The Experts below are selected from a list of 1206 Experts worldwide ranked by ideXlab platform
Carlos Ricardo Soccol - One of the best experts on this subject based on the ideXlab platform.
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A simplified model for A. Niger FS3 growth during phytase formation in solid State fermentation
Brazilian Archives of Biology and Technology, 2009Co-Authors: Michele Rigon Spier, Luiz Alberto Junior Letti, Adenise Lorenci Woiciechowski, Carlos Ricardo SoccolAbstract:A simplified model to describe fungal growth during citric pulp fermentation for phytase production was described for the first time. Experimental data for biomass growth were adjusted to classical mathematical growth models (Monod and Logistic). The Monod model predictions showed good agreement with the experimental results for biomass concentration during 96 hours of fermentation. Parameters such as yield of biomass from oxygen (YX/O), Maintenance Coefficient (m) and specific growth rate (µ) were compared showing a good correlation between the data and the model. An alternative method for biomass determination in this process was developed since a great correlation was found between biomass growth and enzyme formation.
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Relationship between coffee husk caffeine degradation and respiration of Aspergillus sp. LPBx in solid-state fermentation.
Applied Biochemistry and Biotechnology, 2002Co-Authors: Débora Brand, Sevastianos Roussos, Ashok Pandey, Jose A Rodriguez-leon, Ivo Brand, Carlos Ricardo SoccolAbstract:Studies were carried out in a packed-bed column fermentor using coffee husk as substrate in order to verify a relationship between caffeine degradation and the respiration of Aspergillus sp. LPBx. Fermentation conditions were optimized by using factorial design experiments. The kinetic study showed that the caffeine degradation was related to the development of mold and its respiration and also with the consumption of reducing sugars present in coffee husk. From the values obtained experimentally for oxygen uptake rate and CO2 evolved, we determined a biomass yield of 3.811 g of biomass/g of consumed O2 and a Maintenance Coefficient of 0.0031 g of consumed O2/(g of biomass x h). The maximum caffeine degradation achieved was 90%.
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Growth kinetics of Rhizopus arrhizus in solid state fermentation of treated cassava
Biotechnology Techniques, 1993Co-Authors: Carlos Ricardo Soccol, José A. Rodríguez León, Bernard Marin, Sevastianos Roussos, Maurice RaimbaultAbstract:Growth kinetics of Rhizopus arrhizus MUCL 28168 were determined for different treatments of cassava during solid state fermentation. The best case gave a specific growth rate (μ) of 0.24 h-1, a yield calculated on a basis that oxygen consumption (Yx/o) was 2.9 g biomass. g-1 O2 consumed and the Maintenance Coefficient (m) was 0.004 g O2 consumed. g-1 biomass. h-1.
Ferda Mavituna - One of the best experts on this subject based on the ideXlab platform.
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Actinorhodin production by Streptomyces coelicolor A3(2): kinetic parameters related to growth, substrate uptake and production
Applied Microbiology and Biotechnology, 1993Co-Authors: Kutlu Ozergin-ulgen, Ferda MavitunaAbstract:The dynamics of an Streptomyces coelicolor A3(2) culture in a 20-l computer-controlled batch bioreactor was investigated both experimentally and theoretically. In defined medium, depending on the initial conditions, the calculated value of some of the kinetic parameters were: maximum specific growth rate, 0.03 h^−1; death rate constant, 1.4–6.3 × 10^−3 h^−1; observed biomass yield, 0.21 g cells g^−1 glucose and the Maintenance Coefficient for the cells, 0.0448 g glucose g^−1 cells h^−1. According to both experimental observations and the Luedeking-Piret model, actinorhodin production was found to be growth-associated. This paper provides the first published quantitative information on the main kinetic parameters describing the activity of S. coelicolor in batch culture.
Saroj Mishra - One of the best experts on this subject based on the ideXlab platform.
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Development of a dual specific growth rate-based fed-batch process for production of recombinant human granulocyte colony-stimulating factor in Pichia pastoris
Bioprocess and Biosystems Engineering, 2020Co-Authors: Ashwani Gautam, Vikram Sahai, Saroj MishraAbstract:A number of limitations exist for production of human granulocyte colony-stimulating factor (G-CSF) in Pichia pastoris . In this study, two different specific growth rates (0.015 h^−1, 0.01 h^−1) were used sequentially in the mixed substrate feeding period during methanol induction phase to enhance the G-CSF titer in the culture broth. Necessary parameters required for implementing the feeding strategy, such as specific product yield on biomass ( Y _ P / X ) and Maintenance Coefficient ( m ) on glycerol, methanol, and sorbitol were estimated using continuous culture technique. Using this strategy, for the same volumetric productivity, about 20% increase in protein titer was achieved over that obtained from the run carried out at a single pre-set value of 0.015 h^−1 alone. Thus, implementation of higher specific growth rate (0.015 h^−1) set during initial stages of the methanol induction phase followed by a lower specific growth rate (0.01 h^−1) helped in achieving increased protein titers.
Kutlu Ozergin-ulgen - One of the best experts on this subject based on the ideXlab platform.
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Actinorhodin production by Streptomyces coelicolor A3(2): kinetic parameters related to growth, substrate uptake and production
Applied Microbiology and Biotechnology, 1993Co-Authors: Kutlu Ozergin-ulgen, Ferda MavitunaAbstract:The dynamics of an Streptomyces coelicolor A3(2) culture in a 20-l computer-controlled batch bioreactor was investigated both experimentally and theoretically. In defined medium, depending on the initial conditions, the calculated value of some of the kinetic parameters were: maximum specific growth rate, 0.03 h^−1; death rate constant, 1.4–6.3 × 10^−3 h^−1; observed biomass yield, 0.21 g cells g^−1 glucose and the Maintenance Coefficient for the cells, 0.0448 g glucose g^−1 cells h^−1. According to both experimental observations and the Luedeking-Piret model, actinorhodin production was found to be growth-associated. This paper provides the first published quantitative information on the main kinetic parameters describing the activity of S. coelicolor in batch culture.
Ashwani Gautam - One of the best experts on this subject based on the ideXlab platform.
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Development of a dual specific growth rate-based fed-batch process for production of recombinant human granulocyte colony-stimulating factor in Pichia pastoris
Bioprocess and Biosystems Engineering, 2020Co-Authors: Ashwani Gautam, Vikram Sahai, Saroj MishraAbstract:A number of limitations exist for production of human granulocyte colony-stimulating factor (G-CSF) in Pichia pastoris . In this study, two different specific growth rates (0.015 h^−1, 0.01 h^−1) were used sequentially in the mixed substrate feeding period during methanol induction phase to enhance the G-CSF titer in the culture broth. Necessary parameters required for implementing the feeding strategy, such as specific product yield on biomass ( Y _ P / X ) and Maintenance Coefficient ( m ) on glycerol, methanol, and sorbitol were estimated using continuous culture technique. Using this strategy, for the same volumetric productivity, about 20% increase in protein titer was achieved over that obtained from the run carried out at a single pre-set value of 0.015 h^−1 alone. Thus, implementation of higher specific growth rate (0.015 h^−1) set during initial stages of the methanol induction phase followed by a lower specific growth rate (0.01 h^−1) helped in achieving increased protein titers.
