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E P Kozlovskaya - One of the best experts on this subject based on the ideXlab platform.
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study of the interaction of trypsin inhibitor from the sea anemone radianthus Macrodactylus with proteases
Biochemistry (moscow) Supplement Series B: Biomedical Chemistry, 2007Co-Authors: I N Sokotun, O V Gnedenko, A A Molnar, M M Monastyrnaya, E P Kozlovskaya, A V Leychenko, A S IvanovAbstract:The interaction of the inhibitor VJ (InhVJ), isolated from sea anemone R. Macrodactylus, with different proteases was investigated using the method of biosensor analysis. The following enzymes were tested: serine proteases (trypsin, α-chymotrypsin, plasmin, thrombin, kallikrein), cysteina protease (papain) and aspartic protease (pepsin). In the rage of the concentrations studied (10–400 nM) inhibitor VJ interacted only with trypsin and α-chymotrypsin. The intermolecular complexes formation between inhibitor VJ and each of these enzymes was characterized by the following kinetic and thermodynamics parameters: KD = 7.38 × 10−8 M and 9.93 × 10−7 M for pairs InhVJ/trypsin and InhVJ/α-chymotrypsin, respectively.
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proteinase inhibitors from the tropical sea anemone radianthus Macrodactylus isolation and characteristic
Biochemistry, 2007Co-Authors: I N Sokotun, Elena Leychenko, M M Monastyrnaya, A P Ilina, A A Eskov, S D Anastuk, E P KozlovskayaAbstract:Two new serine proteinase inhibitors (RmIn I and RmIn II) from the tropical sea anemone Radianthus Macrodactylus have been isolated and characterized. The purification procedure includes polychrome-1 hydrophobic chromatography, Superdex™ Peptide 10/30 FPLC, and Nucleosil C18 reverse-phase HPLC. The molecular masses of RmIn I, RmIn II, and the complexes RmIn II/trypsin and RmIn I,II/α-chymotrypsin have been determined. The K i values of RmIn I and RmIn II for trypsin and α-chymotrypsin have been determined. The polypeptides RmIn I and RmIn II are shown to be nontoxic and to exhibit antihistamine activity. The N-terminal amino acid sequences of RmIn I (GICSEPIVVGPCKAG-) and RmIn II (GSTCLEPKVVGPCKA-) have been determined. A high homology of the amino acid sequences is demonstrated for the proteinase inhibitors produced by such evolutionarily distant species as coelenterates, reptiles, and mammals.
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amino acid sequence of rtx a s isoform actinoporin from the sea anemone radianthus Macrodactylus
Toxicon, 2006Co-Authors: Anna Ilina, A V Lipkin, Ekaterina V Barsova, Marina P Issaeva, Elena Leychenko, Konstantin V Guzev, M M Monastyrnaya, Sergey Lukyanov, E P KozlovskayaAbstract:The amino acid sequence of actinoporin RTX-A (175 aa) from the sea anemone Radianthus Macrodactylus was determined by sequencing of clones obtained via amplification of cDNA. It was established that RTX-A possessed high homology with HmgIII from Heteractis magnifica (87%) and StI, StII from Stichodactyla helianthus (84 and 87%, respectively). The analysis of structural and functional relationships within RTX-A was carried out. The some disagreement concerning to significant role of several amino acid residues for actinoporins exhibition of hemolytic activity was found.
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biologically active polypeptides from the tropical sea anemone radianthus Macrodactylus
Toxicon, 2002Co-Authors: M M Monastyrnaya, Tatyana A Zykova, Olga V Apalikova, Tatyana V Shwets, E P KozlovskayaAbstract:Abstract Some biologically active polypeptides, three high and two low molecular weight cytolysins and four trypsin inhibitors were isolated from the sea anemone Radianthus Macrodactylus and characterized. The purification steps involved acetone precipitation, gel filtration, ion-exchange, and affinity chromatography, and ion-exchange and reverse-phase HPLC. The relative molecular weight of high molecular weight Radianthus cytolysins named according to their N-terminal amino acids RTX-A (Ala), RTX-S (Ser) and RTX-G (Gly) was about 20,000. The isoelectric points were 9.8 for RTX-A and RTX-S, and 10.5 for RTX-G. The hemolytic activities of RTX-A, RTX-S and RTX-G were 3.5×104, 5.0×104, and 1.0×104 HU/mg, respectively, and were inhibited by sphingomyelin. The N-terminal amino acid sequence of RTX-A was determined as ALAGAIIAGAGLGLKILIEVLGEG–VKVKI–. Molecular weight of low molecular weight Radianthus cytolysins RmI, RmII, and of one trypsin inhibitor InI were 5100, 6100 and 7100, respectively. Isoelectric points for RmI and RmII were 9.2 and 9.3. Their hemolytic activity worked out 25 and 20 HU/mg, and was not inhibited by sphingomyelin. Toxicity of RmI and RmII was assessed by their histaminolytic activity. Amino acid composition of RmI and RmII was similar to that of tealiatoxin, histaminolytic cytolysin from the sea anemone Tealia felina.
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sea anemone radianthus Macrodactylus a new source of palytoxin
Toxicon, 1992Co-Authors: Vladimir M Mahnir, E P Kozlovskaya, Anatoly I KalinovskyAbstract:A very potent non-protein toxin was isolated from the sea anemone Radianthus Macrodactylus with the use of chromatography on polytetrafluoroethylene, CM-Sephadex C-25 and by cation and anion exchange HPLC. The toxin was identified as palytoxin by u.v.-, i.r.- and 500 MHz 1H NMR spectroscopy. Its LD50 was 0.74 +/- 0.29 micrograms/kg by i.v. injection into mice. So far, palytoxin has been associated with zoanthids only. The toxin caused the loss of haemoglobin from erythrocytes but only in about 2 hr after the beginning of incubation, which is characteristic for palytoxin from zoanthids. Sea anemone palytoxin was divided into major and minor components by HPLC. The latter proved to be a product of degradation of palytoxin.
Miguel Ángel Morón - One of the best experts on this subject based on the ideXlab platform.
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review of the species of Macrodactylus dejean coleoptera scarabaeidae melolonthinae from the central american nucleus
Zootaxa, 2020Co-Authors: Roberto Arceperez, Miguel Ángel MorónAbstract:Twenty species of Macrodactylus Dejean (Coleoptera: Scarabaeidae: Melolonthinae: Macrodactylini) from the Central American nucleus are described and illustrated, including two new species from Guatemala: Macrodactylus eniocanoi new species and Macrodactylus escuintlaensis new species. Macrodactylus hondurensis Arce-Perez Moron is recorded for the first time from El Salvador. Diagnoses for 18 species previously recorded in the Central American nucleus are provided. A key to all known species of Macrodactylus of Central American nucleus and distributional maps are included.
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review of the species of Macrodactylus dejean coleoptera scarabaeidae melolonthinae from costa rica and panama
Zootaxa, 2018Co-Authors: Roberto Arceperez, Miguel Ángel MorónAbstract:Fifteen species of Macrodactylus Dejean (Coleoptera: Scarabaeidae: Melolonthinae: Macrodactylini) from Costa Rica and Panama are described and illustrated, including eight new species: M. latreillei new species., M. pseudothoracicus new species. and M. dejeani new species from Costa Rica; M. solisi new species from Costa Rica and Panama; M. martinezi new species from Costa Rica and Venezuela; M. chiriquiensis new species and M. kunayalaensis new species from Panama; and M. moroni new species from Costa Rica, and Panama. Diagnosis for seven species previously recorded in Costa Rica and Panama are included, and a key to all known species of Macrodactylus in these countries is provided.
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new species and records of Macrodactylus dejean coleoptera scarabaeidae melolonthinae macrodactylini from bolivia
Zootaxa, 2014Co-Authors: Roberto Arceperez, Miguel Ángel MorónAbstract:Two new species of Macrodactylus Dejean (Coleoptera: Scarabaeidae: Melolonthinae) from Bolivia are described and illustrated: M. megaphyllus new species (from Comarapa, Santa Cruz and Sehuenca, Cochabamba) and M. yunganus new species (from Mairana and Comarapa, Santa Cruz). In addition, the species Macrodactylus bolivianus Moser, M. gracilis Moser, and M. nobilis Frey are redescribed and illustrated to help facilitate identification of these species. A key to the 10 species of Macrodactylus presently known from Bolivia is provided.
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Description of the Larvae of Three Species of Macrodactylus Dejean (Coleoptera: Scarabaeidae: Melolonthinae) from Mexico, with Notes on the Reproductive Behavior of Macrodactylus ocreatus Bates
The Coleopterists Bulletin, 2010Co-Authors: Agustín Aragón-garcía, Miguel Ángel Morón, Sara Yunuén Rodríguez-velázquez, Amada Nayeli Cortés-meza, Mariana Zarazúa-carvajal, Miguel Ángel Damián-huatoAbstract:ABSTRACT Third instars of Macrodactylus mexicanus Burmeister, Macrodactylus nigripes Bates, and Macrodactylus ocreatus Bates are described from specimens collected from soil in corn fields at Chignahuapan, Puebla and La Malinche, Tlaxcala, and from soil in an amaranth grain field at Zapotitlan Salinas, Puebla. Illustrations of diagnostic structures, comparative comments with similar species, and a key to known third instars of Macrodactylus Dejean in Mexico are included. Data on the habits and reproductive behavior of M. ocreatus in nature and in captivity are also provided.
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new species of Macrodactylus dejean coleoptera scarabaeidae melolonthinae macrodactylini from mexico
Coleopterists Bulletin, 2009Co-Authors: Roberto Arceperez, Miguel Ángel MorónAbstract:Abstract Macrodactylus howdeni, new species, from pine-oak forest at 2,600 m elevation near Toluca, state of Mexico, and Macrodactylus noveloi, new species, from cloud forest at 1,050 m elevation in the state of Michoacan and oak forests at 780–1,160 m elevation in the state of Nayarit, Mexico are described. Illustrations of diagnostic characters and comparative comments with similar species are included. New records are provided for Macrodactylus submarginatus Bates for the state of Michoacan, Macrodactylus pokornyanus Arce-Perez and Moron for the state of Mexico, and Macrodactylus longicollis (Latreille) for the state of Oaxaca. With these additions, 27 species of Macrodactylus Dejean are recorded from Mexico.
Roberto Arceperez - One of the best experts on this subject based on the ideXlab platform.
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actualizacion de las especies del genero Macrodactylus dejean coleoptera melolonthinae macrodactylini para el estado de puebla mexico
ACTA ZOOLÓGICA MEXICANA (N.S.), 2021Co-Authors: Miguel Aragonsanchez, Roberto Arceperez, Agustin Aragon Garcia, Betzabeth Cecilia Perez Torres, Victor Alfonzo Cuatemozo, Jesus Francisco LopezolguinAbstract:El estado de Puebla, Mexico, ocupa el quinto lugar en riqueza de especies del genero Macrodactylus con nueve especies. Por medio de la revision de colecciones cientificas y trabajo de campo, se actualizo el numero de especies a diez, con Macrodactylus lineatocollis Bates 1887, como nuevo registro estatal. Se presentan mapas con su distribucion territorial, que se relacionan con las variables de temperatura media anual y rangos de humedad. Se hacen comentarios biologicos para cada especie y se presenta una clave dicotomica para la identificacion de las especies presentes en el estado de Puebla.
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two new species of Macrodactylus coleoptera scarabaeidae melolonthinae macrodactylini from mexico and guatemala
Zootaxa, 2021Co-Authors: Roberto Arceperez, Daniel ReynosovelascoAbstract:Two new species of Macrodactylus Dejean (Coleoptera: Scarabaeidae: Melolonthinae: Macrodactylini) from Mexico and Guatemala are described and illustrated: Macrodactylus miguelangeli new species from Mexico and Guatemala, and M. oaxacaensis new species from Mexico. With the descriptions of these two new species, the total number of species of the genus reaches 124. The characteristics of two closely related species are briefly discussed.
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review of the species of Macrodactylus dejean coleoptera scarabaeidae melolonthinae from the central american nucleus
Zootaxa, 2020Co-Authors: Roberto Arceperez, Miguel Ángel MorónAbstract:Twenty species of Macrodactylus Dejean (Coleoptera: Scarabaeidae: Melolonthinae: Macrodactylini) from the Central American nucleus are described and illustrated, including two new species from Guatemala: Macrodactylus eniocanoi new species and Macrodactylus escuintlaensis new species. Macrodactylus hondurensis Arce-Perez Moron is recorded for the first time from El Salvador. Diagnoses for 18 species previously recorded in the Central American nucleus are provided. A key to all known species of Macrodactylus of Central American nucleus and distributional maps are included.
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review of the species of Macrodactylus dejean coleoptera scarabaeidae melolonthinae from costa rica and panama
Zootaxa, 2018Co-Authors: Roberto Arceperez, Miguel Ángel MorónAbstract:Fifteen species of Macrodactylus Dejean (Coleoptera: Scarabaeidae: Melolonthinae: Macrodactylini) from Costa Rica and Panama are described and illustrated, including eight new species: M. latreillei new species., M. pseudothoracicus new species. and M. dejeani new species from Costa Rica; M. solisi new species from Costa Rica and Panama; M. martinezi new species from Costa Rica and Venezuela; M. chiriquiensis new species and M. kunayalaensis new species from Panama; and M. moroni new species from Costa Rica, and Panama. Diagnosis for seven species previously recorded in Costa Rica and Panama are included, and a key to all known species of Macrodactylus in these countries is provided.
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new species and records of Macrodactylus dejean coleoptera scarabaeidae melolonthinae macrodactylini from bolivia
Zootaxa, 2014Co-Authors: Roberto Arceperez, Miguel Ángel MorónAbstract:Two new species of Macrodactylus Dejean (Coleoptera: Scarabaeidae: Melolonthinae) from Bolivia are described and illustrated: M. megaphyllus new species (from Comarapa, Santa Cruz and Sehuenca, Cochabamba) and M. yunganus new species (from Mairana and Comarapa, Santa Cruz). In addition, the species Macrodactylus bolivianus Moser, M. gracilis Moser, and M. nobilis Frey are redescribed and illustrated to help facilitate identification of these species. A key to the 10 species of Macrodactylus presently known from Bolivia is provided.
M M Monastyrnaya - One of the best experts on this subject based on the ideXlab platform.
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study of the interaction of trypsin inhibitor from the sea anemone radianthus Macrodactylus with proteases
Biochemistry (moscow) Supplement Series B: Biomedical Chemistry, 2007Co-Authors: I N Sokotun, O V Gnedenko, A A Molnar, M M Monastyrnaya, E P Kozlovskaya, A V Leychenko, A S IvanovAbstract:The interaction of the inhibitor VJ (InhVJ), isolated from sea anemone R. Macrodactylus, with different proteases was investigated using the method of biosensor analysis. The following enzymes were tested: serine proteases (trypsin, α-chymotrypsin, plasmin, thrombin, kallikrein), cysteina protease (papain) and aspartic protease (pepsin). In the rage of the concentrations studied (10–400 nM) inhibitor VJ interacted only with trypsin and α-chymotrypsin. The intermolecular complexes formation between inhibitor VJ and each of these enzymes was characterized by the following kinetic and thermodynamics parameters: KD = 7.38 × 10−8 M and 9.93 × 10−7 M for pairs InhVJ/trypsin and InhVJ/α-chymotrypsin, respectively.
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proteinase inhibitors from the tropical sea anemone radianthus Macrodactylus isolation and characteristic
Biochemistry, 2007Co-Authors: I N Sokotun, Elena Leychenko, M M Monastyrnaya, A P Ilina, A A Eskov, S D Anastuk, E P KozlovskayaAbstract:Two new serine proteinase inhibitors (RmIn I and RmIn II) from the tropical sea anemone Radianthus Macrodactylus have been isolated and characterized. The purification procedure includes polychrome-1 hydrophobic chromatography, Superdex™ Peptide 10/30 FPLC, and Nucleosil C18 reverse-phase HPLC. The molecular masses of RmIn I, RmIn II, and the complexes RmIn II/trypsin and RmIn I,II/α-chymotrypsin have been determined. The K i values of RmIn I and RmIn II for trypsin and α-chymotrypsin have been determined. The polypeptides RmIn I and RmIn II are shown to be nontoxic and to exhibit antihistamine activity. The N-terminal amino acid sequences of RmIn I (GICSEPIVVGPCKAG-) and RmIn II (GSTCLEPKVVGPCKA-) have been determined. A high homology of the amino acid sequences is demonstrated for the proteinase inhibitors produced by such evolutionarily distant species as coelenterates, reptiles, and mammals.
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amino acid sequence of rtx a s isoform actinoporin from the sea anemone radianthus Macrodactylus
Toxicon, 2006Co-Authors: Anna Ilina, A V Lipkin, Ekaterina V Barsova, Marina P Issaeva, Elena Leychenko, Konstantin V Guzev, M M Monastyrnaya, Sergey Lukyanov, E P KozlovskayaAbstract:The amino acid sequence of actinoporin RTX-A (175 aa) from the sea anemone Radianthus Macrodactylus was determined by sequencing of clones obtained via amplification of cDNA. It was established that RTX-A possessed high homology with HmgIII from Heteractis magnifica (87%) and StI, StII from Stichodactyla helianthus (84 and 87%, respectively). The analysis of structural and functional relationships within RTX-A was carried out. The some disagreement concerning to significant role of several amino acid residues for actinoporins exhibition of hemolytic activity was found.
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biologically active polypeptides from the tropical sea anemone radianthus Macrodactylus
Toxicon, 2002Co-Authors: M M Monastyrnaya, Tatyana A Zykova, Olga V Apalikova, Tatyana V Shwets, E P KozlovskayaAbstract:Abstract Some biologically active polypeptides, three high and two low molecular weight cytolysins and four trypsin inhibitors were isolated from the sea anemone Radianthus Macrodactylus and characterized. The purification steps involved acetone precipitation, gel filtration, ion-exchange, and affinity chromatography, and ion-exchange and reverse-phase HPLC. The relative molecular weight of high molecular weight Radianthus cytolysins named according to their N-terminal amino acids RTX-A (Ala), RTX-S (Ser) and RTX-G (Gly) was about 20,000. The isoelectric points were 9.8 for RTX-A and RTX-S, and 10.5 for RTX-G. The hemolytic activities of RTX-A, RTX-S and RTX-G were 3.5×104, 5.0×104, and 1.0×104 HU/mg, respectively, and were inhibited by sphingomyelin. The N-terminal amino acid sequence of RTX-A was determined as ALAGAIIAGAGLGLKILIEVLGEG–VKVKI–. Molecular weight of low molecular weight Radianthus cytolysins RmI, RmII, and of one trypsin inhibitor InI were 5100, 6100 and 7100, respectively. Isoelectric points for RmI and RmII were 9.2 and 9.3. Their hemolytic activity worked out 25 and 20 HU/mg, and was not inhibited by sphingomyelin. Toxicity of RmI and RmII was assessed by their histaminolytic activity. Amino acid composition of RmI and RmII was similar to that of tealiatoxin, histaminolytic cytolysin from the sea anemone Tealia felina.
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calorimetric study of interactions of toxin from radianthus Macrodactylus with erythrocyte membrane
Biochemistry international, 1992Co-Authors: V L Shnyrov, M M Monastyrnaya, G G Zhadan, S M Kuznetsova, E P KozlovskayaAbstract:Differential scanning microcalorimetry, intrinsic protein fluorescence and SDS-electrophoresis have been applied for the study of the hemolytic toxin from sea anemone, Radianthus Macrodactylus, when it is incorporated into pure sphingomyelin/water systems and upon its effect on the human and dog erythrocyte membranes. The results obtained by using these techniques showed that one molecule of toxin withdraws six sphingomyelin molecules from the cooperative transition. Effect of hemolytic toxin on human erythrocyte ghosts causes an appearance of a new heat sorption peak in their differential scanning calorimetric curve with a maximum of 36 degrees C and, moreover, it leads to a loss of one of cytoskeleton proteins (actin). These effects are essentially weaker in the case of dog erythrocyte ghosts. This suggests differences in the structural organization of human and dog erythrocyte membranes.
Enrique Gonzalezortegon - One of the best experts on this subject based on the ideXlab platform.
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assessment of the interaction between the white shrimp palaemon longirostris and the exotic oriental shrimp palaemon Macrodactylus in a european estuary sw spain
Biological Invasions, 2010Co-Authors: Enrique Gonzalezortegon, Jose A Cuesta, E Pascual, Pilar DrakeAbstract:Results of field surveys, stomach content analysis, and laboratory measurements of oxygen consumption at different salinities in the exotic shrimp Palaemon Macrodactylus and its native counterpart P. longirostris from the Guadalquivir estuary (SW Spain) were used to assess interaction between both species. After its first record in this estuary in 1998, P. Macrodactylus has shown a gradual increase in density and its estuarine population has been clearly dominated by juveniles. Feeding habits of both shrimps indicated a strong trophic overlap between them, with mysids as main prey. Their salinity-related and spatial distribution patterns show that maximal inter-specific overlap between the two populations occurs in the inner, less saline part of the estuary. Specific oxygen consumption rates under different salinities and water oxygen concentrations suggest a more efficient metabolism and a higher tolerance to hypoxic conditions in brackish waters by P. Macrodactylus than by P. longirostris. Such inter-specific physiological differences could have made it possible for the exotic P. Macrodactylus to invade an estuarine stretch that, prior to its arrival, was infra-utilized by native species.
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first report of the oriental shrimp palaemon Macrodactylus rathbun 1902 decapoda caridea palaemonidae from german waters
Helgoland Marine Research, 2007Co-Authors: Enrique Gonzalezortegon, Jose A Cuesta, Christoph D SchubartAbstract:The native East Asian shrimp Palaemon Macrodactylus has become a common inhabitant of estuaries along the Pacific coast of North America. More recently (documented since 1999), the species has also been colonising European waters and has been reported from Spain, England, Belgium and the Netherlands. In this study, we present a chronology of the reported introductions of this species and provide the first detailed report of its occurrence in German waters. P. Macrodactylus was found in the Geeste river mouth (Weser Estuary) as well as in Hooksiel, north of Wilhelmshaven between 2004 and 2005. We assume its presence in other estuarine habitats of the North Sea and predict its introduction into the Baltic Sea.
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an illustrated key to species of palaemon and palaemonetes crustacea decapoda caridea from european waters including the alien species palaemon Macrodactylus
Journal of the Marine Biological Association of the United Kingdom, 2006Co-Authors: Enrique Gonzalezortegon, Jose A CuestaAbstract:A detailed illustrated key to the identi¢cation of the European species of the genera Palaemon and Palaemonetes is provided.This key has been elaborated taking into account recent changes in the species composition of both genera for European waters, such as the invalidity of Palaemon garciacidi, which is a synonym of P. longirostris, and the presence of an introduced species, Palaemon Macrodactylus that has been recently recorded at diierent localities along the Atlantic coast. The key is based on external morphological characters to facilitate its use by non-specialists. Data on the coloration, habitat and distribution of all species are also given.
