Maclura

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G. Delle Monache - One of the best experts on this subject based on the ideXlab platform.

  • Maclura pomifera (Osage Orange): In Vitro Culture and the Formation of Flavonoids and Other Secondary Metabolites
    Medicinal and Aromatic Plants VIII, 1995
    Co-Authors: B. Botta, G. Delle Monache
    Abstract:

    Maclura pomifera (Raf.) Schneid. (syn Maclura aurantiaca or Toxylon pomiferum) is the only species of the genus Maclura, belonging to the family Moraceae. The plant is a hardwood tree native to the southwestern United States (Texas and Arkansas), but dispersed also in Europe (Fig. 1A). The tree, which may attain the height of 20 m, has a trunk covered by a furrowed orangecolored bark and thorny branches, surmounted by a long, luxuriant, dishevelled hair.

  • Comparison between metabolite productions in cell culture and in whole plant of Maclura pomifera.
    Phytochemistry, 1995
    Co-Authors: G. Delle Monache, Gabriella Pasqua, Barbara Monacelli, A. Cuteri, Alberto Vitali, R. Scurria, Maria Cristina De Rosa, Bruno Botta
    Abstract:

    Plant tissue cultures of Maclura pomifera showed a metabolite accumulation pattern which was both quantitatively and qualitatively different from that of the parent plant. Triterpenes and flavonoids were isolated from callus and cell cultures, however, xanthones and stilbenes, which have been reported in the whole plant, were not found. Among the flavonoids, flavones and flavanones were produced preferentially by the suspended cells, but with the prenyl substituents exclusively on ring A, while the isoflavones did not show the 3',4'-dihydroxyl substitution pattern found in the products isolated from fruits. A new prenylated 6'-deoxychalcone was also isolated from the callus and cell cultures.

  • Two isoflavones and a flavone from the fruits of Maclura pomifera
    Phytochemistry, 1994
    Co-Authors: G. Delle Monache, Gabriella Pasqua, Barbara Monacelli, Bruno Botta, Alberto Vitali, R. Scurria, Cleofe Palocci, Enrico Cernia
    Abstract:

    Abstract Stabilized and optimized cell suspension cultures of Maclura pomifera showed a flavonoid accumulation qualitatively different from that of the intact plant. For a better comparison between in vivo and in vitro production, a re-examination of the fruit extract was undertaken. The study, involving super fluid chromatography, led to the isolation and structure determination of flavonoids not previously reported in the plant. Three of these compounds are new. The presence of flavonoids in stems and leaves was also investigated.

  • Cell Suspension Cultures of Maclura pomifera: Optimization of Growth and Metabolite Production
    Journal of Plant Physiology, 1991
    Co-Authors: Gabriella Pasqua, Barbara Monacelli, A. Cuteri, O. Finocchiaro, Bruno Botta, Alberto Vitali, G. Delle Monache
    Abstract:

    Summary Explants of stem of Maclura pomifera were cultured in media with varied concentrations of hormones. The obtained calli were separated on the basis of colour into two lines, A and B, which gave different metabolite accumulations. The major components of the extracts were A-ring prenylated flavanones, isoflavones and flavones. Cell suspension cultures of lines A and B showed the same difference together with a considerable increase in flavonoid production.

James L. Hamrick - One of the best experts on this subject based on the ideXlab platform.

Vitenko V.a. - One of the best experts on this subject based on the ideXlab platform.

Ratko M. Jankov - One of the best experts on this subject based on the ideXlab platform.

  • Isolation and characterization of Maclura (Maclura pomifera) extracts obtained by supercritical fluid extraction
    Industrial Crops and Products, 2015
    Co-Authors: Snezana Filip, Zoltan Djarmati, Kiril Lisichkov, János Csanádi, Ratko M. Jankov
    Abstract:

    Abstract The chemical composition of the CO 2 extract of Maclura fruit (Osage orange) was analyzed. The major constituents of the CO 2 extracts were triterpene alcohol esters, along with the triglycerides of C 16 and C 18 fatty acids. Column chromatography of the CO 2 extract yielded lupeol ester of hexadecanoic acid ( 2 ), lupeol ester of 3-hydroxyhexadecanoic acid (β-hydroxy palmitic acid) ( 3 ) trace of lupeol ( 1 ), butyrospermol ester of hexadecanoic acid ( 5 ), butyrospermol acetate ( 6 ) and trace of butyrospermol ( 4 ) as the least polar components. By consequent extraction with acetone of the lipid free Maclura material an extract was obtained, in which isoflavonoids osajin ( 7 ) and pomiferin ( 8 ) (natural antioxidant) were found in high yields. The structure of isolated compounds was established by spectroscopic data (IR, 1 H and 13 C NMR and MS) and some chemical reactions. The 3 is new natural product, not yet described in literature.

Willy J. Peumans - One of the best experts on this subject based on the ideXlab platform.

  • Molecular cloning of the mitogenic mannose/maltose-specific rhizome lectin from Calystegia sepium.
    FEBS Letters, 1996
    Co-Authors: E J Van Damme, A Barre, P Verhaert, P Rougé, Willy J. Peumans
    Abstract:

    cDNA clones encoding the mitogenic mannose/maltose-specific lectin from the rhizomes of hedge bindweed (Calystegia sepium) have been isolated and sequenced. Comparison of the deduced amino acid sequence and the molecular weight of the lectin subunit as determined by mass spectrometry indicated that the mature protein comprises the entire open reading frame of the cDNA, which implies that the primary translation product contains no signal peptide and is not proteolytically processed. Searches in the databases revealed sequence homology with the previously described lectins from the taxonomically unrelated Moraceae species Artocarpus integrifolia and Maclura pomifera.

  • molecular cloning of the mitogenic mannose maltose specific rhizome lectin from calystegia sepium
    FEBS Letters, 1996
    Co-Authors: E J Van Damme, A Barre, P Verhaert, P Rougé, Willy J. Peumans
    Abstract:

    cDNA clones encoding the mitogenic mannose/mal-tose-specific lectin from the rhizomes of hedge bindweed (Calystegia sepium) have been isolated and sequenced. Comparison of the deduced amino acid sequence and the molecular weight of the lectin subunit as determined by mass spectrometry indicated that the mature protein comprises the entire open reading frame of the cDNA, which implies that the primary translation product contains no signal peptide and is not proteolytically processed. Searches in the databases revealed sequence homology with the previously described lectins from the taxonomically unrelated Moraceae species Artocarpus integrifolia and Maclura pomifera.