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Makoto Uchiyama - One of the best experts on this subject based on the ideXlab platform.
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the sialoadhesin cd169 expressing a Macrophage Subset in human proliferative glomerulonephritis
Nephrology Dialysis Transplantation, 2005Co-Authors: Yohei Ikezumi, Toshiaki Suzuki, Shinichi Hayafuji, Soichiro Okubo, David J Nikolicpaterson, Hiroshi Kawachi, Fujio Shimizu, Makoto UchiyamaAbstract:Background. Sialoadhesin (Sn; CD169) is a lectinlike receptor whose expression is restricted to Subsets of tissue and inflammatory Macrophages. We have previously identified accumulation of Snþ Macrophages as an important marker of disease progression versus remission in rat mesangial proliferative nephritis. The current study examined the significance of Snþ Macrophages in human proliferative glomerulonephritis. Methods. Frozen kidney sections from normal adult human kidney (n ¼ 4) and pediatric nephropathy (n ¼ 40) were stained for total Macrophages (CD68þ cells), Snþ Macrophages, CD3þ T-cells and collagen type I by immunofluorescence. Leukocyte infiltration and the severity of glomerular lesions and interstitial damage were scored. A second protocol biopsy was performed in 27 cases and clinical and biopsy-based data obtained. Results. Snþ Macrophages were absent from glomeruli in normal adult human kidney and in thin basement membrane disease (n ¼ 4), but were detected in 4 of 9 cases of purpura nephritis; 7 of 17 IgA nephropathy; 5 of 5 membranoproliferative glomerulonephritis, and 5 of 5 lupus nephritis. Snþ Macrophages were localized in areas of focal glomerular and interstitial damage. Two-colour immunostaining confirmed that Snþ cells are a Subset of total CD68þ Macrophages. The number of glomerular Snþ Macrophages correlated with the degree of proteinuria and glomerular lesions (r ¼ 0.44, P ¼ 0.0045 and r ¼ 0.82, P<0.0001; respectively), while interstitial Snþ Macrophages correlated with the degree of proteinuria and interstitial damage (r ¼ 0.59, P<0.0001 and r ¼ 0.75, P<0.0001; respectively). Combined immunostaining revealed that interstitial Snþ Macrophages and CD3þ T-cells co-localized in areas of tubulointerstitial damage with increased type I collagen deposition. There was significant correlation between the number of interstitial Snþ Macrophages and CD3þ T-cells (r ¼ 0.74, P<0.0001). Most patients responded to a 2 year period of glucocorticoid therapy with a reduction in proteinuria and glomerular lesions and this correlated with the reduction in the number of glomerular Snþ Macrophages. Conclusion. This study has identified Snþ cells as a Macrophage Subset whose accumulation in the kidney correlates with proteinuria and histologic damage. These results, together with recent findings from animal studies, suggest that Snþ Macrophages may play an important role in progressive renal disease.
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The sialoadhesin (CD169) expressing a Macrophage Subset in human proliferative glomerulonephritis
Nephrology Dialysis Transplantation, 2005Co-Authors: Yohei Ikezumi, Toshiaki Suzuki, Shinichi Hayafuji, Soichiro Okubo, Hiroshi Kawachi, Fujio Shimizu, David J. Nikolic-paterson, Makoto UchiyamaAbstract:Background. Sialoadhesin (Sn; CD169) is a lectinlike receptor whose expression is restricted to Subsets of tissue and inflammatory Macrophages. We have previously identified accumulation of Snþ Macrophages as an important marker of disease progression versus remission in rat mesangial proliferative nephritis. The current study examined the significance of Snþ Macrophages in human proliferative glomerulonephritis. Methods. Frozen kidney sections from normal adult human kidney (n ¼ 4) and pediatric nephropathy (n ¼ 40) were stained for total Macrophages (CD68þ cells), Snþ Macrophages, CD3þ T-cells and collagen type I by immunofluorescence. Leukocyte infiltration and the severity of glomerular lesions and interstitial damage were scored. A second protocol biopsy was performed in 27 cases and clinical and biopsy-based data obtained. Results. Snþ Macrophages were absent from glomeruli in normal adult human kidney and in thin basement membrane disease (n ¼ 4), but were detected in 4 of 9 cases of purpura nephritis; 7 of 17 IgA nephropathy; 5 of 5 membranoproliferative glomerulonephritis, and 5 of 5 lupus nephritis. Snþ Macrophages were localized in areas of focal glomerular and interstitial damage. Two-colour immunostaining confirmed that Snþ cells are a Subset of total CD68þ Macrophages. The number of glomerular Snþ Macrophages correlated with the degree of proteinuria and glomerular lesions (r ¼ 0.44, P ¼ 0.0045 and r ¼ 0.82, P
Yohei Ikezumi - One of the best experts on this subject based on the ideXlab platform.
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the sialoadhesin cd169 expressing a Macrophage Subset in human proliferative glomerulonephritis
Nephrology Dialysis Transplantation, 2005Co-Authors: Yohei Ikezumi, Toshiaki Suzuki, Shinichi Hayafuji, Soichiro Okubo, David J Nikolicpaterson, Hiroshi Kawachi, Fujio Shimizu, Makoto UchiyamaAbstract:Background. Sialoadhesin (Sn; CD169) is a lectinlike receptor whose expression is restricted to Subsets of tissue and inflammatory Macrophages. We have previously identified accumulation of Snþ Macrophages as an important marker of disease progression versus remission in rat mesangial proliferative nephritis. The current study examined the significance of Snþ Macrophages in human proliferative glomerulonephritis. Methods. Frozen kidney sections from normal adult human kidney (n ¼ 4) and pediatric nephropathy (n ¼ 40) were stained for total Macrophages (CD68þ cells), Snþ Macrophages, CD3þ T-cells and collagen type I by immunofluorescence. Leukocyte infiltration and the severity of glomerular lesions and interstitial damage were scored. A second protocol biopsy was performed in 27 cases and clinical and biopsy-based data obtained. Results. Snþ Macrophages were absent from glomeruli in normal adult human kidney and in thin basement membrane disease (n ¼ 4), but were detected in 4 of 9 cases of purpura nephritis; 7 of 17 IgA nephropathy; 5 of 5 membranoproliferative glomerulonephritis, and 5 of 5 lupus nephritis. Snþ Macrophages were localized in areas of focal glomerular and interstitial damage. Two-colour immunostaining confirmed that Snþ cells are a Subset of total CD68þ Macrophages. The number of glomerular Snþ Macrophages correlated with the degree of proteinuria and glomerular lesions (r ¼ 0.44, P ¼ 0.0045 and r ¼ 0.82, P<0.0001; respectively), while interstitial Snþ Macrophages correlated with the degree of proteinuria and interstitial damage (r ¼ 0.59, P<0.0001 and r ¼ 0.75, P<0.0001; respectively). Combined immunostaining revealed that interstitial Snþ Macrophages and CD3þ T-cells co-localized in areas of tubulointerstitial damage with increased type I collagen deposition. There was significant correlation between the number of interstitial Snþ Macrophages and CD3þ T-cells (r ¼ 0.74, P<0.0001). Most patients responded to a 2 year period of glucocorticoid therapy with a reduction in proteinuria and glomerular lesions and this correlated with the reduction in the number of glomerular Snþ Macrophages. Conclusion. This study has identified Snþ cells as a Macrophage Subset whose accumulation in the kidney correlates with proteinuria and histologic damage. These results, together with recent findings from animal studies, suggest that Snþ Macrophages may play an important role in progressive renal disease.
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The sialoadhesin (CD169) expressing a Macrophage Subset in human proliferative glomerulonephritis
Nephrology Dialysis Transplantation, 2005Co-Authors: Yohei Ikezumi, Toshiaki Suzuki, Shinichi Hayafuji, Soichiro Okubo, Hiroshi Kawachi, Fujio Shimizu, David J. Nikolic-paterson, Makoto UchiyamaAbstract:Background. Sialoadhesin (Sn; CD169) is a lectinlike receptor whose expression is restricted to Subsets of tissue and inflammatory Macrophages. We have previously identified accumulation of Snþ Macrophages as an important marker of disease progression versus remission in rat mesangial proliferative nephritis. The current study examined the significance of Snþ Macrophages in human proliferative glomerulonephritis. Methods. Frozen kidney sections from normal adult human kidney (n ¼ 4) and pediatric nephropathy (n ¼ 40) were stained for total Macrophages (CD68þ cells), Snþ Macrophages, CD3þ T-cells and collagen type I by immunofluorescence. Leukocyte infiltration and the severity of glomerular lesions and interstitial damage were scored. A second protocol biopsy was performed in 27 cases and clinical and biopsy-based data obtained. Results. Snþ Macrophages were absent from glomeruli in normal adult human kidney and in thin basement membrane disease (n ¼ 4), but were detected in 4 of 9 cases of purpura nephritis; 7 of 17 IgA nephropathy; 5 of 5 membranoproliferative glomerulonephritis, and 5 of 5 lupus nephritis. Snþ Macrophages were localized in areas of focal glomerular and interstitial damage. Two-colour immunostaining confirmed that Snþ cells are a Subset of total CD68þ Macrophages. The number of glomerular Snþ Macrophages correlated with the degree of proteinuria and glomerular lesions (r ¼ 0.44, P ¼ 0.0045 and r ¼ 0.82, P
Toshiaki Suzuki - One of the best experts on this subject based on the ideXlab platform.
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the sialoadhesin cd169 expressing a Macrophage Subset in human proliferative glomerulonephritis
Nephrology Dialysis Transplantation, 2005Co-Authors: Yohei Ikezumi, Toshiaki Suzuki, Shinichi Hayafuji, Soichiro Okubo, David J Nikolicpaterson, Hiroshi Kawachi, Fujio Shimizu, Makoto UchiyamaAbstract:Background. Sialoadhesin (Sn; CD169) is a lectinlike receptor whose expression is restricted to Subsets of tissue and inflammatory Macrophages. We have previously identified accumulation of Snþ Macrophages as an important marker of disease progression versus remission in rat mesangial proliferative nephritis. The current study examined the significance of Snþ Macrophages in human proliferative glomerulonephritis. Methods. Frozen kidney sections from normal adult human kidney (n ¼ 4) and pediatric nephropathy (n ¼ 40) were stained for total Macrophages (CD68þ cells), Snþ Macrophages, CD3þ T-cells and collagen type I by immunofluorescence. Leukocyte infiltration and the severity of glomerular lesions and interstitial damage were scored. A second protocol biopsy was performed in 27 cases and clinical and biopsy-based data obtained. Results. Snþ Macrophages were absent from glomeruli in normal adult human kidney and in thin basement membrane disease (n ¼ 4), but were detected in 4 of 9 cases of purpura nephritis; 7 of 17 IgA nephropathy; 5 of 5 membranoproliferative glomerulonephritis, and 5 of 5 lupus nephritis. Snþ Macrophages were localized in areas of focal glomerular and interstitial damage. Two-colour immunostaining confirmed that Snþ cells are a Subset of total CD68þ Macrophages. The number of glomerular Snþ Macrophages correlated with the degree of proteinuria and glomerular lesions (r ¼ 0.44, P ¼ 0.0045 and r ¼ 0.82, P<0.0001; respectively), while interstitial Snþ Macrophages correlated with the degree of proteinuria and interstitial damage (r ¼ 0.59, P<0.0001 and r ¼ 0.75, P<0.0001; respectively). Combined immunostaining revealed that interstitial Snþ Macrophages and CD3þ T-cells co-localized in areas of tubulointerstitial damage with increased type I collagen deposition. There was significant correlation between the number of interstitial Snþ Macrophages and CD3þ T-cells (r ¼ 0.74, P<0.0001). Most patients responded to a 2 year period of glucocorticoid therapy with a reduction in proteinuria and glomerular lesions and this correlated with the reduction in the number of glomerular Snþ Macrophages. Conclusion. This study has identified Snþ cells as a Macrophage Subset whose accumulation in the kidney correlates with proteinuria and histologic damage. These results, together with recent findings from animal studies, suggest that Snþ Macrophages may play an important role in progressive renal disease.
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The sialoadhesin (CD169) expressing a Macrophage Subset in human proliferative glomerulonephritis
Nephrology Dialysis Transplantation, 2005Co-Authors: Yohei Ikezumi, Toshiaki Suzuki, Shinichi Hayafuji, Soichiro Okubo, Hiroshi Kawachi, Fujio Shimizu, David J. Nikolic-paterson, Makoto UchiyamaAbstract:Background. Sialoadhesin (Sn; CD169) is a lectinlike receptor whose expression is restricted to Subsets of tissue and inflammatory Macrophages. We have previously identified accumulation of Snþ Macrophages as an important marker of disease progression versus remission in rat mesangial proliferative nephritis. The current study examined the significance of Snþ Macrophages in human proliferative glomerulonephritis. Methods. Frozen kidney sections from normal adult human kidney (n ¼ 4) and pediatric nephropathy (n ¼ 40) were stained for total Macrophages (CD68þ cells), Snþ Macrophages, CD3þ T-cells and collagen type I by immunofluorescence. Leukocyte infiltration and the severity of glomerular lesions and interstitial damage were scored. A second protocol biopsy was performed in 27 cases and clinical and biopsy-based data obtained. Results. Snþ Macrophages were absent from glomeruli in normal adult human kidney and in thin basement membrane disease (n ¼ 4), but were detected in 4 of 9 cases of purpura nephritis; 7 of 17 IgA nephropathy; 5 of 5 membranoproliferative glomerulonephritis, and 5 of 5 lupus nephritis. Snþ Macrophages were localized in areas of focal glomerular and interstitial damage. Two-colour immunostaining confirmed that Snþ cells are a Subset of total CD68þ Macrophages. The number of glomerular Snþ Macrophages correlated with the degree of proteinuria and glomerular lesions (r ¼ 0.44, P ¼ 0.0045 and r ¼ 0.82, P
Shinichi Hayafuji - One of the best experts on this subject based on the ideXlab platform.
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the sialoadhesin cd169 expressing a Macrophage Subset in human proliferative glomerulonephritis
Nephrology Dialysis Transplantation, 2005Co-Authors: Yohei Ikezumi, Toshiaki Suzuki, Shinichi Hayafuji, Soichiro Okubo, David J Nikolicpaterson, Hiroshi Kawachi, Fujio Shimizu, Makoto UchiyamaAbstract:Background. Sialoadhesin (Sn; CD169) is a lectinlike receptor whose expression is restricted to Subsets of tissue and inflammatory Macrophages. We have previously identified accumulation of Snþ Macrophages as an important marker of disease progression versus remission in rat mesangial proliferative nephritis. The current study examined the significance of Snþ Macrophages in human proliferative glomerulonephritis. Methods. Frozen kidney sections from normal adult human kidney (n ¼ 4) and pediatric nephropathy (n ¼ 40) were stained for total Macrophages (CD68þ cells), Snþ Macrophages, CD3þ T-cells and collagen type I by immunofluorescence. Leukocyte infiltration and the severity of glomerular lesions and interstitial damage were scored. A second protocol biopsy was performed in 27 cases and clinical and biopsy-based data obtained. Results. Snþ Macrophages were absent from glomeruli in normal adult human kidney and in thin basement membrane disease (n ¼ 4), but were detected in 4 of 9 cases of purpura nephritis; 7 of 17 IgA nephropathy; 5 of 5 membranoproliferative glomerulonephritis, and 5 of 5 lupus nephritis. Snþ Macrophages were localized in areas of focal glomerular and interstitial damage. Two-colour immunostaining confirmed that Snþ cells are a Subset of total CD68þ Macrophages. The number of glomerular Snþ Macrophages correlated with the degree of proteinuria and glomerular lesions (r ¼ 0.44, P ¼ 0.0045 and r ¼ 0.82, P<0.0001; respectively), while interstitial Snþ Macrophages correlated with the degree of proteinuria and interstitial damage (r ¼ 0.59, P<0.0001 and r ¼ 0.75, P<0.0001; respectively). Combined immunostaining revealed that interstitial Snþ Macrophages and CD3þ T-cells co-localized in areas of tubulointerstitial damage with increased type I collagen deposition. There was significant correlation between the number of interstitial Snþ Macrophages and CD3þ T-cells (r ¼ 0.74, P<0.0001). Most patients responded to a 2 year period of glucocorticoid therapy with a reduction in proteinuria and glomerular lesions and this correlated with the reduction in the number of glomerular Snþ Macrophages. Conclusion. This study has identified Snþ cells as a Macrophage Subset whose accumulation in the kidney correlates with proteinuria and histologic damage. These results, together with recent findings from animal studies, suggest that Snþ Macrophages may play an important role in progressive renal disease.
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The sialoadhesin (CD169) expressing a Macrophage Subset in human proliferative glomerulonephritis
Nephrology Dialysis Transplantation, 2005Co-Authors: Yohei Ikezumi, Toshiaki Suzuki, Shinichi Hayafuji, Soichiro Okubo, Hiroshi Kawachi, Fujio Shimizu, David J. Nikolic-paterson, Makoto UchiyamaAbstract:Background. Sialoadhesin (Sn; CD169) is a lectinlike receptor whose expression is restricted to Subsets of tissue and inflammatory Macrophages. We have previously identified accumulation of Snþ Macrophages as an important marker of disease progression versus remission in rat mesangial proliferative nephritis. The current study examined the significance of Snþ Macrophages in human proliferative glomerulonephritis. Methods. Frozen kidney sections from normal adult human kidney (n ¼ 4) and pediatric nephropathy (n ¼ 40) were stained for total Macrophages (CD68þ cells), Snþ Macrophages, CD3þ T-cells and collagen type I by immunofluorescence. Leukocyte infiltration and the severity of glomerular lesions and interstitial damage were scored. A second protocol biopsy was performed in 27 cases and clinical and biopsy-based data obtained. Results. Snþ Macrophages were absent from glomeruli in normal adult human kidney and in thin basement membrane disease (n ¼ 4), but were detected in 4 of 9 cases of purpura nephritis; 7 of 17 IgA nephropathy; 5 of 5 membranoproliferative glomerulonephritis, and 5 of 5 lupus nephritis. Snþ Macrophages were localized in areas of focal glomerular and interstitial damage. Two-colour immunostaining confirmed that Snþ cells are a Subset of total CD68þ Macrophages. The number of glomerular Snþ Macrophages correlated with the degree of proteinuria and glomerular lesions (r ¼ 0.44, P ¼ 0.0045 and r ¼ 0.82, P
Soichiro Okubo - One of the best experts on this subject based on the ideXlab platform.
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the sialoadhesin cd169 expressing a Macrophage Subset in human proliferative glomerulonephritis
Nephrology Dialysis Transplantation, 2005Co-Authors: Yohei Ikezumi, Toshiaki Suzuki, Shinichi Hayafuji, Soichiro Okubo, David J Nikolicpaterson, Hiroshi Kawachi, Fujio Shimizu, Makoto UchiyamaAbstract:Background. Sialoadhesin (Sn; CD169) is a lectinlike receptor whose expression is restricted to Subsets of tissue and inflammatory Macrophages. We have previously identified accumulation of Snþ Macrophages as an important marker of disease progression versus remission in rat mesangial proliferative nephritis. The current study examined the significance of Snþ Macrophages in human proliferative glomerulonephritis. Methods. Frozen kidney sections from normal adult human kidney (n ¼ 4) and pediatric nephropathy (n ¼ 40) were stained for total Macrophages (CD68þ cells), Snþ Macrophages, CD3þ T-cells and collagen type I by immunofluorescence. Leukocyte infiltration and the severity of glomerular lesions and interstitial damage were scored. A second protocol biopsy was performed in 27 cases and clinical and biopsy-based data obtained. Results. Snþ Macrophages were absent from glomeruli in normal adult human kidney and in thin basement membrane disease (n ¼ 4), but were detected in 4 of 9 cases of purpura nephritis; 7 of 17 IgA nephropathy; 5 of 5 membranoproliferative glomerulonephritis, and 5 of 5 lupus nephritis. Snþ Macrophages were localized in areas of focal glomerular and interstitial damage. Two-colour immunostaining confirmed that Snþ cells are a Subset of total CD68þ Macrophages. The number of glomerular Snþ Macrophages correlated with the degree of proteinuria and glomerular lesions (r ¼ 0.44, P ¼ 0.0045 and r ¼ 0.82, P<0.0001; respectively), while interstitial Snþ Macrophages correlated with the degree of proteinuria and interstitial damage (r ¼ 0.59, P<0.0001 and r ¼ 0.75, P<0.0001; respectively). Combined immunostaining revealed that interstitial Snþ Macrophages and CD3þ T-cells co-localized in areas of tubulointerstitial damage with increased type I collagen deposition. There was significant correlation between the number of interstitial Snþ Macrophages and CD3þ T-cells (r ¼ 0.74, P<0.0001). Most patients responded to a 2 year period of glucocorticoid therapy with a reduction in proteinuria and glomerular lesions and this correlated with the reduction in the number of glomerular Snþ Macrophages. Conclusion. This study has identified Snþ cells as a Macrophage Subset whose accumulation in the kidney correlates with proteinuria and histologic damage. These results, together with recent findings from animal studies, suggest that Snþ Macrophages may play an important role in progressive renal disease.
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The sialoadhesin (CD169) expressing a Macrophage Subset in human proliferative glomerulonephritis
Nephrology Dialysis Transplantation, 2005Co-Authors: Yohei Ikezumi, Toshiaki Suzuki, Shinichi Hayafuji, Soichiro Okubo, Hiroshi Kawachi, Fujio Shimizu, David J. Nikolic-paterson, Makoto UchiyamaAbstract:Background. Sialoadhesin (Sn; CD169) is a lectinlike receptor whose expression is restricted to Subsets of tissue and inflammatory Macrophages. We have previously identified accumulation of Snþ Macrophages as an important marker of disease progression versus remission in rat mesangial proliferative nephritis. The current study examined the significance of Snþ Macrophages in human proliferative glomerulonephritis. Methods. Frozen kidney sections from normal adult human kidney (n ¼ 4) and pediatric nephropathy (n ¼ 40) were stained for total Macrophages (CD68þ cells), Snþ Macrophages, CD3þ T-cells and collagen type I by immunofluorescence. Leukocyte infiltration and the severity of glomerular lesions and interstitial damage were scored. A second protocol biopsy was performed in 27 cases and clinical and biopsy-based data obtained. Results. Snþ Macrophages were absent from glomeruli in normal adult human kidney and in thin basement membrane disease (n ¼ 4), but were detected in 4 of 9 cases of purpura nephritis; 7 of 17 IgA nephropathy; 5 of 5 membranoproliferative glomerulonephritis, and 5 of 5 lupus nephritis. Snþ Macrophages were localized in areas of focal glomerular and interstitial damage. Two-colour immunostaining confirmed that Snþ cells are a Subset of total CD68þ Macrophages. The number of glomerular Snþ Macrophages correlated with the degree of proteinuria and glomerular lesions (r ¼ 0.44, P ¼ 0.0045 and r ¼ 0.82, P
