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Jansen Fernandes Medeiros - One of the best experts on this subject based on the ideXlab platform.
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sensitivity of diagnostic methods for Mansonella ozzardi microfilariae detection in the brazilian amazon region
Memorias Do Instituto Oswaldo Cruz, 2018Co-Authors: Jansen Fernandes Medeiros, Felipe Arley Costa Pessoa, Jacob Moyses Cohen, Gilberto Fontes, Edmar Vaz De Andrade, Vilma Lopes Do Nascimento, Moreno S Rodrigues, Marilaine MartinsAbstract:BACKGROUND The human filarial worm Mansonella ozzardi is highly endemic in the large tributaries of the Amazon River. This infection is still highly neglected and can be falsely negative when microfilariae levels are low. OBJECTIVES This study investigated the frequency of individuals with M. ozzardi in riverine communities in Coari municipality, Brazilian Amazon. METHODS Different diagnostic methods including polymerase chain reaction (PCR), blood polycarbonate membrane filtration (PCMF), Knott's method (Knott), digital thick blood smears (DTBS) and venous thick blood smears (VTBS) were used to compare sensitivity and specificity among the methods. Data were analysed using PCMF and Bayesian latent class models (BLCM) as the gold standard. We used BLCM to calculate the prevalence of mansonelliasis based on the results of five diagnostic methods. FINDINGS The prevalence of mansonelliasis was 35.4% by PCMF and 30.1% by BLCM. PCR and Knott methods both possessed high sensitivity. Sensitivity relative to PCMF was 98.5% [95% confidence interval (CI): 92.0 - 99.7] for PCR and 83.5% (95% CI: 72.9 - 90.5) for Knott. Sensitivity derived by BLCM was 100% (95% CI 93.7 - 100) for PCMF, 100% (95% CI: 93.7 - 100) for PCR and 98.3% (95% CI: 90.6 - 99.9) for Knott. The odds ratio of being diagnosed as microfilaremic increased with age but did not differ between genders. Microfilariae loads were higher in subjects aged 30 - 45 and 45 - 60 years. MAIN CONCLUSIONS PCMF and PCR were the best methods to assess the prevalence of mansonelliasis in our samples. As such, using these methods could lead to higher prevalence of mansonelliasis in this region than the most commonly used method (i.e., thick blood smears).
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phase iii clinical trial to evaluate ivermectin in the reduction of Mansonella ozzardi infection in the brazilian amazon
American Journal of Tropical Medicine and Hygiene, 2018Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Gilberto Fontes, Ricardo De Godoi Mattos Ferreira, Juliana De Souza Almeida Aranha Camargo, Antonieta Relvas Pereira, Mayara Costa De Oliveira Laudisse, Luis Marcelo Aranha CamargoAbstract:The treatment of mansonelliasis is still a challenge because there are few clinical trials for the treatment of the disease. This double-blind, randomized, placebo-controlled study (phase III clinical trial) was conducted to evaluate the effectiveness of a single oral dose of ivermectin (0.15 mg/kg) in the reduction of the Mansonella ozzardi microfilaraemia and the occurrence of adverse effects in infected people compared with the control group treated with placebo. A total of 49 microfilaraemic patients were randomly selected from the municipality of Labrea, State of Amazonas, in the Brazilian Amazon. Among them, 40 patients have concluded the study, 19 treated with ivermectin and 21 treated with placebo. In the first and third days after the treatment, all the patients were clinically evaluated, and the diagnostic and quantification of blood microfilariae through blood filtration in polycarbonate membranes was performed. A significant reduction of the microfilaraemia (99.9%) was observed in the patients who received ivermectin. Slight changes in laboratory test results, without clinical importance, were seen in treated and control groups. Our results suggest that ivermectin is effective and safe for the treatment of infections caused by M. ozzardi.
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immune response in Mansonella ozzardi infection modulated by il 6 il 10 axis in amazon region of brazil
Cytokine, 2017Co-Authors: Allyson Guimaraes Costa, Felipe Arley Costa Pessoa, Aya Sadahiro, Andrea Monteiro Tarrago, Bruna Pires Loiola, Adriana Malheiro, Jansen Fernandes MedeirosAbstract:Mansonellosis is an endemic disease in the South and Central America. In Brazil, one of the etiological agents is Mansonella ozzardi. This filarial infection is yet poorly understood, with a controversial morbity, presenting since a oligosymptoms, malaria-like signs or without complaint in humans. The knowledge of the human immune response to microfilariae infection is limited mainly by different evolutionary cycles of the parasite in the host. In addition, the prevalence of this filarial parasite infection is high in several regions of Amazonas State. A cross-sectional study was conducted in an endemic area for microfilariae of M. ozzardi (MF) infection in the Amazonas State, Brazil. Proinflammatory and regulatory cytokines (IL-2, IL-4, IL-6, IL-10, TNF, IFN-gamma, and IL-17A) were measured in cryopreserved serum using the Cytometric Bead Array techniques (CBA) in 54 patients diagnosed with M. ozzardi infection and 55 individuals without the infection were included in the study (Controls). The IL-4, IL-6 and IL-10 level increased in infected patients with MF infection, while IL-17A increased in control only. When we compared controls to patients with high or low parasite load, the increased level of IL-6 and IL-10 were maintained. IL-6 contributes to the proinflammatory activity and IL-10 modulates Th1, Th2 and Th17 immune response. Furthermore, IL-4 was detected as a marker in the MF infection and MF patients with low parasite load, indicating the action of the Th2 cell response. The complex network of cytokines acting during M. ozzardi infection depends on a fine balance to determine a host protective effect or filarial persistence. Therefore, these results suggest that the immune response in MF infection is modulated by IL-6/IL-10 axis.
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occurrence of Mansonella ozzardi diagnosed using a polycarbonate membrane in a riverside population of labrea in the western brazilian amazon
Revista Da Sociedade Brasileira De Medicina Tropical, 2016Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Luana Janaina Souza Vera, Gilberto Fontes, Gabriel De Deus Vieira, Juliana Souza De Almeida Aranha Camargo, Ricardo De Godoi Mattos Ferreira, Luis Marcelo Aranha CamargoAbstract:INTRODUCTION Mansonella ozzardi is a widely distributed filaria worm in the Amazon region. This study aimed to determine the prevalence of M. ozzardi infection in riverine communities of Labrea municipality, Amazonas State, Brazil. METHODS A diagnostic blood filtration method in a polycarbonate membrane was used. RESULTS M. ozzardi was found in 50.3% of the sample, with the highest prevalence in farmers/fishermen (69.4%; χ 2 = -19.14, p<0.001). The prevalence was higher in longer-term residents (≥11 years; 60.2%). CONCLUSIONS M. ozzardi infection rates are high near the Purus River, much greater than those previously reported based on diagnosis using thick blood smears.
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Occurrence of Mansonella ozzardi diagnosed using a polycarbonate membrane in a riverside population of Lábrea in the Western Brazilian Amazon
Sociedade Brasileira de Medicina Tropical (SBMT), 2016Co-Authors: Jansen Fernandes Medeiros, Sergio De Almeida Basano, Luana Janaina Souza Vera, Gilberto Fontes, Gabriel De Deus Vieira, Juliana Souza De Almeida Aranha Camargo, Ricardo De Godoi Mattos Ferreira, Luis Marcelo Aranha CamargoAbstract:Abstract: INTRODUCTION Mansonella ozzardi is a widely distributed filaria worm in the Amazon region. This study aimed to determine the prevalence of M. ozzardi infection in riverine communities of Lábrea municipality, Amazonas State, Brazil. METHODS A diagnostic blood filtration method in a polycarbonate membrane was used. RESULTS M. ozzardi was found in 50.3% of the sample, with the highest prevalence in farmers/fishermen (69.4%; χ 2 = -19.14, p
Luis Marcelo Aranha Camargo - One of the best experts on this subject based on the ideXlab platform.
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phase iii clinical trial to evaluate ivermectin in the reduction of Mansonella ozzardi infection in the brazilian amazon
American Journal of Tropical Medicine and Hygiene, 2018Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Gilberto Fontes, Ricardo De Godoi Mattos Ferreira, Juliana De Souza Almeida Aranha Camargo, Antonieta Relvas Pereira, Mayara Costa De Oliveira Laudisse, Luis Marcelo Aranha CamargoAbstract:The treatment of mansonelliasis is still a challenge because there are few clinical trials for the treatment of the disease. This double-blind, randomized, placebo-controlled study (phase III clinical trial) was conducted to evaluate the effectiveness of a single oral dose of ivermectin (0.15 mg/kg) in the reduction of the Mansonella ozzardi microfilaraemia and the occurrence of adverse effects in infected people compared with the control group treated with placebo. A total of 49 microfilaraemic patients were randomly selected from the municipality of Labrea, State of Amazonas, in the Brazilian Amazon. Among them, 40 patients have concluded the study, 19 treated with ivermectin and 21 treated with placebo. In the first and third days after the treatment, all the patients were clinically evaluated, and the diagnostic and quantification of blood microfilariae through blood filtration in polycarbonate membranes was performed. A significant reduction of the microfilaraemia (99.9%) was observed in the patients who received ivermectin. Slight changes in laboratory test results, without clinical importance, were seen in treated and control groups. Our results suggest that ivermectin is effective and safe for the treatment of infections caused by M. ozzardi.
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occurrence of Mansonella ozzardi diagnosed using a polycarbonate membrane in a riverside population of labrea in the western brazilian amazon
Revista Da Sociedade Brasileira De Medicina Tropical, 2016Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Luana Janaina Souza Vera, Gilberto Fontes, Gabriel De Deus Vieira, Juliana Souza De Almeida Aranha Camargo, Ricardo De Godoi Mattos Ferreira, Luis Marcelo Aranha CamargoAbstract:INTRODUCTION Mansonella ozzardi is a widely distributed filaria worm in the Amazon region. This study aimed to determine the prevalence of M. ozzardi infection in riverine communities of Labrea municipality, Amazonas State, Brazil. METHODS A diagnostic blood filtration method in a polycarbonate membrane was used. RESULTS M. ozzardi was found in 50.3% of the sample, with the highest prevalence in farmers/fishermen (69.4%; χ 2 = -19.14, p<0.001). The prevalence was higher in longer-term residents (≥11 years; 60.2%). CONCLUSIONS M. ozzardi infection rates are high near the Purus River, much greater than those previously reported based on diagnosis using thick blood smears.
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Occurrence of Mansonella ozzardi diagnosed using a polycarbonate membrane in a riverside population of Lábrea in the Western Brazilian Amazon
Sociedade Brasileira de Medicina Tropical (SBMT), 2016Co-Authors: Jansen Fernandes Medeiros, Sergio De Almeida Basano, Luana Janaina Souza Vera, Gilberto Fontes, Gabriel De Deus Vieira, Juliana Souza De Almeida Aranha Camargo, Ricardo De Godoi Mattos Ferreira, Luis Marcelo Aranha CamargoAbstract:Abstract: INTRODUCTION Mansonella ozzardi is a widely distributed filaria worm in the Amazon region. This study aimed to determine the prevalence of M. ozzardi infection in riverine communities of Lábrea municipality, Amazonas State, Brazil. METHODS A diagnostic blood filtration method in a polycarbonate membrane was used. RESULTS M. ozzardi was found in 50.3% of the sample, with the highest prevalence in farmers/fishermen (69.4%; χ 2 = -19.14, p
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sustained clearance of Mansonella ozzardi infection after treatment with ivermectin in the brazilian amazon
American Journal of Tropical Medicine and Hygiene, 2014Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Luana Janaina Souza Vera, Gilberto Fontes, Juliana Souza De Almeida Aranha Camargo, Ricardo De Godoi Mattos Ferreira, Marcos Paulo Parente Araujo, Maira Santiago Pires Parente, Pedro Di Tarique Barreto Crispim, Luis Marcelo Aranha CamargoAbstract:Therapy for mansonelliasis is challenging because there is no standard drug recommended for its treatment. This non-randomized study was conducted to evaluate the effectiveness of a single dose of 0.15 mg/kg of ivermectin to reduce Mansonella ozzardi microfilaraemia in infected persons. A total of 74 patients were studied within the municipality of Labrea, which is located in Amazonas State, Brazil. The patients were treated with ivermectin after detection of the parasite by blood examination. Significant microfilaraemia reduction was observed and its residual effect was maintained for at least 12 months. There was no significant change in the laboratory blood count, hepatic metabolites, and nitrogen-bounding compound excreta dosage values that could compromise the use of this drug, demonstrating that ivermectin has a low toxicity level.
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current title mansonelliasis in rondonia brazil investigation in the occurrence of Mansonella ozzardi in the state of rondonia western amazonia brazil mansonelose em rondonia brasil investigacao da ocorrencia da Mansonella ozzardi no estado de ro
Revista Da Sociedade Brasileira De Medicina Tropical, 2012Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Juliana Aranha Camargo, Luana Janaina Souza Vera, Suzane Neves Velasques, Guilherme Maerschner Ogawa, Gilberto Fontes, Luis Marcelo Aranha CamargoAbstract:Abstract Introduction: Mansonella ozzardi is a filaria, transmitted by Diptera Simuliidae flies (black fies), is widely spread in the Amazon, while the occurrence of mansonelliasis in the state of Rondonia has not been reported so far. The existence of such a p arasitic disease in the state was investigated. Methodology: 4,452 people were examined alongside the Rivers named Madeira, Mamore, Guapore, Machado, and Preto, in the state of Rondonia by means of the thick blood smears method. Alongside the rivers named Machado and Guapore 2,112 adult simuliids specimens were captured. Results: There has not been found infected people, although there has been found simuliids with vectorial potential as Cerqueirellum pydanielli, C. argentiscutum and C. amazonicum. Conclusion: Despite of the existence of flies with vectorial potential, and the proximity to areas of high prevalence of mansonelliasis in the Brazilian western Amazon territory, there has not been found people infected by this filariasis in the State of Rondonia. Keywords: Mansonella ozzardi – microfilariae – Simuliidae - Rondonia RESUMO Introducao: Mansonella ozzardi e uma filaria que tem como vetor dipteros simulideos e e amplamente distribuida na Amazonia. Nao ha informacoes sobre a ocorrencia da mansonelose em Rondonia. Procurou-se verificar a existencia da parasitose no estado. Metodologia: Foram examinadas 4.452 pessoas as margens dos rios Madeira, Mamore, Guapore, Machado e Preto em Rondonia, atraves da tecnica da gota espessa de sangue. Capturou-se as margens do rio Machado e Guapore 2.112 simulideos adultos das especies Cerqueirellum pydanieli e Chirostilbia pertinax. Resultados: Nao foram encontradas pessoas infectadas e foram encontrados simulideos com potencial de transmissao da parasitose. Conclusao: Apesar da existencia de vetores com potencial de transmissao e da proximidade com areas de alta prevalencia de mansonelose na Amazonia ocidental do Brasil, nao foram encontrados portadores desta filariose em Rondonia. Palavras-chaves: Mansonella ozzardi – microfilarias – Simuliidae - Rondonia
Sergio De Almeida Basano - One of the best experts on this subject based on the ideXlab platform.
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phase iii clinical trial to evaluate ivermectin in the reduction of Mansonella ozzardi infection in the brazilian amazon
American Journal of Tropical Medicine and Hygiene, 2018Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Gilberto Fontes, Ricardo De Godoi Mattos Ferreira, Juliana De Souza Almeida Aranha Camargo, Antonieta Relvas Pereira, Mayara Costa De Oliveira Laudisse, Luis Marcelo Aranha CamargoAbstract:The treatment of mansonelliasis is still a challenge because there are few clinical trials for the treatment of the disease. This double-blind, randomized, placebo-controlled study (phase III clinical trial) was conducted to evaluate the effectiveness of a single oral dose of ivermectin (0.15 mg/kg) in the reduction of the Mansonella ozzardi microfilaraemia and the occurrence of adverse effects in infected people compared with the control group treated with placebo. A total of 49 microfilaraemic patients were randomly selected from the municipality of Labrea, State of Amazonas, in the Brazilian Amazon. Among them, 40 patients have concluded the study, 19 treated with ivermectin and 21 treated with placebo. In the first and third days after the treatment, all the patients were clinically evaluated, and the diagnostic and quantification of blood microfilariae through blood filtration in polycarbonate membranes was performed. A significant reduction of the microfilaraemia (99.9%) was observed in the patients who received ivermectin. Slight changes in laboratory test results, without clinical importance, were seen in treated and control groups. Our results suggest that ivermectin is effective and safe for the treatment of infections caused by M. ozzardi.
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Preliminary study of the reduction of microfilaremia in vivo of Mansonella ozzardi (Manson, 1897) with use of ivermectin, using the blood filtration polycarbonate membrane technique, in Labrea, Brazilian Amazon, Western Amazon, Brazil.
Universidade de São Paulo, 2016Co-Authors: Sergio De Almeida BasanoAbstract:Estudo preliminar da redução da microfilaremia in vivo de Mansonella ozzardi (Manson, 1897) com uso de ivermectina, utilizando a técnica de filtração em membrana de policarbonato, Lábrea, Amazonas, Brasil. Estudou-se a eficácia do uso de dose única de ivermectina 0,15 mg/kg de peso no tratamento de 74 pacientes com infecção por Mansonella ozzardi. Inicialmente foi realizado a coleta de sangue para o diagnóstico parasitológico utilizando a filtração de sangue em membrana de policarbonato e para análises bioquímicas e hematológicas. Foi realizado a quantificação de microfilárias antes e depois do tratamento seguindo-os até 1 ano. Foi observado uma redução estatisticamente significativa em relação à densidade de microfilárias (χ2 de Friedman = 159,00; valor-p < 0, 0001) após 1 ano do uso da medicação, e que não houve alterações laboratoriais e efeitos adversos que comprometessem o uso da ivermectina. Concluiu-se que o fármaco nesta dose é eficaz e seguro, e mantém o \"clearance\" de microfilaremia pelo menos 1 ano após o uso.Preliminary study of the reduction of microfilaremia in vivo of Mansonella ozzardi (Manson, 1897) with use of ivermectin, using the blood filtration polycarbonate membrane technique, in Labrea, Brazilian Amazon, Brazil. The study focused in the efficacy and tolerability of a single-dose use of ivermectin 0.15 mg / kg body weight in the treatment of 74 patients infected by the filarial worm M. ozzardi. Before and after the parasitological diagnosis by a blood filtration polycarbonate membrane and treatment, anamnesis, clinical examination and blood collection for quantification of microfilariae, biochemical and hematological analysis were done, comparing the outcomes of patients the in first day and after 72 hours and at least following up the patients for 1 year. The study concluded that there was a statistically significant reduction in microfilariaemia density (χ2 Friedman = 159, 00; p-value
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occurrence of Mansonella ozzardi diagnosed using a polycarbonate membrane in a riverside population of labrea in the western brazilian amazon
Revista Da Sociedade Brasileira De Medicina Tropical, 2016Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Luana Janaina Souza Vera, Gilberto Fontes, Gabriel De Deus Vieira, Juliana Souza De Almeida Aranha Camargo, Ricardo De Godoi Mattos Ferreira, Luis Marcelo Aranha CamargoAbstract:INTRODUCTION Mansonella ozzardi is a widely distributed filaria worm in the Amazon region. This study aimed to determine the prevalence of M. ozzardi infection in riverine communities of Labrea municipality, Amazonas State, Brazil. METHODS A diagnostic blood filtration method in a polycarbonate membrane was used. RESULTS M. ozzardi was found in 50.3% of the sample, with the highest prevalence in farmers/fishermen (69.4%; χ 2 = -19.14, p<0.001). The prevalence was higher in longer-term residents (≥11 years; 60.2%). CONCLUSIONS M. ozzardi infection rates are high near the Purus River, much greater than those previously reported based on diagnosis using thick blood smears.
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Occurrence of Mansonella ozzardi diagnosed using a polycarbonate membrane in a riverside population of Lábrea in the Western Brazilian Amazon
Sociedade Brasileira de Medicina Tropical (SBMT), 2016Co-Authors: Jansen Fernandes Medeiros, Sergio De Almeida Basano, Luana Janaina Souza Vera, Gilberto Fontes, Gabriel De Deus Vieira, Juliana Souza De Almeida Aranha Camargo, Ricardo De Godoi Mattos Ferreira, Luis Marcelo Aranha CamargoAbstract:Abstract: INTRODUCTION Mansonella ozzardi is a widely distributed filaria worm in the Amazon region. This study aimed to determine the prevalence of M. ozzardi infection in riverine communities of Lábrea municipality, Amazonas State, Brazil. METHODS A diagnostic blood filtration method in a polycarbonate membrane was used. RESULTS M. ozzardi was found in 50.3% of the sample, with the highest prevalence in farmers/fishermen (69.4%; χ 2 = -19.14, p
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sustained clearance of Mansonella ozzardi infection after treatment with ivermectin in the brazilian amazon
American Journal of Tropical Medicine and Hygiene, 2014Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Luana Janaina Souza Vera, Gilberto Fontes, Juliana Souza De Almeida Aranha Camargo, Ricardo De Godoi Mattos Ferreira, Marcos Paulo Parente Araujo, Maira Santiago Pires Parente, Pedro Di Tarique Barreto Crispim, Luis Marcelo Aranha CamargoAbstract:Therapy for mansonelliasis is challenging because there is no standard drug recommended for its treatment. This non-randomized study was conducted to evaluate the effectiveness of a single dose of 0.15 mg/kg of ivermectin to reduce Mansonella ozzardi microfilaraemia in infected persons. A total of 74 patients were studied within the municipality of Labrea, which is located in Amazonas State, Brazil. The patients were treated with ivermectin after detection of the parasite by blood examination. Significant microfilaraemia reduction was observed and its residual effect was maintained for at least 12 months. There was no significant change in the laboratory blood count, hepatic metabolites, and nitrogen-bounding compound excreta dosage values that could compromise the use of this drug, demonstrating that ivermectin has a low toxicity level.
Gilberto Fontes - One of the best experts on this subject based on the ideXlab platform.
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sensitivity of diagnostic methods for Mansonella ozzardi microfilariae detection in the brazilian amazon region
Memorias Do Instituto Oswaldo Cruz, 2018Co-Authors: Jansen Fernandes Medeiros, Felipe Arley Costa Pessoa, Jacob Moyses Cohen, Gilberto Fontes, Edmar Vaz De Andrade, Vilma Lopes Do Nascimento, Moreno S Rodrigues, Marilaine MartinsAbstract:BACKGROUND The human filarial worm Mansonella ozzardi is highly endemic in the large tributaries of the Amazon River. This infection is still highly neglected and can be falsely negative when microfilariae levels are low. OBJECTIVES This study investigated the frequency of individuals with M. ozzardi in riverine communities in Coari municipality, Brazilian Amazon. METHODS Different diagnostic methods including polymerase chain reaction (PCR), blood polycarbonate membrane filtration (PCMF), Knott's method (Knott), digital thick blood smears (DTBS) and venous thick blood smears (VTBS) were used to compare sensitivity and specificity among the methods. Data were analysed using PCMF and Bayesian latent class models (BLCM) as the gold standard. We used BLCM to calculate the prevalence of mansonelliasis based on the results of five diagnostic methods. FINDINGS The prevalence of mansonelliasis was 35.4% by PCMF and 30.1% by BLCM. PCR and Knott methods both possessed high sensitivity. Sensitivity relative to PCMF was 98.5% [95% confidence interval (CI): 92.0 - 99.7] for PCR and 83.5% (95% CI: 72.9 - 90.5) for Knott. Sensitivity derived by BLCM was 100% (95% CI 93.7 - 100) for PCMF, 100% (95% CI: 93.7 - 100) for PCR and 98.3% (95% CI: 90.6 - 99.9) for Knott. The odds ratio of being diagnosed as microfilaremic increased with age but did not differ between genders. Microfilariae loads were higher in subjects aged 30 - 45 and 45 - 60 years. MAIN CONCLUSIONS PCMF and PCR were the best methods to assess the prevalence of mansonelliasis in our samples. As such, using these methods could lead to higher prevalence of mansonelliasis in this region than the most commonly used method (i.e., thick blood smears).
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phase iii clinical trial to evaluate ivermectin in the reduction of Mansonella ozzardi infection in the brazilian amazon
American Journal of Tropical Medicine and Hygiene, 2018Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Gilberto Fontes, Ricardo De Godoi Mattos Ferreira, Juliana De Souza Almeida Aranha Camargo, Antonieta Relvas Pereira, Mayara Costa De Oliveira Laudisse, Luis Marcelo Aranha CamargoAbstract:The treatment of mansonelliasis is still a challenge because there are few clinical trials for the treatment of the disease. This double-blind, randomized, placebo-controlled study (phase III clinical trial) was conducted to evaluate the effectiveness of a single oral dose of ivermectin (0.15 mg/kg) in the reduction of the Mansonella ozzardi microfilaraemia and the occurrence of adverse effects in infected people compared with the control group treated with placebo. A total of 49 microfilaraemic patients were randomly selected from the municipality of Labrea, State of Amazonas, in the Brazilian Amazon. Among them, 40 patients have concluded the study, 19 treated with ivermectin and 21 treated with placebo. In the first and third days after the treatment, all the patients were clinically evaluated, and the diagnostic and quantification of blood microfilariae through blood filtration in polycarbonate membranes was performed. A significant reduction of the microfilaraemia (99.9%) was observed in the patients who received ivermectin. Slight changes in laboratory test results, without clinical importance, were seen in treated and control groups. Our results suggest that ivermectin is effective and safe for the treatment of infections caused by M. ozzardi.
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occurrence of Mansonella ozzardi diagnosed using a polycarbonate membrane in a riverside population of labrea in the western brazilian amazon
Revista Da Sociedade Brasileira De Medicina Tropical, 2016Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Luana Janaina Souza Vera, Gilberto Fontes, Gabriel De Deus Vieira, Juliana Souza De Almeida Aranha Camargo, Ricardo De Godoi Mattos Ferreira, Luis Marcelo Aranha CamargoAbstract:INTRODUCTION Mansonella ozzardi is a widely distributed filaria worm in the Amazon region. This study aimed to determine the prevalence of M. ozzardi infection in riverine communities of Labrea municipality, Amazonas State, Brazil. METHODS A diagnostic blood filtration method in a polycarbonate membrane was used. RESULTS M. ozzardi was found in 50.3% of the sample, with the highest prevalence in farmers/fishermen (69.4%; χ 2 = -19.14, p<0.001). The prevalence was higher in longer-term residents (≥11 years; 60.2%). CONCLUSIONS M. ozzardi infection rates are high near the Purus River, much greater than those previously reported based on diagnosis using thick blood smears.
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Occurrence of Mansonella ozzardi diagnosed using a polycarbonate membrane in a riverside population of Lábrea in the Western Brazilian Amazon
Sociedade Brasileira de Medicina Tropical (SBMT), 2016Co-Authors: Jansen Fernandes Medeiros, Sergio De Almeida Basano, Luana Janaina Souza Vera, Gilberto Fontes, Gabriel De Deus Vieira, Juliana Souza De Almeida Aranha Camargo, Ricardo De Godoi Mattos Ferreira, Luis Marcelo Aranha CamargoAbstract:Abstract: INTRODUCTION Mansonella ozzardi is a widely distributed filaria worm in the Amazon region. This study aimed to determine the prevalence of M. ozzardi infection in riverine communities of Lábrea municipality, Amazonas State, Brazil. METHODS A diagnostic blood filtration method in a polycarbonate membrane was used. RESULTS M. ozzardi was found in 50.3% of the sample, with the highest prevalence in farmers/fishermen (69.4%; χ 2 = -19.14, p
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sustained clearance of Mansonella ozzardi infection after treatment with ivermectin in the brazilian amazon
American Journal of Tropical Medicine and Hygiene, 2014Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Luana Janaina Souza Vera, Gilberto Fontes, Juliana Souza De Almeida Aranha Camargo, Ricardo De Godoi Mattos Ferreira, Marcos Paulo Parente Araujo, Maira Santiago Pires Parente, Pedro Di Tarique Barreto Crispim, Luis Marcelo Aranha CamargoAbstract:Therapy for mansonelliasis is challenging because there is no standard drug recommended for its treatment. This non-randomized study was conducted to evaluate the effectiveness of a single dose of 0.15 mg/kg of ivermectin to reduce Mansonella ozzardi microfilaraemia in infected persons. A total of 74 patients were studied within the municipality of Labrea, which is located in Amazonas State, Brazil. The patients were treated with ivermectin after detection of the parasite by blood examination. Significant microfilaraemia reduction was observed and its residual effect was maintained for at least 12 months. There was no significant change in the laboratory blood count, hepatic metabolites, and nitrogen-bounding compound excreta dosage values that could compromise the use of this drug, demonstrating that ivermectin has a low toxicity level.
Jose M Rubio - One of the best experts on this subject based on the ideXlab platform.
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atypical Mansonella ozzardi microfilariae from an endemic area of brazilian amazonia
American Journal of Tropical Medicine and Hygiene, 2016Co-Authors: Thuyhuong Tatang, Marta Lanza, Rogelio Lopezvelez, Sergio Luiz Bessa Luz, Francisco J Merino, Isabel Fuentes, Tatiana Amaral Pires De Almeida, Claudia Maria De Moura Abrahim, Jose M RubioAbstract:Abstract Mansonellosis is endemic in several regions of Africa, the Caribbean, and Latin America. Mansonella ozzardi and Mansonella perstans have been reported in Latin America, including the Amazon region. A morphological and molecular microfilariae study was performed in Pauini (Brazil). Blood samples were collected from 40 individuals, and were analyzed by Giemsa-stained blood film and by two different nested polymerase chain reactions which detect internal transcribed spacer-1 and the major sperm protein gene. By microscopy, 14 of 40 were positive: 11 as M. ozzardi and three as M. perstans–like infections. Both molecular methods detected 19 positive cases as M. ozzardi, including those 14 individuals detected by microscopy, without detectable genetic differences among any of the 19 positive samples. Molecular techniques showed an improvement of mansonellosis diagnosis and may become an effective tool to evaluate the present status of M. ozzardi and M. perstans in Latin America.
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a field trial of a pcr based Mansonella ozzardi diagnosis assay detects high levels of submicroscopic m ozzardi infections in both venous blood samples and fta card dried blood spots
Parasites & Vectors, 2015Co-Authors: Jansen Fernandes Medeiros, Jose M Rubio, Lucyane Bastos Tavares Silva, James Lee Crainey, Tatiana Amaral Pires De Almeida, Felipe Arley Costa PessoaAbstract:Background Mansonella ozzardi is a poorly understood human filarial parasite with a broad distribution throughout Latin America. Most of what is known about its parasitism has come from epidemiological studies that have estimated parasite incidence using light microscopy. Light microscopy can, however, miss lighter, submicroscopic, infections. In this study we have compared M. ozzardi incidence estimates made using light microscopy, with estimates made using PCR.
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nested pcr to detect and distinguish the sympatric filarial species onchocerca volvulus Mansonella ozzardi and Mansonella perstans in the amazon region
Memorias Do Instituto Oswaldo Cruz, 2010Co-Authors: Thuyhuong Ta Tang, Marta Lanza, A J Shelley, Rogelio Lopezvelez, Jose M RubioAbstract:We present filaria-nested polymerase chain reaction (PCR), which is based on amplification of first internal transcribed spacer rDNA to distinguish three parasitic filarial species (Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans) that can be found in the Amazon Region. Nested PCR-based identifications yielded the same results as those utilizing morphological characters. Nested PCR is highly sensitive and specific and it detects low-level infections in both humans and vectors. No cross-amplifications were observed with various other blood parasites and no false-positive results were obtained with the nested PCR. The method works efficiently with whole-blood, blood-spot and skin biopsy samples. Our method may thus be suitable for assessing the efficacy of filaria control programmes in Amazonia by recording parasite infections in both the human host and the vector. By specifically differentiating the major sympatric species of filaria, this technique could also enhance epidemiological research in the region.