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Sandro La Vignera - One of the best experts on this subject based on the ideXlab platform.
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Male Accessory Gland inflammation, infertility, and sexual dysfunctions: a practical approach to diagnosis and therapy.
Andrology, 2017Co-Authors: Aldo E. Calogero, Rosita A. Condorelli, Ylenia Duca, Sandro La VigneraAbstract:Summary The role of urogenital inflammation in causing infertility and sexual dysfunctions has long been a matter of debate in the international scientific literature. The most recent scientific evidences show that Male Accessory Gland infection/inflammation could alter, with various mechanisms, both conventional and biofunctional sperm parameters, and determine worst reproductive outcome. At the same time, the high prevalence of erectile dysfunction and premature ejaculation in patients with Male Accessory Gland infection/inflammation underlines the close link between these diseases and sexual dysfunctions. The aim of this review was to provide the reader the basis for a correct diagnosis of Male Accessory Gland infection/inflammation and a subsequent appropriate therapeutic approach, particularly in patients with infertility and/or sexual dysfunction.
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hormonal treatment with transdermal testosterone in patients with Male Accessory Gland inflammation magi effects on sperm parameters
Andrologia, 2017Co-Authors: Sandro La Vignera, Rosita A. Condorelli, Laura M. Mongioì, S G Vitale, Giovanna Russo, Antonio Simone Laganà, Aldo E. CalogeroAbstract:Summary Recently, it has been reported that treatment with testosterone (T) could have favourable effects on prostate inflammation; however, the data appear inconsistent. The main evidences concern experimental studies, and there is lower information obtainable from clinical studies. This study was conducted on patients with diagnosis of Male Accessory Gland infection (MAGI) and a concomitant hormonal condition of acquired hypergonadotropic hypogonadism and has evaluated the effects on sperm parameters of the administration of a transdermal formulation of T gel for 3 months. The treated patients showed a significantly increased percentage of spermatozoa with normal form and progressive motility (p < .05 vs baseline), a significant reduction of CD45pos leucocytes in the semen (p < .05 vs baseline) and finally a significant increase of the seminal concentrations of zinc, fructose and alpha-glucosidase (p < .05 vs baseline) identified as key parameters associated to secretory function of the Male Accessory Glands. The results of this study suggest the use of transdermal T in hypogonadal patients with MAGI for favourable effects on sperm parameters.
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Antioxidants in Male Accessory Gland Infection
Antioxidants in Andrology, 2016Co-Authors: Aldo E. Calogero, Rosita A. Condorelli, Sandro La VigneraAbstract:Male Accessory Gland infections (MAGI) have a negative impact on the secretory function of the Male Accessory Glands, hence altering seminal fluid. MAGI can be classified according to the presence/absence of microorganisms in microbial forms, when it is possible to identify the presence of microorganisms, and in inflammatory forms, characterized by leukocytospermia and/or overproduction of reactive oxygen species (ROS). The treatment of choice of the microbial forms of MAGI is based on antibiotic administration, followed by anti-inflammatory drugs and antioxidants. The inflammatory forms, characterized by leukocytospermia and ROS overproduction, are mainly treated with anti-inflammatory drugs followed by antioxidants. Antioxidants may be prescribed both to ameliorate the symptoms of MAGI and to reduce sperm oxidative stress-induced damage that can lead to Male infertility.
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Hormonal treatment with transdermal testosterone in patients with Male Accessory Gland inflammation (MAGI): Effects on sperm parameters.
Andrologia, 2016Co-Authors: Sandro La Vignera, Rosita A. Condorelli, Laura M. Mongioì, S G Vitale, Giovanna Russo, Antonio Simone Laganà, Aldo E. CalogeroAbstract:Summary Recently, it has been reported that treatment with testosterone (T) could have favourable effects on prostate inflammation; however, the data appear inconsistent. The main evidences concern experimental studies, and there is lower information obtainable from clinical studies. This study was conducted on patients with diagnosis of Male Accessory Gland infection (MAGI) and a concomitant hormonal condition of acquired hypergonadotropic hypogonadism and has evaluated the effects on sperm parameters of the administration of a transdermal formulation of T gel for 3 months. The treated patients showed a significantly increased percentage of spermatozoa with normal form and progressive motility (p
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acquired premature ejaculation and Male Accessory Gland infection relevance of ultrasound examination
Asian Journal of Andrology, 2016Co-Authors: Sandro La Vignera, Rosita A. Condorelli, Enzo Vicari, Vincenzo Favilla, Giuseppe Morgia, Aldo E. CalogeroAbstract:We have previously demonstrated a high frequency of premature ejaculation (PE) among patients with Male Accessory Gland infection (MAGI). The aim of this study was to evaluate the ultrasound (US) features of patients with MAGI and acquired premature ejaculation (APE) associated (MAGI-APEpos). US evaluation of 50 MAGI-APEpos patients compared to 50 patients with MAGI without PE (MAGI-PEneg) which represent the control group. The diagnosis of APE was made through the evaluation of Intravaginal ejaculation latency time (IELT) and confirmed with the questionnaire PEDT (Premature Ejaculation Diagnostic Tool). The main outcome measure was represented by the frequency of US criteria suggestive of P (prostatitis), V (vesiculitis), and E (epididymitis) in MAGI-APEpos and MAGI-PEneg patients. MAGI-APEpos patients showed a total number of US criteria significantly higher compared to MAGI-PEneg patients. MAGI-APEpos showed a higher frequency of US criteria of V and E (complicated forms of MAGI). Finally, in MAGI-APEpos group, it was found a positive relationship between the anteroposterior diameter (APD) of the caudal tract of the epididymis and the APD of the seminal vesicles, as well as between both diameters and the PEDT score. MAGI-APEpos patients have a peculiar US characterization compared to MAGI-PEneg patients. According to these results, US evaluation of the epididymal and of the prostato vesicular tract should be considered in the practical clinical approach of patients with MAGI and APE. In particular, it could be a support for a possible pathophysiological interpretation of this clinical problem in these patients.
Aldo E. Calogero - One of the best experts on this subject based on the ideXlab platform.
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Male Accessory Gland inflammation, infertility, and sexual dysfunctions: a practical approach to diagnosis and therapy.
Andrology, 2017Co-Authors: Aldo E. Calogero, Rosita A. Condorelli, Ylenia Duca, Sandro La VigneraAbstract:Summary The role of urogenital inflammation in causing infertility and sexual dysfunctions has long been a matter of debate in the international scientific literature. The most recent scientific evidences show that Male Accessory Gland infection/inflammation could alter, with various mechanisms, both conventional and biofunctional sperm parameters, and determine worst reproductive outcome. At the same time, the high prevalence of erectile dysfunction and premature ejaculation in patients with Male Accessory Gland infection/inflammation underlines the close link between these diseases and sexual dysfunctions. The aim of this review was to provide the reader the basis for a correct diagnosis of Male Accessory Gland infection/inflammation and a subsequent appropriate therapeutic approach, particularly in patients with infertility and/or sexual dysfunction.
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hormonal treatment with transdermal testosterone in patients with Male Accessory Gland inflammation magi effects on sperm parameters
Andrologia, 2017Co-Authors: Sandro La Vignera, Rosita A. Condorelli, Laura M. Mongioì, S G Vitale, Giovanna Russo, Antonio Simone Laganà, Aldo E. CalogeroAbstract:Summary Recently, it has been reported that treatment with testosterone (T) could have favourable effects on prostate inflammation; however, the data appear inconsistent. The main evidences concern experimental studies, and there is lower information obtainable from clinical studies. This study was conducted on patients with diagnosis of Male Accessory Gland infection (MAGI) and a concomitant hormonal condition of acquired hypergonadotropic hypogonadism and has evaluated the effects on sperm parameters of the administration of a transdermal formulation of T gel for 3 months. The treated patients showed a significantly increased percentage of spermatozoa with normal form and progressive motility (p < .05 vs baseline), a significant reduction of CD45pos leucocytes in the semen (p < .05 vs baseline) and finally a significant increase of the seminal concentrations of zinc, fructose and alpha-glucosidase (p < .05 vs baseline) identified as key parameters associated to secretory function of the Male Accessory Glands. The results of this study suggest the use of transdermal T in hypogonadal patients with MAGI for favourable effects on sperm parameters.
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Antioxidants in Male Accessory Gland Infection
Antioxidants in Andrology, 2016Co-Authors: Aldo E. Calogero, Rosita A. Condorelli, Sandro La VigneraAbstract:Male Accessory Gland infections (MAGI) have a negative impact on the secretory function of the Male Accessory Glands, hence altering seminal fluid. MAGI can be classified according to the presence/absence of microorganisms in microbial forms, when it is possible to identify the presence of microorganisms, and in inflammatory forms, characterized by leukocytospermia and/or overproduction of reactive oxygen species (ROS). The treatment of choice of the microbial forms of MAGI is based on antibiotic administration, followed by anti-inflammatory drugs and antioxidants. The inflammatory forms, characterized by leukocytospermia and ROS overproduction, are mainly treated with anti-inflammatory drugs followed by antioxidants. Antioxidants may be prescribed both to ameliorate the symptoms of MAGI and to reduce sperm oxidative stress-induced damage that can lead to Male infertility.
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Hormonal treatment with transdermal testosterone in patients with Male Accessory Gland inflammation (MAGI): Effects on sperm parameters.
Andrologia, 2016Co-Authors: Sandro La Vignera, Rosita A. Condorelli, Laura M. Mongioì, S G Vitale, Giovanna Russo, Antonio Simone Laganà, Aldo E. CalogeroAbstract:Summary Recently, it has been reported that treatment with testosterone (T) could have favourable effects on prostate inflammation; however, the data appear inconsistent. The main evidences concern experimental studies, and there is lower information obtainable from clinical studies. This study was conducted on patients with diagnosis of Male Accessory Gland infection (MAGI) and a concomitant hormonal condition of acquired hypergonadotropic hypogonadism and has evaluated the effects on sperm parameters of the administration of a transdermal formulation of T gel for 3 months. The treated patients showed a significantly increased percentage of spermatozoa with normal form and progressive motility (p
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acquired premature ejaculation and Male Accessory Gland infection relevance of ultrasound examination
Asian Journal of Andrology, 2016Co-Authors: La Vignera S, E Vicari, Rosita A. Condorelli, Giuseppe Morgia, Aldo E. CalogeroAbstract:We have previously demonstrated a high frequency of premature ejaculation (PE) among patients with Male Accessory Gland infection (MAGI). The aim of this study was to evaluate the ultrasound (US) features of patients with MAGI and acquired premature ejaculation (APE) associated (MAGI-APEpos). US evaluation of 50 MAGI-APEpos patients compared to 50 patients with MAGI without PE (MAGI-PEneg) which represent the control group. The diagnosis of APE was made through the evaluation of Intravaginal ejaculation latency time (IELT) and confirmed with the questionnaire PEDT (Premature Ejaculation Diagnostic Tool). The main outcome measure was represented by the frequency of US criteria suggestive of P (prostatitis), V (vesiculitis), and E (epididymitis) in MAGI-APEpos and MAGI-PEneg patients. MAGI-APEpos patients showed a total number of US criteria significantly higher compared to MAGI-PEneg patients. MAGI-APEpos showed a higher frequency of US criteria of V and E (complicated forms of MAGI). Finally, in MAGI-APEpos group, it was found a positive relationship between the anteroposterior diameter (APD) of the caudal tract of the epididymis and the APD of the seminal vesicles, as well as between both diameters and the PEDT score. MAGI-APEpos patients have a peculiar US characterization compared to MAGI-PEneg patients. According to these results, US evaluation of the epididymal and of the prostato vesicular tract should be considered in the practical clinical approach of patients with MAGI and APE. In particular, it could be a support for a possible pathophysiological interpretation of this clinical problem in these patients.
Rosita A. Condorelli - One of the best experts on this subject based on the ideXlab platform.
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high rate of detection of ultrasound signs of prostatitis in patients with hpv dna persistence on semen role of ultrasound in hpv related Male Accessory Gland infection
Journal of Endocrinological Investigation, 2019Co-Authors: S La Vignera, Rosita A. Condorelli, Vincenzo Favilla, Sebastiano Cimino, Laura M. Mongioì, Giovanna Russo, Guido Scalia, Rossella Cannarella, Filippo Giacone, Giuseppe MorgiaAbstract:Papillomavirus (HPV) often occurs in the semen of patients with Male Accessory Gland infection (MAGI). Ultrasound (US) evaluation has been suggested as a promising diagnostic tool for patients with HPV-related MAGI. No data on the spontaneous clearance of HPV-DNA have been reported so far in HPV-related MAGI. The primary aim of the study was to assess the percentage of early HPV-DNA spontaneous clearance in patients with prostatitis. The secondary aim was to evaluate the frequency of spontaneous clearance of HPV-DNA among patients with prostatitis associated with the presence or absence of US abnormalities. Patients with inflammatory MAGI and at least one suspicious criterion for HPV infection underwent semen HPV-DNA detection and prostate US. The presence of HPV-DNA was further investigated after a 6-month-long follow-up. Eighty patients satisfied the inclusion criteria and were recruited in the study. 69% of patients (55/80) showed HPV-DNA persistence in the semen. Among them, 82% (45/55) was positive for US signs of prostatitis, while they occurred only in 12% (3/25) of those patients with no sign of HPV-DNA persistence (p < 0.001). All patients with persistent high-risk HPV genotype (n = 30) showed at least two US signs of prostatitis. In 73% of patients (22/30), E6 and E7 mRNAs were detected. US signs of prostatitis more frequently occurred in patients with evidence of HPV-DNA persistence on semen, especially in those with high-risk genotypes. This highlights the importance of US in the framework of HPV-related MAGI.
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Male Accessory Gland inflammation, infertility, and sexual dysfunctions: a practical approach to diagnosis and therapy.
Andrology, 2017Co-Authors: Aldo E. Calogero, Rosita A. Condorelli, Ylenia Duca, Sandro La VigneraAbstract:Summary The role of urogenital inflammation in causing infertility and sexual dysfunctions has long been a matter of debate in the international scientific literature. The most recent scientific evidences show that Male Accessory Gland infection/inflammation could alter, with various mechanisms, both conventional and biofunctional sperm parameters, and determine worst reproductive outcome. At the same time, the high prevalence of erectile dysfunction and premature ejaculation in patients with Male Accessory Gland infection/inflammation underlines the close link between these diseases and sexual dysfunctions. The aim of this review was to provide the reader the basis for a correct diagnosis of Male Accessory Gland infection/inflammation and a subsequent appropriate therapeutic approach, particularly in patients with infertility and/or sexual dysfunction.
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hormonal treatment with transdermal testosterone in patients with Male Accessory Gland inflammation magi effects on sperm parameters
Andrologia, 2017Co-Authors: Sandro La Vignera, Rosita A. Condorelli, Laura M. Mongioì, S G Vitale, Giovanna Russo, Antonio Simone Laganà, Aldo E. CalogeroAbstract:Summary Recently, it has been reported that treatment with testosterone (T) could have favourable effects on prostate inflammation; however, the data appear inconsistent. The main evidences concern experimental studies, and there is lower information obtainable from clinical studies. This study was conducted on patients with diagnosis of Male Accessory Gland infection (MAGI) and a concomitant hormonal condition of acquired hypergonadotropic hypogonadism and has evaluated the effects on sperm parameters of the administration of a transdermal formulation of T gel for 3 months. The treated patients showed a significantly increased percentage of spermatozoa with normal form and progressive motility (p < .05 vs baseline), a significant reduction of CD45pos leucocytes in the semen (p < .05 vs baseline) and finally a significant increase of the seminal concentrations of zinc, fructose and alpha-glucosidase (p < .05 vs baseline) identified as key parameters associated to secretory function of the Male Accessory Glands. The results of this study suggest the use of transdermal T in hypogonadal patients with MAGI for favourable effects on sperm parameters.
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Antioxidants in Male Accessory Gland Infection
Antioxidants in Andrology, 2016Co-Authors: Aldo E. Calogero, Rosita A. Condorelli, Sandro La VigneraAbstract:Male Accessory Gland infections (MAGI) have a negative impact on the secretory function of the Male Accessory Glands, hence altering seminal fluid. MAGI can be classified according to the presence/absence of microorganisms in microbial forms, when it is possible to identify the presence of microorganisms, and in inflammatory forms, characterized by leukocytospermia and/or overproduction of reactive oxygen species (ROS). The treatment of choice of the microbial forms of MAGI is based on antibiotic administration, followed by anti-inflammatory drugs and antioxidants. The inflammatory forms, characterized by leukocytospermia and ROS overproduction, are mainly treated with anti-inflammatory drugs followed by antioxidants. Antioxidants may be prescribed both to ameliorate the symptoms of MAGI and to reduce sperm oxidative stress-induced damage that can lead to Male infertility.
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Hormonal treatment with transdermal testosterone in patients with Male Accessory Gland inflammation (MAGI): Effects on sperm parameters.
Andrologia, 2016Co-Authors: Sandro La Vignera, Rosita A. Condorelli, Laura M. Mongioì, S G Vitale, Giovanna Russo, Antonio Simone Laganà, Aldo E. CalogeroAbstract:Summary Recently, it has been reported that treatment with testosterone (T) could have favourable effects on prostate inflammation; however, the data appear inconsistent. The main evidences concern experimental studies, and there is lower information obtainable from clinical studies. This study was conducted on patients with diagnosis of Male Accessory Gland infection (MAGI) and a concomitant hormonal condition of acquired hypergonadotropic hypogonadism and has evaluated the effects on sperm parameters of the administration of a transdermal formulation of T gel for 3 months. The treated patients showed a significantly increased percentage of spermatozoa with normal form and progressive motility (p
Enzo Vicari - One of the best experts on this subject based on the ideXlab platform.
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acquired premature ejaculation and Male Accessory Gland infection relevance of ultrasound examination
Asian Journal of Andrology, 2016Co-Authors: Sandro La Vignera, Rosita A. Condorelli, Enzo Vicari, Vincenzo Favilla, Giuseppe Morgia, Aldo E. CalogeroAbstract:We have previously demonstrated a high frequency of premature ejaculation (PE) among patients with Male Accessory Gland infection (MAGI). The aim of this study was to evaluate the ultrasound (US) features of patients with MAGI and acquired premature ejaculation (APE) associated (MAGI-APEpos). US evaluation of 50 MAGI-APEpos patients compared to 50 patients with MAGI without PE (MAGI-PEneg) which represent the control group. The diagnosis of APE was made through the evaluation of Intravaginal ejaculation latency time (IELT) and confirmed with the questionnaire PEDT (Premature Ejaculation Diagnostic Tool). The main outcome measure was represented by the frequency of US criteria suggestive of P (prostatitis), V (vesiculitis), and E (epididymitis) in MAGI-APEpos and MAGI-PEneg patients. MAGI-APEpos patients showed a total number of US criteria significantly higher compared to MAGI-PEneg patients. MAGI-APEpos showed a higher frequency of US criteria of V and E (complicated forms of MAGI). Finally, in MAGI-APEpos group, it was found a positive relationship between the anteroposterior diameter (APD) of the caudal tract of the epididymis and the APD of the seminal vesicles, as well as between both diameters and the PEDT score. MAGI-APEpos patients have a peculiar US characterization compared to MAGI-PEneg patients. According to these results, US evaluation of the epididymal and of the prostato vesicular tract should be considered in the practical clinical approach of patients with MAGI and APE. In particular, it could be a support for a possible pathophysiological interpretation of this clinical problem in these patients.
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prevalence of human papilloma virus infection in patients with Male Accessory Gland infection
Reproductive Biomedicine Online, 2015Co-Authors: Sandro La Vignera, Rosita A. Condorelli, Enzo Vicari, Giuseppe Morgia, C Franchina, Guido Scalia, Antonino Perino, Rosaria Schillaci, Aldo E. CalogeroAbstract:The frequency of human papillomavirus (HPV) infection in the semen of patients with Male Accessory Gland infection (MAGI) was evaluated. One hundred infertile patients with MAGI were classified into group A: patients with an inflammatory MAGI (n = 48) and group B: patients with a microbial form (n = 52). Healthy age-matched fertile men (34.0 ± 4.0 years) made up the control group (n = 20). Amplification of HPV DNA was carried out by HPV-HS Bio nested polymerase chain reaction for the detection of HPV DNA sequences within the L1 ORF. Ten patients in group A (20.8%) and 15 patients in group B (28.8%) had a HPV infection; two controls (10.0%) had HPV infection. Patients with MAGI had a significantly higher frequency of HPV infection compared with controls; patients with a microbial MAGI had significantly higher frequency of HPV infection compared with patients with an inflammatory form (both P < 0.05). Patients with MAGI and HPV had a slight, but significantly lower sperm progressive motility and normal morphology compared with patients with MAGI HPV-negative (P < 0.05). Elevated frequency of HPV infection occurred in patients with MAGI, suggesting that HPV should be investigated in the diagnostic work-up of these patients.
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Male Accessory Gland infection: relevance of serum total testosterone levels.
International journal of endocrinology, 2014Co-Authors: Rosita A. Condorelli, Aldo E. Calogero, Enzo Vicari, Vincenzo Favilla, Sebastiano Cimino, Giorgio Ivan Russo, Giuseppe Morgia, Sandro La VigneraAbstract:Aim of the present study was to evaluate the different ultrasound characterization of fertile symptomatic patients with MAGI (Male Accessory Gland infection) according to different serum concentrations of total T (TT). We analyzed the ultrasound and hormonal data of 200 patients aged between 24.0 and 67.0 years. Patients were divided into six groups according to the sextile distribution of TT. Patients with serum concentrations of TT l 3.6 ng mL−1 had a higher mean duration of symptoms compared to the other examined groups. Patients with serum concentrations of TT g 6.6 ng mL−1 showed a frequency of ultrasound criteria suggestive for bilateral form of prostatitis and prostate-vesiculo-epididymitis and significantly lower compared to the other examined groups. At multivariate logistic regression analysis adjusted for age and BMI, TT was an independent predictive factor of prostatovesiculitis (OR = 0.818 [95% CI: 0.675–0.992]; ) and prostate-vesiculo-epididymitis (OR = 0.714 [95% CI: 0.578–0.880]; ), which represent the main forms of complicated MAGI. The results of this study suggest that Male hypogonadism could be associated with a different ultrasound characterization of these patients.
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Male Accessory Gland inflammation prevalence in type 2 diabetic patients with symptoms possibly reflecting autonomic neuropathy
Asian Journal of Andrology, 2014Co-Authors: Rosita A. Condorelli, Aldo E. Calogero, Enzo Vicari, Sandro La VigneraAbstract:Male Accessory Gland inflammation or infection (MAGI) is a potentially underdiagnosed complication of type 2 diabetes (DM2); specifically, we reported in a recent study that the frequency of MAGI was 43% among DM2 patients. In previous studies, we have demonstrated that diabetic autonomic neuropathy (DAN) is associated with a peculiar ultrasound characterization of the seminal vesicles (SVs) in DM2 patients. The aim of the present study was to evaluate the frequency of MAGI in two different categories of DM2 patients (i.e. patients with and without symptoms that possibly reflect DAN) and the respective ultrasound characterizations. Sixty DM2 patients with a mean (± s.e.m.) age of 42.0 ± 6.0 years (range: 34-47 years) were classified according to the presence or the absence of symptoms that could possibly reflect DAN (group A: DM2 with symptoms possibly reflecting DAN, n = 28 patients and group B: DM2 without symptoms possibly reflecting DAN, n = 32 patients). The patients in Group A exhibited a significantly higher frequency of MAGI compared with those in group B patients (P < 0.05); moreover, the Group A patients exhibited a significantly higher frequency of ultrasound signs suggestive of vesiculitis (P < 0.05). Finally, the concentrations of lymphocytes but not the concentrations of the leukocytes in the semen were significantly higher (P < 0.05) in group A compared with group B.
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prevalence of Male Accessory Gland inflammations infections in patients with type 2 diabetes mellitus
Journal of Endocrinological Investigation, 2013Co-Authors: Rosita A. Condorelli, Aldo E. Calogero, Enzo Vicari, Ylenia Duca, Vincenzo Favilla, Sebastiano Cimino, Giuseppe Morgia, M Di Mauro, Sandro La VigneraAbstract:Background: MAGI is an acronym that identifies the “Male Accessory Gland inflammations/infections”, a potential cause of Male infertility. Type 2 diabetes mellitus (DM2) prevalence is going to increase among men of reproductive age. Due to the high prevalence of these two conditions, we could suppose that they might appear together in the same patient. Aim: To evaluate MAGI prevalence in patients with DM2 in fertile age. Subjects and methods: A cross-sectional study carried out on patients with DM2 of fertile age. All patients underwent andrological evaluation for the identification of conventional MAGI diagnostic criteria. Results: DM2 patients showed a frequency of MAGI about 43%, significantly lower (p<0.05) than in infertile patients of the same age without diabetes, which showed a MAGI overall frequency of 61%. Among examined diabetic patients the prevalence of MAGI did not significantly differ between patients attending for diabetes care problems (glycemic control) and patients with andrological disorders. Finally, no significant difference in seminal inflammatory signs frequency was detected between patients with DM2 and infertile patients without diabetes. Finally, the correlation analysis showed a significant direct correlation between duration of diabetes and glycemic control with the prevalence of MAGI. Conclusion: MAGI prevalence in DM2 is lower than the one detected in age-matched infertile non-diabetic patients, however, as in infertile patients, there is a high frequency of seminal inflammatory signs. Moreover, the observed prevalence among diabetic patients with diabetes care problems and diabetic patients with andrological problems is not statistically different.
Mariana F. Wolfner - One of the best experts on this subject based on the ideXlab platform.
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the feMale post mating response requires genes expressed in the secondary cells of the Male Accessory Gland in drosophila melanogaster
Genetics, 2016Co-Authors: Jessica L Sitnik, Dragan Gligorov, Robert K Maeda, Francois Karch, Mariana F. WolfnerAbstract:Seminal proteins from the Drosophila Male Accessory Gland induce post-mating responses (PMR) in feMales. The PMR comprise behavioral and physiological changes that include increased egg laying, decreased receptivity to courting Males, and changes in the storage and use of sperm. Many of these changes are induced by a "sex peptide" (SP) and are maintained by SP's binding to, and slow release from, sperm. The Accessory Gland contains two secretory cell types with distinct morphological and developmental characteristics. Products of these "main" and "secondary" cells work interdependently to induce and maintain the PMR. To identify individual genes needed for the morphology and function of secondary cells, we studied iab-6(cocu) Males, whose secondary cells have abnormal morphology and fail to provide products to maintain the PMR. By RNA-seq, we identified 77 genes that are downregulated by a factor of >5× in iab-6(cocu) Males. By functional assays and microscopy, we tested 20 candidate genes and found that at least 9 are required for normal storage and release of SP in mated feMales. Knockdown of each of these 9 genes consequently leads to a reduction in egg laying and an increase in receptivity over time, confirming a role for the secondary cells in maintaining the long-term PMR. Interestingly, only 1 of the 9 genes, CG3349, encodes a previously reported seminal fluid protein (Sfp), suggesting that secondary cells may perform essential functions beyond the production and modification of known Sfps. At least 3 of the 9 genes also regulate the size and/or abundance of secondary cell vacuoles, suggesting that the vacuoles' contents may be important for the machinery used to maintain the PMR.
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induction of excessive endoplasmic reticulum stress in the drosophila Male Accessory Gland results in infertility
PLOS ONE, 2015Co-Authors: Clement Y Chow, Andrew G Clark, Frank W Avila, Mariana F. WolfnerAbstract:Endoplasmic reticulum (ER) stress occurs when misfolded proteins accumulate in the lumen of the ER. A cell responds to ER stress with the unfolded protein response (UPR), a complex program of transcriptional and translational changes aimed at clearing misfolded proteins. Secretory tissues and cells are particularly well adapted to respond to ER stress because their function requires high protein production and secretory load. The insect Male Accessory Gland (AG) is a secretory tissue involved in Male fertility. The AG secretes many seminal fluid proteins (SFPs) essential for Male reproduction. Among adult Drosophila tissues, we find that genes upregulated by ER stress are most highly expressed in the AG, suggesting that the AG is already undergoing high levels of ER stress due to its normal secretory functions. We hypothesized that induction of excessive ER stress in the AG above basal levels, would perturb normal function and provide a genetic tool for studying AG and SFP biology. To test this, we genetically induced excessive ER stress in the AG by conditional 1) expression of a misfolded protein or 2) knockdown of the UPR regulatory protein, BiP. Both genetic manipulations induced excessive ER stress in the AG, as indicated by the increase in Xbp1 splicing, a marker of ER stress. Both models resulted in a large decrease in or loss of SFP production and Male infertility. Sperm production, motility, and transfer appeared unaffected. The induction of strong ER stress in the insect Male AG may provide a simple way for studying or manipulating Male fertility, as it eliminates AG function while preserving sperm production.
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a novel function for the hox gene abd b in the Male Accessory Gland regulates the long term feMale post mating response in drosophila
PLOS Genetics, 2013Co-Authors: Dragan Gligorov, Mariana F. Wolfner, Jessica L Sitnik, Robert K Maeda, Francois KarchAbstract:In insects, products of the Male reproductive tract are essential for initiating and maintaining the feMale post-mating response (PMR). The PMR includes changes in egg laying, receptivity to courting Males, and sperm storage. In Drosophila, previous studies have determined that the main cells of the Male Accessory Gland produce some of the products required for these processes. However, nothing was known about the contribution of the Gland's other secretory cell type, the secondary cells. In the course of investigating the late functions of the homeotic gene, Abdominal-B (Abd-B), we discovered that Abd-B is specifically expressed in the secondary cells of the Drosophila Male Accessory Gland. Using an Abd-B BAC reporter coupled with a collection of genetic deletions, we discovered an enhancer from the iab-6 regulatory domain that is responsible for Abd-B expression in these cells and that apparently works independently from the segmentally regulated chromatin domains of the bithorax complex. Removal of this enhancer results in visible morphological defects in the secondary cells. We determined that mates of iab-6 mutant Males show defects in long-term egg laying and suppression of receptivity, and that products of the secondary cells are influential during sperm competition. Many of these phenotypes seem to be caused by a defect in the storage and gradual release of sex peptide in feMale mates of iab-6 mutant Males. We also found that Abd-B expression in the secondary cells contributes to glycosylation of at least three Accessory Gland proteins: ovulin (Acp26Aa), CG1656, and CG1652. Our results demonstrate that long-term post-mating changes observed in mated feMales are not solely induced by main cell secretions, as previously believed, but that secondary cells also play an important role in Male fertility by extending the feMale PMR. Overall, these discoveries provide new insights into how these two cell types cooperate to produce and maintain a robust feMale PMR.
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cross species comparison of drosophila Male Accessory Gland protein genes
Genetics, 2005Co-Authors: Jacob L Mueller, Ravi K Ram, Lisa A Mcgraw, M Bloch C Qazi, Eric D Siggia, Andrew G Clark, Charles F Aquadro, Mariana F. WolfnerAbstract:Drosophila melanogaster Males transfer seminal fluid proteins along with sperm during mating. Among these proteins, ACPs ( Ac cessory Gland p roteins) from the Male9s Accessory Gland induce behavioral, physiological, and life span reduction in mated feMales and mediate sperm storage and utilization. A previous evolutionary EST screen in D. simulans identified partial cDNAs for 57 new candidate ACPs. Here we report the annotation and confirmation of the corresponding Acp genes in D. melanogaster . Of 57 new candidate Acp genes previously reported in D. melanogaster , 34 conform to our more stringent criteria for encoding putative Male Accessory Gland extracellular proteins, thus bringing the total number of ACPs identified to 52 (34 plus 18 previously identified). This comprehensive set of Acp genes allows us to dissect the patterns of evolutionary change in a suite of proteins from a single Male-specific reproductive tissue. We used sequence-based analysis to examine codon bias, gene duplications, and levels of divergence (via d N / d S values and ortholog detection) of the 52 D. melanogaster ACPs in D. simulans , D. yakuba , and D. pseudoobscura . We show that 58% of the 52 D. melanogaster Acp genes are detectable in D. pseudoobscura . Sequence comparisons of ACPs shared and not shared between D. melanogaster and D. pseudoobscura show that there are separate classes undergoing distinctly dissimilar evolutionary dynamics.
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Fates and targets of Male Accessory Gland proteins in mated feMale Drosophila melanogaster.
Insect biochemistry and molecular biology, 2005Co-Authors: K. Ravi Ram, Mariana F. WolfnerAbstract:Male Accessory Gland proteins (Acps) in Drosophila are components of the seminal fluid and are transferred to feMales during copulation. In mated feMales, Acps enhance egg production, augment sperm storage, induce refractory mating behaviors, and affect the feMale's longevity. To address the functions of eight previously uncharacterized Acps and further analyze five others, we determined the tissues to which they target after transfer to feMales. Each Acp has multiple targets and is unique in its pattern of localization. Within the reproductive tract, Acps target to the uterus, oviduct, sperm storage organs, ovary and oocytes. Some Acps also leave the reproductive tract, to enter the hemolymph. Some Acps are detected on the surface of eggs laid by mated feMales but were not detectable within those eggs. Our results can help to identify the likely functions of these Acps as well as to create models for the mechanism of action of Acps.