The Experts below are selected from a list of 4038 Experts worldwide ranked by ideXlab platform
Tsutomu Kawabe - One of the best experts on this subject based on the ideXlab platform.
-
Macrophage Derived Chemokine in malignant and tuberculous pleural effusions
Respirology, 2007Co-Authors: Masakazu Okamoto, Kazuyoshi Imaizumi, Yoshinori Hasegawa, Naozumi Hashimoto, Atsushi Sumida, Masataka Shibazaki, Kenzo Takagi, Kaoru Shimokata, Tsutomu KawabeAbstract:Background and objectives: Macrophage-Derived Chemokine (MDC/CCL22) is recognized as a T-helper (Th) 2-type Chemokine. Both malignant and tuberculous pleural effusions are typically lymphocytic pleural effusions. Tuberculous pleural effusions have a more polarized Th1 reaction than malignant effusions, which are predominantly Th2 in nature. The aim of this study was to compare the levels of MDC in malignant pleural effusions with those in tuberculous pleural effusions to help delineate the role of MDC in Th2 versus Th1 effusions. Methods: Forty-three patients with pleural effusions (32 malignant, 11 tuberculous) were studied. The concentration of MDC in the pleural effusion was measured by ELISA. Results: The median concentration of MDC was lower in malignant pleural effusions than in tuberculous pleural effusions (P < 0.005). Conclusions: MDC has been reported to both promote and suppress antitumour immunity. The low concentration of MDC in malignant effusions is likely to minimise its antitumour activity but the precise role of MDC in malignant and tuberculous effusions needs to be investigated further.
-
Macrophage-Derived Chemokine in malignant and tuberculous pleural effusions
Respirology (Carlton Vic.), 2007Co-Authors: Masakazu Okamoto, Kazuyoshi Imaizumi, Yoshinori Hasegawa, Naozumi Hashimoto, Atsushi Sumida, Masataka Shibazaki, Kenzo Takagi, Kaoru Shimokata, Tsutomu KawabeAbstract:Background and objectives: Macrophage-Derived Chemokine (MDC/CCL22) is recognized as a T-helper (Th) 2-type Chemokine. Both malignant and tuberculous pleural effusions are typically lymphocytic pleural effusions. Tuberculous pleural effusions have a more polarized Th1 reaction than malignant effusions, which are predominantly Th2 in nature. The aim of this study was to compare the levels of MDC in malignant pleural effusions with those in tuberculous pleural effusions to help delineate the role of MDC in Th2 versus Th1 effusions. Methods: Forty-three patients with pleural effusions (32 malignant, 11 tuberculous) were studied. The concentration of MDC in the pleural effusion was measured by ELISA. Results: The median concentration of MDC was lower in malignant pleural effusions than in tuberculous pleural effusions (P
-
Expression of Macrophage-Derived Chemokine (MDC)/CCL22 in human lung cancer
Cancer Immunology Immunotherapy, 2006Co-Authors: Toru Nakanishi, Masakazu Okamoto, Kazuyoshi Imaizumi, Yoshinori Hasegawa, Naozumi Hashimoto, Tsutomu Kawabe, Kaoru ShimokataAbstract:Background : Ligands for CXCR3 Chemokines [IFN-γ-inducible protein of 10 kD (IP-10/CXCL10), monokine induced by IFN-γ (Mig/CXCL9), IFN-inducible T cell α chemoattractant (I-TAC/CXCL11)] and those for CCR4 [Macrophage-Derived Chemokine (MDC/CCL22), thymus- and activation-regulated Chemokine (TARC/CCL17)] have been shown to play the central roles for T helper-cell recruitment into the tissues. To examine the role of these Chemokines in tumor progression of lung cancer, we investigated their expression in human lung cancer tissues to determine the possible relationship between their expression and the prognosis of patients. Methods : Total RNA was prepared from lung cancer tissues of 40 patients (24 adenocarcinoma and 16 squamous cell carcinoma). We measured gene expression levels of Chemokines (IP-10, Mig, I-TAC, MDC and TARC) by real-time quantitative RT-PCR. Results : Higher gene expression of MDC in lung cancer was significantly correlated with longer disease-free survival time and lower risk of recurrence after tumor resection. We could not find any significant relationship of IP-10, Mig, I-TAC and TARC gene expression with disease-free survival or lower risk of recurrence after surgery. Conclusions : These results suggest that increased gene expression of MDC in tumor tissues may be a predictive marker for improving the prognosis of lung cancer.
Patrick W. Gray - One of the best experts on this subject based on the ideXlab platform.
-
IFN‐γ‐inducible expression of thymus and activation‐regulated Chemokine/CCL17 and Macrophage‐Derived Chemokine/CCL22 in epidermal keratinocytes and their roles in atopic dermatitis
International immunology, 2002Co-Authors: Tatsuya Horikawa, David Chantry, Takashi Nakayama, Ichiro Hikita, Hidekazu Yamada, Ryuichi Fujisawa, Toshinori Bito, Susumu Harada, Atsushi Fukunaga, Patrick W. GrayAbstract:Thymus and activation-regulated Chemokine (TARC)/CCL17 and Macrophage-Derived Chemokine (MDC)/CCL22 are a pair of CC Chemokines known to selectively attract T(h)2 type memory T cells via CCR4. Here we examined circulating levels of TARC and MDC in patients with atopic dermatitis (AD) and control subjects by using plasma samples, which reflect blood contents of Chemokines more accurately than serum samples. The plasma levels of TARC and MDC were significantly elevated in AD patients. These values also strongly correlated with disease severity and serum lactate dehydrogenase levels, and weakly correlated with serum total IgE levels and blood eosinophilia. Previous studies demonstrated TARC immunoreactivity in the epidermal layer of AD lesional skin and production of TARC by a human keratinocytic cell line HaCaT upon stimulation with IFN-gamma. Here we demonstrated MDC immunoreactivity in the epidermal layer of AD skin at levels stronger than that of TARC. Furthermore, primary epidermal keratinocytes expressed both TARC and MDC mRNA upon stimulation with IFN-gamma, but efficiently secreted only MDC. These results suggest a post-transcriptional regulation in TARC production. IFN-gamma also induced TARC and MDC mRNA in mouse skin. Collectively, both TARC and MDC play important roles in the local accumulation of T(h)2 cells in AD lesional skin. Production of T(h)2-attracting Chemokines by epidermal keratinocytes upon treatment with IFN-gamma, which is also the potent inducer of T(h)1-attracting Chemokines, may underline the pivotal role of IFN-gamma in the chronic phase of AD where both T(h)1 and T(h)2 responses are mixed.
-
ifn γ inducible expression of thymus and activation regulated Chemokine ccl17 and Macrophage Derived Chemokine ccl22 in epidermal keratinocytes and their roles in atopic dermatitis
International Immunology, 2002Co-Authors: Tatsuya Horikawa, David Chantry, Takashi Nakayama, Ichiro Hikita, Hidekazu Yamada, Ryuichi Fujisawa, Toshinori Bito, Susumu Harada, Atsushi Fukunaga, Patrick W. GrayAbstract:Thymus and activation-regulated Chemokine (TARC)/CCL17 and Macrophage-Derived Chemokine (MDC)/CCL22 are a pair of CC Chemokines known to selectively attract T(h)2 type memory T cells via CCR4. Here we examined circulating levels of TARC and MDC in patients with atopic dermatitis (AD) and control subjects by using plasma samples, which reflect blood contents of Chemokines more accurately than serum samples. The plasma levels of TARC and MDC were significantly elevated in AD patients. These values also strongly correlated with disease severity and serum lactate dehydrogenase levels, and weakly correlated with serum total IgE levels and blood eosinophilia. Previous studies demonstrated TARC immunoreactivity in the epidermal layer of AD lesional skin and production of TARC by a human keratinocytic cell line HaCaT upon stimulation with IFN-gamma. Here we demonstrated MDC immunoreactivity in the epidermal layer of AD skin at levels stronger than that of TARC. Furthermore, primary epidermal keratinocytes expressed both TARC and MDC mRNA upon stimulation with IFN-gamma, but efficiently secreted only MDC. These results suggest a post-transcriptional regulation in TARC production. IFN-gamma also induced TARC and MDC mRNA in mouse skin. Collectively, both TARC and MDC play important roles in the local accumulation of T(h)2 cells in AD lesional skin. Production of T(h)2-attracting Chemokines by epidermal keratinocytes upon treatment with IFN-gamma, which is also the potent inducer of T(h)1-attracting Chemokines, may underline the pivotal role of IFN-gamma in the chronic phase of AD where both T(h)1 and T(h)2 responses are mixed.
-
presence of high contents of thymus and activation regulated Chemokine in platelets and elevated plasma levels of thymus and activation regulated Chemokine and Macrophage Derived Chemokine in patients with atopic dermatitis
The Journal of Allergy and Clinical Immunology, 2002Co-Authors: Takao Fujisawa, Takashi Nakayama, Ryuichi Fujisawa, Yoshiko Kato, Atsushi Morita, Hajime Katsumata, Hisashi Nishimori, K Iguchi, H Kamiya, Patrick W. GrayAbstract:Abstract Background: T H 2 cells and eosinophils selectively express CC Chemokine receptor 4 and CCR3, respectively, and their Chemokine ligands are likely to play important roles in the pathogenesis of atopic dermatitis (AD). Objective: The purpose of this study was to demonstrate the presence of thymus and activation-regulated Chemokine (TARC) in platelets and its release during clotting and to evaluate the circulating levels of TARC, Macrophage-Derived Chemokine (MDC), and eotaxin in control subjects and patients with AD. Methods: We compared plasma and serum contents of TARC, MDC, and eotaxin. We measured TARC contents in platelet lysates. We analyzed the correlation of plasma levels of TARC, MDC, and eotaxin with various clinicolaboratory parameters in patients with AD. Results: Serum contents of TARC rapidly increased during clotting, whereas those of MDC and eotaxin increased only slightly. We demonstrated that platelets contained TARC, and its levels were dramatically elevated in patients with AD. Platelets also released TARC on stimulation with thrombin. We therefore evaluated circulating levels of these Chemokines in control subjects and patients with AD by using plasma samples. Plasma TARC levels were significantly increased in patients with AD ( P r = 0.665, P r = 0.696, P = .00001), eosinophil counts ( r = 0.381, P = .007), and platelet counts ( r = 0.562, P P r = 0.727, P r = 0.861, P r = 0.505, P = .005), and platelet counts ( r = 0.370, P = .01). On treatment, plasma TARC and MDC levels were dramatically decreased in accordance with improved SCORAD scores ( P = .0012 and P = .0007, respectively). On the other hand, plasma eotaxin levels did not show any significant increase or correlation with any of the clinical parameters in patients with AD. Conclusion: Platelets from patients with AD contain high levels of TARC. Thus platelets might play an important role in AD pathogenesis by releasing T H 2-attracting TARC on activation. Furthermore, circulating levels of TARC and MDC, but not those of eotaxin, correlate well with the disease activity of AD. (J Allergy Clin Immunol 2002;110:139-46.)
-
Macrophage Derived Chemokine production by activated human t cells in vitro and in vivo preferential association with the production of type 2 cytokines
European Journal of Immunology, 2000Co-Authors: Grazia Galli, David Chantry, Patrick W. Gray, Paola Romagnani, Francesco Annunziato, Lorenzo Cosmi, Elena Lazzeri, Roberto Manetti, Enrico Maggi, Sergio RomagnaniAbstract:Macrophage-Derived Chemokine (MDC), a potent chemoattractant for chronically activated Th2 lymphocytes, is constitutively expressed by dendritic cells, B cells, Macrophages, and thymic medullary epithelial cells, whereas monocytes, NK cells, and T lymphocytes produce MDC only upon appropriate stimulation. In this study, we show in vitro MDC production also by activated T cells, which preferentially associate with the production of Th2 cytokines, IL-4, IL-5, and IL-6, and inversely correlate with the production of the Th1 cytokine, IFN-gamma. Moreover, high levels of MDC were detected in the sera of the great majority of subjects suffering from mycosis fungoides/Sezary syndrome or atopic dermatitis, which are considered as disorders characterized by the predominant expansion and activation of Th2 cells, respectively. By contrast, serum MDC levels in subjects with multiple sclerosis or Crohn's disease, which are characterized by a Th1 predominance, did not differ significantly from those of healthy controls. Finally, MDC expression was detected in the skin biopsy specimens of subjects with atopic dermatitis, where it was expressed by both dendritic cells and T lymphocytes. Taken together, these findings suggest that MDC production by activated T cells may occur both in vitro and in vivo, particularly in association with Th2 cytokines, thus providing an important amplification circuit for Th2-mediated responses.
-
Macrophage-Derived Chemokine is localized to thymic medullary epithelial cells and is a chemoattractant for CD3+, CD4+, CD8low thymocytes
Blood, 1999Co-Authors: David Chantry, Carol J. Raport, Christi L. Wood, Paola Romagnani, Angela Epp, Sergio Romagnani, Patrick W. GrayAbstract:Macrophage-Derived Chemokine (MDC) is a recently identified CC Chemokine that is a potent chemoattractant for dendritic cells, natural killer (NK) cells, and the Th2 subset of peripheral blood T cells. In normal tissues, MDC mRNA is expressed principally in the thymus. Immunohistochemical analysis performed on 5 human postnatal thymuses showed high MDC immunoreactivity, which was selectively localized to epithelial cells within the medulla. To examine the effects of MDC on immature T cells, we have identified cDNA clones for mouse and rat MDC. Expression of MDC in murine tissues is also highly restricted, with significant levels of mRNA found only in the thymus. Thymocytes express high-affinity binding sites for MDC (kd = 0.7 nmol/L), and, in vitro, MDC is a chemoattractant for these cells. MDC-responsive murine thymocytes express mRNA for CCR4, a recently identified receptor for MDC. Phenotypic analysis of MDC-responsive cells shows that they are enriched for a subset of double-positive cells that express high levels of CD3 and CD4 and that have reduced levels of CD8. This subset of MDC-responsive cells is consistent with the observed expression of MDC within the medulla, because more mature cells are found there. MDC may therefore play a role in the migration of T-cell subsets during development within the thymus.
Masakazu Okamoto - One of the best experts on this subject based on the ideXlab platform.
-
Macrophage Derived Chemokine in malignant and tuberculous pleural effusions
Respirology, 2007Co-Authors: Masakazu Okamoto, Kazuyoshi Imaizumi, Yoshinori Hasegawa, Naozumi Hashimoto, Atsushi Sumida, Masataka Shibazaki, Kenzo Takagi, Kaoru Shimokata, Tsutomu KawabeAbstract:Background and objectives: Macrophage-Derived Chemokine (MDC/CCL22) is recognized as a T-helper (Th) 2-type Chemokine. Both malignant and tuberculous pleural effusions are typically lymphocytic pleural effusions. Tuberculous pleural effusions have a more polarized Th1 reaction than malignant effusions, which are predominantly Th2 in nature. The aim of this study was to compare the levels of MDC in malignant pleural effusions with those in tuberculous pleural effusions to help delineate the role of MDC in Th2 versus Th1 effusions. Methods: Forty-three patients with pleural effusions (32 malignant, 11 tuberculous) were studied. The concentration of MDC in the pleural effusion was measured by ELISA. Results: The median concentration of MDC was lower in malignant pleural effusions than in tuberculous pleural effusions (P < 0.005). Conclusions: MDC has been reported to both promote and suppress antitumour immunity. The low concentration of MDC in malignant effusions is likely to minimise its antitumour activity but the precise role of MDC in malignant and tuberculous effusions needs to be investigated further.
-
Macrophage-Derived Chemokine in malignant and tuberculous pleural effusions
Respirology (Carlton Vic.), 2007Co-Authors: Masakazu Okamoto, Kazuyoshi Imaizumi, Yoshinori Hasegawa, Naozumi Hashimoto, Atsushi Sumida, Masataka Shibazaki, Kenzo Takagi, Kaoru Shimokata, Tsutomu KawabeAbstract:Background and objectives: Macrophage-Derived Chemokine (MDC/CCL22) is recognized as a T-helper (Th) 2-type Chemokine. Both malignant and tuberculous pleural effusions are typically lymphocytic pleural effusions. Tuberculous pleural effusions have a more polarized Th1 reaction than malignant effusions, which are predominantly Th2 in nature. The aim of this study was to compare the levels of MDC in malignant pleural effusions with those in tuberculous pleural effusions to help delineate the role of MDC in Th2 versus Th1 effusions. Methods: Forty-three patients with pleural effusions (32 malignant, 11 tuberculous) were studied. The concentration of MDC in the pleural effusion was measured by ELISA. Results: The median concentration of MDC was lower in malignant pleural effusions than in tuberculous pleural effusions (P
-
Expression of Macrophage-Derived Chemokine (MDC)/CCL22 in human lung cancer
Cancer Immunology Immunotherapy, 2006Co-Authors: Toru Nakanishi, Masakazu Okamoto, Kazuyoshi Imaizumi, Yoshinori Hasegawa, Naozumi Hashimoto, Tsutomu Kawabe, Kaoru ShimokataAbstract:Background : Ligands for CXCR3 Chemokines [IFN-γ-inducible protein of 10 kD (IP-10/CXCL10), monokine induced by IFN-γ (Mig/CXCL9), IFN-inducible T cell α chemoattractant (I-TAC/CXCL11)] and those for CCR4 [Macrophage-Derived Chemokine (MDC/CCL22), thymus- and activation-regulated Chemokine (TARC/CCL17)] have been shown to play the central roles for T helper-cell recruitment into the tissues. To examine the role of these Chemokines in tumor progression of lung cancer, we investigated their expression in human lung cancer tissues to determine the possible relationship between their expression and the prognosis of patients. Methods : Total RNA was prepared from lung cancer tissues of 40 patients (24 adenocarcinoma and 16 squamous cell carcinoma). We measured gene expression levels of Chemokines (IP-10, Mig, I-TAC, MDC and TARC) by real-time quantitative RT-PCR. Results : Higher gene expression of MDC in lung cancer was significantly correlated with longer disease-free survival time and lower risk of recurrence after tumor resection. We could not find any significant relationship of IP-10, Mig, I-TAC and TARC gene expression with disease-free survival or lower risk of recurrence after surgery. Conclusions : These results suggest that increased gene expression of MDC in tumor tissues may be a predictive marker for improving the prognosis of lung cancer.
Kaoru Shimokata - One of the best experts on this subject based on the ideXlab platform.
-
Macrophage Derived Chemokine in malignant and tuberculous pleural effusions
Respirology, 2007Co-Authors: Masakazu Okamoto, Kazuyoshi Imaizumi, Yoshinori Hasegawa, Naozumi Hashimoto, Atsushi Sumida, Masataka Shibazaki, Kenzo Takagi, Kaoru Shimokata, Tsutomu KawabeAbstract:Background and objectives: Macrophage-Derived Chemokine (MDC/CCL22) is recognized as a T-helper (Th) 2-type Chemokine. Both malignant and tuberculous pleural effusions are typically lymphocytic pleural effusions. Tuberculous pleural effusions have a more polarized Th1 reaction than malignant effusions, which are predominantly Th2 in nature. The aim of this study was to compare the levels of MDC in malignant pleural effusions with those in tuberculous pleural effusions to help delineate the role of MDC in Th2 versus Th1 effusions. Methods: Forty-three patients with pleural effusions (32 malignant, 11 tuberculous) were studied. The concentration of MDC in the pleural effusion was measured by ELISA. Results: The median concentration of MDC was lower in malignant pleural effusions than in tuberculous pleural effusions (P < 0.005). Conclusions: MDC has been reported to both promote and suppress antitumour immunity. The low concentration of MDC in malignant effusions is likely to minimise its antitumour activity but the precise role of MDC in malignant and tuberculous effusions needs to be investigated further.
-
Macrophage-Derived Chemokine in malignant and tuberculous pleural effusions
Respirology (Carlton Vic.), 2007Co-Authors: Masakazu Okamoto, Kazuyoshi Imaizumi, Yoshinori Hasegawa, Naozumi Hashimoto, Atsushi Sumida, Masataka Shibazaki, Kenzo Takagi, Kaoru Shimokata, Tsutomu KawabeAbstract:Background and objectives: Macrophage-Derived Chemokine (MDC/CCL22) is recognized as a T-helper (Th) 2-type Chemokine. Both malignant and tuberculous pleural effusions are typically lymphocytic pleural effusions. Tuberculous pleural effusions have a more polarized Th1 reaction than malignant effusions, which are predominantly Th2 in nature. The aim of this study was to compare the levels of MDC in malignant pleural effusions with those in tuberculous pleural effusions to help delineate the role of MDC in Th2 versus Th1 effusions. Methods: Forty-three patients with pleural effusions (32 malignant, 11 tuberculous) were studied. The concentration of MDC in the pleural effusion was measured by ELISA. Results: The median concentration of MDC was lower in malignant pleural effusions than in tuberculous pleural effusions (P
-
Expression of Macrophage-Derived Chemokine (MDC)/CCL22 in human lung cancer
Cancer Immunology Immunotherapy, 2006Co-Authors: Toru Nakanishi, Masakazu Okamoto, Kazuyoshi Imaizumi, Yoshinori Hasegawa, Naozumi Hashimoto, Tsutomu Kawabe, Kaoru ShimokataAbstract:Background : Ligands for CXCR3 Chemokines [IFN-γ-inducible protein of 10 kD (IP-10/CXCL10), monokine induced by IFN-γ (Mig/CXCL9), IFN-inducible T cell α chemoattractant (I-TAC/CXCL11)] and those for CCR4 [Macrophage-Derived Chemokine (MDC/CCL22), thymus- and activation-regulated Chemokine (TARC/CCL17)] have been shown to play the central roles for T helper-cell recruitment into the tissues. To examine the role of these Chemokines in tumor progression of lung cancer, we investigated their expression in human lung cancer tissues to determine the possible relationship between their expression and the prognosis of patients. Methods : Total RNA was prepared from lung cancer tissues of 40 patients (24 adenocarcinoma and 16 squamous cell carcinoma). We measured gene expression levels of Chemokines (IP-10, Mig, I-TAC, MDC and TARC) by real-time quantitative RT-PCR. Results : Higher gene expression of MDC in lung cancer was significantly correlated with longer disease-free survival time and lower risk of recurrence after tumor resection. We could not find any significant relationship of IP-10, Mig, I-TAC and TARC gene expression with disease-free survival or lower risk of recurrence after surgery. Conclusions : These results suggest that increased gene expression of MDC in tumor tissues may be a predictive marker for improving the prognosis of lung cancer.
Kazuyoshi Imaizumi - One of the best experts on this subject based on the ideXlab platform.
-
Macrophage Derived Chemokine in malignant and tuberculous pleural effusions
Respirology, 2007Co-Authors: Masakazu Okamoto, Kazuyoshi Imaizumi, Yoshinori Hasegawa, Naozumi Hashimoto, Atsushi Sumida, Masataka Shibazaki, Kenzo Takagi, Kaoru Shimokata, Tsutomu KawabeAbstract:Background and objectives: Macrophage-Derived Chemokine (MDC/CCL22) is recognized as a T-helper (Th) 2-type Chemokine. Both malignant and tuberculous pleural effusions are typically lymphocytic pleural effusions. Tuberculous pleural effusions have a more polarized Th1 reaction than malignant effusions, which are predominantly Th2 in nature. The aim of this study was to compare the levels of MDC in malignant pleural effusions with those in tuberculous pleural effusions to help delineate the role of MDC in Th2 versus Th1 effusions. Methods: Forty-three patients with pleural effusions (32 malignant, 11 tuberculous) were studied. The concentration of MDC in the pleural effusion was measured by ELISA. Results: The median concentration of MDC was lower in malignant pleural effusions than in tuberculous pleural effusions (P < 0.005). Conclusions: MDC has been reported to both promote and suppress antitumour immunity. The low concentration of MDC in malignant effusions is likely to minimise its antitumour activity but the precise role of MDC in malignant and tuberculous effusions needs to be investigated further.
-
Macrophage-Derived Chemokine in malignant and tuberculous pleural effusions
Respirology (Carlton Vic.), 2007Co-Authors: Masakazu Okamoto, Kazuyoshi Imaizumi, Yoshinori Hasegawa, Naozumi Hashimoto, Atsushi Sumida, Masataka Shibazaki, Kenzo Takagi, Kaoru Shimokata, Tsutomu KawabeAbstract:Background and objectives: Macrophage-Derived Chemokine (MDC/CCL22) is recognized as a T-helper (Th) 2-type Chemokine. Both malignant and tuberculous pleural effusions are typically lymphocytic pleural effusions. Tuberculous pleural effusions have a more polarized Th1 reaction than malignant effusions, which are predominantly Th2 in nature. The aim of this study was to compare the levels of MDC in malignant pleural effusions with those in tuberculous pleural effusions to help delineate the role of MDC in Th2 versus Th1 effusions. Methods: Forty-three patients with pleural effusions (32 malignant, 11 tuberculous) were studied. The concentration of MDC in the pleural effusion was measured by ELISA. Results: The median concentration of MDC was lower in malignant pleural effusions than in tuberculous pleural effusions (P
-
Expression of Macrophage-Derived Chemokine (MDC)/CCL22 in human lung cancer
Cancer Immunology Immunotherapy, 2006Co-Authors: Toru Nakanishi, Masakazu Okamoto, Kazuyoshi Imaizumi, Yoshinori Hasegawa, Naozumi Hashimoto, Tsutomu Kawabe, Kaoru ShimokataAbstract:Background : Ligands for CXCR3 Chemokines [IFN-γ-inducible protein of 10 kD (IP-10/CXCL10), monokine induced by IFN-γ (Mig/CXCL9), IFN-inducible T cell α chemoattractant (I-TAC/CXCL11)] and those for CCR4 [Macrophage-Derived Chemokine (MDC/CCL22), thymus- and activation-regulated Chemokine (TARC/CCL17)] have been shown to play the central roles for T helper-cell recruitment into the tissues. To examine the role of these Chemokines in tumor progression of lung cancer, we investigated their expression in human lung cancer tissues to determine the possible relationship between their expression and the prognosis of patients. Methods : Total RNA was prepared from lung cancer tissues of 40 patients (24 adenocarcinoma and 16 squamous cell carcinoma). We measured gene expression levels of Chemokines (IP-10, Mig, I-TAC, MDC and TARC) by real-time quantitative RT-PCR. Results : Higher gene expression of MDC in lung cancer was significantly correlated with longer disease-free survival time and lower risk of recurrence after tumor resection. We could not find any significant relationship of IP-10, Mig, I-TAC and TARC gene expression with disease-free survival or lower risk of recurrence after surgery. Conclusions : These results suggest that increased gene expression of MDC in tumor tissues may be a predictive marker for improving the prognosis of lung cancer.
