The Experts below are selected from a list of 57 Experts worldwide ranked by ideXlab platform

Imad About - One of the best experts on this subject based on the ideXlab platform.

  • human tooth culture a study model for reparative dentinogenesis and direct pulp capping Materials Biocompatibility
    Journal of Biomedical Materials Research Part B, 2008
    Co-Authors: Odile Tecles, Patrick Laurent, Virginie Aubut, Imad About
    Abstract:

    In a previous work, based on an in vitro entire tooth culture model of human immature third molars, we demonstrated that perivascular progenitor cells can proliferate and migrate to the injury site after pulp exposure. In this work, we investigated the differentiation of cells after direct capping with bioMaterials classically used in restorative dentistry. Histological staining after direct pulp capping with Calcium Hydroxide XR® or MTA revealed early and progressive mineralized foci formation containing BrdU-labeled sequestered cells. The molecular characterization of the matrix and the sequestered cells by immunohistochemistry (Collagene type I, Dentin sialoprotein, and Nestin) clearly demonstrates that these areas share common characteristics of the mineralized matrix of reparative dentin formed by odontoblast-like cells. This reproduces some features of the pulp responses after applying these Materials in vivo and demonstrates that the entire tooth culture model reproduces a part of the early steps of dentin regeneration in vivo. Its future development may be useful in studying the effects of bioMaterials on this process. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008

  • human tooth culture a study model for reparative dentinogenesis and direct pulp capping Materials Biocompatibility
    Journal of Biomedical Materials Research Part B, 2008
    Co-Authors: Odile Tecles, Patrick Laurent, Virginie Aubut, Imad About
    Abstract:

    In a previous work, based on an in vitro entire tooth culture model of human immature third molars, we demonstrated that perivascular progenitor cells can proliferate and migrate to the injury site after pulp exposure. In this work, we investigated the differentiation of cells after direct capping with bioMaterials classically used in restorative dentistry. Histological staining after direct pulp capping with Calcium Hydroxide XR(R) or MTA revealed early and progressive mineralized foci formation containing BrdU-labeled sequestered cells. The molecular characterization of the matrix and the sequestered cells by immunohistochemistry (Collagene type I, Dentin sialoprotein, and Nestin) clearly demonstrates that these areas share common characteristics of the mineralized matrix of reparative dentin formed by odontoblast-like cells. This reproduces some features of the pulp responses after applying these Materials in vivo and demonstrates that the entire tooth culture model reproduces a part of the early steps of dentin regeneration in vivo. Its future development may be useful in studying the effects of bioMaterials on this process.

Odile Tecles - One of the best experts on this subject based on the ideXlab platform.

  • human tooth culture a study model for reparative dentinogenesis and direct pulp capping Materials Biocompatibility
    Journal of Biomedical Materials Research Part B, 2008
    Co-Authors: Odile Tecles, Patrick Laurent, Virginie Aubut, Imad About
    Abstract:

    In a previous work, based on an in vitro entire tooth culture model of human immature third molars, we demonstrated that perivascular progenitor cells can proliferate and migrate to the injury site after pulp exposure. In this work, we investigated the differentiation of cells after direct capping with bioMaterials classically used in restorative dentistry. Histological staining after direct pulp capping with Calcium Hydroxide XR® or MTA revealed early and progressive mineralized foci formation containing BrdU-labeled sequestered cells. The molecular characterization of the matrix and the sequestered cells by immunohistochemistry (Collagene type I, Dentin sialoprotein, and Nestin) clearly demonstrates that these areas share common characteristics of the mineralized matrix of reparative dentin formed by odontoblast-like cells. This reproduces some features of the pulp responses after applying these Materials in vivo and demonstrates that the entire tooth culture model reproduces a part of the early steps of dentin regeneration in vivo. Its future development may be useful in studying the effects of bioMaterials on this process. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008

  • human tooth culture a study model for reparative dentinogenesis and direct pulp capping Materials Biocompatibility
    Journal of Biomedical Materials Research Part B, 2008
    Co-Authors: Odile Tecles, Patrick Laurent, Virginie Aubut, Imad About
    Abstract:

    In a previous work, based on an in vitro entire tooth culture model of human immature third molars, we demonstrated that perivascular progenitor cells can proliferate and migrate to the injury site after pulp exposure. In this work, we investigated the differentiation of cells after direct capping with bioMaterials classically used in restorative dentistry. Histological staining after direct pulp capping with Calcium Hydroxide XR(R) or MTA revealed early and progressive mineralized foci formation containing BrdU-labeled sequestered cells. The molecular characterization of the matrix and the sequestered cells by immunohistochemistry (Collagene type I, Dentin sialoprotein, and Nestin) clearly demonstrates that these areas share common characteristics of the mineralized matrix of reparative dentin formed by odontoblast-like cells. This reproduces some features of the pulp responses after applying these Materials in vivo and demonstrates that the entire tooth culture model reproduces a part of the early steps of dentin regeneration in vivo. Its future development may be useful in studying the effects of bioMaterials on this process.

Patrick Laurent - One of the best experts on this subject based on the ideXlab platform.

  • human tooth culture a study model for reparative dentinogenesis and direct pulp capping Materials Biocompatibility
    Journal of Biomedical Materials Research Part B, 2008
    Co-Authors: Odile Tecles, Patrick Laurent, Virginie Aubut, Imad About
    Abstract:

    In a previous work, based on an in vitro entire tooth culture model of human immature third molars, we demonstrated that perivascular progenitor cells can proliferate and migrate to the injury site after pulp exposure. In this work, we investigated the differentiation of cells after direct capping with bioMaterials classically used in restorative dentistry. Histological staining after direct pulp capping with Calcium Hydroxide XR® or MTA revealed early and progressive mineralized foci formation containing BrdU-labeled sequestered cells. The molecular characterization of the matrix and the sequestered cells by immunohistochemistry (Collagene type I, Dentin sialoprotein, and Nestin) clearly demonstrates that these areas share common characteristics of the mineralized matrix of reparative dentin formed by odontoblast-like cells. This reproduces some features of the pulp responses after applying these Materials in vivo and demonstrates that the entire tooth culture model reproduces a part of the early steps of dentin regeneration in vivo. Its future development may be useful in studying the effects of bioMaterials on this process. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008

  • human tooth culture a study model for reparative dentinogenesis and direct pulp capping Materials Biocompatibility
    Journal of Biomedical Materials Research Part B, 2008
    Co-Authors: Odile Tecles, Patrick Laurent, Virginie Aubut, Imad About
    Abstract:

    In a previous work, based on an in vitro entire tooth culture model of human immature third molars, we demonstrated that perivascular progenitor cells can proliferate and migrate to the injury site after pulp exposure. In this work, we investigated the differentiation of cells after direct capping with bioMaterials classically used in restorative dentistry. Histological staining after direct pulp capping with Calcium Hydroxide XR(R) or MTA revealed early and progressive mineralized foci formation containing BrdU-labeled sequestered cells. The molecular characterization of the matrix and the sequestered cells by immunohistochemistry (Collagene type I, Dentin sialoprotein, and Nestin) clearly demonstrates that these areas share common characteristics of the mineralized matrix of reparative dentin formed by odontoblast-like cells. This reproduces some features of the pulp responses after applying these Materials in vivo and demonstrates that the entire tooth culture model reproduces a part of the early steps of dentin regeneration in vivo. Its future development may be useful in studying the effects of bioMaterials on this process.

Virginie Aubut - One of the best experts on this subject based on the ideXlab platform.

  • human tooth culture a study model for reparative dentinogenesis and direct pulp capping Materials Biocompatibility
    Journal of Biomedical Materials Research Part B, 2008
    Co-Authors: Odile Tecles, Patrick Laurent, Virginie Aubut, Imad About
    Abstract:

    In a previous work, based on an in vitro entire tooth culture model of human immature third molars, we demonstrated that perivascular progenitor cells can proliferate and migrate to the injury site after pulp exposure. In this work, we investigated the differentiation of cells after direct capping with bioMaterials classically used in restorative dentistry. Histological staining after direct pulp capping with Calcium Hydroxide XR® or MTA revealed early and progressive mineralized foci formation containing BrdU-labeled sequestered cells. The molecular characterization of the matrix and the sequestered cells by immunohistochemistry (Collagene type I, Dentin sialoprotein, and Nestin) clearly demonstrates that these areas share common characteristics of the mineralized matrix of reparative dentin formed by odontoblast-like cells. This reproduces some features of the pulp responses after applying these Materials in vivo and demonstrates that the entire tooth culture model reproduces a part of the early steps of dentin regeneration in vivo. Its future development may be useful in studying the effects of bioMaterials on this process. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008

  • human tooth culture a study model for reparative dentinogenesis and direct pulp capping Materials Biocompatibility
    Journal of Biomedical Materials Research Part B, 2008
    Co-Authors: Odile Tecles, Patrick Laurent, Virginie Aubut, Imad About
    Abstract:

    In a previous work, based on an in vitro entire tooth culture model of human immature third molars, we demonstrated that perivascular progenitor cells can proliferate and migrate to the injury site after pulp exposure. In this work, we investigated the differentiation of cells after direct capping with bioMaterials classically used in restorative dentistry. Histological staining after direct pulp capping with Calcium Hydroxide XR(R) or MTA revealed early and progressive mineralized foci formation containing BrdU-labeled sequestered cells. The molecular characterization of the matrix and the sequestered cells by immunohistochemistry (Collagene type I, Dentin sialoprotein, and Nestin) clearly demonstrates that these areas share common characteristics of the mineralized matrix of reparative dentin formed by odontoblast-like cells. This reproduces some features of the pulp responses after applying these Materials in vivo and demonstrates that the entire tooth culture model reproduces a part of the early steps of dentin regeneration in vivo. Its future development may be useful in studying the effects of bioMaterials on this process.

Ružica S Nikolic - One of the best experts on this subject based on the ideXlab platform.

  • potentiometric stripping analysis of zinc and copper in human teeth and dental Materials
    Journal of Trace Elements in Medicine and Biology, 2008
    Co-Authors: Biljana M Kalicanin, Ružica S Nikolic
    Abstract:

    Abstract Potentiometric stripping analysis (PSA) with oxygen as the oxidant has been used to determine soluble zinc and copper levels in exfoliated human teeth (all of which required extraction for orthodontic reasons) and commercial dental Materials. The soluble zinc and copper contents of teeth were slightly below the zinc and copper contents in whole teeth reported by other researchers, except in the case of tooth with removed amalgam filling. Soluble zinc and copper concentrations of the dental Materials and metalceramic crowns were 0.50–6.30, and of 2.00–4.30 μg/g, respectively. The results of this work suggest that PSA may be a good method for zinc and copper leaching studies during the investigation of dental prosthetic Materials’ Biocompatibility. Corrosive action of acidic media as evidenced by SEM micrographs caused the leaching of metal ions from teeth.