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Si Zhang - One of the best experts on this subject based on the ideXlab platform.

  • sinomicrobium oceani gen nov sp nov a member of the family flavobacteriaceae isolated from Marine Sediment
    International Journal of Systematic and Evolutionary Microbiology, 2013
    Co-Authors: Xin-peng Tian, Wen-jun Li, Ying Xu, Jie Li, Si Zhang
    Abstract:

    A Marine bacterium, designated SCSIO 03483(T), was isolated from a Marine Sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-coloured pigment on nutrient agar medium or Marine agar 2216. Optimal growth occurred in the presence of 0-4 % (w/v) NaCI, at pH 7.0 and a temperature range of 28-37 degrees C. 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarity with Imtechella halotolerans K1(T) (92.7%). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the isolate shared a lineage with members of the genera Imtechella, Joostella and Zhouia. Phospholipids were phosphatidylethanolamine, two unidentified aminolipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C-15:0, iso-C-17:0 3-OH and summed feature 3 (C-16:1 omega 6c/C-16:1 omega 7c). The DNA G+C content of strain SCSIO 03483(T) was 38.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483(T) represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Sinomicrobium oceani gen. nov., sp. nov. is proposed. The type strain of Sinobacterium oceani is SCSIO 03483(T) (=KCTC 23994(T=)CGMCC 1.12145(T)).

  • Sinomicrobium oceani gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from Marine Sediment.
    International journal of systematic and evolutionary microbiology, 2012
    Co-Authors: Ying Xu, Xin-peng Tian, Wen-jun Li, Jie Li, Si Zhang
    Abstract:

    A Marine bacterium, designated SCSIO 03483(T), was isolated from a Marine Sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-coloured pigment on nutrient agar medium or Marine agar 2216. Optimal growth occurred in the presence of 0-4 % (w/v) NaCl, at pH 7.0 and a temperature range of 28-37 °C. 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarity with Imtechella halotolerans K1(T) (92.7 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the isolate shared a lineage with members of the genera Imtechella, Joostella and Zhouia. Phospholipids were phosphatidylethanolamine, two unidentified aminolipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω6c/C16 : 1ω7c). The DNA G+C content of strain SCSIO 03483(T) was 38.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483(T) represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Sinomicrobium oceani gen. nov., sp. nov. is proposed. The type strain of Sinobacterium oceani is SCSIO 03483(T) ( = KCTC 23994(T) = CGMCC 1.12145(T)).

Ying Xu - One of the best experts on this subject based on the ideXlab platform.

  • sinomicrobium oceani gen nov sp nov a member of the family flavobacteriaceae isolated from Marine Sediment
    International Journal of Systematic and Evolutionary Microbiology, 2013
    Co-Authors: Xin-peng Tian, Wen-jun Li, Ying Xu, Jie Li, Si Zhang
    Abstract:

    A Marine bacterium, designated SCSIO 03483(T), was isolated from a Marine Sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-coloured pigment on nutrient agar medium or Marine agar 2216. Optimal growth occurred in the presence of 0-4 % (w/v) NaCI, at pH 7.0 and a temperature range of 28-37 degrees C. 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarity with Imtechella halotolerans K1(T) (92.7%). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the isolate shared a lineage with members of the genera Imtechella, Joostella and Zhouia. Phospholipids were phosphatidylethanolamine, two unidentified aminolipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C-15:0, iso-C-17:0 3-OH and summed feature 3 (C-16:1 omega 6c/C-16:1 omega 7c). The DNA G+C content of strain SCSIO 03483(T) was 38.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483(T) represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Sinomicrobium oceani gen. nov., sp. nov. is proposed. The type strain of Sinobacterium oceani is SCSIO 03483(T) (=KCTC 23994(T=)CGMCC 1.12145(T)).

  • Sinomicrobium oceani gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from Marine Sediment.
    International journal of systematic and evolutionary microbiology, 2012
    Co-Authors: Ying Xu, Xin-peng Tian, Wen-jun Li, Jie Li, Si Zhang
    Abstract:

    A Marine bacterium, designated SCSIO 03483(T), was isolated from a Marine Sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-coloured pigment on nutrient agar medium or Marine agar 2216. Optimal growth occurred in the presence of 0-4 % (w/v) NaCl, at pH 7.0 and a temperature range of 28-37 °C. 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarity with Imtechella halotolerans K1(T) (92.7 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the isolate shared a lineage with members of the genera Imtechella, Joostella and Zhouia. Phospholipids were phosphatidylethanolamine, two unidentified aminolipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω6c/C16 : 1ω7c). The DNA G+C content of strain SCSIO 03483(T) was 38.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483(T) represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Sinomicrobium oceani gen. nov., sp. nov. is proposed. The type strain of Sinobacterium oceani is SCSIO 03483(T) ( = KCTC 23994(T) = CGMCC 1.12145(T)).

Wen-jun Li - One of the best experts on this subject based on the ideXlab platform.

  • sinomicrobium oceani gen nov sp nov a member of the family flavobacteriaceae isolated from Marine Sediment
    International Journal of Systematic and Evolutionary Microbiology, 2013
    Co-Authors: Xin-peng Tian, Wen-jun Li, Ying Xu, Jie Li, Si Zhang
    Abstract:

    A Marine bacterium, designated SCSIO 03483(T), was isolated from a Marine Sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-coloured pigment on nutrient agar medium or Marine agar 2216. Optimal growth occurred in the presence of 0-4 % (w/v) NaCI, at pH 7.0 and a temperature range of 28-37 degrees C. 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarity with Imtechella halotolerans K1(T) (92.7%). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the isolate shared a lineage with members of the genera Imtechella, Joostella and Zhouia. Phospholipids were phosphatidylethanolamine, two unidentified aminolipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C-15:0, iso-C-17:0 3-OH and summed feature 3 (C-16:1 omega 6c/C-16:1 omega 7c). The DNA G+C content of strain SCSIO 03483(T) was 38.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483(T) represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Sinomicrobium oceani gen. nov., sp. nov. is proposed. The type strain of Sinobacterium oceani is SCSIO 03483(T) (=KCTC 23994(T=)CGMCC 1.12145(T)).

  • Sinomicrobium oceani gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from Marine Sediment.
    International journal of systematic and evolutionary microbiology, 2012
    Co-Authors: Ying Xu, Xin-peng Tian, Wen-jun Li, Jie Li, Si Zhang
    Abstract:

    A Marine bacterium, designated SCSIO 03483(T), was isolated from a Marine Sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-coloured pigment on nutrient agar medium or Marine agar 2216. Optimal growth occurred in the presence of 0-4 % (w/v) NaCl, at pH 7.0 and a temperature range of 28-37 °C. 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarity with Imtechella halotolerans K1(T) (92.7 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the isolate shared a lineage with members of the genera Imtechella, Joostella and Zhouia. Phospholipids were phosphatidylethanolamine, two unidentified aminolipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω6c/C16 : 1ω7c). The DNA G+C content of strain SCSIO 03483(T) was 38.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483(T) represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Sinomicrobium oceani gen. nov., sp. nov. is proposed. The type strain of Sinobacterium oceani is SCSIO 03483(T) ( = KCTC 23994(T) = CGMCC 1.12145(T)).

Kai Finster - One of the best experts on this subject based on the ideXlab platform.

  • methanobacterium aarhusense sp nov a novel methanogen isolated from a Marine Sediment aarhus bay denmark
    International Journal of Systematic and Evolutionary Microbiology, 2004
    Co-Authors: Adris Georgis Shlimon, Helge Niemann, Niels B. Ramsing, Michael W. Friedrich, Kai Finster
    Abstract:

    Strain H2-LRT, a 5-18 mum long and 0.7 mum wide filamentous, mesophilic, moderately halophilic, non-motile hydrogenotrophic methanogen, was isolated from Marine Sediment of Aarhus Bay, Denmark, 1.7 m below the Sediment surface. On the basis of 16S rRNA gene comparison with sequences of known methanogens, strain H2-LRT could be affiliated to the genus Methanobacterium. The strain forms a distinct line of descent within this genus, with Methanobacterium oryzae (95.9% sequence identity) and Methanobacterium bryantii (95.7% sequence identity) as its closest relatives. The 16S rRNA-based affiliation was supported by comparison of the mcrA gene, which encodes the a-subunit of methyl-coenzyme M reductase. Strain H2-LRT grew only on H-2/CO2. The DNA G+C content is 34.9 mol%. Optimum growth temperature was 45degreesC. The strain grew equally well at pH 7.5 and 8. No growth or methane production was observed below pH 5 or above pH 9. Strain H2-LRT grew well within an NaCl concentration range of 100 and 900 mM. No growth or methane production was observed at 1 M NaCl. At 50 mM NaCl, growth and methane production were reduced. Based on 16S rRNA gene sequence analysis, the isolate is proposed to represent a novel taxon within the genus Methanobacterium, namely Methanobacterium aarhusense sp. nov. The type strain is H2-LRT (=DSM 15219(T)=ATCC BAA-828(T)).

  • biogeochemical and molecular signatures of anaerobic methane oxidation in a Marine Sediment
    Applied and Environmental Microbiology, 2001
    Co-Authors: Trine Rolighed Thomsen, Kai Finster, Niels B. Ramsing
    Abstract:

    Anaerobic methane oxidation was investigated in 6-m-long cores of Marine Sediment from Aarhus Bay, Denmark. Measured concentration profiles for methane and sulfate, as well as in situ rates determined with isotope tracers, indicated that there was a narrow zone of anaerobic methane oxidation about 150 cm below the Sediment surface. Methane could account for 52% of the electron donor requirement for the peak sulfate reduction rate detected in the sulfate-methane transition zone. Molecular signatures of organisms present in the transition zone were detected by using selective PCR primers for sulfate-reducing bacteria and for Archaea. One primer pair amplified the dissimilatory sulfite reductase (DSR) gene of sulfate-reducing bacteria, whereas another primer (ANME) was designed to amplify archaeal sequences found in a recent study of Sediments from the Eel River Basin, as these bacteria have been suggested to be anaerobic methane oxidizers (K. U. Hinrichs, J. M. Hayes, S. P. Sylva, P. G. Brewer, and E. F. DeLong, Nature 398:802–805, 1999). Amplification with the primer pairs produced more amplificate of both target genes with samples from the sulfate-methane transition zone than with samples from the surrounding Sediment. Phylogenetic analysis of the DSR gene sequences retrieved from the transition zone revealed that they all belonged to a novel deeply branching lineage of diverse DSR gene sequences not related to any previously described DSR gene sequence. In contrast, DSR gene sequences found in the top Sediment were related to environmental sequences from other estuarine Sediments and to sequences of members of the genera Desulfonema, Desulfococcus, and Desulfosarcina. Phylogenetic analysis of 16S rRNA sequences obtained with the primers targeting the archaeal group of possible anaerobic methane oxidizers revealed two clusters of ANME sequences, both of which were affiliated with sequences from the Eel River Basin.

Xin-peng Tian - One of the best experts on this subject based on the ideXlab platform.

  • sinomicrobium oceani gen nov sp nov a member of the family flavobacteriaceae isolated from Marine Sediment
    International Journal of Systematic and Evolutionary Microbiology, 2013
    Co-Authors: Xin-peng Tian, Wen-jun Li, Ying Xu, Jie Li, Si Zhang
    Abstract:

    A Marine bacterium, designated SCSIO 03483(T), was isolated from a Marine Sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-coloured pigment on nutrient agar medium or Marine agar 2216. Optimal growth occurred in the presence of 0-4 % (w/v) NaCI, at pH 7.0 and a temperature range of 28-37 degrees C. 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarity with Imtechella halotolerans K1(T) (92.7%). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the isolate shared a lineage with members of the genera Imtechella, Joostella and Zhouia. Phospholipids were phosphatidylethanolamine, two unidentified aminolipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C-15:0, iso-C-17:0 3-OH and summed feature 3 (C-16:1 omega 6c/C-16:1 omega 7c). The DNA G+C content of strain SCSIO 03483(T) was 38.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483(T) represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Sinomicrobium oceani gen. nov., sp. nov. is proposed. The type strain of Sinobacterium oceani is SCSIO 03483(T) (=KCTC 23994(T=)CGMCC 1.12145(T)).

  • Sinomicrobium oceani gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from Marine Sediment.
    International journal of systematic and evolutionary microbiology, 2012
    Co-Authors: Ying Xu, Xin-peng Tian, Wen-jun Li, Jie Li, Si Zhang
    Abstract:

    A Marine bacterium, designated SCSIO 03483(T), was isolated from a Marine Sediment sample collected from the Nansha Islands in the South China Sea. The strain produced roundish colonies with diffusible yellow-coloured pigment on nutrient agar medium or Marine agar 2216. Optimal growth occurred in the presence of 0-4 % (w/v) NaCl, at pH 7.0 and a temperature range of 28-37 °C. 16S rRNA gene sequence analysis indicated that the isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarity with Imtechella halotolerans K1(T) (92.7 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the isolate shared a lineage with members of the genera Imtechella, Joostella and Zhouia. Phospholipids were phosphatidylethanolamine, two unidentified aminolipids and three unknown polar lipids. The major respiratory quinone was MK-6 and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω6c/C16 : 1ω7c). The DNA G+C content of strain SCSIO 03483(T) was 38.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, strain SCSIO 03483(T) represents a novel species in a new genus in the family Flavobacteriaceae, for which the name Sinomicrobium oceani gen. nov., sp. nov. is proposed. The type strain of Sinobacterium oceani is SCSIO 03483(T) ( = KCTC 23994(T) = CGMCC 1.12145(T)).