The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
Peter Timms - One of the best experts on this subject based on the ideXlab platform.
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ureaplasma parvum and ureaplasma urealyticum are detected in Semen after washing before assisted reproductive technology procedures
Fertility and Sterility, 2003Co-Authors: Christine L Knox, John A Allan, Janet M Allan, Rohini W Edirisinghe, Deborah J Stenzel, Felicity Lawrence, David M Purdie, Peter TimmsAbstract:Abstract Objective: To investigate the prevalence of ureaplasmas in Semen and washed Semen and to explore their effect on Semen andrology variables. Design: Prospective study. Setting: In vitro fertilization (IVF) unit of a private hospital. Patient(s): Three hundred forty-three men participating in an assisted reproductive technology (ART) treatment cycle. Main outcome measure(s) The prevalence of ureaplasmas in Semen and washed Semen tested by culture, polymerase chain reaction assays, and indirect immunofluorescent antibody assays. Result(s): Ureaplasmas were detected in 73 of 343 (22%) Semen samples and 29 of 343 (8.5%) washed Semen samples. Ureaplasmas adherent to the surface of spermatozoa were demonstrated by indirect immunofluorescent antibody testing. Ureplasma parvum serovar 6 (36.6%) and U. urealyticum (30%) were the most prevalent isolates in washed Semen. A comparison of the Semen andrology variables of washed Semen ureaplasma positive and negative groups demonstrated a lower proportion of nonmotile sperm in men ureaplasma positive for washed Semen. Conclusion(s): Ureaplasmas are not always removed from Semen by a standard ART washing procedure and can remain adherent to the surface of spermatozoa.
Weibo Liang - One of the best experts on this subject based on the ideXlab platform.
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Semen-specific miRNAs: Suitable for the distinction of infertile Semen in the body fluid identification?
Forensic science international. Genetics, 2017Co-Authors: Huan Tian, Duo Peng, Yu Tan, Hui Wang, Weibo LiangAbstract:Abstract Non-protein coding RNA, miRNAs (microRNAs), are a class of promising molecular biomarkers for forensic body fluid identification (BFI) as their small size and tissue-specific expression manners. A number of studies have shown that Semen can be distinguished from forensic-related body fluids (such as menstrual blood, venous blood, vaginal fluid, saliva, etc.) using Semen-specific miRNAs through microassay screening and RT-qRCR. Infertility is becoming a global health problem, affecting 10%-15% of couples worldwide, and half of the cases are the result of male factors (Lian et al., 2009 [ 1 ]). Forensic researchers have to consider the impact of Semen infertility on Semen identification with a high incidence of infertility. In the present study, normal Semen (NS) and four other types of infertile Semen samples, including asthenospermia (AS), oligospermia (OS), azoospermia (AZ), oligospermia and asthenospermia (OSAS) Semen, were collected. The expression levels of a set of Semen-specific miRNA markers (miR-10a, miR-10b, miR-135a, miR-135b, miR-888 and miR-891a) were evaluated using a real-time quantitative PCR technique with a specific fluorescence-labelled TaqMan probe. The results showed the significantly high expression of these miRNAs in normal Semen, and the molecules have Semen specificity. Nevertheless, a distinct down-regulation in the expression of infertile samples compared with normal Semen samples was observed. Moreover, differences in the results of selected optimal biomarkers between the discriminant function and two-dimensional scatter plots were also detected. The goal of the present study was to identify a small set of Semen-specific miRNAs that efficiently and accurately distinguish Semen (fertile and infertile) from other forensic-related body fluids. The results of the present study suggest that attention should be paid to infertile Semen samples when using miRNAs to identify Semen samples, for which would have a far-reaching impact on forensic identification.
Brian Mckeon - One of the best experts on this subject based on the ideXlab platform.
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human immunodeficiency virus type 1 shedding pattern in Semen correlates with the compartmentalization of viral quasi species between blood and Semen
The Journal of Infectious Diseases, 2000Co-Authors: Phalguni Gupta, Caroline Leroux, Bruce K Patterson, Lawrence A Kingsley, Charles R Rinaldo, Ming Ding, Yue Chen, Kathy Kulka, William Buchanan, Brian MckeonAbstract:: High levels of human immunodeficiency virus (HIV) type 1 have been detected in Semen at all stages of disease. However, it is not clear whether HIV-1 is shed in Semen continuously or intermittently. In a prospective longitudinal study, viral RNA was measured weekly for 10 weeks in Semen and blood of HIV-seropositive subjects. Results showed three different patterns of HIV-1 shedding in Semen: none (28%), continuous (28%), and intermittent (44%). In contrast, there was no change in blood plasma virus load during the study period. Phylogenetic analysis of the envelope sequences of HIV-1 RNA in Semen and blood revealed distinct virus populations in Semen and blood of intermittent shedders but similar virus populations in the Semen and blood of continuous shedder. These results indicate for the first time that HIV-1 is shed primarily in an intermittent manner and that shedding patterns of HIV-1 in Semen are related to compartmentalization of HIV-1 between Semen and blood.
Christine L Knox - One of the best experts on this subject based on the ideXlab platform.
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ureaplasma parvum and ureaplasma urealyticum are detected in Semen after washing before assisted reproductive technology procedures
Fertility and Sterility, 2003Co-Authors: Christine L Knox, John A Allan, Janet M Allan, Rohini W Edirisinghe, Deborah J Stenzel, Felicity Lawrence, David M Purdie, Peter TimmsAbstract:Abstract Objective: To investigate the prevalence of ureaplasmas in Semen and washed Semen and to explore their effect on Semen andrology variables. Design: Prospective study. Setting: In vitro fertilization (IVF) unit of a private hospital. Patient(s): Three hundred forty-three men participating in an assisted reproductive technology (ART) treatment cycle. Main outcome measure(s) The prevalence of ureaplasmas in Semen and washed Semen tested by culture, polymerase chain reaction assays, and indirect immunofluorescent antibody assays. Result(s): Ureaplasmas were detected in 73 of 343 (22%) Semen samples and 29 of 343 (8.5%) washed Semen samples. Ureaplasmas adherent to the surface of spermatozoa were demonstrated by indirect immunofluorescent antibody testing. Ureplasma parvum serovar 6 (36.6%) and U. urealyticum (30%) were the most prevalent isolates in washed Semen. A comparison of the Semen andrology variables of washed Semen ureaplasma positive and negative groups demonstrated a lower proportion of nonmotile sperm in men ureaplasma positive for washed Semen. Conclusion(s): Ureaplasmas are not always removed from Semen by a standard ART washing procedure and can remain adherent to the surface of spermatozoa.
Huan Tian - One of the best experts on this subject based on the ideXlab platform.
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Semen-specific miRNAs: Suitable for the distinction of infertile Semen in the body fluid identification?
Forensic science international. Genetics, 2017Co-Authors: Huan Tian, Duo Peng, Yu Tan, Hui Wang, Weibo LiangAbstract:Abstract Non-protein coding RNA, miRNAs (microRNAs), are a class of promising molecular biomarkers for forensic body fluid identification (BFI) as their small size and tissue-specific expression manners. A number of studies have shown that Semen can be distinguished from forensic-related body fluids (such as menstrual blood, venous blood, vaginal fluid, saliva, etc.) using Semen-specific miRNAs through microassay screening and RT-qRCR. Infertility is becoming a global health problem, affecting 10%-15% of couples worldwide, and half of the cases are the result of male factors (Lian et al., 2009 [ 1 ]). Forensic researchers have to consider the impact of Semen infertility on Semen identification with a high incidence of infertility. In the present study, normal Semen (NS) and four other types of infertile Semen samples, including asthenospermia (AS), oligospermia (OS), azoospermia (AZ), oligospermia and asthenospermia (OSAS) Semen, were collected. The expression levels of a set of Semen-specific miRNA markers (miR-10a, miR-10b, miR-135a, miR-135b, miR-888 and miR-891a) were evaluated using a real-time quantitative PCR technique with a specific fluorescence-labelled TaqMan probe. The results showed the significantly high expression of these miRNAs in normal Semen, and the molecules have Semen specificity. Nevertheless, a distinct down-regulation in the expression of infertile samples compared with normal Semen samples was observed. Moreover, differences in the results of selected optimal biomarkers between the discriminant function and two-dimensional scatter plots were also detected. The goal of the present study was to identify a small set of Semen-specific miRNAs that efficiently and accurately distinguish Semen (fertile and infertile) from other forensic-related body fluids. The results of the present study suggest that attention should be paid to infertile Semen samples when using miRNAs to identify Semen samples, for which would have a far-reaching impact on forensic identification.
