate with interdigital transducers, SiO2 guiding layer) present several advantages, in particular, the pure shear horizontal polarisation adapted to liquid medium, and its very high sensitivity related to the wave confining in the thin guiding layer. In this paper an analytical method based on multilayer propagating structure is first presented: it allows us to estimate the Love wave phase velocity and then the mass loading effect sensitivity. A few theoretical results are exposed; they show that this theoretical analysis can allow to optimise physical parameters in order to conceive powerful devices for detection applications in liquid medium. As a model for virus or bacteria detection in liquids (drinking or bathing water, food, etc.), we design a model using M13 Bacteriophage. The first step is the anti-M13 antibody binding. By using a Labwindows CVI software, we can monitor in real time the graft of the anti-M13 antibody sensitive coating, as well as the detection of the M13 Bacteriophages. Experimental results are exposed, analysed and discussed. Love waves sensors appear to be a powerful approach for immunodetection, as theoretically predicted.

  • real time device for biosensing design of a Bacteriophage model using love acoustic waves
    Biosensors and Bioelectronics, 2003
    Co-Authors: O Tamarin, Corinne Dejous, Dominique Rebière, S Comeau, Daniel Moynet, Jean Bezian, J Pistre
    Abstract:

    Abstract Love wave sensors (ST-cut quartz substrate with interdigital transducers, SiO 2 guiding layer and sensitive coating) have been receiving a great deal of attention for a few years. Indeed, the wave coupled in a guiding layer confers a high gravimetric sensitivity and the shear horizontal (SH) polarization allows to work in liquid media. In this paper, an analytical method is proposed to calculate the Love wave phase velocity and the gravimetric sensitivity for a complete multilayer structure. This allows us to optimize the Love wave devices design in order to improve their gravimetric sensitivity in liquid media. As a model for virus or bacteria detection in liquids (drinking or bathing water, food…) we design a model using M13 Bacteriophage. The first step is the anti-M13 (AM13) monoclonal antibody grafting, on the device surface (SiO 2 ). The second step is an immunoreaction in between the M13 Bacteriophage and the AM13 antibody. The Love wave device allows to detect in real time the graft of the AM13 sensitive coating, as well as the immobilization of the M13 Bacteriophages. With a pH change, the M13 Bacteriophages can be removed from the sensor surface, in order to be numerated as plaque forming unit (pfu). Results on the sensitivity of Love waves are compared with similar immunological works with bulk acoustic wave devices, and demonstrate the high potentialities of Love waves sensors.