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Jansen Fernandes Medeiros - One of the best experts on this subject based on the ideXlab platform.
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sensitivity of diagnostic methods for mansonella ozzardi microfilariae detection in the brazilian amazon region
Memorias Do Instituto Oswaldo Cruz, 2018Co-Authors: Jansen Fernandes Medeiros, Felipe Arley Costa Pessoa, Gilberto Fontes, Jacob Moyses Cohen, Moreno S Rodrigues, Edmar Vaz De Andrade, Vilma Lopes Do Nascimento, Marilaine MartinsAbstract:BACKGROUND The human filarial worm Mansonella ozzardi is highly endemic in the large tributaries of the Amazon River. This infection is still highly neglected and can be falsely negative when microfilariae levels are low. OBJECTIVES This study investigated the frequency of individuals with M. ozzardi in riverine communities in Coari municipality, Brazilian Amazon. METHODS Different diagnostic methods including polymerase chain reaction (PCR), blood polycarbonate membrane filtration (PCMF), Knott's method (Knott), digital thick blood smears (DTBS) and venous thick blood smears (VTBS) were used to compare sensitivity and specificity among the methods. Data were analysed using PCMF and Bayesian latent class models (BLCM) as the gold standard. We used BLCM to calculate the prevalence of Mansonelliasis based on the results of five diagnostic methods. FINDINGS The prevalence of Mansonelliasis was 35.4% by PCMF and 30.1% by BLCM. PCR and Knott methods both possessed high sensitivity. Sensitivity relative to PCMF was 98.5% [95% confidence interval (CI): 92.0 - 99.7] for PCR and 83.5% (95% CI: 72.9 - 90.5) for Knott. Sensitivity derived by BLCM was 100% (95% CI 93.7 - 100) for PCMF, 100% (95% CI: 93.7 - 100) for PCR and 98.3% (95% CI: 90.6 - 99.9) for Knott. The odds ratio of being diagnosed as microfilaremic increased with age but did not differ between genders. Microfilariae loads were higher in subjects aged 30 - 45 and 45 - 60 years. MAIN CONCLUSIONS PCMF and PCR were the best methods to assess the prevalence of Mansonelliasis in our samples. As such, using these methods could lead to higher prevalence of Mansonelliasis in this region than the most commonly used method (i.e., thick blood smears).
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phase iii clinical trial to evaluate ivermectin in the reduction of mansonella ozzardi infection in the brazilian amazon
American Journal of Tropical Medicine and Hygiene, 2018Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Gilberto Fontes, Ricardo De Godoi Mattos Ferreira, Juliana De Souza Almeida Aranha Camargo, Antonieta Relvas Pereira, Mayara Costa De Oliveira Laudisse, Luis Marcelo Aranha CamargoAbstract:The treatment of Mansonelliasis is still a challenge because there are few clinical trials for the treatment of the disease. This double-blind, randomized, placebo-controlled study (phase III clinical trial) was conducted to evaluate the effectiveness of a single oral dose of ivermectin (0.15 mg/kg) in the reduction of the Mansonella ozzardi microfilaraemia and the occurrence of adverse effects in infected people compared with the control group treated with placebo. A total of 49 microfilaraemic patients were randomly selected from the municipality of Labrea, State of Amazonas, in the Brazilian Amazon. Among them, 40 patients have concluded the study, 19 treated with ivermectin and 21 treated with placebo. In the first and third days after the treatment, all the patients were clinically evaluated, and the diagnostic and quantification of blood microfilariae through blood filtration in polycarbonate membranes was performed. A significant reduction of the microfilaraemia (99.9%) was observed in the patients who received ivermectin. Slight changes in laboratory test results, without clinical importance, were seen in treated and control groups. Our results suggest that ivermectin is effective and safe for the treatment of infections caused by M. ozzardi.
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epidemiological snapshot of the Mansonelliasis infection in the amazonian riverine communities in two contiguous municipalities of solimoes river amazonas state brazil
Revista Pan-Amazônica de Saúde, 2015Co-Authors: Jansen Fernandes Medeiros, Felipe Arley Costa Pessoa, Moreno S Rodrigues, Marilaine MartinsAbstract:This study was conducted to gather updated information on the occurrence of Mansonella ozzardi in Codajas, and report new information about filaria found in the riverine communities of Coari, Amazonas State, Brazil. The prevalence (thick blood smear) of infection was 9.4% and 22.0% in Codajas and Coari, respectively. Men (17.1%) had a higher prevalence than women (11.2%) (ANOVA χ2 = -4.93; p = 0.02); and the rates significantly increased with age (ANOVA χ2 = -11.51; p < 0.001) and in certain occupations such as farming/fishing (29.2%) and retired (27.6%). Municipalities located in the Solimoes river have attracted large numbers of migrant workers due to oil and gas exploration in the area, and this may facilitate the spread of filariasis to other non-endemic areas.
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sustained clearance of mansonella ozzardi infection after treatment with ivermectin in the brazilian amazon
American Journal of Tropical Medicine and Hygiene, 2014Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Luana Janaina Souza Vera, Gilberto Fontes, Juliana Souza De Almeida Aranha Camargo, Ricardo De Godoi Mattos Ferreira, Marcos Paulo Parente Araujo, Maira Santiago Pires Parente, Pedro Di Tarique Barreto Crispim, Luis Marcelo Aranha CamargoAbstract:Therapy for Mansonelliasis is challenging because there is no standard drug recommended for its treatment. This non-randomized study was conducted to evaluate the effectiveness of a single dose of 0.15 mg/kg of ivermectin to reduce Mansonella ozzardi microfilaraemia in infected persons. A total of 74 patients were studied within the municipality of Labrea, which is located in Amazonas State, Brazil. The patients were treated with ivermectin after detection of the parasite by blood examination. Significant microfilaraemia reduction was observed and its residual effect was maintained for at least 12 months. There was no significant change in the laboratory blood count, hepatic metabolites, and nitrogen-bounding compound excreta dosage values that could compromise the use of this drug, demonstrating that ivermectin has a low toxicity level.
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mansonella ozzardi in the municipality of tefe amazonas brazil 60 years after the first report an epidemiologic study
Memorias Do Instituto Oswaldo Cruz, 2014Co-Authors: Jansen Fernandes Medeiros, Cristovao Alves Da Costa, Moreno S Rodrigues, Tony Hiroshi Katsuragawa, Felipe Arley Costa PessoaAbstract:The prevalence of Mansonelliasis was studied in the municipality of Tefe, state of Amazonas, Brazil. The prevalence (thick blood smear method) was 13.6% (147/1,078), higher in the Solimoes River region (16.3%) than in the Tefe River region (6.3%). In the sampled communities in the Solimoes River region, a higher density of cases was observed, as indicated by a kernel analysis (odds ratio 0.34; 95% confidence interval: 0.20-0.57). Males had a higher prevalence (χ2 = 31.292, p < 0.001) than women. Mansonella ozzardi prevalence was higher in retirees and farmers (28.9% and 27%, respectively). Prevalence also significantly increased with age (χ2 = -128.17, p < 0.001), with the highest numbers occurring in persons older than 67 years.
Luis Marcelo Aranha Camargo - One of the best experts on this subject based on the ideXlab platform.
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phase iii clinical trial to evaluate ivermectin in the reduction of mansonella ozzardi infection in the brazilian amazon
American Journal of Tropical Medicine and Hygiene, 2018Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Gilberto Fontes, Ricardo De Godoi Mattos Ferreira, Juliana De Souza Almeida Aranha Camargo, Antonieta Relvas Pereira, Mayara Costa De Oliveira Laudisse, Luis Marcelo Aranha CamargoAbstract:The treatment of Mansonelliasis is still a challenge because there are few clinical trials for the treatment of the disease. This double-blind, randomized, placebo-controlled study (phase III clinical trial) was conducted to evaluate the effectiveness of a single oral dose of ivermectin (0.15 mg/kg) in the reduction of the Mansonella ozzardi microfilaraemia and the occurrence of adverse effects in infected people compared with the control group treated with placebo. A total of 49 microfilaraemic patients were randomly selected from the municipality of Labrea, State of Amazonas, in the Brazilian Amazon. Among them, 40 patients have concluded the study, 19 treated with ivermectin and 21 treated with placebo. In the first and third days after the treatment, all the patients were clinically evaluated, and the diagnostic and quantification of blood microfilariae through blood filtration in polycarbonate membranes was performed. A significant reduction of the microfilaraemia (99.9%) was observed in the patients who received ivermectin. Slight changes in laboratory test results, without clinical importance, were seen in treated and control groups. Our results suggest that ivermectin is effective and safe for the treatment of infections caused by M. ozzardi.
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sustained clearance of mansonella ozzardi infection after treatment with ivermectin in the brazilian amazon
American Journal of Tropical Medicine and Hygiene, 2014Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Luana Janaina Souza Vera, Gilberto Fontes, Juliana Souza De Almeida Aranha Camargo, Ricardo De Godoi Mattos Ferreira, Marcos Paulo Parente Araujo, Maira Santiago Pires Parente, Pedro Di Tarique Barreto Crispim, Luis Marcelo Aranha CamargoAbstract:Therapy for Mansonelliasis is challenging because there is no standard drug recommended for its treatment. This non-randomized study was conducted to evaluate the effectiveness of a single dose of 0.15 mg/kg of ivermectin to reduce Mansonella ozzardi microfilaraemia in infected persons. A total of 74 patients were studied within the municipality of Labrea, which is located in Amazonas State, Brazil. The patients were treated with ivermectin after detection of the parasite by blood examination. Significant microfilaraemia reduction was observed and its residual effect was maintained for at least 12 months. There was no significant change in the laboratory blood count, hepatic metabolites, and nitrogen-bounding compound excreta dosage values that could compromise the use of this drug, demonstrating that ivermectin has a low toxicity level.
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Mansonelliasis: A Brazilian Neglected Disease
Revista de Patologia Tropical, 2014Co-Authors: Jansen Fernandes Medeiros, Felipe Arley Costa Pessoa, Luis Marcelo Aranha CamargoAbstract:Mansonelliasis is a filariasis whose etiological agents are Mansonella ozzardi, Mansonella perstans and Mansonella streptocerca. Only the first two cited species occur in Brazil. M. ozzardi is widely distributed in Amazonas state and it is found along the rivers Solimoes, Purus, Negro and their tributaries while M. perstans is restricted to the Upper Rio Negro. In this update, we report the occurrence of M. ozzardi in Amazonas since the 1950s, and we show that over the years this filariasis has been sustained with high prevalence, while maintaining a constant cycle of transmission in endemic areas due to the lack of treatment and control policies. M. perstans has so far only been recorded in indigenous populations in the Upper Rio Negro. However, the continuous flow of migrants to other regions may cause an expansion of this infection.
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current title Mansonelliasis in rondonia brazil investigation in the occurrence of mansonella ozzardi in the state of rondonia western amazonia brazil mansonelose em rondonia brasil investigacao da ocorrencia da mansonella ozzardi no estado de ro
Revista Da Sociedade Brasileira De Medicina Tropical, 2012Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Juliana Aranha Camargo, Luana Janaina Souza Vera, Suzane Neves Velasques, Guilherme Maerschner Ogawa, Gilberto Fontes, Luis Marcelo Aranha CamargoAbstract:Abstract Introduction: Mansonella ozzardi is a filaria, transmitted by Diptera Simuliidae flies (black fies), is widely spread in the Amazon, while the occurrence of Mansonelliasis in the state of Rondonia has not been reported so far. The existence of such a p arasitic disease in the state was investigated. Methodology: 4,452 people were examined alongside the Rivers named Madeira, Mamore, Guapore, Machado, and Preto, in the state of Rondonia by means of the thick blood smears method. Alongside the rivers named Machado and Guapore 2,112 adult simuliids specimens were captured. Results: There has not been found infected people, although there has been found simuliids with vectorial potential as Cerqueirellum pydanielli, C. argentiscutum and C. amazonicum. Conclusion: Despite of the existence of flies with vectorial potential, and the proximity to areas of high prevalence of Mansonelliasis in the Brazilian western Amazon territory, there has not been found people infected by this filariasis in the State of Rondonia. Keywords: Mansonella ozzardi – microfilariae – Simuliidae - Rondonia RESUMO Introducao: Mansonella ozzardi e uma filaria que tem como vetor dipteros simulideos e e amplamente distribuida na Amazonia. Nao ha informacoes sobre a ocorrencia da mansonelose em Rondonia. Procurou-se verificar a existencia da parasitose no estado. Metodologia: Foram examinadas 4.452 pessoas as margens dos rios Madeira, Mamore, Guapore, Machado e Preto em Rondonia, atraves da tecnica da gota espessa de sangue. Capturou-se as margens do rio Machado e Guapore 2.112 simulideos adultos das especies Cerqueirellum pydanieli e Chirostilbia pertinax. Resultados: Nao foram encontradas pessoas infectadas e foram encontrados simulideos com potencial de transmissao da parasitose. Conclusao: Apesar da existencia de vetores com potencial de transmissao e da proximidade com areas de alta prevalencia de mansonelose na Amazonia ocidental do Brasil, nao foram encontrados portadores desta filariose em Rondonia. Palavras-chaves: Mansonella ozzardi – microfilarias – Simuliidae - Rondonia
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investigation of the occurrence of mansonella ozzardi in the state of rondonia western amazonia brazil
Revista Da Sociedade Brasileira De Medicina Tropical, 2011Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Luana Janaina Souza Vera, Suzane Neves Velasques, Guilherme Maerschner Ogawa, Gilberto Fontes, Juliana De Souza Almeida Aranha Camargo, Luis Marcelo Aranha CamargoAbstract:INTRODUCTION: Mansonella ozzardi, a human filarial nematode transmitted by Diptera Simuliidae flies (black flies), is widely spread in the Amazon, while the occurrence of Mansonelliasis in the State of Rondonia (Western Amazonia) has not been reported thus far. The existence of this parasitic disease in this state is investigated in this study. METHODS: A total of 4,452 people who lived alongside the Madeira, Mamore, Guapore, Machado and Preto Rivers, in the State of Rondonia, were examined using the thick blood smear method. Alongside the Machado and Guapore rivers, 2,112 adult simuliid specimens were captured. RESULTS: Infected people were not reported, although simuliids with vector potential such as Cerqueirellum pydanielli, C. argentiscutum and C. amazonicum were found. CONCLUSIONS: This study registered no infected people. This finding may be explained by the individuals' low level of microfilaremia that is below the limit of detection of the diagnostic method used in the study or insufficient migration of people infected with M. ozzardi from endemic areas, making it impossible to introduce the disease into Rondonia. Further studies using more sensitive diagnostic techniques could shed light on this question.
Felipe Arley Costa Pessoa - One of the best experts on this subject based on the ideXlab platform.
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sensitivity of diagnostic methods for mansonella ozzardi microfilariae detection in the brazilian amazon region
Memorias Do Instituto Oswaldo Cruz, 2018Co-Authors: Jansen Fernandes Medeiros, Felipe Arley Costa Pessoa, Gilberto Fontes, Jacob Moyses Cohen, Moreno S Rodrigues, Edmar Vaz De Andrade, Vilma Lopes Do Nascimento, Marilaine MartinsAbstract:BACKGROUND The human filarial worm Mansonella ozzardi is highly endemic in the large tributaries of the Amazon River. This infection is still highly neglected and can be falsely negative when microfilariae levels are low. OBJECTIVES This study investigated the frequency of individuals with M. ozzardi in riverine communities in Coari municipality, Brazilian Amazon. METHODS Different diagnostic methods including polymerase chain reaction (PCR), blood polycarbonate membrane filtration (PCMF), Knott's method (Knott), digital thick blood smears (DTBS) and venous thick blood smears (VTBS) were used to compare sensitivity and specificity among the methods. Data were analysed using PCMF and Bayesian latent class models (BLCM) as the gold standard. We used BLCM to calculate the prevalence of Mansonelliasis based on the results of five diagnostic methods. FINDINGS The prevalence of Mansonelliasis was 35.4% by PCMF and 30.1% by BLCM. PCR and Knott methods both possessed high sensitivity. Sensitivity relative to PCMF was 98.5% [95% confidence interval (CI): 92.0 - 99.7] for PCR and 83.5% (95% CI: 72.9 - 90.5) for Knott. Sensitivity derived by BLCM was 100% (95% CI 93.7 - 100) for PCMF, 100% (95% CI: 93.7 - 100) for PCR and 98.3% (95% CI: 90.6 - 99.9) for Knott. The odds ratio of being diagnosed as microfilaremic increased with age but did not differ between genders. Microfilariae loads were higher in subjects aged 30 - 45 and 45 - 60 years. MAIN CONCLUSIONS PCMF and PCR were the best methods to assess the prevalence of Mansonelliasis in our samples. As such, using these methods could lead to higher prevalence of Mansonelliasis in this region than the most commonly used method (i.e., thick blood smears).
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epidemiological snapshot of the Mansonelliasis infection in the amazonian riverine communities in two contiguous municipalities of solimoes river amazonas state brazil
Revista Pan-Amazônica de Saúde, 2015Co-Authors: Jansen Fernandes Medeiros, Felipe Arley Costa Pessoa, Moreno S Rodrigues, Marilaine MartinsAbstract:This study was conducted to gather updated information on the occurrence of Mansonella ozzardi in Codajas, and report new information about filaria found in the riverine communities of Coari, Amazonas State, Brazil. The prevalence (thick blood smear) of infection was 9.4% and 22.0% in Codajas and Coari, respectively. Men (17.1%) had a higher prevalence than women (11.2%) (ANOVA χ2 = -4.93; p = 0.02); and the rates significantly increased with age (ANOVA χ2 = -11.51; p < 0.001) and in certain occupations such as farming/fishing (29.2%) and retired (27.6%). Municipalities located in the Solimoes river have attracted large numbers of migrant workers due to oil and gas exploration in the area, and this may facilitate the spread of filariasis to other non-endemic areas.
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mansonella ozzardi in the municipality of tefe amazonas brazil 60 years after the first report an epidemiologic study
Memorias Do Instituto Oswaldo Cruz, 2014Co-Authors: Jansen Fernandes Medeiros, Cristovao Alves Da Costa, Moreno S Rodrigues, Tony Hiroshi Katsuragawa, Felipe Arley Costa PessoaAbstract:The prevalence of Mansonelliasis was studied in the municipality of Tefe, state of Amazonas, Brazil. The prevalence (thick blood smear method) was 13.6% (147/1,078), higher in the Solimoes River region (16.3%) than in the Tefe River region (6.3%). In the sampled communities in the Solimoes River region, a higher density of cases was observed, as indicated by a kernel analysis (odds ratio 0.34; 95% confidence interval: 0.20-0.57). Males had a higher prevalence (χ2 = 31.292, p < 0.001) than women. Mansonella ozzardi prevalence was higher in retirees and farmers (28.9% and 27%, respectively). Prevalence also significantly increased with age (χ2 = -128.17, p < 0.001), with the highest numbers occurring in persons older than 67 years.
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Mansonelliasis: A Brazilian Neglected Disease
Revista de Patologia Tropical, 2014Co-Authors: Jansen Fernandes Medeiros, Felipe Arley Costa Pessoa, Luis Marcelo Aranha CamargoAbstract:Mansonelliasis is a filariasis whose etiological agents are Mansonella ozzardi, Mansonella perstans and Mansonella streptocerca. Only the first two cited species occur in Brazil. M. ozzardi is widely distributed in Amazonas state and it is found along the rivers Solimoes, Purus, Negro and their tributaries while M. perstans is restricted to the Upper Rio Negro. In this update, we report the occurrence of M. ozzardi in Amazonas since the 1950s, and we show that over the years this filariasis has been sustained with high prevalence, while maintaining a constant cycle of transmission in endemic areas due to the lack of treatment and control policies. M. perstans has so far only been recorded in indigenous populations in the Upper Rio Negro. However, the continuous flow of migrants to other regions may cause an expansion of this infection.
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mansonella ozzardi nematoda onchocercidae in the riverine population of the tefe river state of amazonia brazil
Revista Da Sociedade Brasileira De Medicina Tropical, 2014Co-Authors: Jansen Fernandes Medeiros, Cristovao Alves Da Costa, Alita Moura De Lima, Felipe Arley Costa PessoaAbstract:Introduction: This study assessed the prevalence of Mansonella ozzardi in riverine communities of the Tefe River, Amazonas, Brazil. Methods: The prevalence of M. ozzardi was estimated by microscopic examination of thick blood smears. Results: The M. ozzardi prevalence rate was 6.3% (19/300). Filarial infection was found in 8 of the 11 communities surveyed, with prevalence rates varying from 2.5% to 22.2%. Conclusions: Tefe is a region of oil and natural gas exploration, in which there is a high turnover of workers. Migration patterns may facilitate the dissemination of Mansonelliasis to other regions.
Sergio De Almeida Basano - One of the best experts on this subject based on the ideXlab platform.
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phase iii clinical trial to evaluate ivermectin in the reduction of mansonella ozzardi infection in the brazilian amazon
American Journal of Tropical Medicine and Hygiene, 2018Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Gilberto Fontes, Ricardo De Godoi Mattos Ferreira, Juliana De Souza Almeida Aranha Camargo, Antonieta Relvas Pereira, Mayara Costa De Oliveira Laudisse, Luis Marcelo Aranha CamargoAbstract:The treatment of Mansonelliasis is still a challenge because there are few clinical trials for the treatment of the disease. This double-blind, randomized, placebo-controlled study (phase III clinical trial) was conducted to evaluate the effectiveness of a single oral dose of ivermectin (0.15 mg/kg) in the reduction of the Mansonella ozzardi microfilaraemia and the occurrence of adverse effects in infected people compared with the control group treated with placebo. A total of 49 microfilaraemic patients were randomly selected from the municipality of Labrea, State of Amazonas, in the Brazilian Amazon. Among them, 40 patients have concluded the study, 19 treated with ivermectin and 21 treated with placebo. In the first and third days after the treatment, all the patients were clinically evaluated, and the diagnostic and quantification of blood microfilariae through blood filtration in polycarbonate membranes was performed. A significant reduction of the microfilaraemia (99.9%) was observed in the patients who received ivermectin. Slight changes in laboratory test results, without clinical importance, were seen in treated and control groups. Our results suggest that ivermectin is effective and safe for the treatment of infections caused by M. ozzardi.
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sustained clearance of mansonella ozzardi infection after treatment with ivermectin in the brazilian amazon
American Journal of Tropical Medicine and Hygiene, 2014Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Luana Janaina Souza Vera, Gilberto Fontes, Juliana Souza De Almeida Aranha Camargo, Ricardo De Godoi Mattos Ferreira, Marcos Paulo Parente Araujo, Maira Santiago Pires Parente, Pedro Di Tarique Barreto Crispim, Luis Marcelo Aranha CamargoAbstract:Therapy for Mansonelliasis is challenging because there is no standard drug recommended for its treatment. This non-randomized study was conducted to evaluate the effectiveness of a single dose of 0.15 mg/kg of ivermectin to reduce Mansonella ozzardi microfilaraemia in infected persons. A total of 74 patients were studied within the municipality of Labrea, which is located in Amazonas State, Brazil. The patients were treated with ivermectin after detection of the parasite by blood examination. Significant microfilaraemia reduction was observed and its residual effect was maintained for at least 12 months. There was no significant change in the laboratory blood count, hepatic metabolites, and nitrogen-bounding compound excreta dosage values that could compromise the use of this drug, demonstrating that ivermectin has a low toxicity level.
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current title Mansonelliasis in rondonia brazil investigation in the occurrence of mansonella ozzardi in the state of rondonia western amazonia brazil mansonelose em rondonia brasil investigacao da ocorrencia da mansonella ozzardi no estado de ro
Revista Da Sociedade Brasileira De Medicina Tropical, 2012Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Juliana Aranha Camargo, Luana Janaina Souza Vera, Suzane Neves Velasques, Guilherme Maerschner Ogawa, Gilberto Fontes, Luis Marcelo Aranha CamargoAbstract:Abstract Introduction: Mansonella ozzardi is a filaria, transmitted by Diptera Simuliidae flies (black fies), is widely spread in the Amazon, while the occurrence of Mansonelliasis in the state of Rondonia has not been reported so far. The existence of such a p arasitic disease in the state was investigated. Methodology: 4,452 people were examined alongside the Rivers named Madeira, Mamore, Guapore, Machado, and Preto, in the state of Rondonia by means of the thick blood smears method. Alongside the rivers named Machado and Guapore 2,112 adult simuliids specimens were captured. Results: There has not been found infected people, although there has been found simuliids with vectorial potential as Cerqueirellum pydanielli, C. argentiscutum and C. amazonicum. Conclusion: Despite of the existence of flies with vectorial potential, and the proximity to areas of high prevalence of Mansonelliasis in the Brazilian western Amazon territory, there has not been found people infected by this filariasis in the State of Rondonia. Keywords: Mansonella ozzardi – microfilariae – Simuliidae - Rondonia RESUMO Introducao: Mansonella ozzardi e uma filaria que tem como vetor dipteros simulideos e e amplamente distribuida na Amazonia. Nao ha informacoes sobre a ocorrencia da mansonelose em Rondonia. Procurou-se verificar a existencia da parasitose no estado. Metodologia: Foram examinadas 4.452 pessoas as margens dos rios Madeira, Mamore, Guapore, Machado e Preto em Rondonia, atraves da tecnica da gota espessa de sangue. Capturou-se as margens do rio Machado e Guapore 2.112 simulideos adultos das especies Cerqueirellum pydanieli e Chirostilbia pertinax. Resultados: Nao foram encontradas pessoas infectadas e foram encontrados simulideos com potencial de transmissao da parasitose. Conclusao: Apesar da existencia de vetores com potencial de transmissao e da proximidade com areas de alta prevalencia de mansonelose na Amazonia ocidental do Brasil, nao foram encontrados portadores desta filariose em Rondonia. Palavras-chaves: Mansonella ozzardi – microfilarias – Simuliidae - Rondonia
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investigation of the occurrence of mansonella ozzardi in the state of rondonia western amazonia brazil
Revista Da Sociedade Brasileira De Medicina Tropical, 2011Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Luana Janaina Souza Vera, Suzane Neves Velasques, Guilherme Maerschner Ogawa, Gilberto Fontes, Juliana De Souza Almeida Aranha Camargo, Luis Marcelo Aranha CamargoAbstract:INTRODUCTION: Mansonella ozzardi, a human filarial nematode transmitted by Diptera Simuliidae flies (black flies), is widely spread in the Amazon, while the occurrence of Mansonelliasis in the State of Rondonia (Western Amazonia) has not been reported thus far. The existence of this parasitic disease in this state is investigated in this study. METHODS: A total of 4,452 people who lived alongside the Madeira, Mamore, Guapore, Machado and Preto Rivers, in the State of Rondonia, were examined using the thick blood smear method. Alongside the Machado and Guapore rivers, 2,112 adult simuliid specimens were captured. RESULTS: Infected people were not reported, although simuliids with vector potential such as Cerqueirellum pydanielli, C. argentiscutum and C. amazonicum were found. CONCLUSIONS: This study registered no infected people. This finding may be explained by the individuals' low level of microfilaremia that is below the limit of detection of the diagnostic method used in the study or insufficient migration of people infected with M. ozzardi from endemic areas, making it impossible to introduce the disease into Rondonia. Further studies using more sensitive diagnostic techniques could shed light on this question.
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improvement of a pcr test to diagnose infection by mansonella ozzardi
Revista Da Sociedade Brasileira De Medicina Tropical, 2011Co-Authors: Luana Janaina Souza Vera, Jansen Fernandes Medeiros, Sergio De Almeida Basano, Gilberto Fontes, Ricardo De Godoi Mattos Ferreira, Juliana De Souza Almeida Aranha Camargo, Andonai Krauze De Franca, Almeida Andrade Casseb, Luis Marcelo Aranha CamargoAbstract:INTRODUCTION: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. The common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia. METHODS: In order to increase diagnosis efficacy, the PCR technique was improved. RESULTS AND CONCLUSIONS: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood filtration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears.
Gilberto Fontes - One of the best experts on this subject based on the ideXlab platform.
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sensitivity of diagnostic methods for mansonella ozzardi microfilariae detection in the brazilian amazon region
Memorias Do Instituto Oswaldo Cruz, 2018Co-Authors: Jansen Fernandes Medeiros, Felipe Arley Costa Pessoa, Gilberto Fontes, Jacob Moyses Cohen, Moreno S Rodrigues, Edmar Vaz De Andrade, Vilma Lopes Do Nascimento, Marilaine MartinsAbstract:BACKGROUND The human filarial worm Mansonella ozzardi is highly endemic in the large tributaries of the Amazon River. This infection is still highly neglected and can be falsely negative when microfilariae levels are low. OBJECTIVES This study investigated the frequency of individuals with M. ozzardi in riverine communities in Coari municipality, Brazilian Amazon. METHODS Different diagnostic methods including polymerase chain reaction (PCR), blood polycarbonate membrane filtration (PCMF), Knott's method (Knott), digital thick blood smears (DTBS) and venous thick blood smears (VTBS) were used to compare sensitivity and specificity among the methods. Data were analysed using PCMF and Bayesian latent class models (BLCM) as the gold standard. We used BLCM to calculate the prevalence of Mansonelliasis based on the results of five diagnostic methods. FINDINGS The prevalence of Mansonelliasis was 35.4% by PCMF and 30.1% by BLCM. PCR and Knott methods both possessed high sensitivity. Sensitivity relative to PCMF was 98.5% [95% confidence interval (CI): 92.0 - 99.7] for PCR and 83.5% (95% CI: 72.9 - 90.5) for Knott. Sensitivity derived by BLCM was 100% (95% CI 93.7 - 100) for PCMF, 100% (95% CI: 93.7 - 100) for PCR and 98.3% (95% CI: 90.6 - 99.9) for Knott. The odds ratio of being diagnosed as microfilaremic increased with age but did not differ between genders. Microfilariae loads were higher in subjects aged 30 - 45 and 45 - 60 years. MAIN CONCLUSIONS PCMF and PCR were the best methods to assess the prevalence of Mansonelliasis in our samples. As such, using these methods could lead to higher prevalence of Mansonelliasis in this region than the most commonly used method (i.e., thick blood smears).
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phase iii clinical trial to evaluate ivermectin in the reduction of mansonella ozzardi infection in the brazilian amazon
American Journal of Tropical Medicine and Hygiene, 2018Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Gilberto Fontes, Ricardo De Godoi Mattos Ferreira, Juliana De Souza Almeida Aranha Camargo, Antonieta Relvas Pereira, Mayara Costa De Oliveira Laudisse, Luis Marcelo Aranha CamargoAbstract:The treatment of Mansonelliasis is still a challenge because there are few clinical trials for the treatment of the disease. This double-blind, randomized, placebo-controlled study (phase III clinical trial) was conducted to evaluate the effectiveness of a single oral dose of ivermectin (0.15 mg/kg) in the reduction of the Mansonella ozzardi microfilaraemia and the occurrence of adverse effects in infected people compared with the control group treated with placebo. A total of 49 microfilaraemic patients were randomly selected from the municipality of Labrea, State of Amazonas, in the Brazilian Amazon. Among them, 40 patients have concluded the study, 19 treated with ivermectin and 21 treated with placebo. In the first and third days after the treatment, all the patients were clinically evaluated, and the diagnostic and quantification of blood microfilariae through blood filtration in polycarbonate membranes was performed. A significant reduction of the microfilaraemia (99.9%) was observed in the patients who received ivermectin. Slight changes in laboratory test results, without clinical importance, were seen in treated and control groups. Our results suggest that ivermectin is effective and safe for the treatment of infections caused by M. ozzardi.
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sustained clearance of mansonella ozzardi infection after treatment with ivermectin in the brazilian amazon
American Journal of Tropical Medicine and Hygiene, 2014Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Luana Janaina Souza Vera, Gilberto Fontes, Juliana Souza De Almeida Aranha Camargo, Ricardo De Godoi Mattos Ferreira, Marcos Paulo Parente Araujo, Maira Santiago Pires Parente, Pedro Di Tarique Barreto Crispim, Luis Marcelo Aranha CamargoAbstract:Therapy for Mansonelliasis is challenging because there is no standard drug recommended for its treatment. This non-randomized study was conducted to evaluate the effectiveness of a single dose of 0.15 mg/kg of ivermectin to reduce Mansonella ozzardi microfilaraemia in infected persons. A total of 74 patients were studied within the municipality of Labrea, which is located in Amazonas State, Brazil. The patients were treated with ivermectin after detection of the parasite by blood examination. Significant microfilaraemia reduction was observed and its residual effect was maintained for at least 12 months. There was no significant change in the laboratory blood count, hepatic metabolites, and nitrogen-bounding compound excreta dosage values that could compromise the use of this drug, demonstrating that ivermectin has a low toxicity level.
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current title Mansonelliasis in rondonia brazil investigation in the occurrence of mansonella ozzardi in the state of rondonia western amazonia brazil mansonelose em rondonia brasil investigacao da ocorrencia da mansonella ozzardi no estado de ro
Revista Da Sociedade Brasileira De Medicina Tropical, 2012Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Juliana Aranha Camargo, Luana Janaina Souza Vera, Suzane Neves Velasques, Guilherme Maerschner Ogawa, Gilberto Fontes, Luis Marcelo Aranha CamargoAbstract:Abstract Introduction: Mansonella ozzardi is a filaria, transmitted by Diptera Simuliidae flies (black fies), is widely spread in the Amazon, while the occurrence of Mansonelliasis in the state of Rondonia has not been reported so far. The existence of such a p arasitic disease in the state was investigated. Methodology: 4,452 people were examined alongside the Rivers named Madeira, Mamore, Guapore, Machado, and Preto, in the state of Rondonia by means of the thick blood smears method. Alongside the rivers named Machado and Guapore 2,112 adult simuliids specimens were captured. Results: There has not been found infected people, although there has been found simuliids with vectorial potential as Cerqueirellum pydanielli, C. argentiscutum and C. amazonicum. Conclusion: Despite of the existence of flies with vectorial potential, and the proximity to areas of high prevalence of Mansonelliasis in the Brazilian western Amazon territory, there has not been found people infected by this filariasis in the State of Rondonia. Keywords: Mansonella ozzardi – microfilariae – Simuliidae - Rondonia RESUMO Introducao: Mansonella ozzardi e uma filaria que tem como vetor dipteros simulideos e e amplamente distribuida na Amazonia. Nao ha informacoes sobre a ocorrencia da mansonelose em Rondonia. Procurou-se verificar a existencia da parasitose no estado. Metodologia: Foram examinadas 4.452 pessoas as margens dos rios Madeira, Mamore, Guapore, Machado e Preto em Rondonia, atraves da tecnica da gota espessa de sangue. Capturou-se as margens do rio Machado e Guapore 2.112 simulideos adultos das especies Cerqueirellum pydanieli e Chirostilbia pertinax. Resultados: Nao foram encontradas pessoas infectadas e foram encontrados simulideos com potencial de transmissao da parasitose. Conclusao: Apesar da existencia de vetores com potencial de transmissao e da proximidade com areas de alta prevalencia de mansonelose na Amazonia ocidental do Brasil, nao foram encontrados portadores desta filariose em Rondonia. Palavras-chaves: Mansonella ozzardi – microfilarias – Simuliidae - Rondonia
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investigation of the occurrence of mansonella ozzardi in the state of rondonia western amazonia brazil
Revista Da Sociedade Brasileira De Medicina Tropical, 2011Co-Authors: Sergio De Almeida Basano, Jansen Fernandes Medeiros, Luana Janaina Souza Vera, Suzane Neves Velasques, Guilherme Maerschner Ogawa, Gilberto Fontes, Juliana De Souza Almeida Aranha Camargo, Luis Marcelo Aranha CamargoAbstract:INTRODUCTION: Mansonella ozzardi, a human filarial nematode transmitted by Diptera Simuliidae flies (black flies), is widely spread in the Amazon, while the occurrence of Mansonelliasis in the State of Rondonia (Western Amazonia) has not been reported thus far. The existence of this parasitic disease in this state is investigated in this study. METHODS: A total of 4,452 people who lived alongside the Madeira, Mamore, Guapore, Machado and Preto Rivers, in the State of Rondonia, were examined using the thick blood smear method. Alongside the Machado and Guapore rivers, 2,112 adult simuliid specimens were captured. RESULTS: Infected people were not reported, although simuliids with vector potential such as Cerqueirellum pydanielli, C. argentiscutum and C. amazonicum were found. CONCLUSIONS: This study registered no infected people. This finding may be explained by the individuals' low level of microfilaremia that is below the limit of detection of the diagnostic method used in the study or insufficient migration of people infected with M. ozzardi from endemic areas, making it impossible to introduce the disease into Rondonia. Further studies using more sensitive diagnostic techniques could shed light on this question.