The Experts below are selected from a list of 147 Experts worldwide ranked by ideXlab platform
C Juan - One of the best experts on this subject based on the ideXlab platform.
-
development and validation of a liquid chromatography tandem mass spectrometry method for the analysis of β agonists in animal feed and drinking water
Journal of Chromatography A, 2010Co-Authors: Francisco Moragues, Carmen Igualada, C Juan, Nuria Leon, J ManesAbstract:Abstract A reproducible, sensitive and selective multiresidue analytical method for seven β-agonists: clenbuterol (CBT), clenpenterol (CPT), ractopamine (RTP), brombuterol (BBT), Mabuterol (MBT), mapenterol (MPT), and hydroxymethylclenbuterol (HMCBT) was developed and validated by using liquid chromatography tandem mass spectrometry (LC–MS/MS) in feed and drinking water samples. The validation was achieved according to the criteria laid down in the Commission Decision 2002/657/EC, however it was necessary to use minimum required performance limits (MRPLs) proposed by the Community Reference Laboratories (CRLs) due to the lack of maximum residue limits (MRLs) for β-agonists. By setting up these MRPLs, allows controlling their use in safe mode, since β-agonists are commonly used in veterinary medicine sometime in a fraudulent manner, for increasing the weigh of animals. Values set for both matrices studied are 50 μg/kg for animal feed, and a range from 0.2 to 10 μg/L for drinking water. CCα values calculated were under the MRPLs suggested; for drinking water the lowest value obtained was 0.12 μg/L, and for animal feed 0.87 μg/kg. Values for CCβ were ranged from 0.08 to 0.13 μg/L in drinking water and from 0.5 to 0.92 μg/kg in animal feed samples. The excellence values obtained, allowed us to conclude that the proposed analytical method is capable to control the β-agonists studied in both matrices and that it can be successfully applied and used as a routine method in laboratories of residue analysis of veterinary food control.
Shen Baochun - One of the best experts on this subject based on the ideXlab platform.
-
enantioseparation of clenbuterol and Mabuterol on self made 3 4 dichlorovancomycin chiral stationary phase
Journal of Kunming Medical University, 2013Co-Authors: Shen BaochunAbstract:Objective Using the polar mobile phase,the enantioseparation of clenbuterol and Mabuterol on the self-made 3,4-dichlorovancomycin chiral stationary phase were studied.Methods The mobile phase was methanol,adding different proportions of acetic acid and triethylamine.The influence of different proportions of acid and alkali additives was investigated.The effects of column temperature and flow rate of mobile phase on the enantioseparation were also studied.Results With a mobile phase consisting of methanol : aceticacid : triethylamine = 100 : 0.05 : 0.05(V:V:V) at a flow rate of 1.0 mL/min,the separation factor for clenbuterol and Mabuterol were 1.232 and 1.299,and the resolution were 1.006 and 1.015,respectively.Conclusion The results indicated that the isomers of clenbuterol and Mabuterol could be separated on self-made 3,4-dichlorovancomycin chiral stationary phase.
-
enantioseparation of clenbuterol and Mabuterol on the teicoplanin chiral stationary phase
Journal of Kunming Medical University, 2013Co-Authors: Shen BaochunAbstract:Objective Using the polar mobile phase,the enantioseparation of clenbuterol and Mabuterol on the self-made teicoplanin chairal stationary phase were studied.Methods The mobile phase consisted of methanol : ethanol:acetic acid:triethylamine = 50:50:0.05:0.05(V/V/V)with a flow rate of 1.0 mL/min and column temperature of 25 ℃.The influences of the proportion of acetic acid and triethyamine,ethanol concentration,column temperature and flow rate on the enantioseparation were investigated.The chiral recognition mechanism was also discussed.Results Under the optimal conditions,the separation factor for clenbuterol and Mabuterol was 1.37 and 1.51,respectively.Conclusion The result showed that the isomers of clenbuterol and Mabuterol could be separated on the self-made teicoplanin chairal stationary phase in the polar mobile phase.
J Manes - One of the best experts on this subject based on the ideXlab platform.
-
development and validation of a liquid chromatography tandem mass spectrometry method for the analysis of β agonists in animal feed and drinking water
Journal of Chromatography A, 2010Co-Authors: Francisco Moragues, Carmen Igualada, C Juan, Nuria Leon, J ManesAbstract:Abstract A reproducible, sensitive and selective multiresidue analytical method for seven β-agonists: clenbuterol (CBT), clenpenterol (CPT), ractopamine (RTP), brombuterol (BBT), Mabuterol (MBT), mapenterol (MPT), and hydroxymethylclenbuterol (HMCBT) was developed and validated by using liquid chromatography tandem mass spectrometry (LC–MS/MS) in feed and drinking water samples. The validation was achieved according to the criteria laid down in the Commission Decision 2002/657/EC, however it was necessary to use minimum required performance limits (MRPLs) proposed by the Community Reference Laboratories (CRLs) due to the lack of maximum residue limits (MRLs) for β-agonists. By setting up these MRPLs, allows controlling their use in safe mode, since β-agonists are commonly used in veterinary medicine sometime in a fraudulent manner, for increasing the weigh of animals. Values set for both matrices studied are 50 μg/kg for animal feed, and a range from 0.2 to 10 μg/L for drinking water. CCα values calculated were under the MRPLs suggested; for drinking water the lowest value obtained was 0.12 μg/L, and for animal feed 0.87 μg/kg. Values for CCβ were ranged from 0.08 to 0.13 μg/L in drinking water and from 0.5 to 0.92 μg/kg in animal feed samples. The excellence values obtained, allowed us to conclude that the proposed analytical method is capable to control the β-agonists studied in both matrices and that it can be successfully applied and used as a routine method in laboratories of residue analysis of veterinary food control.
Carmen Igualada - One of the best experts on this subject based on the ideXlab platform.
-
development and validation of a liquid chromatography tandem mass spectrometry method for the analysis of β agonists in animal feed and drinking water
Journal of Chromatography A, 2010Co-Authors: Francisco Moragues, Carmen Igualada, C Juan, Nuria Leon, J ManesAbstract:Abstract A reproducible, sensitive and selective multiresidue analytical method for seven β-agonists: clenbuterol (CBT), clenpenterol (CPT), ractopamine (RTP), brombuterol (BBT), Mabuterol (MBT), mapenterol (MPT), and hydroxymethylclenbuterol (HMCBT) was developed and validated by using liquid chromatography tandem mass spectrometry (LC–MS/MS) in feed and drinking water samples. The validation was achieved according to the criteria laid down in the Commission Decision 2002/657/EC, however it was necessary to use minimum required performance limits (MRPLs) proposed by the Community Reference Laboratories (CRLs) due to the lack of maximum residue limits (MRLs) for β-agonists. By setting up these MRPLs, allows controlling their use in safe mode, since β-agonists are commonly used in veterinary medicine sometime in a fraudulent manner, for increasing the weigh of animals. Values set for both matrices studied are 50 μg/kg for animal feed, and a range from 0.2 to 10 μg/L for drinking water. CCα values calculated were under the MRPLs suggested; for drinking water the lowest value obtained was 0.12 μg/L, and for animal feed 0.87 μg/kg. Values for CCβ were ranged from 0.08 to 0.13 μg/L in drinking water and from 0.5 to 0.92 μg/kg in animal feed samples. The excellence values obtained, allowed us to conclude that the proposed analytical method is capable to control the β-agonists studied in both matrices and that it can be successfully applied and used as a routine method in laboratories of residue analysis of veterinary food control.
-
how to decrease ion suppression in a multiresidue determination of β agonists in animal liver and urine by liquid chromatography mass spectrometry with ion trap detector
Analytica Chimica Acta, 2009Co-Authors: Francisco Moragues, Carmen IgualadaAbstract:A new liquid chromatography-mass spectrometry with ion-trap detection method for the determination of clenbuterol, ractopamine, clenpenterol, brombuterol, hydroxyclenbuterol, mapenterol and Mabuterol in animal liver and urine is described to decrease ion suppression in both matrices. The developed method consists of an aqueous extraction and a two-step clean-up. Final extract was injected in a liquid chromatograph with ion trap mass spectrometer optimized to obtain MS(3) ions which provided more specificity and better signal to noise ratio in the investigated analytes. Those steps have been essential to reach decision limits (CCalpha) values below the proposed MRPL values for each substance. The CCalpha were determined and their values were 0.05 microgkg(-1) for clenbuterol, 0.2 microgkg(-1) for ractopamine and 0.1 microgkg(-1) for the other analytes in liver and the same values in microgL(-1) for urine samples.
Zonghui Yuan - One of the best experts on this subject based on the ideXlab platform.
-
development of monoclonal antibodies and indirect competitive enzyme linked immunosorbent assay kits for the detection of clenbuterol and salbutamol in the tissues and products of food producing animals
Food Analytical Methods, 2017Co-Authors: Dapeng Peng, Lingyan Zhang, Chen Situ, Yuanhu Pan, Yanfei Tao, Yulian Wang, Zonghui YuanAbstract:To better monitor the residues of clenbuterol and salbutamol in edible tissues and products of animals treated with these compounds, a monoclonal antibody (mAb) against the β-agonists was prepared, and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on this antibody was also developed. The hapten of salbutamol was synthesized and conjugated to carrier proteins via different linkers. Female Balb/c mice were immunized with the hapten-protein conjugates to produce monoclonal antibodies using the standard fusion procedures. After fusion and four cloning cycles, eight hybridomas were isolated and of which one clone, 6E5, that has the isotype IgG1, was selected for the present study. The cross-reactivities of the mAb 6E5 towards salbutamol, clenbuterol, Mabuterol, cimaterol, clenproperol, mapenterol, tuoloterol and terbutaline were determined as 104.5, 100, 100, 83.6, 71.9, 44.2, 6.3 and 2.3%, respectively. The limit of detection for salbutamol and clenbuterol in edible animal tissues was 0.21 and 0.57 μg kg−1, respectively. The recoveries ranged from 55.4 to 122.0%, and the CVs were less than 19.6%. When used with spiked or incurred samples, or in a field trial, the established ic-ELISA has demonstrated a consistent performance in various biological matrices, suggesting this is a sensitive, accurate and low-cost analytical method.