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Sheng Li - One of the best experts on this subject based on the ideXlab platform.

  • sex identification of the Masked Palm Civet paguma larvata using noninvasive hair samples
    Conservation Genetics Resources, 2016
    Co-Authors: Dan Zhang, Mengyin Xiong, Hongliang Bu, Dajun Wang, Sheng Li, Rongjiang Wang
    Abstract:

    The Masked Palm Civet (Paguma larvata) is one of the most common mesocarnivores in the mountain ecosystem of Southwest China, where mesocarnivores may play critical roles due to loss of apex predators. Given their elusive nature, genetic analysis based on noninvasive survey techniques is a promising approach to determine the population dynamics and ecological functions of Masked Palm Civets. Here we describe a method for molecular sex identification of Masked Palm Civets. Two pairs of primers were designed to amplify fragments of zfx and sry genes, respectively. Through PCR amplification, the expected fragments, two (123 and 244 bp) in males and one (123 bp) in females, were successfully obtained in 8 sex-known hair samples. The procedures we developed here represent a reliable method for sex identification of Masked Palm Civets.

  • Spatial Co-occurrence and activity patterns of mesocarnivores in the temperate forests of Southwest China
    PLoS ONE, 2016
    Co-Authors: Hongliang Bu, William J Mcshea, Zhi Lu, Dajun Wang, Fang Wang, Sheng Li
    Abstract:

    Understanding the interactions between species and their coexistence mechanisms will help explain biodiversity maintenance and enable managers to make sound conservation decisions. Mesocarnivores are abundant and diverse mid-sized carnivores and can have profound impacts on the function, structure and dynamics of ecosystem after the extirpation of apex predators in many ecosystems. The moist temperate forests of Southwest China harbor a diverse community of mesocarnivores in the absence of apex predators. Sympatric species tend to partition limited resources along time, diet and space to facilitate coexistence. We determined the spatial and temporal patterns for five species of mesocarnivores. We used detection histories from a large camera-trap dataset collected from 2004–2015 with an extensive effort of 23,313 camera-days from 495 camera locations. The five mesocarnivore species included Masked Palm Civet Paguma larvata, leopard cat Prionailurus bengalensis, hog badger Arctonyx collaris, yellow-throated marten Martes flavigula, and Siberian weasel Mustela sibirica. Only the Masked Palm Civet and hog badger tended to avoid each other; while for other pairs of species, they occurred independently of each other, or no clear pattern observed. With regard to seasonal activity, yellow-throated marten was most active in winter, opposite the pattern observed for Masked Palm Civet, leopard cat and hog badger. For diel activity, Masked Palm Civet, leopard cat and hog badger were primarily nocturnal and crepuscular; yellow-throated marten was diurnal, and Siberian weasel had no clear pattern for most of the year (March to November), but was nocturnal in the winter (December to February). The seasonal shift of the Siberian weasel may be due to the high diet overlap among species in winter. Our results provided new facts and insights into this unique community of mesocarnivores of southwest China, and will facilitate future studies on the mechanism determining coexistence of animal species within complex system.

Li Sheng - One of the best experts on this subject based on the ideXlab platform.

  • Sex identification of the Masked Palm Civet (Paguma larvata) using noninvasive hair samples
    CONSERVATION GENETICS RESOURCES, 2016
    Co-Authors: Zhang Dan, Bu Hongliang, Xiong Mengyin, Wang Dajun, Li Sheng, Yao Meng, Wang Rongjiang
    Abstract:

    The Masked Palm Civet (Paguma larvata) is one of the most common mesocarnivores in the mountain ecosystem of Southwest China, where mesocarnivores may play critical roles due to loss of apex predators. Given their elusive nature, genetic analysis based on noninvasive survey techniques is a promising approach to determine the population dynamics and ecological functions of Masked Palm Civets. Here we describe a method for molecular sex identification of Masked Palm Civets. Two pairs of primers were designed to amplify fragments of zfx and sry genes, respectively. Through PCR amplification, the expected fragments, two (123 and 244 bp) in males and one (123 bp) in females, were successfully obtained in 8 sex-known hair samples. The procedures we developed here represent a reliable method for sex identification of Masked Palm Civets.Nature Conservancy China; Sichuan Nature Conservation FoundationSCI(E)ARTICLErjwang@pku.edu.cn3207-209

  • Spatial Co-Occurrence and Activity Patterns of Mesocarnivores in the Temperate Forests of Southwest China
    PLOS ONE, 2016
    Co-Authors: Bu Hongliang, Wang Dajun, Wang Fang, Mcshea, William J., Lu Zhi, Li Sheng
    Abstract:

    Understanding the interactions between species and their coexistence mechanisms will help explain biodiversity maintenance and enable managers to make sound conservation decisions. Mesocarnivores are abundant and diverse mid-sized carnivores and can have profound impacts on the function, structure and dynamics of ecosystem after the extirpation of apex predators in many ecosystems. The moist temperate forests of Southwest China harbor a diverse community of mesocarnivores in the absence of apex predators. Sympatric species tend to partition limited resources along time, diet and space to facilitate coexistence. We determined the spatial and temporal patterns for five species of mesocarnivores. We used detection histories from a large camera-trap dataset collected from 2004-2015 with an extensive effort of 23,313 camera-days from 495 camera locations. The five mesocarnivore species included Masked Palm Civet Paguma larvata, leopard cat Prionailurus bengalensis, hog badger Arctonyx collaris, yellow-throated marten Martes flavigula, and Siberian weasel Mustela sibirica. Only the Masked Palm Civet and hog badger tended to avoid each other; while for other pairs of species, they occurred independently of each other, or no clear pattern observed. With regard to seasonal activity, yellow-throated marten was most active in winter, opposite the pattern observed for Masked Palm Civet, leopard cat and hog badger. For diel activity, Masked Palm Civet, leopard cat and hog badger were primarily nocturnal and crepuscular; yellow-throated marten was diurnal, and Siberian weasel had no clear pattern for most of the year (March to November), but was nocturnal in the winter (December to February). The seasonal shift of the Siberian weasel may be due to the high diet overlap among species in winter. Our results provided new facts and insights into this unique community of mesocarnivores of southwest China, and will facilitate future studies on the mechanism determining coexistence of animal species within complex system.Sichuan Nature Conservancy Foundation [SNCF/PU092013]SCI(E)PubMedARTICLEdjwang@pku.edu.cn; shengli@pku.edu.cn10e01642711

Hongliang Bu - One of the best experts on this subject based on the ideXlab platform.

  • the complete mitochondrial genome of the Masked Palm Civet paguma larvata mammalia carnivora
    Mitochondrial DNA, 2016
    Co-Authors: Dan Zhang, Hongliang Bu, Liwen Xu, Chongren Xu, Di Wang, Rongjiang Wang
    Abstract:

    The complete mitochondrial genome of the Masked Palm Civet (Paguma larvata, Mammalia, Carnivora) is a circular molecule of 16 710 bp in length, containing 22 transfer RNA genes, 13 protein-coding genes, two ribosomal RNA genes, and a control region. The features of the mitochondrial genome of the Masked Palm Civet are similar to the other mammals. The phylogenetic analysis shows that all species from the family Viverridae cluster together, in which P. larvata exhibits the closest relationship with Genetta servalina.

  • sex identification of the Masked Palm Civet paguma larvata using noninvasive hair samples
    Conservation Genetics Resources, 2016
    Co-Authors: Dan Zhang, Mengyin Xiong, Hongliang Bu, Dajun Wang, Sheng Li, Rongjiang Wang
    Abstract:

    The Masked Palm Civet (Paguma larvata) is one of the most common mesocarnivores in the mountain ecosystem of Southwest China, where mesocarnivores may play critical roles due to loss of apex predators. Given their elusive nature, genetic analysis based on noninvasive survey techniques is a promising approach to determine the population dynamics and ecological functions of Masked Palm Civets. Here we describe a method for molecular sex identification of Masked Palm Civets. Two pairs of primers were designed to amplify fragments of zfx and sry genes, respectively. Through PCR amplification, the expected fragments, two (123 and 244 bp) in males and one (123 bp) in females, were successfully obtained in 8 sex-known hair samples. The procedures we developed here represent a reliable method for sex identification of Masked Palm Civets.

  • Spatial Co-occurrence and activity patterns of mesocarnivores in the temperate forests of Southwest China
    PLoS ONE, 2016
    Co-Authors: Hongliang Bu, William J Mcshea, Zhi Lu, Dajun Wang, Fang Wang, Sheng Li
    Abstract:

    Understanding the interactions between species and their coexistence mechanisms will help explain biodiversity maintenance and enable managers to make sound conservation decisions. Mesocarnivores are abundant and diverse mid-sized carnivores and can have profound impacts on the function, structure and dynamics of ecosystem after the extirpation of apex predators in many ecosystems. The moist temperate forests of Southwest China harbor a diverse community of mesocarnivores in the absence of apex predators. Sympatric species tend to partition limited resources along time, diet and space to facilitate coexistence. We determined the spatial and temporal patterns for five species of mesocarnivores. We used detection histories from a large camera-trap dataset collected from 2004–2015 with an extensive effort of 23,313 camera-days from 495 camera locations. The five mesocarnivore species included Masked Palm Civet Paguma larvata, leopard cat Prionailurus bengalensis, hog badger Arctonyx collaris, yellow-throated marten Martes flavigula, and Siberian weasel Mustela sibirica. Only the Masked Palm Civet and hog badger tended to avoid each other; while for other pairs of species, they occurred independently of each other, or no clear pattern observed. With regard to seasonal activity, yellow-throated marten was most active in winter, opposite the pattern observed for Masked Palm Civet, leopard cat and hog badger. For diel activity, Masked Palm Civet, leopard cat and hog badger were primarily nocturnal and crepuscular; yellow-throated marten was diurnal, and Siberian weasel had no clear pattern for most of the year (March to November), but was nocturnal in the winter (December to February). The seasonal shift of the Siberian weasel may be due to the high diet overlap among species in winter. Our results provided new facts and insights into this unique community of mesocarnivores of southwest China, and will facilitate future studies on the mechanism determining coexistence of animal species within complex system.

Bu Hongliang - One of the best experts on this subject based on the ideXlab platform.

  • The complete mitochondrial genome of the Masked Palm Civet (Paguma larvata, Mammalia, Carnivora)
    MITOCHONDRIAL DNA, 2016
    Co-Authors: Zhang Dan, Di Wang, Xu Liwen, Bu Hongliang, Xu Chongren, Wang Rongjiang
    Abstract:

    The complete mitochondrial genome of the Masked Palm Civet (Paguma larvata, Mammalia, Carnivora) is a circular molecule of 16 710 bp in length, containing 22 transfer RNA genes, 13 protein-coding genes, two ribosomal RNA genes, and a control region. The features of the mitochondrial genome of the Masked Palm Civet are similar to the other mammals. The phylogenetic analysis shows that all species from the family Viverridae cluster together, in which P. larvata exhibits the closest relationship with Genetta servalina.Nature Conservation; Sichuan Nature Conservation FoundationSCI(E)PubMedARTICLErjwang@pku.edu.cn53764-37652

  • Sex identification of the Masked Palm Civet (Paguma larvata) using noninvasive hair samples
    CONSERVATION GENETICS RESOURCES, 2016
    Co-Authors: Zhang Dan, Bu Hongliang, Xiong Mengyin, Wang Dajun, Li Sheng, Yao Meng, Wang Rongjiang
    Abstract:

    The Masked Palm Civet (Paguma larvata) is one of the most common mesocarnivores in the mountain ecosystem of Southwest China, where mesocarnivores may play critical roles due to loss of apex predators. Given their elusive nature, genetic analysis based on noninvasive survey techniques is a promising approach to determine the population dynamics and ecological functions of Masked Palm Civets. Here we describe a method for molecular sex identification of Masked Palm Civets. Two pairs of primers were designed to amplify fragments of zfx and sry genes, respectively. Through PCR amplification, the expected fragments, two (123 and 244 bp) in males and one (123 bp) in females, were successfully obtained in 8 sex-known hair samples. The procedures we developed here represent a reliable method for sex identification of Masked Palm Civets.Nature Conservancy China; Sichuan Nature Conservation FoundationSCI(E)ARTICLErjwang@pku.edu.cn3207-209

  • Spatial Co-Occurrence and Activity Patterns of Mesocarnivores in the Temperate Forests of Southwest China
    PLOS ONE, 2016
    Co-Authors: Bu Hongliang, Wang Dajun, Wang Fang, Mcshea, William J., Lu Zhi, Li Sheng
    Abstract:

    Understanding the interactions between species and their coexistence mechanisms will help explain biodiversity maintenance and enable managers to make sound conservation decisions. Mesocarnivores are abundant and diverse mid-sized carnivores and can have profound impacts on the function, structure and dynamics of ecosystem after the extirpation of apex predators in many ecosystems. The moist temperate forests of Southwest China harbor a diverse community of mesocarnivores in the absence of apex predators. Sympatric species tend to partition limited resources along time, diet and space to facilitate coexistence. We determined the spatial and temporal patterns for five species of mesocarnivores. We used detection histories from a large camera-trap dataset collected from 2004-2015 with an extensive effort of 23,313 camera-days from 495 camera locations. The five mesocarnivore species included Masked Palm Civet Paguma larvata, leopard cat Prionailurus bengalensis, hog badger Arctonyx collaris, yellow-throated marten Martes flavigula, and Siberian weasel Mustela sibirica. Only the Masked Palm Civet and hog badger tended to avoid each other; while for other pairs of species, they occurred independently of each other, or no clear pattern observed. With regard to seasonal activity, yellow-throated marten was most active in winter, opposite the pattern observed for Masked Palm Civet, leopard cat and hog badger. For diel activity, Masked Palm Civet, leopard cat and hog badger were primarily nocturnal and crepuscular; yellow-throated marten was diurnal, and Siberian weasel had no clear pattern for most of the year (March to November), but was nocturnal in the winter (December to February). The seasonal shift of the Siberian weasel may be due to the high diet overlap among species in winter. Our results provided new facts and insights into this unique community of mesocarnivores of southwest China, and will facilitate future studies on the mechanism determining coexistence of animal species within complex system.Sichuan Nature Conservancy Foundation [SNCF/PU092013]SCI(E)PubMedARTICLEdjwang@pku.edu.cn; shengli@pku.edu.cn10e01642711

Deng Fei - One of the best experts on this subject based on the ideXlab platform.

  • Angiotensin-converting enzyme 2 (ACE2) from raccoon dog can serve as an efficient receptor for the spike protein of severe acute respiratory syndrome coronavirus
    journal of general virology, 2009
    Co-Authors: Xu Lili, Zhang Yanfang, Liu Yun, Chen Zhiwei, Deng Hongkui, Ma Zhongbin, Wang Hualin, Hu Zhihong, Deng Fei
    Abstract:

    Raccoon dog is one of the suspected intermediate hosts of severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, the angiotensin-converting enzyme 2 (ACE2) gene of raccoon dog (rdACE2) was cloned and sequenced. The amino acid sequence of rdACE2 has identities of 99.3, 89.2, 83.9 and 80.4% to ACE2 proteins from dog, Masked Palm Civet (pcACE2), human (huACE2) and bat, respectively. There are six amino acid changes in rdACE2 compared with huACE2, and four changes compared with pcACE2, within the 18 residues of ACE2 known to make direct contact with the SARS-CoV S protein. A HeLa cell line stably expressing rdACE2 was established; Western blot analyses and an enzyme-activity assay indicated that the cell line expressed ACE2 at a similar level to two previously established cell lines that express ACE2 from human and Masked Palm Civet, respectively. Human immunodeficiency virus-backboned pseudoviruses expressing spike proteins derived from human SARS-CoV or SARS-CoV-like viruses of Masked Palm Civets and raccoon dogs were tested for their entry efficiency into these cell lines. The results showed that rdACE2 is a more efficient receptor for human SARS-CoV, but not for SARS-CoV-like viruses of Masked Palm Civets and raccoon dogs, than huACE2 or pcACE2. This study provides useful data to elucidate the role of raccoon dog in SARS outbreaks.Biotechnology & Applied MicrobiologyVirologySCI(E)2ARTICLE2695-27039